ADCK4 (COQ8B) (NM_024876) Human Tagged ORF Clone

CAT#: RC203700

ADCK4 (Myc-DDK-tagged)-Human aarF domain containing kinase 4 (ADCK4), transcript variant 1

ORF Plasmid: DDK tGFP



  "NM_024876" in other vectors (8)

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CNY 4,048.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit polyclonal anti-ADCK4 antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms ADCK4; NPHS9
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC203700 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGGCTGAAGGTGGGGGGCCTACTTCGGGGGACCGGTGGACAGCTGGGCCAGACTGTTGGTTGGCCTT
GTGGGGCCCTGGGGCCTGGGCCCCACCGCTGGGGACCATGTGGAGGTTCTTGGGCCCAAAAGTTTTACCA
GGATGGGCCTGGGAGAGGCCTGGGTGAGGAGGACATTCGCAGGGCACGGGAGGCCCGTCCCAGGAAGACA
CCCCGGCCCCAGCTGAGTGACCGCTCTCGAGAACGCAAGGTGCCTGCCTCCCGCATCAGCCGCTTGGCCA
ACTTTGGGGGACTGGCTGTGGGCTTGGGGCTAGGAGTACTGGCCGAGATGGCTAAGAAGTCCATGCCAGG
AGGTCGTCTGCAGTCAGAGGGTGGTTCTGGGCTGGACTCCAGCCCCTTCCTGTCGGAGGCCAATGCCGAG
CGGATTGTGCAGACCTTATGTACAGTTCGAGGGGCCGCCCTCAAGGTTGGCCAGATGCTCAGCATCCAGG
ACAACAGCTTCATCAGCCCTCAGCTGCAGCACATCTTTGAGCGGGTCCGCCAGAGCGCCGACTTCATGCC
CCGCTGGCAGATGCTGAGAGTTCTTGAAGAGGAGCTCGGCAGGGACTGGCAGGCCAAGGTGGCCTCCTTG
GAGGAGGTGCCCTTTGCCGCTGCCTCAATTGGGCAGGTGCACCAGGGCCTGCTGAGGGACGGGACGGAGG
TGGCCGTGAAGATCCAGTACCCCGGCATAGCCCAGAGCATTCAGAGCGATGTCCAGAACCTGCTGGCGGT
ACTCAAGATGAGCGCGGCCCTGCCCGCGGGCCTGTTTGCCGAGCAGAGCCTGCAGGCCTTGCAGCAGGAG
CTGGCTTGGGAGTGTGACTACCGTCGTGAGGCGGCTTGTGCCCAGAATTTCAGGCAGCTGCTGGCAAATG
ACCCCTTCTTCCGGGTCCCAGCCGTGGTTAAGGAGCTGTGCACGACACGGGTGCTGGGCATGGAGCTGGC
TGGAGGGGTCCCCCTGGACCAGTGCCAGGGCCTAAGCCAGGACCTGCGGAACCAGATTTGCTTCCAGCTC
CTGACGCTGTGTCTGCGGGAGCTGTTTGAGTTCCGATTCATGCAGACTGACCCCAACTGGGCCAACTTCC
TGTATGATGCCTCCAGCCACCAGGTGACCCTGCTGGACTTTGGTGCAAGCCGGGAGTTTGGGACAGAGTT
CACAGACCATTACATCGAGGTGGTGAAGGCTGCAGCTGATGGAGACAGAGACTGTGTCCTGCAGAAGTCC
AGGGACCTCAAATTCCTCACAGGCTTTGAAACCAAGGCATTCTCCGACGCCCACGTGGAGGCAGTGATGA
TCCTGGGGGAGCCTTTCGCCACCCAGGGCCCTTACGACTTTGGGTCGGGGGAAACGGCCCGCCGCATACA
GGACCTCATCCCGGTGCTGCTGCGGCACCGGCTGTGTCCCCCACCCGAGGAGACCTATGCCCTGCACCGC
AAGCTGGCAGGGGCTTTCCTGGCCTGTGCCCACCTCCGAGCCCACATCGCCTGCAGGGACCTCTTCCAGG
ACACCTACCACCGCTACTGGGCCAGTCGCCAGCCAGACGCAGCCACTGCCGGCAGCCTCCCCACCAAAGG
GGACTCCTGGGTGGATCCCTCA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC203700 protein sequence
Red=Cloning site Green=Tags(s)

MWLKVGGLLRGTGGQLGQTVGWPCGALGPGPHRWGPCGGSWAQKFYQDGPGRGLGEEDIRRAREARPRKT
PRPQLSDRSRERKVPASRISRLANFGGLAVGLGLGVLAEMAKKSMPGGRLQSEGGSGLDSSPFLSEANAE
RIVQTLCTVRGAALKVGQMLSIQDNSFISPQLQHIFERVRQSADFMPRWQMLRVLEEELGRDWQAKVASL
EEVPFAAASIGQVHQGLLRDGTEVAVKIQYPGIAQSIQSDVQNLLAVLKMSAALPAGLFAEQSLQALQQE
LAWECDYRREAACAQNFRQLLANDPFFRVPAVVKELCTTRVLGMELAGGVPLDQCQGLSQDLRNQICFQL
LTLCLRELFEFRFMQTDPNWANFLYDASSHQVTLLDFGASREFGTEFTDHYIEVVKAAADGDRDCVLQKS
RDLKFLTGFETKAFSDAHVEAVMILGEPFATQGPYDFGSGETARRIQDLIPVLLRHRLCPPPEETYALHR
KLAGAFLACAHLRAHIACRDLFQDTYHRYWASRQPDAATAGSLPTKGDSWVDPS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_024876
ORF Size 1632 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_024876.2, NP_079152.3
RefSeq Size 2458 bp
RefSeq ORF 1635 bp
Locus ID 79934
UniProt ID Q96D53
Domains ABC1
Protein Families Druggable Genome, Protein Kinase
MW 60.1 kDa
Gene Summary This gene encodes a protein with two copies of a domain found in protein kinases. The encoded protein has a complete protein kinase catalytic domain, and a truncated domain that contains only the active and binding sites of the protein kinase domain, however, it is not known whether the protein has any kinase activity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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