Glutathione Synthetase (GSS) (NM_000178) Human Tagged ORF Clone
CAT#: RC203174
GSS (Myc-DDK-tagged)-Human glutathione synthetase (GSS)
ORF Plasmid: tGFP
"NM_000178" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | GSHS; HEL-S-64p; HEL-S-88n |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC203174 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCACCAACTGGGGGAGCCTCTTGCAGGATAAACAGCAGCTAGAGGAGCTGGCACGGCAGGCCGTGG ACCGGGCCCTGGCTGAGGGAGTATTGCTGAGGACCTCACAGGAGCCCACTTCCTCGGAGGTGGTGAGCTA TGCCCCATTCACGCTCTTCCCCTCACTGGTCCCCAGTGCCCTGCTGGAGCAAGCCTATGCTGTGCAGATG GACTTCAACCTGCTAGTGGATGCTGTCAGCCAGAACGCTGCCTTCCTGGAGCAAACTCTTTCCAGCACCA TCAAACAGGATGACTTTACCGCTCGTCTCTTTGACATCCACAAGCAAGTCCTAAAAGAGGGCATTGCCCA GACTGTGTTCCTGGGCCTGAATCGCTCAGACTACATGTTCCAGCGCAGCGCAGATGGCTCCCCAGCCCTG AAACAGATCGAAATCAACACCATCTCTGCCAGCTTTGGGGGCCTGGCCTCCCGGACCCCAGCTGTGCACC GACATGTTCTCAGTGTCCTGAGTAAGACCAAAGAAGCTGGCAAGATCCTCTCTAATAATCCCAGCAAGGG ACTGGCCCTGGGAATTGCCAAAGCCTGGGAGCTCTACGGCTCACCCAATGCTCTGGTGCTACTGATTGCT CAAGAGAAGGAAAGAAACATATTTGACCAGCGTGCCATAGAGAATGAGCTACTGGCCAGGAACATCCATG TGATCCGACGAACATTTGAAGATATCTCTGAAAAGGGGTCTCTGGACCAAGACCGAAGGCTGTTTGTGGA TGGCCAGGAAATTGCTGTGGTTTACTTCCGGGATGGCTACATGCCTCGTCAGTACAGTCTACAGAATTGG GAAGCACGTCTACTGCTGGAGAGGTCACATGCTGCCAAGTGCCCAGACATTGCCACCCAGCTGGCTGGGA CTAAGAAGGTGCAGCAGGAGCTAAGCAGGCCGGGCATGCTGGAGATGTTGCTCCCTGGCCAGCCTGAGGC TGTGGCCCGCCTCCGCGCCACCTTTGCTGGCCTCTACTCACTGGATGTGGGTGAAGAAGGGGACCAGGCC ATCGCCGAGGCCCTTGCTGCCCCTAGCCGGTTTGTGCTAAAGCCCCAGAGAGAGGGTGGAGGTAACAACC TATATGGGGAGGAAATGGTACAGGCCCTGAAACAGCTGAAGGACAGTGAGGAGAGGGCCTCCTACATCCT CATGGAGAAGATCGAACCTGAGCCTTTTGAGAATTGCCTGCTACGGCCTGGCAGCCCTGCCCGAGTGGTC CAGTGCATTTCAGAGCTGGGCATCTTTGGGGTCTATGTCAGGCAGGAAAAGACACTCGTGATGAACAAGC ACGTGGGGCATCTACTTCGAACCAAAGCCATCGAGCATGCAGATGGTGGTGTGGCAGCGGGAGTGGCAGT CCTGGACAACCCATACCCTGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203174 protein sequence
Red=Cloning site Green=Tags(s) MATNWGSLLQDKQQLEELARQAVDRALAEGVLLRTSQEPTSSEVVSYAPFTLFPSLVPSALLEQAYAVQM DFNLLVDAVSQNAAFLEQTLSSTIKQDDFTARLFDIHKQVLKEGIAQTVFLGLNRSDYMFQRSADGSPAL KQIEINTISASFGGLASRTPAVHRHVLSVLSKTKEAGKILSNNPSKGLALGIAKAWELYGSPNALVLLIA QEKERNIFDQRAIENELLARNIHVIRRTFEDISEKGSLDQDRRLFVDGQEIAVVYFRDGYMPRQYSLQNW EARLLLERSHAAKCPDIATQLAGTKKVQQELSRPGMLEMLLPGQPEAVARLRATFAGLYSLDVGEEGDQA IAEALAAPSRFVLKPQREGGGNNLYGEEMVQALKQLKDSEERASYILMEKIEPEPFENCLLRPGSPARVV QCISELGIFGVYVRQEKTLVMNKHVGHLLRTKAIEHADGGVAAGVAVLDNPYPV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_000178 |
ORF Size | 1422 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_000178.4 |
RefSeq Size | 1918 bp |
RefSeq ORF | 1425 bp |
Locus ID | 2937 |
UniProt ID | P48637 |
Domains | GSH_synthase, GSH_synth_ATP |
Protein Families | Druggable Genome |
Protein Pathways | Glutathione metabolism, Metabolic pathways |
MW | 52.4 kDa |
Gene Summary | Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. The protein encoded by this gene functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. Defects in this gene are a cause of glutathione synthetase deficiency. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC203174L1 | Lenti ORF clone of Human glutathione synthetase (GSS), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC203174L2 | Lenti ORF clone of Human glutathione synthetase (GSS), mGFP tagged |
CNY 6,056.00 |
|
RC203174L3 | Lenti ORF clone of Human glutathione synthetase (GSS), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC203174L4 | Lenti ORF clone of Human glutathione synthetase (GSS), mGFP tagged |
CNY 5,890.00 |
|
RG203174 | GSS (tGFP-tagged) - Human glutathione synthetase (GSS) |
CNY 5,256.00 |
|
SC319136 | GSS (untagged)-Human glutathione synthetase (GSS) |
CNY 3,656.00 |
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