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MTMR8 (NM_017677) Human Tagged ORF Clone
CAT＃: RC201912
MTMR8 (Myc-DDK-tagged)-Human myotubularin related protein 8 (MTMR8)
ORF Plasmid: tGFP
"NM_017677" in other vectors (6)
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CNY 7,736.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC201912 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCATATTACGGTACCCAAGGTAGAAAACGTGAAATTGGTGGATCGTTATGTGAGTAAGAAACCAG CTAATGGGATTCTTTATCTTACTGCAACCCACCTGATCTATGTGGAGGCTTCAGGTGCAGCCCGGAAAGA AACATGGATTGCACTCCATCACATTGCCACTGTGGAGAAGTTACCCATCACTAGCCTGGGTTGTCCCCTG ACCCTCCGCTGCAAGAATTTCCGGGTGGCCCACTTTGTTTTAGATTCTGACCTTGTGTGCCATGAGGTTT ATATTTCACTGCTCAAGCTTTCTCAGCCAGCATTACCTGAAGATCTTTATGCTTTTTCTTATAATCCCAA ATCCTCAAAAGAGATGAGGGAAAGTGGATGGAAACTGATTGACCCAATATCAGACTTTGGGCGTATGGGA ATACCCAACAGAAACTGGACCATAACAGATGCCAACAGAAACTATGAGATATGCAGCACCTACCCTCCTG AAATAGTGGTTCCTAAATCTGTTACCTTGGGAACGGTGGTTGGAAGTTCAAAGTTCAGAAGTAAAGAACG TGTCCCTGTGCTCTCCTACCTCTACAAAGAGAACAATGCTGCCATTTGCCGCTGTAGCCAGCCTCTCTCT GGATTTTACACTCGCTGTGTAGATGATGAGCTCTTGTTGGAGGCCATTAGCCAAACAAACCCAGGGAGCC AGTTTATGTATGTTGTAGACACAAGACCAAAGTTGAATGCCATGGCCAACCGAGCAGCTGGGAAGGGGTA TGAAAATGAAGACAACTATGCCAACATTCGCTTCAGATTCATGGGCATTGAGAACATCCATGTAATGCGG AGCAGTCTGCAGAAACTCTTGGAAGTTTGTGAATTGAAAACTCCAACAATGAGTGAATTTCTTAGCGGCC TGGAGAGCTCAGGGTGGTTAAGACACATTAAAGCTATTATGGATGCTGGAATTTTCATTACAAAGGCAGT GAAGGTAGAAAAGGCCAGTGTCTTAGTCCATTGTTCTGATGGATGGGACCGCACAGCACAAGTCTGCTCA GTGGCTAGCATCCTCCTAGATCCATTTTATAGGACATTCAAAGGACTCATGATCTTGATAGAGAAGGAAT GGATATCCATGGGCCACAAGTTTTCCCAAAGGTGTGGCCACCTCGATGGGGACTCTAAAGAAGTGTCCCC TATCTTCACCCAGTTCCTAGACTGTATCTGGCAATTAATGGAACAGTTTCCCTGTGCCTTTGAGTTTAAT GAAAACTTCCTGCTGGAGATTCATGACCATGTTTTCTCCTGCCAGTTTGGAAACTTCCTTGGTAACTGCC AGAAGGATCGGGAAGATCTAAGAGTCTATGAGAAAACACATTCTGTGTGGCCTTTCTTGGTTCAGAGGAA ACCAGACTTCAGGAACCCTCTCTATAAAGGCTTCACTATGTATGGGGTACTCAATCCTAGTACTGTGCCC TACAACATTCAGTTCTGGTGTGGGATGTATAACCGCTTTGACAAAGGGCTGCAGCCCAAGCAGAGTATGC TAGAGAGCCTCCTGGAAATTAAGAAACAGAGAGCAATGCTGGAGACAGATGTGCATGAACTAGAAAAGAA ACTAAAAGTCCGTGATGAGCCACCAGAAGAGATCTGTACCTGCTCTCAATTAGGAAACATATTATCCCAG CATCTGGGAAGTCCTTTGACCAATCCTCTTGGCTTTATGGGTATCAATGGAGACCTGAATACCCTGATGG AGAATGGCACCCTATCCAGGGAAGGAGGCCTCCGAGCTCAGATGGATCAAGTAAAAAGCCAGGGTGCAGA CCTCCACCATAACTGTTGTGAGATTGTGGGCAGCCTTAGGGCCATAAATATCTCTGGGGATGTGGGCATC TCTGAGGCCATGGGCATCTCTGGAGACATGTGCACCTTTGAGGCTACGGGCTTCTCCAAAGACTTAGGAA TCTGTGGGGCCATGGACATCTCTGAGGCCACTGGCATCTCTGGAAACTTGGGTATTTCTGAGGCCAGGGG TTTCTCTGGGGACATGGGCATCTTGGGGGACACAGGCATCTCCAAGGCCAGCACCAAGGAGGCAGACTAC TCCAAGCATCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201912 protein sequence
