United States
Germany
Japan
United Kingdom
China
BRAF35 (HMG20B) (NM_006339) Human Tagged ORF Clone
CAT＃: RC202444
HMG20B (Myc-DDK-tagged)-Human high mobility group 20B (HMG20B)
ORF Plasmid: tGFP
"NM_006339" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 2,400.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BRAF25; BRAF35; HMGX2; HMGXB2; PP7706; pp8857; SMARCE1r; SOXL |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC202444 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCCACGGCCCCAAGCAGCCCGGCGCGGCCGCCGCGCCGGCGGGCGGCAAGGCTCCGGGCCAGCATG GGGGCTTCGTGGTGACTGTCAAGCAAGAGCGCGGCGAGGGTCCACGCGCGGGCGAGAAGGGGTCCCACGA GGAGGAGCCGGTGAAGAAACGCGGCTGGCCCAAGGGCAAGAAGCGGAAGAAGATTCTGCCGAATGGGCCC AAGGCACCGGTCACGGGCTACGTGCGCTTCCTGAACGAGCGGCGCGAGCAGATCCGCACGCGCCACCCGG ATCTGCCCTTTCCCGAGATCACCAAGATGCTGGGCGCCGAGTGGAGCAAGCTGCAGCCAACGGAAAAGCA GCGGTACCTGGATGAGGCCGAGAGAGAGAAGCAGCAGTACATGAAGGAGCTGCGGGCGTACCAGCAGTCT GAAGCCTATAAGATGTGCACGGAGAAGATCCAGGAGAAGAAGATCAAGAAAGAAGACTCGAGCTCTGGGC TCATGAACACTCTCCTGAATGGACACAAGGGTGGGGACTGCGATGGCTTCTCCACCTTCGATGTTCCCAT CTTCACTGAAGAGTTCTTGGACCAAAACAAAGCGCGTGAGGCGGAGCTTCGGCGCTTGCGGAAGATGAAT GTGGCCTTCGAGGAGCAGAACGCGGTACTGCAGAGGCACACGCAGAGCATGAGCAGCGCGCGCGAGCGTC TGGAGCAGGAGCTGGCGCTGGAGGAGCGGAGGACGCTGGCGCTGCAGCAGCAGCTCCAGGCCGTGCGCCA GGCGCTCACCGCCAGCTTCGCCTCACTGCCGGTGCCGGGCACGGGCGAAACGCCCACGCTGGGCACTCTG GACTTCTACATGGCCCGGCTTCACGGAGCCATCGAGCGCGACCCCGCCCAGCACGAGAAGCTCATCGTCC GCATCAAGGAAATCCTGGCCCAGGTCGCCAGCGAGCACCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202444 protein sequence
Red=Cloning site Green=Tags(s) MSHGPKQPGAAAAPAGGKAPGQHGGFVVTVKQERGEGPRAGEKGSHEEEPVKKRGWPKGKKRKKILPNGP KAPVTGYVRFLNERREQIRTRHPDLPFPEITKMLGAEWSKLQPTEKQRYLDEAEREKQQYMKELRAYQQS EAYKMCTEKIQEKKIKKEDSSSGLMNTLLNGHKGGDCDGFSTFDVPIFTEEFLDQNKAREAELRRLRKMN VAFEEQNAVLQRHTQSMSSARERLEQELALEERRTLALQQQLQAVRQALTASFASLPVPGTGETPTLGTL DFYMARLHGAIERDPAQHEKLIVRIKEILAQVASEHL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006339 |
| ORF Size | 951 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006339.3 |
| RefSeq Size | 1584 bp |
| RefSeq ORF | 954 bp |
| Locus ID | 10362 |
| UniProt ID | Q9P0W2 |
| Protein Families | Druggable Genome, Transcription Factors |
| MW | 35.8 kDa |
| Gene Summary | Required for correct progression through G2 phase of the cell cycle and entry into mitosis. Required for RCOR1/CoREST mediated repression of neuronal specific gene promoters.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202444L1 | Lenti ORF clone of Human high mobility group 20B (HMG20B), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC202444L2 | Lenti ORF clone of Human high mobility group 20B (HMG20B), mGFP tagged |
CNY 5,890.00 |
|
| RC202444L3 | Lenti ORF clone of Human high mobility group 20B (HMG20B), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202444L4 | Lenti ORF clone of Human high mobility group 20B (HMG20B), mGFP tagged |
CNY 5,890.00 |
|
| RG202444 | HMG20B (tGFP-tagged) - Human high mobility group 20B (HMG20B) |
CNY 4,000.00 |
|
| SC127219 | HMG20B (untagged)-Human high mobility group 20B (HMG20B) |
CNY 3,656.00 |
