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KLHL9 (NM_018847) Human Tagged ORF Clone
CAT＃: RC203728
- TrueORF®
KLHL9 (Myc-DDK-tagged)-Human kelch-like 9 (Drosophila) (KLHL9)
ORF Plasmid: tGFP
"NM_018847" in other vectors (4)
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CNY 4,592.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC203728 representing NM_018847
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAGTGTCCCTTGGTAACGGCGAAATGGGCGTCTCTGCCCATTTGCAGCCTTGTAAGGCAGGAACCA CACGCTTTTTTACCAGCAATACTCACAGTTCGGTGGTATTGCAAGGCTTTGATCAGCTTAGAATAGAAGG ATTGCTTTGTGATGTGACCCTGGTACCAGGTGATGGAGATGAAATCTTCCCTGTTCACAGAGCTATGATG GCGTCTGCTAGTGATTATTTCAAAGCCATGTTCACAGGTGGAATGAAAGAACAAGATTTGATGTGCATTA AGCTTCATGGGGTGAACAAGGTTGGTCTGAAGAAAATCATTGATTTTATTTATACTGCAAAACTTTCTCT TAATATGGACAATCTTCAGGACACACTTGAAGCTGCTAGCTTTTTACAAATATTACCCGTTTTGGATTTC TGTAAAGTATTTCTTATATCAGGAGTCTCTTTGGATAACTGTGTTGAGGTTGGACGAATTGCTAACACCT ACAATCTTATAGAAGTGGATAAATATGTTAATAATTTCATCCTGAAGAACTTTCCTGCTTTATTGAGTAC TGGGGAGTTTCTAAAACTCCCTTTTGAACGACTTGCATTTGTGCTTTCCAGTAATAGTCTTAAGCACTGT ACCGAACTTGAACTCTTTAAGGCAGCCTGTCGCTGGCTAAGGTTGGAAGACCCTCGGATGGATTATGCTG CAAAGTTAATGAAGAATATTCGATTTCCACTGATGACACCACAGGATCTCATCAATTACGTGCAGACAGT AGATTTCATGAGAACAGACAATACCTGCGTGAATTTGCTTTTGGAAGCTAGCAATTACCAAATGATGCCA TATATGCAGCCAGTGATGCAGTCAGATAGAACTGCCATTCGATCTGACTCCACTCACTTGGTTACATTAG GAGGAGTTTTGAGGCAGCAGCTGGTTGTCAGTAAAGAATTACGGATGTATGATGAAAGGGCACAAGAATG GAGATCTTTAGCCCCAATGGATGCTCCCCGTTACCAGCATGGTATTGCTGTCATTGGAAACTTTCTTTAT GTAGTTGGTGGTCAGAGTAATTATGATACAAAAGGAAAAACTGCTGTTGATACAGTTTTCAGATTTGATC CTCGGTATAATAAATGGATGCAGGTTGCATCATTAAATGAAAAGCGCACATTCTTTCACTTGAGTGCCCT CAAAGGACATTTGTATGCAGTTGGTGGGCGCAGTGCAGCTGGTGAACTGGCCACAGTAGAATGTTACAAC CCAAGAATGAATGAGTGGAGCTATGTTGCAAAAATGAGTGAACCCCACTATGGTCATGCTGGAACAGTAT ATGGAGGCTTAATGTATATTTCAGGAGGAATTACCCATGACACTTTCCAAAATGAGCTCATGTGTTTTGA CCCAGATACAGATAAATGGATGCAAAAGGCTCCAATGACTACAGTCAGAGGTCTGCATTGCATGTGTACA GTTGGAGATAAGCTCTATGTCATTGGTGGCAATCACTTCAGAGGAACAAGTGATTATGATGATGTTCTAA GCTGTGAATACTATTCACCAACCCTTGACCAGTGGACCCCAATTGCCGCCATGTTAAGAGGCCAAAGTGA TGTTGGAGTTGCCGTCTTTGAAAATAAAATCTATGTTGTTGGTGGATATTCTTGGAATAATCGTTGTATG GTAGAAATTGTCCAGAAATATGACCCAGAAAAAGATGAGTGGCATAAAGTTTTTGATCTTCCAGAGTCAC TTGGTGGCATTCGAGCCTGTACACTCACAGTTTTTCCACCTGAAGAAAACCCTGGGTCACCTTCTAGAGA ATCACCTCTTTCAGCACCTTCAGATCATTCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203728 representing NM_018847
Red=Cloning site Green=Tags(s) MKVSLGNGEMGVSAHLQPCKAGTTRFFTSNTHSSVVLQGFDQLRIEGLLCDVTLVPGDGDEIFPVHRAMM ASASDYFKAMFTGGMKEQDLMCIKLHGVNKVGLKKIIDFIYTAKLSLNMDNLQDTLEAASFLQILPVLDF CKVFLISGVSLDNCVEVGRIANTYNLIEVDKYVNNFILKNFPALLSTGEFLKLPFERLAFVLSSNSLKHC TELELFKAACRWLRLEDPRMDYAAKLMKNIRFPLMTPQDLINYVQTVDFMRTDNTCVNLLLEASNYQMMP YMQPVMQSDRTAIRSDSTHLVTLGGVLRQQLVVSKELRMYDERAQEWRSLAPMDAPRYQHGIAVIGNFLY VVGGQSNYDTKGKTAVDTVFRFDPRYNKWMQVASLNEKRTFFHLSALKGHLYAVGGRSAAGELATVECYN PRMNEWSYVAKMSEPHYGHAGTVYGGLMYISGGITHDTFQNELMCFDPDTDKWMQKAPMTTVRGLHCMCT VGDKLYVIGGNHFRGTSDYDDVLSCEYYSPTLDQWTPIAAMLRGQSDVGVAVFENKIYVVGGYSWNNRCM VEIVQKYDPEKDEWHKVFDLPESLGGIRACTLTVFPPEENPGSPSRESPLSAPSDHS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_018847 |
| ORF Size | 1851 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_018847.4 |
| RefSeq Size | 4412 bp |
| RefSeq ORF | 1854 bp |
| Locus ID | 55958 |
| UniProt ID | Q9P2J3 |
| Domains | BTB, Kelch |
| Protein Pathways | Ubiquitin mediated proteolysis |
| MW | 69.2 kDa |
| Gene Summary | This gene encodes a protein that belongs to the kelch repeat-containing family, and contains an N-terminal BTB/POZ domain, a BACK domain and six C-terminal kelch repeats. The encoded protein is a component of a complex with cullin 3-based E3 ligase, which plays a role in mitosis. This protein complex is a cell cycle regulator, and functions in the organization and integrity of the spindle midzone in anaphase and the completion of cytokinesis. The complex is required for the removal of the chromosomal passenger protein aurora B from mitotic chromosomes. [provided by RefSeq, Jul 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203728L3 | Lenti-ORF clone of KLHL9 (Myc-DDK-tagged)-Human kelch-like 9 (Drosophila) (KLHL9) |
CNY 6,460.00 |
|
| RC203728L4 | Lenti-ORF clone of KLHL9 (mGFP-tagged)-Human kelch-like 9 (Drosophila) (KLHL9) |
CNY 6,460.00 |
|
| RG203728 | KLHL9 (tGFP-tagged) - Human kelch-like 9 (Drosophila) (KLHL9) |
CNY 5,040.00 |
|
| SC100463 | KLHL9 (untagged)-Human kelch-like 9 (Drosophila) (KLHL9) |
CNY 4,600.00 |
