Methionyl Aminopeptidase 1 (METAP1) (NM_015143) Human Tagged ORF Clone
CAT#: RC203022
METAP1 (Myc-DDK-tagged)-Human methionyl aminopeptidase 1 (METAP1)
ORF Plasmid: tGFP
"NM_015143" in other vectors (7)
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CNY 2,400.00
CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | MAP1A; MetAP1A |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC203022 representing NM_015143
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGAATCTGAACAGGCTCTTAAAGGTACTTCTCAGATTAAATTACTCTCATCTGAAGATATAGAAG GGATGCGACTTGTATGTAGGCTTGCTAGAGAAGTTTTGGATGTTGCTGCCGGCATGATTAAACCAGGTGT AACTACTGAAGAAATAGATCACGCTGTACACTTAGCATGTATTGCAAGAAATTGCTACCCTTCTCCCCTG AATTATTATAATTTCCCAAAGTCTTGTTGTACCTCAGTGAATGAAGTCATTTGCCATGGAATACCAGACA GAAGGCCCTTACAAGAAGGTGACATTGTTAATGTGGATATCACTCTTTATCGCAATGGTTATCATGGGGA CCTGAATGAGACATTTTTTGTTGGAGAAGTGGATGATGGAGCACGGAAACTTGTTCAGACCACATATGAG TGCCTGATGCAAGCCATTGATGCAGTGAAGCCTGGTGTTCGGTACAGAGAATTGGGAAACATTATCCAGA AGCATGCCCAAGCAAATGGGTTTTCAGTTGTTCGAAGCTATTGTGGGCATGGAATCCACAAGCTTTTTCA TACAGCTCCCAATGTACCCCACTATGCTAAAAATAAAGCAGTTGGAGTGATGAAGTCGGGCCATGTATTT ACAATTGAGCCAATGATTTGTGAAGGCGGATGGCAGGATGAAACCTGGCCAGATGGTTGGACTGCGGTGA CAAGAGACGGAAAGCGGTCTGCTCAGTTTGAGCACACCCTCCTGGTCACAGACACTGGCTGTGAAATCCT AACCCGGCGACTTGACAGTGCACGGCCTCACTTCATGTCTCAATTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203022 representing NM_015143
Red=Cloning site Green=Tags(s) MSESEQALKGTSQIKLLSSEDIEGMRLVCRLAREVLDVAAGMIKPGVTTEEIDHAVHLACIARNCYPSPL NYYNFPKSCCTSVNEVICHGIPDRRPLQEGDIVNVDITLYRNGYHGDLNETFFVGEVDDGARKLVQTTYE CLMQAIDAVKPGVRYRELGNIIQKHAQANGFSVVRSYCGHGIHKLFHTAPNVPHYAKNKAVGVMKSGHVF TIEPMICEGGWQDETWPDGWTAVTRDGKRSAQFEHTLLVTDTGCEILTRRLDSARPHFMSQF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_015143 |
ORF Size | 816 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_015143.1, NP_055958.1 |
RefSeq Size | 2835 bp |
RefSeq ORF | 1161 bp |
Locus ID | 23173 |
UniProt ID | P53582 |
Domains | Peptidase_M24 |
Protein Families | Druggable Genome |
MW | 30.37 kDa |
Gene Summary | Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Required for normal progression through the cell cycle.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC203022L1 | Lenti ORF clone of Human methionyl aminopeptidase 1 (METAP1), Myc-DDK-tagged |
CNY 4,800.00 |
|
RC203022L2 | Lenti ORF clone of Human methionyl aminopeptidase 1 (METAP1), mGFP tagged |
CNY 5,890.00 |
|
RC203022L3 | Lenti ORF clone of Human methionyl aminopeptidase 1 (METAP1), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC203022L4 | Lenti ORF clone of Human methionyl aminopeptidase 1 (METAP1), mGFP tagged |
CNY 5,890.00 |
|
RG203022 | METAP1 (tGFP-tagged) - Human methionyl aminopeptidase 1 (METAP1) |
CNY 4,000.00 |
|
SC100433 | METAP1 (untagged)-Human methionyl aminopeptidase 1 (METAP1) |
CNY 6,920.00 |
|
SC317619 | METAP1 (untagged)-Human methionyl aminopeptidase 1 (METAP1) |
CNY 6,270.00 |
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