RBMXL1 (NM_019610) Human Tagged ORF Clone

CAT#: RC202165

  • TrueORF®

RBMXL1 (Myc-DDK-tagged)-Human RNA binding motif protein, X-linked-like 1 (RBMXL1), transcript variant 2

ORF Plasmid: DDK tGFP



  "NM_019610" in other vectors (4)

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CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal Anti-RBMXL1 Antibody
    • 50 ug

CNY 4,628.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms RBM1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC202165 representing NM_019610
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTTGAAGCAGATCGCCCAGGAAAGCTCTTCATTGGTGGGCTTAATACGGAAACAAATGAGAAAGCTC
TTGAAACAGTATTTGGCAAATATGGACGAATAGTGGAAGTACTCTTGATAAAAGACCGTGAAACCAACAA
ATCAAGAGGATTTGCTTTTGTCACCTTTGAAAGCCCAGCAGACGCTAAGGATGCAGCCAGAGACATGAAT
GGAAAGTCATTAGATGGAAAAGCCATCAAGGTGGAACAAGCCACCAAACCATCATTTGAAAGAGGTAGAC
ATGGACCGCCCCCACCTCCAAGAAGTAGAGGTCCTCCAAGAGGTTTTGGAGCTGGAAGAGGAGGAAGTGG
AGGAACCAGGGGACCTCCTTCACGAGGAGGACACATGGATGATGGTGGATATTCCATGAATTTTAACATG
AGTTCTTCCAGGGGACCACTCCCAGTAAAAAGAGGACCACCACCAAGAAGTGGGGGTCCTTCTCCTAAGA
GATCTGCACCTTCAGGACTAGTTCGCAGCAGCAGTGGAATGGGAGGAAGAGCTCCTCTATCACGTGGAAG
AGATAGTTATGGAGGTCCACCTCGAAGGGAACCGCTCCCCTCTCGTAGAGATGTTTATTTGTCCCCAAGA
GATGATGGGTATTCTACTAAAGACAGCTATTCAAGCAGAGATTACCCAAGTTCTCGTGATACAAGAGATT
ATGCACCACCACCACGAGATTATACTTACCGTGATTATGGTCATTCCAGTTCACGTGATGACTATCCATC
AAGAGGCTATGGCGATAGAGATGGATATGGTCGTGATCGTGACTATTCAGATCATCCAAGTGGAGGTTCC
TACAGAGATTCATATGAGAGTTATGGTAACTCACGTAGTGCTCCACTTACACGAGGGCCCCCGCCATCTT
ATGGTGGAAGCAGTCGCTATGATGATTATAGCAGCTCACGTGATGGATATGGTGGAAGTCGAGACAGTTA
CTCAAGCAGCCGAAGTGATCTCTACTCAAGTTGTGACAGGGTTGGCAGACAAGAAAGAGGGCTTCCCCCT
TCTGTAGAAAGGGGGTACCCTTCTTCACGTGATTCCTACAGCAGTTCAAGCCGCGGAGCACCAAGAGGTG
CTGGCCCTGGAGGAAGCCGATCTGATAGAGGGGGAGGCAGAAGCAGATAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC202165 representing NM_019610
Red=Cloning site Green=Tags(s)

MVEADRPGKLFIGGLNTETNEKALETVFGKYGRIVEVLLIKDRETNKSRGFAFVTFESPADAKDAARDMN
GKSLDGKAIKVEQATKPSFERGRHGPPPPPRSRGPPRGFGAGRGGSGGTRGPPSRGGHMDDGGYSMNFNM
SSSRGPLPVKRGPPPRSGGPSPKRSAPSGLVRSSSGMGGRAPLSRGRDSYGGPPRREPLPSRRDVYLSPR
DDGYSTKDSYSSRDYPSSRDTRDYAPPPRDYTYRDYGHSSSRDDYPSRGYGDRDGYGRDRDYSDHPSGGS
YRDSYESYGNSRSAPLTRGPPPSYGGSSRYDDYSSSRDGYGGSRDSYSSSRSDLYSSCDRVGRQERGLPP
SVERGYPSSRDSYSSSSRGAPRGAGPGGSRSDRGGGRSRY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_019610
ORF Size 1170 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_019610.5, NP_062556.2
RefSeq Size 5028 bp
RefSeq ORF 1173 bp
Locus ID 494115
UniProt ID Q96E39
Domains RRM, RRM_1
MW 42.1 kDa
Gene Summary This gene represents a retrogene of RNA binding motif protein, X-linked (RBMX), which is located on chromosome X. While all introns in the coding sequence have been processed out compared to the RBMX locus, the ORF is intact and there is specific evidence for transcription at this location. The preservation of the ORF by purifying selection in all Old World monkeys carrying it suggests that this locus is likely to be functional, possibly during male meiosis when X chromosomal genes are silenced or during haploid stages of spermatogenesis. This gene shares 5' exon structure with the cysteine conjugate-beta lyase 2 locus on chromosome 1, but the coding sequences are non-overlapping. Alternative splicing results in two transcript variants. [provided by RefSeq, Jun 2009]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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