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TOE1 (NM_025077) Human Tagged ORF Clone
CAT＃: RC203377
TOE1 (Myc-DDK-tagged)-Human target of EGR1, member 1 (nuclear) (TOE1)
ORF Plasmid: tGFP
"NM_025077" in other vectors (6)
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CNY 3,800.00
CNY 4,560.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | hCaf1z; PCH7 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC203377 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGCCGACAGTGACGATGGCGCAGTTTCAGCTCCCGCAGCTTCCGACGGTGGTGTCAGCAAAAGCA CAACATCTGGGGAGGAGCTAGTAGTCCAGGTTCCCGTAGTGGATGTGCAAAGCAACAACTTCAAGGAGAT GTGGCCATCCCTCCTGCTAGCCATAAAGACAGCTAATTTCGTGGCTGTGGACACGGAGCTGAGTGGGCTT GGGGACAGGAAGAGTTTGCTGAACCAGTGCATTGAGGAACGTTACAAGGCCGTGTGTCATGCTGCCAGGA CCCGTTCTATCCTTTCCCTGGGCCTCGCCTGCTTCAAGCGGCAGCCAGACAAGGGTGAACATTCCTATCT GGCTCAAGTGTTCAATCTCACTCTGCTGTGCATGGAGGAGTATGTCATAGAACCAAAGTCTGTGCAGTTC CTGATACAGCATGGCTTCAACTTCAACCAGCAGTATGCCCAAGGCATCCCCTACCATAAGGGCAATGACA AGGGTGATGAGAGCCAGAGCCAGTCAGTACGGACCCTATTCCTGGAGCTAATCCGAGCCCGCCGGCCCCT GGTGCTACACAATGGCCTTATAGACTTGGTGTTCCTGTACCAGAACTTCTATGCACACCTCCCTGAGAGT CTGGGAACCTTCACCGCTGACCTGTGTGAGATGTTCCCAGCAGGCATTTATGACACCAAATATGCTGCTG AGTTTCATGCCCGTTTCGTGGCCTCCTACTTAGAATATGCCTTCCGGAAATGTGAACGGGAAAATGGGAA GCAGCGGGCAGCTGGCAGCCCACACCTTACCCTGGAGTTCTGCAACTATCCTTCCAGCATGAGGGACCAT ATTGATTACCGCTGCTGCCTGCCCCCAGCAACCCACCGTCCTCATCCCACCAGCATCTGTGACAACTTCT CGGCTTATGGCTGGTGCCCCCTGGGACCACAGTGTCCTCAGTCTCACGATATTGACCTTATCATTGACAC TGATGAGGCTGCGGCAGAGGACAAGCGGCGACGGCGACGACGTAGGGAAAAACGGAAGAGGGCTTTATTG AACCTACCGGGGACACAGACCTCTGGGGAAGCTAAGGATGGTCCTCCCAAGAAGCAGGTCTGTGGGGATA GCATCAAGCCTGAAGAAACCGAGCAGGAGGTGGCTGCCGATGAAACTAGGAACCTGCCTCACTCCAAGCA AGGCAACAAAAATGACTTAGAGATGGGGATTAAGGCAGCAAGGCCTGAAATAGCTGATAGAGCTACCTCA GAAGTGCCAGGGAGCCAAGCCAGTCCTAACCCAGTGCCTGGGGATGGATTGCACCGGGCTGGTTTTGATG CCTTTATGACAGGTTATGTGATGGCCTATGTGGAAGTGAGCCAGGGACCGCAGCCCTGCAGCTCTGGACC CTGGCTCCCTGAATGCCACAATAAGGTATATTTGAGTGGCAAAGCTGTACCCCTCACAGTGGCCAAGAGC CAGTTCTCTCGTTCCTCCAAAGCCCACAATCAGAAGATGAAGCTCACTTGGGGCAGTAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203377 protein sequence
Red=Cloning site Green=Tags(s) MAADSDDGAVSAPAASDGGVSKSTTSGEELVVQVPVVDVQSNNFKEMWPSLLLAIKTANFVAVDTELSGL GDRKSLLNQCIEERYKAVCHAARTRSILSLGLACFKRQPDKGEHSYLAQVFNLTLLCMEEYVIEPKSVQF LIQHGFNFNQQYAQGIPYHKGNDKGDESQSQSVRTLFLELIRARRPLVLHNGLIDLVFLYQNFYAHLPES LGTFTADLCEMFPAGIYDTKYAAEFHARFVASYLEYAFRKCERENGKQRAAGSPHLTLEFCNYPSSMRDH IDYRCCLPPATHRPHPTSICDNFSAYGWCPLGPQCPQSHDIDLIIDTDEAAAEDKRRRRRRREKRKRALL NLPGTQTSGEAKDGPPKKQVCGDSIKPEETEQEVAADETRNLPHSKQGNKNDLEMGIKAARPEIADRATS EVPGSQASPNPVPGDGLHRAGFDAFMTGYVMAYVEVSQGPQPCSSGPWLPECHNKVYLSGKAVPLTVAKS QFSRSSKAHNQKMKLTWGSS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_025077 |
| ORF Size | 1530 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_025077.4 |
| RefSeq Size | 2408 bp |
| RefSeq ORF | 1533 bp |
| Locus ID | 114034 |
| UniProt ID | Q96GM8 |
| Protein Families | Stem cell - Pluripotency, Transcription Factors |
| MW | 56.5 kDa |
| Gene Summary | Inhibits cell growth rate and cell cycle. Induces CDKN1A expression as well as TGF-beta expression. Mediates the inhibitory growth effect of EGR1. Involved in the maturation of snRNAs and snRNA 3'-tail processing (PubMed:28092684).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203377L1 | Lenti ORF clone of Human target of EGR1, member 1 (nuclear) (TOE1), Myc-DDK-tagged |
CNY 6,200.00 |
|
| RC203377L2 | Lenti ORF clone of Human target of EGR1, member 1 (nuclear) (TOE1), mGFP tagged |
CNY 6,460.00 |
|
| RC203377L3 | Lenti ORF clone of Human target of EGR1, member 1 (nuclear) (TOE1), Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC203377L4 | Lenti ORF clone of Human target of EGR1, member 1 (nuclear) (TOE1), mGFP tagged |
CNY 6,460.00 |
|
| RG203377 | TOE1 (tGFP-tagged) - Human target of EGR1, member 1 (nuclear) (TOE1) |
CNY 5,400.00 |
|
| SC122981 | TOE1 (untagged)-Human target of EGR1, member 1 (nuclear) (TOE1) |
CNY 3,808.00 |