Red=Cloning site Green=Tags(s) MDHITVPKVENVKLVDRYVSKKPANGILYLTATHLIYVEASGAARKETWIALHHIATVEKLPITSLGCPL TLRCKNFRVAHFVLDSDLVCHEVYISLLKLSQPALPEDLYAFSYNPKSSKEMRESGWKLIDPISDFGRMG IPNRNWTITDANRNYEICSTYPPEIVVPKSVTLGTVVGSSKFRSKERVPVLSYLYKENNAAICRCSQPLS GFYTRCVDDELLLEAISQTNPGSQFMYVVDTRPKLNAMANRAAGKGYENEDNYANIRFRFMGIENIHVMR SSLQKLLEVCELKTPTMSEFLSGLESSGWLRHIKAIMDAGIFITKAVKVEKASVLVHCSDGWDRTAQVCS VASILLDPFYRTFKGLMILIEKEWISMGHKFSQRCGHLDGDSKEVSPIFTQFLDCIWQLMEQFPCAFEFN ENFLLEIHDHVFSCQFGNFLGNCQKDREDLRVYEKTHSVWPFLVQRKPDFRNPLYKGFTMYGVLNPSTVP YNIQFWCGMYNRFDKGLQPKQSMLESLLEIKKQRAMLETDVHELEKKLKVRDEPPEEICTCSQLGNILSQ HLGSPLTNPLGFMGINGDLNTLMENGTLSREGGLRAQMDQVKSQGADLHHNCCEIVGSLRAINISGDVGI SEAMGISGDMCTFEATGFSKDLGICGAMDISEATGISGNLGISEARGFSGDMGILGDTGISKASTKEADY SKHQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_017677 |
| ORF Size | 2112 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_017677.4 |
| RefSeq Size | 2675 bp |
| RefSeq ORF | 2115 bp |
| Locus ID | 55613 |
| UniProt ID | Q96EF0 |
| Domains | PTPc_motif |
| Protein Families | Druggable Genome, Phosphatase |
| MW | 78.9 kDa |
| Gene Summary | This gene encodes a member of the myotubularin-related family and is part of the MTMR6 subgroup. Family members are dual-specificity phosphatases and the encoded protein contains a phosphoinositide-binding domain (PID) and a SET-interacting domain (SID). Defects in other family members have been found in myotubular myopathic diseases. [provided by RefSeq, Mar 2010] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
A conserved myotubularin-related phosphatase regulates autophagy by maintaining autophagic flux
,Allen, EA;Amato, C;Fortier, TM;Velentzas, P;Wood, W;Baehrecke, EH;,
J. Cell Biol.
,PubMed ID 32915229
[MTMR8]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201912L1 | Lenti ORF clone of Human myotubularin related protein 8 (MTMR8), Myc-DDK-tagged |
CNY 10,136.00 |
|
| RC201912L2 | Lenti ORF clone of Human myotubularin related protein 8 (MTMR8), mGFP tagged |
CNY 7,700.00 |
|
| RC201912L3 | Lenti ORF clone of Human myotubularin related protein 8 (MTMR8), Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC201912L4 | Lenti ORF clone of Human myotubularin related protein 8 (MTMR8), mGFP tagged |
CNY 7,700.00 |
|
| RG201912 | MTMR8 (tGFP-tagged) - Human myotubularin related protein 8 (MTMR8) |
CNY 6,370.00 |
|
| SC128053 | MTMR8 (untagged)-Human myotubularin related protein 8 (MTMR8) |
CNY 7,744.00 |
