PRIM1 (NM_000946) Human Tagged ORF Clone
CAT#: RC202710
PRIM1 (Myc-DDK-tagged)-Human primase, DNA, polypeptide 1 (49kDa) (PRIM1)
ORF Plasmid: tGFP
"NM_000946" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 3,656.00
CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | p49 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC202710 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGACGTTTGACCCCACCGAGCTGCCCGAGCTGCTTAAACTTTATTACCGGAGGCTCTTTCCCTACT CTCAGTACTATCGCTGGCTCAACTACGGTGGAGTGATAAAGAATTACTTTCAACACCGTGAATTTTCATT CACATTGAAAGATGATATTTACATTCGCTACCAATCCTTCAACAACCAGAGTGATCTGGAAAAGGAGATG CAGAAAATGAATCCATACAAGATTGATATAGGCGCAGTATATTCTCACAGACCCAATCAACACAATACAG TGAAGCTGGGAGCTTTCCAGGCTCAGGAAAAAGAACTGGTATTTGACATTGACATGACAGACTATGACGA TGTGAGGAGATGTTGTAGTTCTGCAGACATATGTCCTAAGTGCTGGACCCTCATGACAATGGCCATACGC ATCATTGACAGAGCATTGAAGGAGGACTTTGGATTTAAGCATCGTCTCTGGGTATATTCTGGAAGGAGAG GTGTTCATTGTTGGGTCTGTGATGAATCAGTTAGAAAACTGTCTTCTGCAGTACGTTCTGGGATAGTTGA GTATTTGAGCCTTGTAAAGGGTGGTCAAGACGTTAAAAAGAAAGTTCACCTAAGTGAAAAAATTCACCCT TTTATCAGAAAATCTATAAACATAATAAAAAAATACTTTGAAGAATATGCCTTGGTTAATCAAGATATTC TCGAAAATAAAGAAAGCTGGGATAAGATTTTAGCCCTTGTTCCTGAAACAATTCATGATGAACTTCAACA AAGCTTCCAAAAGTCTCACAATTCACTTCAGCGTTGGGAGCACTTGAAGAAAGTAGCCAGCAGATATCAG AATAACATCAAAAATGACAAATATGGACCCTGGCTGGAGTGGGAGATTATGCTCCAGTACTGTTTTCCAC GGCTGGATATCAATGTCAGCAAAGGAATCAATCATCTACTGAAGAGCCCTTTTAGTGTTCATCCTAAAAC AGGTCGCATATCTGTGCCTATTGATTTGCAGAAAGTGGACCAGTTTGATCCATTTACTGTTCCGACCATA AGCTTCATCTGCCGTGAATTGGATGCCATTTCCACTAATGAAGAGGAAAAAGAGGAGAATGAAGCTGAAT CTGATGTCAAACATAGAACCAGAGATTATAAGAAGACCAGTCTAGCACCTTATGTGAAAGTTTTTGAACA TTTTCTTGAAAATCTGGATAAATCCCGAAAAGGAGAACTTCTTAAGAAGAGTGATTTACAAAAAGATTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202710 protein sequence
Red=Cloning site Green=Tags(s) METFDPTELPELLKLYYRRLFPYSQYYRWLNYGGVIKNYFQHREFSFTLKDDIYIRYQSFNNQSDLEKEM QKMNPYKIDIGAVYSHRPNQHNTVKLGAFQAQEKELVFDIDMTDYDDVRRCCSSADICPKCWTLMTMAIR IIDRALKEDFGFKHRLWVYSGRRGVHCWVCDESVRKLSSAVRSGIVEYLSLVKGGQDVKKKVHLSEKIHP FIRKSINIIKKYFEEYALVNQDILENKESWDKILALVPETIHDELQQSFQKSHNSLQRWEHLKKVASRYQ NNIKNDKYGPWLEWEIMLQYCFPRLDINVSKGINHLLKSPFSVHPKTGRISVPIDLQKVDQFDPFTVPTI SFICRELDAISTNEEEKEENEAESDVKHRTRDYKKTSLAPYVKVFEHFLENLDKSRKGELLKKSDLQKDF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_000946 |
ORF Size | 1260 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_000946.3 |
RefSeq Size | 1471 bp |
RefSeq ORF | 1263 bp |
Locus ID | 5557 |
UniProt ID | P49642 |
Protein Families | Druggable Genome |
Protein Pathways | DNA replication, Metabolic pathways, Purine metabolism, Pyrimidine metabolism |
MW | 49.9 kDa |
Gene Summary | The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. The protein encoded by this gene is the small, 49 kDa primase subunit. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC202710L3 | Lenti ORF clone of Human primase, DNA, polypeptide 1 (49kDa) (PRIM1), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC202710L4 | Lenti ORF clone of Human primase, DNA, polypeptide 1 (49kDa) (PRIM1), mGFP tagged |
CNY 5,890.00 |
|
RG202710 | PRIM1 (tGFP-tagged) - Human primase, DNA, polypeptide 1 (49kDa) (PRIM1) |
CNY 5,256.00 |
|
SC126303 | PRIM1 (untagged)-Human primase, DNA, polypeptide 1 (49kDa) (PRIM1) |
CNY 3,656.00 |