NSDHL (NM_015922) Human Tagged ORF Clone
CAT#: RC203225
- TrueORF®
NSDHL (Myc-DDK-tagged)-Human NAD(P) dependent steroid dehydrogenase-like (NSDHL), transcript variant 1
ORF Plasmid: tGFP
"NM_015922" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | H105E3; SDR31E1; XAP104 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC203225 representing NM_015922
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAACCAGCAGTTAGCGAGCCAATGAGAGACCAAGTCGCACGGACTCATTTGACAGAGGACACTCCCA AAGTGAATGCTGACATAGAAAAGGTTAACCAGAATCAGGCCAAGAGATGCACAGTGATCGGTGGCTCTGG ATTCCTGGGGCAGCACATGGTGGAGCAGTTGCTGGCAAGAGGATATGCTGTCAATGTATTTGATATCCAG CAAGGGTTTGATAATCCCCAGGTGCGGTTCTTTCTGGGTGACCTCTGCAGCCGACAGGATCTGTACCCAG CTCTGAAAGGTGTAAACACAGTTTTCCACTGTGCGTCACCCCCACCATCCAGTAACAACAAGGAGCTCTT TTATAGAGTGAATTACATTGGCACCAAGAATGTCATTGAAACTTGCAAAGAGGCTGGGGTTCAGAAACTC ATTTTAACCAGCAGTGCCAGTGTCATCTTTGAGGGCGTCGATATCAAGAATGGAACTGAAGACCTTCCCT ATGCCATGAAACCCATTGACTACTACACAGAGACTAAGATCTTACAGGAGAGGGCAGTTCTGGGCGCCAA CGATCCTGAGAAGAATTTCTTAACCACAGCCATCCGCCCTCATGGCATTTTCGGCCCAAGGGACCCGCAG TTGGTACCCATCCTCATCGAGGCAGCCAGGAACGGCAAGATGAAGTTCGTGATTGGAAATGGGAAGAACT TGGTGGACTTCACCTTTGTGGAGAACGTGGTCCATGGACACATCCTGGCGGCAGAGCAGCTCTCCCGAGA CTCGACACTGGGTGGGAAGGCATTTCACATCACCAATGATGAGCCCATCCCTTTCTGGACATTCCTGTCT CGCATCCTGACAGGCCTCAATTATGAGGCCCCCAAGTACCACATCCCCTACTGGGTGGCCTACTACCTGG CCCTCCTGCTATCCCTGCTGGTGATGGTGATCAGTCCTGTCATCCAGCTGCAGCCCACCTTCACACCCAT GCGGGTCGCACTGGCTGGCACATTCCACTACTACAGCTGCGAGAGAGCCAAAAAGGCCATGGGCTACCAG CCACTAGTGACCATGGATGATGCTATGGAGAGGACCGTGCAGAGCTTTCGCCACCTGCGGAGGGTCAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203225 representing NM_015922
Red=Cloning site Green=Tags(s) MEPAVSEPMRDQVARTHLTEDTPKVNADIEKVNQNQAKRCTVIGGSGFLGQHMVEQLLARGYAVNVFDIQ QGFDNPQVRFFLGDLCSRQDLYPALKGVNTVFHCASPPPSSNNKELFYRVNYIGTKNVIETCKEAGVQKL ILTSSASVIFEGVDIKNGTEDLPYAMKPIDYYTETKILQERAVLGANDPEKNFLTTAIRPHGIFGPRDPQ LVPILIEAARNGKMKFVIGNGKNLVDFTFVENVVHGHILAAEQLSRDSTLGGKAFHITNDEPIPFWTFLS RILTGLNYEAPKYHIPYWVAYYLALLLSLLVMVISPVIQLQPTFTPMRVALAGTFHYYSCERAKKAMGYQ PLVTMDDAMERTVQSFRHLRRVK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_015922 |
ORF Size | 1119 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_015922.3 |
RefSeq Size | 1581 bp |
RefSeq ORF | 1122 bp |
Locus ID | 50814 |
UniProt ID | Q15738 |
Domains | 3Beta_HSD |
Protein Families | Transmembrane |
Protein Pathways | Metabolic pathways, Steroid biosynthesis |
MW | 41.9 kDa |
Gene Summary | The protein encoded by this gene is localized in the endoplasmic reticulum and is involved in cholesterol biosynthesis. Mutations in this gene are associated with CHILD syndrome, which is a X-linked dominant disorder of lipid metabolism with disturbed cholesterol biosynthesis, and typically lethal in males. Alternatively spliced transcript variants with differing 5' UTR have been found for this gene. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC203225L3 | Lenti-ORF clone of NSDHL (Myc-DDK-tagged)-Human NAD(P) dependent steroid dehydrogenase-like (NSDHL), transcript variant 1 |
CNY 5,890.00 |
|
RC203225L4 | Lenti-ORF clone of NSDHL (mGFP-tagged)-Human NAD(P) dependent steroid dehydrogenase-like (NSDHL), transcript variant 1 |
CNY 6,056.00 |
|
RG203225 | NSDHL (tGFP-tagged) - Human NAD(P) dependent steroid dehydrogenase-like (NSDHL), transcript variant 1 |
CNY 4,370.00 |
|
SC114562 | NSDHL (untagged)-Human NAD(P) dependent steroid dehydrogenase-like (NSDHL), transcript variant 1 |
CNY 3,656.00 |