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14 3 3 gamma (YWHAG) (NM_012479) Human Tagged ORF Clone
CAT＃: RC201925
YWHAG (Myc-DDK-tagged)-Human tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide (YWHAG)
ORF Plasmid: tGFP
"NM_012479" in other vectors (7)
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CNY 2,400.00
CNY 3,705.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 14-3-3GAMMA; DEE56; EIEE56; PPP1R170 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC201925 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGGACCGCGAGCAACTGGTGCAGAAAGCCCGGCTGGCCGAGCAGGCGGAGCGCTACGACGACATGG CCGCGGCCATGAAGAACGTGACAGAGCTGAATGAGCCACTGTCGAATGAGGAACGAAACCTTCTGTCTGT GGCCTACAAGAACGTTGTGGGGGCACGCCGCTCTTCCTGGAGGGTCATCAGTAGCATTGAGCAGAAGACA TCTGCAGACGGCAATGAGAAGAAGATTGAGATGGTCCGTGCGTACCGGGAGAAGATAGAGAAGGAGTTGG AGGCTGTGTGCCAGGATGTGCTGAGCCTGCTGGATAACTACCTGATCAAGAATTGCAGCGAGACCCAGTA CGAGAGCAAAGTGTTCTACCTGAAGATGAAAGGGGACTACTACCGCTACCTGGCTGAAGTGGCCACCGGA GAGAAAAGGGCGACGGTGGTGGAGTCCTCTGAGAAGGCCTACAGCGAAGCCCACGAGATCAGCAAAGAGC ACATGCAGCCCACCCACCCCATCCGATTAGGCCTGGCTCTTAACTACTCCGTCTTCTACTATGAGATCCA GAACGCCCCAGAGCAAGCGTGCCACTTGGCCAAGACCGCGTTCGACGACGCCATCGCCGAGCTTGACACC CTCAACGAGGACTCCTACAAGGACTCCACGCTCATCATGCAGCTCCTCCGCGACAACCTCACGCTCTGGA CGAGCGACCAGCAGGACGACGATGGCGGCGAAGGCAACAAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201925 protein sequence
Red=Cloning site Green=Tags(s) MVDREQLVQKARLAEQAERYDDMAAAMKNVTELNEPLSNEERNLLSVAYKNVVGARRSSWRVISSIEQKT SADGNEKKIEMVRAYREKIEKELEAVCQDVLSLLDNYLIKNCSETQYESKVFYLKMKGDYYRYLAEVATG EKRATVVESSEKAYSEAHEISKEHMQPTHPIRLGLALNYSVFYYEIQNAPEQACHLAKTAFDDAIAELDT LNEDSYKDSTLIMQLLRDNLTLWTSDQQDDDGGEGNN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012479 |
| ORF Size | 741 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_012479.4 |
| RefSeq Size | 3779 bp |
| RefSeq ORF | 744 bp |
| Locus ID | 7532 |
| UniProt ID | P61981 |
| Domains | 14-3-3 |
| Protein Families | Druggable Genome |
| Protein Pathways | Cell cycle, Neurotrophin signaling pathway, Oocyte meiosis |
| MW | 28.3 kDa |
| Gene Summary | This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201925L1 | Lenti ORF clone of Human tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide (YWHAG), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC201925L2 | Lenti ORF clone of Human tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide (YWHAG), mGFP tagged |
CNY 5,890.00 |
|
| RC201925L3 | Lenti ORF clone of Human tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide (YWHAG), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201925L4 | Lenti ORF clone of Human tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide (YWHAG), mGFP tagged |
CNY 4,800.00 |
|
| RG201925 | YWHAG (tGFP-tagged) - Human tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide (YWHAG) |
CNY 4,000.00 |
|
| SC124450 | YWHAG (untagged)-Human tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide (YWHAG) |
CNY 2,400.00 |
|
| SC324155 | YWHAG (untagged)-Human tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide (YWHAG) |
CNY 2,400.00 |
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