EIF2B2 (NM_014239) Human Tagged ORF Clone
CAT#: RC201351
EIF2B2 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 2B, subunit 2 beta, 39kDa (EIF2B2)
ORF Plasmid: tGFP
"NM_014239" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | EIF-2Bbeta; EIF2B |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC201351 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGGGATCCGCAGCGAAGGGCTCGGAGTTGTCAGAGAGGATCGAGAGCTTCGTGGAGACCCTGAAGC GGGGTGGTGGGCCGCGCAGCTCCGAGGAAATGGCTCGGGAGACCCTAGGGTTGCTGCGCCAGATCATCAC GGACCACCGCTGGAGCAACGCGGGGGAGCTGATGGAGCTGATCCGCAGAGAGGGCAGGAGGATGACGGCC GCTCAGCCCTCCGAGACCACCGTGGGCAACATGGTGCGGAGAGTGCTCAAGATTATCCGGGAGGAGTATG GCAGACTCCATGGACGCACCGACGAGAGTGATCAGCAGGAGTCCCTGCACAAACTGTTGACATCCGGAGG CCTAAACGAGGATTTCAGCTTCCATTATGCCCAACTCCAGTCCAACATCATTGAGGCGATTAATGAGCTG CTAGTGGAGCTGGAAGGGACAATGGAGAACATTGCAGCCCAGGCTCTGGAGCACATTCACTCCAATGAGG TGATCATGACCATTGGCTTCTCCCGAACAGTAGAGGCCTTCCTCAAAGAGGCTGCCCGAAAGAGGAAATT CCATGTCATTGTAGCAGAGTGTGCTCCTTTCTGCCAGGGTCATGAAATGGCTGTGAATTTGTCCAAAGCA GGTATTGAGACAACTGTCATGACTGATGCTGCCATTTTTGCCGTTATGTCAAGAGTCAACAAGGTGATCA TTGGCACGAAGACCATCCTGGCCAATGGGGCCCTGAGAGCTGTGACAGGAACTCACACTCTGGCACTGGC AGCAAAACACCATTCCACCCCACTCATCGTCTGTGCACCTATGTTCAAACTTTCTCCACAGTTCCCCAAT GAAGAAGACTCATTTCATAAGTTTGTGGCTCCTGAAGAAGTCCTGCCATTCACAGAAGGGGACATTCTGG AGAAGGTCAGCGTGCATTGCCCTGTGTTTGACTACGTTCCCCCAGAGCTCATTACCCTCTTTATCTCCAA CATTGGTGGGAATGCACCTTCCTACATCTACCGCCTGATGAGTGAACTCTACCATCCTGATGATCATGTT TTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201351 protein sequence
Red=Cloning site Green=Tags(s) MPGSAAKGSELSERIESFVETLKRGGGPRSSEEMARETLGLLRQIITDHRWSNAGELMELIRREGRRMTA AQPSETTVGNMVRRVLKIIREEYGRLHGRTDESDQQESLHKLLTSGGLNEDFSFHYAQLQSNIIEAINEL LVELEGTMENIAAQALEHIHSNEVIMTIGFSRTVEAFLKEAARKRKFHVIVAECAPFCQGHEMAVNLSKA GIETTVMTDAAIFAVMSRVNKVIIGTKTILANGALRAVTGTHTLALAAKHHSTPLIVCAPMFKLSPQFPN EEDSFHKFVAPEEVLPFTEGDILEKVSVHCPVFDYVPPELITLFISNIGGNAPSYIYRLMSELYHPDDHV L TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_014239 |
ORF Size | 1053 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_014239.4 |
RefSeq Size | 1557 bp |
RefSeq ORF | 1056 bp |
Locus ID | 8892 |
UniProt ID | P49770 |
Domains | IF-2B |
MW | 39 kDa |
Gene Summary | This gene encodes the beta subunit of eukaryotic initiation factor-2B (EIF2B). EIF2B is involved in protein synthesis and exchanges GDP and GTP for its activation and deactivation. [provided by RefSeq, Aug 2011] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC201351L1 | Lenti ORF clone of Human eukaryotic translation initiation factor 2B, subunit 2 beta, 39kDa (EIF2B2), Myc-DDK-tagged |
CNY 6,056.00 |
|
RC201351L2 | Lenti ORF clone of Human eukaryotic translation initiation factor 2B, subunit 2 beta, 39kDa (EIF2B2), mGFP tagged |
CNY 5,890.00 |
|
RC201351L3 | Lenti ORF clone of Human eukaryotic translation initiation factor 2B, subunit 2 beta, 39kDa (EIF2B2), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC201351L4 | Lenti ORF clone of Human eukaryotic translation initiation factor 2B, subunit 2 beta, 39kDa (EIF2B2), mGFP tagged |
CNY 5,890.00 |
|
RG201351 | EIF2B2 (tGFP-tagged) - Human eukaryotic translation initiation factor 2B, subunit 2 beta, 39kDa (EIF2B2) |
CNY 5,256.00 |
|
SC115107 | EIF2B2 (untagged)-Human eukaryotic translation initiation factor 2B, subunit 2 beta, 39kDa (EIF2B2) |
CNY 3,656.00 |