FAM50B (NM_012135) Human Tagged ORF Clone
CAT#: RC201531
FAM50B (Myc-DDK-tagged)-Human family with sequence similarity 50, member B (FAM50B)
ORF Plasmid: tGFP
"NM_012135" in other vectors (5)
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CNY 2,400.00
CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | D6S2654E; X5L |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC201531 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCAGTACAAGGGCACCATGCGCGAGGCAGGCCGTGCCATGCACCTCCTCAAGAAGCGCGAAAGGC AGCGGGAGCAGATGGAGGTGCTGAAGCAGCGCATCGCCGAGGAGACCATCCTCAAGTCGCAGGTGGACAA GAGGTTCTCGGCGCATTACGACGCCGTGGAGGCCGAGCTGAAGTCCAGCACGGTGGGCCTGGTGACCCTG AACGACATGAAGGCCCGGCAGGAGGCCCTGGTCAGGGAGCGCGAGCGGCAGCTGGCCAAGCGCCAGCACC TGGAGGAGCAGCGGCTGCAGCAGGAGCGGCAGCGGGAGCAGGAGCAGCGGCGCGAGCGCAAGCGTAAGAT CTCCTGCCTGTCCTTTGCACTAGACGACCTCGATGACCAGGCCGACGCGGCCGAGGCCAGGCGCGCCGGA AACCTGGGCAAGAACCCCGACGTGGACACCAGCTTCCTGCCAGACCGCGACCGCGAGGAGGAGGAGAACC GGCTCCGAGAGGAGCTGCGCCAAGAGTGGGAGGCGCAGCGCGAGAAAGTGAAGGACGAGGAGATGGAGGT CACCTTCAGCTACTGGGACGGCTCGGGCCACCGGCGCACGGTGCGGGTGCGCAAGGGCAACACGGTGCAG CAGTTCCTGAAGAAGGCGCTGCAGGGGCTGCGCAAGGACTTCCTGGAGCTGCGCTCCGCCGGCGTGGAGC AGCTCATGTTCATCAAGGAGGACCTCATCCTGCCGCACTACCACACCTTCTACGACTTCATCATCGCCAG GGCGAGGGGCAAGAGCGGGCCGCTCTTCAGCTTCGATGTGCACGATGACGTGCGCCTGCTCAGCGACGCC ACCATGGAGAAGGACGAGTCGCACGCGGGCAAGGTGGTGCTGCGCAGCTGGTACGAGAAGAACAAGCACA TCTTCCCCGCCAGCCGCTGGGAGGCCTATGACCCCGAGAAGAAGTGGGACAAGTACACCATCCGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201531 protein sequence
Red=Cloning site Green=Tags(s) MAQYKGTMREAGRAMHLLKKRERQREQMEVLKQRIAEETILKSQVDKRFSAHYDAVEAELKSSTVGLVTL NDMKARQEALVRERERQLAKRQHLEEQRLQQERQREQEQRRERKRKISCLSFALDDLDDQADAAEARRAG NLGKNPDVDTSFLPDRDREEEENRLREELRQEWEAQREKVKDEEMEVTFSYWDGSGHRRTVRVRKGNTVQ QFLKKALQGLRKDFLELRSAGVEQLMFIKEDLILPHYHTFYDFIIARARGKSGPLFSFDVHDDVRLLSDA TMEKDESHAGKVVLRSWYEKNKHIFPASRWEAYDPEKKWDKYTIR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
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Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_012135 |
ORF Size | 975 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_012135.3 |
RefSeq Size | 1618 bp |
RefSeq ORF | 978 bp |
Locus ID | 26240 |
UniProt ID | Q9Y247 |
Domains | XAP5 |
MW | 38.7 kDa |
Gene Summary | This gene contains an intronless ORF that arose from ancestral retroposition. The encoded protein is related to a plant protein that plays a role in the circadian clock. This gene is adjacent to a differentially methylated region (DMR) and is imprinted and paternally expressed in many tissues. [provided by RefSeq, Nov 2015] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC201531L3 | Lenti ORF clone of Human family with sequence similarity 50, member B (FAM50B), Myc-DDK-tagged |
CNY 4,800.00 |
|
RC201531L4 | Lenti ORF clone of Human family with sequence similarity 50, member B (FAM50B), mGFP tagged |
CNY 4,800.00 |
|
RG201531 | FAM50B (tGFP-tagged) - Human family with sequence similarity 50, member B (FAM50B) |
CNY 4,000.00 |
|
SC115529 | FAM50B (untagged)-Human family with sequence similarity 50, member B (FAM50B) |
CNY 2,400.00 |
|
SC322209 | FAM50B (untagged)-Human family with sequence similarity 50, member B (FAM50B) |
CNY 2,400.00 |