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MTGR1 (CBFA2T2) (NM_001039709) Human Tagged ORF Clone
CAT＃: RC202013
CBFA2T2 (Myc-DDK-tagged)-Human core-binding factor, runt domain, alpha subunit 2, translocated to, 2 (CBFA2T2), transcript variant 4
ORF Plasmid: tGFP
"NM_001039709" in other vectors (4)
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CNY 4,576.00
| Cited in 1 publication. |
Product images
CNY 300.00
CNY 6,281.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | EHT; MTGR1; p85; ZMYND3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC202013 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGTTTCACCATGTTGGCCAGGCTCGTCTTGAACTCCTGACCTCAGGTGATCTGCCTGCATTGGCCT CCCAACGTGCTGGGATTACAGTTGGTCCTGAGAAAAGGGTGCCAGCGATGCCTGGATCGCCTGTGGAAGT GAAGATACAGTCCAGATCCTCACCTCCCACCATGCCACCCCTCCCACCAATAAATCCTGGAGGACCGAGG CCAGTGTCCTTCACTCCTACTGCATTAAGCAATGGCATCAACCATTCTCCTCCTACCCTGAATGGTGCCC CATCACCGCCACAGAGATTCAGCAATGGTCCTGCCTCCTCCACATCATCTGCACTCACAAATCAGCAATT GCCAGCCACTTGTGGTGCTCGACAACTCAGCAAGTTGAAACGCTTTCTTACCACTCTGCAACAGTTTGGC AATGACATCTCCCCTGAGATTGGGGAGAAGGTGCGGACTCTTGTTCTTGCACTGGTGAACTCAACAGTGA CAATTGAGGAATTCCACTGTAAGCTCCAAGAAGCCACAAACTTTCCCCTTCGTCCTTTTGTGATTCCATT TCTCAAGGCCAACCTGCCCCTGCTGCAGCGGGAACTGCTGCACTGCGCTCGGGCGGCCAAGCAGACCCCA TCCCAGTACCTGGCTCAGCACGAACACCTTCTGCTCAACACAAGCATTGCATCGCCTGCTGACTCGTCAG AGTTGCTCATGGAGGTGCACGGAAATGGGAAGAGGCCCAGTCCAGAGAGGAGAGAAGAGAATAGTTTTGA TAGAGACACAATTGCTCCTGAGCCTCCTGCCAAGAGAGTATGTACCATCAGCCCTGCTCCTCGGCACAGT CCTGCTCTCACTGTGCCCCTCATGAATCCTGGGGGCCAATTCCATCCTACCCCTCCACCTCTTCAGCATT ACACCTTAGAGGATATTGCAACTTCTCACCTGTATCGGGAACCCAACAAGATGCTAGAGCATCGAGAAGT TCGTGATAGACACCACAGTCTTGGTCTAAATGGAGGCTATCAAGATGAGTTGGTAGATCATCGTTTGACA GAAAGGGAATGGGCTGATGAATGGAAACATCTTGACCATGCGCTGAATTGCATTATGGAAATGGTAGAGA AAACAAGGCGCTCTATGGCAGTTCTGCGGCGCTGTCAGGAATCAGATCGTGAAGAACTCAACTACTGGAA AAGACGGTACAATGAAAACACAGAGCTGAGGAAAACGGGGACCGAGTTGGTCTCCAGGCAGCACAGCCCT GGGAGTGCAGATTCTCTCAGCAATGATTCTCAGAGAGAGTTCAACAGCAGGCCAGGTACAGGATACGTAC CTGTGGAGTTTTGGAAAAAAACAGAAGAAGCTGTGAATAAGGTGAAAATTCAGGCCATGTCAGAAGTACA GAAGGCCGTCGCTGAGGCAGAGCAGAAAGCCTTTGAAGTGATTGCAACAGAGAGAGCACGAATGGAGCAA ACCATAGCGGATGTCAAGCGGCAGGCCGCAGAGGATGCTTTCCTCGTCATCAATGAGCAAGAGGAGTCCA CGGAGAACTGCTGGAACTGTGGCCGCAAAGCCAGCGAGACATGCAGTGGCTGCAATATCGCGCGATACTG TGGCTCTTTCTGCCAGCACAAGGACTGGGAGCGGCACCACCGCCTCTGTGGTCAGAACCTGCATGGCCAG AGCCCCCACGGCCAGGGCCGGCCGCTGCTTCCTGTAGGCAGGGGCTCCTCTGCCAGGTCCGCCGACTGCA GCGTGCCCAGCCCAGCCCTCGACAAGACCTCGGCAACCACATCGCGTTCCTCAACACCTGCTTCTGTGAC AGCTATCGACACCAACGGACTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202013 protein sequence
