AFG1L (NM_145315) Human Tagged ORF Clone
CAT#: RC202313
LACE1 (Myc-DDK-tagged)-Human lactation elevated 1 (LACE1)
ORF Plasmid: tGFP
"NM_145315" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
Cited in 2 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | AFG1; c222389; LACE1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC202313 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCCTCCTGGTCGCTCTTGGTTACCCTGCGCCCCTTAGCACAGAGCCCGCTGAGAGGGAGATGTG TTGGGTGCGGGGCCTGGGCCGCCGCTCTCGCTCCTCTGGCCACCGCCCCTGGGAAGCCCTTTTGGAAAGC CTATACGGTTCAGACATCCGAGAGCATGACCCCAACTGCCACTTCAGAGACTTATTTGAAAGCTTTGGCC GTTTGCCATGGACCTCTGGACCACTATGATTTTCTGATCAAAGCTCATGAGCTAAAGGATGATGAACATC AAAGAAGAGTCATACAGTGTTTGCAGAAATTACACGAGGACCTTAAAGGATACAATATAGAGGCAGAAGG CCTTTTTTCAAAGCTTTTTTCAAGGAGCAAACCTCCAAGGGGCCTGTATGTTTATGGAGATGTTGGTACA GGAAAAACAATGGTGATGGACATGTTTTATGCTTATGTGGAAATGAAGAGGAAAAAACGGGTTCATTTTC ATGGTTTCATGCTAGATGTGCACAAAAGAATACATCGCCTTAAACAGAGTTTGCCAAAAAGGAAACCAGG ATTCATGGCTAAATCATATGACCCAATAGCTCCCATAGCCGAAGAAATCAGCGAAGAAGCATGTCTCCTA TGTTTTGATGAATTTCAGGTCACTGACATTGCTGATGCCATGATTCTGAAACAGCTTTTTGAAAATCTGT TCAAAAACGGGGTCGTCGTTGTGGCAACATCCAACAGGCCACCGGAAGATCTCTATAAAAATGGACTCCA AAGAGCTAACTTTGTACCATTCATAGCAGTCTTGAAGGAATATTGTAATACAGTCCAGCTAGATTCTGGG ATAGATTACCGGAAAAGGGAACTTCCTGCTGCAGGAAAACTCTACTACCTCACAAGTGAAGCTGATGTGG AGGCTGTCATGGATAAGTTGTTTGATGAGCTGGCTCAGAAACAAAATGATTTAACTAGACCAAGGATTCT AAAAGTGCAAGGCAGAGAGCTGCGCCTGAATAAAGCCTGTGGAACCGTTGCCGACTGCACATTTGAAGAG CTGTGTGAGAGACCACTTGGAGCCAGTGACTATTTGGAACTATCAAAGAATTTTGATACAATATTTTTAC GAAACATTCCGCAATTTACTCTGGCAAACAGGACTCAAGGTCGAAGATTCATAACTCTCATCGATAACTT TTATGATCTCAAGGTGCGTATAATTTGCTCTGCGTCGACTCCTATATCAAGCTTATTTTTGCATCAACAT CATGACAGTGAGTTGGAGCAAAGCAGAATACTGATGGATGATTTGGGGCTGAGCCAGGATTCAGCAGAAG GACTCTCCATGTTTACCGGAGAAGAGGAAATCTTTGCATTTCAGCGCACAATTTCCCGACTCACGGAAAT GCAGACTGAACAGTACTGGAATGAAGGAGACAGAACCAAGAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202313 protein sequence
Red=Cloning site Green=Tags(s) MAASWSLLVTLRPLAQSPLRGRCVGCGAWAAALAPLATAPGKPFWKAYTVQTSESMTPTATSETYLKALA VCHGPLDHYDFLIKAHELKDDEHQRRVIQCLQKLHEDLKGYNIEAEGLFSKLFSRSKPPRGLYVYGDVGT GKTMVMDMFYAYVEMKRKKRVHFHGFMLDVHKRIHRLKQSLPKRKPGFMAKSYDPIAPIAEEISEEACLL CFDEFQVTDIADAMILKQLFENLFKNGVVVVATSNRPPEDLYKNGLQRANFVPFIAVLKEYCNTVQLDSG IDYRKRELPAAGKLYYLTSEADVEAVMDKLFDELAQKQNDLTRPRILKVQGRELRLNKACGTVADCTFEE LCERPLGASDYLELSKNFDTIFLRNIPQFTLANRTQGRRFITLIDNFYDLKVRIICSASTPISSLFLHQH HDSELEQSRILMDDLGLSQDSAEGLSMFTGEEEIFAFQRTISRLTEMQTEQYWNEGDRTKK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_145315 |
ORF Size | 1443 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_145315.5 |
RefSeq Size | 2262 bp |
RefSeq ORF | 1446 bp |
Locus ID | 246269 |
UniProt ID | Q8WV93 |
MW | 54.8 kDa |
Gene Summary | This gene encodes a mitochondrial integral membrane protein that plays a role in mitochondrial protein homeostasis. The protein contains a P-loop motif and a five-domain structure that is conserved in fly, yeast, and bacteria. It functions to mediate the degradation of nuclear-encoded complex IV subunits. Two conserved estrogen receptor binding sites are located within 2.5 kb of this gene. Polymorphisms in this gene have been associated with bipolar disorder. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2016] |
Citations (2)
The use of this cDNA Clones has been cited in the following citations: |
---|
LACE1 interacts with p53 and mediates its mitochondrial translocation and apoptosis
,Cesnekova, J;Spacilova, J;Hansikova, H;Houstek, J;Zeman, J;Stiburek, L;,
Oncotarget
,PubMed ID 27323408
[AFG1L]
|
The mammalian homologue of yeast AFG1 ATPase (Lactation elevated 1) mediates degradation of nuclear-encoded complex IV subunits
,Cesnekova, J;Rodinova, M;Hansikova, H;Houstek, J;Zeman, J;Stiburek, L;,
Biochem. J.
,PubMed ID 26759378
[AFG1L]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC202313L1 | Lenti ORF clone of Human lactation elevated 1 (LACE1), Myc-DDK-tagged |
CNY 6,056.00 |
|
RC202313L2 | Lenti ORF clone of Human lactation elevated 1 (LACE1), mGFP tagged |
CNY 5,890.00 |
|
RC202313L3 | Lenti ORF clone of Human lactation elevated 1 (LACE1), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC202313L4 | Lenti ORF clone of Human lactation elevated 1 (LACE1), mGFP tagged |
CNY 5,890.00 |
|
RG202313 | LACE1 (tGFP-tagged) - Human lactation elevated 1 (LACE1) |
CNY 5,256.00 |
|
SC123228 | LACE1 (untagged)-Human lactation elevated 1 (LACE1) |
CNY 3,656.00 |