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GDF15 (NM_004864) Human Tagged ORF Clone
CAT＃: RC201295
GDF15 (Myc-DDK-tagged)-Human growth differentiation factor 15 (GDF15)
ORF Plasmid: tGFP
"NM_004864" in other vectors (6)
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CNY 3,600.00
CNY 3,990.00
| Cited in 3 publications. |
Product images
CNY 300.00
热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GDF-15; MIC-1; MIC1; NAG-1; PDF; PLAB; PTGFB |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC201295 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCGGGCAAGAACTCAGGACGGTGAATGGCTCTCAGATGCTCCTGGTGTTGCTGGTGCTCTCGTGGC TGCCGCATGGGGGCGCCCTGTCTCTGGCCGAGGCGAGCCGCGCAAGTTTCCCGGGACCCTCAGAGTTGCA CTCCGAAGACTCCAGATTCCGAGAGTTGCGGAAACGCTACGAGGACCTGCTAACCAGGCTGCGGGCCAAC CAGAGCTGGGAAGATTCGAACACCGACCTCGTCCCGGCCCCTGCAGTCCGGATACTCACGCCAGAAGTGC GGCTGGGATCCGGCGGCCACCTGCACCTGCGTATCTCTCGGGCCGCCCTTCCCGAGGGGCTCCCCGAGGC CTCCCGCCTTCACCGGGCTCTGTTCCGGCTGTCCCCGACGGCGTCAAGGTCGTGGGACGTGACACGACCG CTGCGGCGTCAGCTCAGCCTTGCAAGACCCCAGGCGCCCGCGCTGCACCTGCGACTGTCGCCGCCGCCGT CGCAGTCGGACCAACTGCTGGCAGAATCTTCGTCCGCACGGCCCCAGCTGGAGTTGCACTTGCGGCCGCA AGCCGCCAGGGGGCGCCGCAGAGCGCGTGCGCGCAACGGGGACCACTGTCCGCTCGGGCCCGGGCGTTGC TGCCGTCTGCACACGGTCCGCGCGTCGCTGGAAGACCTGGGCTGGGCCGATTGGGTGCTGTCGCCACGGG AGGTGCAAGTGACCATGTGCATCGGCGCGTGCCCGAGCCAGTTCCGGGCGGCAAACATGCACGCGCAGAT CAAGACGAGCCTGCACCGCCTGAAGCCCGACACGGTGCCAGCGCCCTGCTGCGTGCCCGCCAGCTACAAT CCCATGGTGCTCATTCAAAAGACCGACACCGGGGTGTCGCTCCAGACCTATGATGACTTGTTAGCCAAAG ACTGCCACTGCATA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201295 protein sequence
Red=Cloning site Green=Tags(s) MPGQELRTVNGSQMLLVLLVLSWLPHGGALSLAEASRASFPGPSELHSEDSRFRELRKRYEDLLTRLRAN QSWEDSNTDLVPAPAVRILTPEVRLGSGGHLHLRISRAALPEGLPEASRLHRALFRLSPTASRSWDVTRP LRRQLSLARPQAPALHLRLSPPPSQSDQLLAESSSARPQLELHLRPQAARGRRRARARNGDHCPLGPGRC CRLHTVRASLEDLGWADWVLSPREVQVTMCIGACPSQFRAANMHAQIKTSLHRLKPDTVPAPCCVPASYN PMVLIQKTDTGVSLQTYDDLLAKDCHCI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004864 |
| ORF Size | 924 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004864.4 |
| RefSeq Size | 1220 bp |
| RefSeq ORF | 927 bp |
| Locus ID | 9518 |
| UniProt ID | Q99988 |
| Domains | TGF-beta |
| Protein Families | Druggable Genome, Secreted Protein |
| MW | 34.1 kDa |
| Gene Summary | This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. The protein is expressed in a broad range of cell types, acts as a pleiotropic cytokine and is involved in the stress response program of cells after cellular injury. Increased protein levels are associated with disease states such as tissue hypoxia, inflammation, acute injury and oxidative stress. [provided by RefSeq, Aug 2016] |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Parental metabolic syndrome epigenetically reprograms offspring hepatic lipid metabolism in mice
,De Jesus, DF;Orime, K;Kaminska, D;Kimura, T;Basile, G;Wang, CH;Haertle, L;Riemens, R;Brown, NK;Hu, J;Männistö, V;Silva, AM;Dirice, E;Tseng, YH;Haaf, T;Pihlajamäki, J;Kulkarni, RN;,
J. Clin. Invest.
,PubMed ID 32250344
[GDF15]
|
|
Growth differentiation factor 15 mediates epithelial mesenchymal transition and invasion of breast cancers through IGF-1R-FoxM1 signaling
,Peake, BF;Eze, SM;Yang, L;Castellino, RC;Nahta, R;,
Oncotarget
,PubMed ID 29212236
[GDF15]
|
|
P38 MAPK contributes to resistance and invasiveness of HER2- overexpressing breast cancer
,Donnelly, SM;Paplomata, E;Peake, BM;Sanabria, E;Chen, Z;Nahta, R;,
Curr. Med. Chem., Dec 2013.
,PubMed ID 24251561
[GDF15]
|
Documents
| Product Manuals |
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201295L1 | Lenti ORF clone of Human growth differentiation factor 15 (GDF15), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201295L2 | Lenti ORF clone of Human growth differentiation factor 15 (GDF15), mGFP tagged |
CNY 6,000.00 |
|
| RC201295L3 | Lenti ORF clone of Human growth differentiation factor 15 (GDF15), Myc-DDK-tagged |
CNY 6,000.00 |
|
| RC201295L4 | Lenti ORF clone of Human growth differentiation factor 15 (GDF15), mGFP tagged |
CNY 6,000.00 |
|
| RG201295 | GDF15 (tGFP-tagged) - Human growth differentiation factor 15 (GDF15) |
CNY 5,200.00 |
|
| SC319494 | GDF15 (untagged)-Human growth differentiation factor 15 (GDF15) |
CNY 3,600.00 |
