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LDB1 (NM_003893) Human Tagged ORF Clone
CAT＃: RC201182
LDB1 (Myc-DDK-tagged)-Human LIM domain binding 1 (LDB1), transcript variant 3
ORF Plasmid: tGFP
"NM_003893" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CLIM-2; CLIM2; LDB-1; NLI |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC201182 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGGATAGGGATGTGGGCCCAACTCCCATGTATCCGCCTACATACCTGGAGCCAGGGATTGGGAGGC ACACACCATATGGCAACCAAACTGACTACAGAATATTTGAGCTTAACAAACGGCTTCAGAACTGGACAGA GGAGTGTGACAATCTCTGGTGGGATGCATTCACGACTGAGTTCTTTGAGGATGATGCCATGTTGACCATC ACTTTCTGCCTGGAGGATGGACCAAAGAGATATACCATTGGCCGGACCCTGATCCCACGCTACTTCCGCA GCATCTTTGAGGGGGGTGCTACGGAGCTGTACTATGTTCTTAAGCACCCCAAGGAGGCATTCCACAGCAA CTTTGTGTCCCTCGACTGTGACCAGGGCAGCATGGTGACCCAGCATGGCAAGCCCATGTTCACCCAGGTG TGTGTGGAGGGCCGGTTGTACCTGGAGTTCATGTTTGACGACATGATGCGGATAAAGACGTGGCACTTCA GCATCCGGCAGCACCGAGAGCTCATCCCCCGCAGCATCCTTGCCATGCATGCCCAAGACCCCCAGATGTT GGATCAGCTCTCCAAAAACATCACTCGGTGTGGGCTGTCCAATTCCACTCTCAACTACCTCCGACTCTGT GTGATACTCGAGCCCATGCAAGAGCTCATGTCACGCCACAAGACCTACAGCCTCAGCCCCCGCGACTGCC TCAAGACCTGCCTTTTCCAGAAGTGGCAGCGCATGGTAGCACCCCCTGCGGAGCCCACACGTCAGCAGCC CAGCAAACGGCGGAAACGGAAGATGTCAGGGGGCAGCACCATGAGCTCTGGTGGTGGCAACACCAACAAC AGCAACAGCAAGAAGAAGAGCCCAGCTAGCACCTTCGCCCTCTCCAGCCAGGTACCTGATGTGATGGTGG TGGGGGAGCCCACCCTGATGGGCGGGGAGTTCGGGGACGAGGACGAGAGGCTCATCACCCGGCTGGAGAA CACCCAGTTTGACGCAGCCAACGGCATTGACGACGAGGACAGCTTTAACAACTCCCCTGCACTGGGCGCC AACAGCCCCTGGAACAGCAAGCCTCCGTCCAGCCAAGAAAGCAAATCGGAGAACCCCACGTCACAGGCCT CCCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201182 protein sequence
Red=Cloning site Green=Tags(s) MLDRDVGPTPMYPPTYLEPGIGRHTPYGNQTDYRIFELNKRLQNWTEECDNLWWDAFTTEFFEDDAMLTI TFCLEDGPKRYTIGRTLIPRYFRSIFEGGATELYYVLKHPKEAFHSNFVSLDCDQGSMVTQHGKPMFTQV CVEGRLYLEFMFDDMMRIKTWHFSIRQHRELIPRSILAMHAQDPQMLDQLSKNITRCGLSNSTLNYLRLC VILEPMQELMSRHKTYSLSPRDCLKTCLFQKWQRMVAPPAEPTRQQPSKRRKRKMSGGSTMSSGGGNTNN SNSKKKSPASTFALSSQVPDVMVVGEPTLMGGEFGDEDERLITRLENTQFDAANGIDDEDSFNNSPALGA NSPWNSKPPSSQESKSENPTSQASQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003893 |
| ORF Size | 1125 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003893.5 |
| RefSeq Size | 2305 bp |
| RefSeq ORF | 1128 bp |
| Locus ID | 8861 |
| UniProt ID | Q86U70 |
| Domains | LIM_bind |
| Protein Families | Transcription Factors |
| MW | 42.8 kDa |
| Gene Summary | Binds to the LIM domain of a wide variety of LIM domain-containing transcription factors. May regulate the transcriptional activity of LIM-containing proteins by determining specific partner interactions. Plays a role in the development of interneurons and motor neurons in cooperation with LHX3 and ISL1. Acts synergistically with LHX1/LIM1 in axis formation and activation of gene expression. Acts with LMO2 in the regulation of red blood cell development, maintaining erythroid precursors in an immature state (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201182L3 | Lenti ORF clone of Human LIM domain binding 1 (LDB1), transcript variant 3, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201182L4 | Lenti ORF clone of Human LIM domain binding 1 (LDB1), transcript variant 3, mGFP tagged |
CNY 5,890.00 |
|
| RG201182 | LDB1 (tGFP-tagged) - Human LIM domain binding 1 (LDB1), transcript variant 3 |
CNY 5,256.00 |
|
| SC117666 | LDB1 (untagged)-Human LIM domain binding 1 (LDB1), transcript variant 3 |
CNY 3,656.00 |