Red=Cloning site Green=Tags(s) MGFHHVGQARLELLTSGDLPALASQRAGITVGPEKRVPAMPGSPVEVKIQSRSSPPTMPPLPPINPGGPR PVSFTPTALSNGINHSPPTLNGAPSPPQRFSNGPASSTSSALTNQQLPATCGARQLSKLKRFLTTLQQFG NDISPEIGEKVRTLVLALVNSTVTIEEFHCKLQEATNFPLRPFVIPFLKANLPLLQRELLHCARAAKQTP SQYLAQHEHLLLNTSIASPADSSELLMEVHGNGKRPSPERREENSFDRDTIAPEPPAKRVCTISPAPRHS PALTVPLMNPGGQFHPTPPPLQHYTLEDIATSHLYREPNKMLEHREVRDRHHSLGLNGGYQDELVDHRLT EREWADEWKHLDHALNCIMEMVEKTRRSMAVLRRCQESDREELNYWKRRYNENTELRKTGTELVSRQHSP GSADSLSNDSQREFNSRPGTGYVPVEFWKKTEEAVNKVKIQAMSEVQKAVAEAEQKAFEVIATERARMEQ TIADVKRQAAEDAFLVINEQEESTENCWNCGRKASETCSGCNIARYCGSFCQHKDWERHHRLCGQNLHGQ SPHGQGRPLLPVGRGSSARSADCSVPSPALDKTSATTSRSSTPASVTAIDTNGL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001039709 |
| ORF Size | 1845 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq Size | 7449 bp |
| RefSeq ORF | 1728 bp |
| Locus ID | 9139 |
| UniProt ID | O43439 |
| Protein Families | Transcription Factors |
| MW | 68 kDa |
| Gene Summary | In acute myeloid leukemia, especially in the M2 subtype, the t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 (AML1) gene fused to the 3'-region of the CBFA2T1 (MTG8) gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. The protein encoded by this gene binds to the AML1-MTG8 complex and may be important in promoting leukemogenesis. Several transcript variants are thought to exist for this gene, but the full-length natures of only three have been described. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Application of immobilized ATP to the study of NLRP inflammasomes
,Liao, KC;Sandall, CF;Carlson, DA;Ulke-Lemée, A;Platnich, JM;Hughes, PF;Muruve, DA;Haystead, TAJ;MacDonald, JA;,
Arch. Biochem. Biophys.
,PubMed ID 30641048
[MTGR1]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202013L3 | Lenti ORF clone of Human core-binding factor, runt domain, alpha subunit 2; translocated to, 2 (CBFA2T2), transcript variant 4, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202013L4 | Lenti ORF clone of Human core-binding factor, runt domain, alpha subunit 2; translocated to, 2 (CBFA2T2), transcript variant 4, mGFP tagged |
CNY 5,890.00 |
|
| RG202013 | CBFA2T2 (tGFP-tagged) - Human core-binding factor, runt domain, alpha subunit 2; translocated to, 2 (CBFA2T2), transcript variant 4 |
CNY 6,176.00 |
|
| SC310836 | CBFA2T2 (untagged)-Human core-binding factor, runt domain, alpha subunit 2, translocated to, 2 (CBFA2T2), transcript variant 4 |
CNY 7,220.00 |
