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RPL8 (NM_033301) Human Tagged ORF Clone
CAT＃: RC201368
RPL8 (Myc-DDK-tagged)-Human ribosomal protein L8 (RPL8), transcript variant 2
ORF Plasmid: tGFP
"NM_033301" in other vectors (5)
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CNY 2,400.00
CNY 3,705.00
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CNY 300.00
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| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | L8 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC201368 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCCGTGTGATCCGTGGACAGAGGAAGGGCGCCGGGTCTGTGTTCCGCGCGCACGTGAAGCACCGTA AAGGCGCTGCGCGCCTGCGCGCCGTGGATTTCGCTGAGCGGCACGGCTACATCAAGGGCATCGTCAAGGA CATCATCCACGACCCGGGCCGCGGCGCGCCCCTCGCCAAGGTGGTCTTCCGGGATCCGTATCGGTTTAAG AAGCGGACGGAGCTGTTCATTGCCGCCGAGGGCATTCACACGGGCCAGTTTGTGTATTGCGGCAAGAAGG CCCAGCTCAACGTTGGCAATGTGCTCCCTGTGGGCACCATGCCTGAGGGTACAATCGTGTGCTGCCTGGA GGAGAAGCCTGGAGACCGTGGCAAGCTGGCCCGGGCATCAGGGAACTATGCCACCGTTATCTCCCACAAC CCTGAGACCAAGAAGACCCGTGTGAAGCTGCCCTCCGGCTCCAAGAAGGTTATCTCCTCAGCCAACAGAG CTGTGGTTGGTGTGGTGGCTGGAGGTGGCCGAATTGACAAACCCATCTTGAAGGCTGGCCGGGCGTACCA CAAATATAAGGCAAAGAGGAACTGCTGGCCACGAGTACGGGGTGTGGCCATGAATCCTGTGGAGCATCCT TTTGGAGGTGGCAACCACCAGCACATCGGCAAGCCCTCCACCATCCGCAGAGATGCCCCTGCTGGCCGCA AAGTGGGTCTCATTGCTGCCCGCCGGACTGGACGTCTCCGGGGAACCAAGACTGTGCAGGAGAAAGAGAA C ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201368 protein sequence
Red=Cloning site Green=Tags(s) MGRVIRGQRKGAGSVFRAHVKHRKGAARLRAVDFAERHGYIKGIVKDIIHDPGRGAPLAKVVFRDPYRFK KRTELFIAAEGIHTGQFVYCGKKAQLNVGNVLPVGTMPEGTIVCCLEEKPGDRGKLARASGNYATVISHN PETKKTRVKLPSGSKKVISSANRAVVGVVAGGGRIDKPILKAGRAYHKYKAKRNCWPRVRGVAMNPVEHP FGGGNHQHIGKPSTIRRDAPAGRKVGLIAARRTGRLRGTKTVQEKEN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_033301 |
| ORF Size | 771 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_033301.3 |
| RefSeq Size | 967 bp |
| RefSeq ORF | 774 bp |
| Locus ID | 6132 |
| UniProt ID | P62917 |
| Domains | Ribosomal_L2 |
| Protein Pathways | Ribosome |
| MW | 28 kDa |
| Gene Summary | Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L2P family of ribosomal proteins. It is located in the cytoplasm. In rat, the protein associates with the 5.8S rRNA, very likely participates in the binding of aminoacyl-tRNA, and is a constituent of the elongation factor 2-binding site at the ribosomal subunit interface. Alternatively spliced transcript variants encoding the same protein exist. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201368L3 | Lenti ORF clone of Human ribosomal protein L8 (RPL8), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201368L4 | Lenti ORF clone of Human ribosomal protein L8 (RPL8), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG201368 | RPL8 (tGFP-tagged) - Human ribosomal protein L8 (RPL8), transcript variant 2 |
CNY 4,000.00 |
|
| SC109743 | RPL8 (untagged)-Human ribosomal protein L8 (RPL8), transcript variant 2 |
CNY 3,990.00 |
|
| SC320862 | RPL8 (untagged)-Human ribosomal protein L8 (RPL8), transcript variant 2 |
CNY 2,400.00 |
