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LDB1 (NM_003893) Human Untagged Clone
CAT＃: SC117666
LDB1 (untagged)-Human LIM domain binding 1 (LDB1), transcript variant 3
CNY 3,656.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CLIM-2; CLIM2; LDB-1; NLI |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_003893, the custom clone sequence may differ by one or more nucleotides
ATGCTGGATAGGGATGTGGGCCCAACTCCCATGTATCCGCCTACATACCTGGAGCCAGGGATTGGGAGGC ACACACCATATGGCAACCAAACTGACTACAGAATATTTGAGCTTAACAAACGGCTTCAGAACTGGACAGA GGAGTGTGACAATCTCTGGTGGGATGCATTCACGACTGAGTTCTTTGAGGATGATGCCATGTTGACCATC ACTTTCTGCCTGGAGGATGGACCAAAGAGATATACCATTGGCCGGACCCTGATCCCACGCTACTTCCGCA GCATCTTTGAGGGGGGTGCTACGGAGCTGTACTATGTTCTTAAGCACCCCAAGGAGGCATTCCACAGCAA CTTTGTGTCCCTCGACTGTGACCAGGGCAGCATGGTGACCCAGCATGGCAAGCCCATGTTCACCCAGGTG TGTGTGGAGGGCCGGTTGTACCTGGAGTTCATGTTTGACGACATGATGCGGATAAAGACGTGGCACTTCA GCATCCGGCAGCACCGAGAGCTCATCCCCCGCAGCATCCTTGCCATGCATGCCCAAGACCCCCAGATGTT GGATCAGCTCTCCAAAAACATCACTCGGTGTGGGCTGTCCAATTCCACTCTCAACTACCTCCGACTCTGT GTGATACTCGAGCCCATGCAAGAGCTCATGTCACGCCACAAGACCTACAGCCTCAGCCCCCGCGACTGCC TCAAGACCTGCCTTTTCCAGAAGTGGCAGCGCATGGTAGCACCCCCTGCGGAGCCCACACGTCAGCAGCC CAGCAAACGGCGGAAACGGAAGATGTCAGGGGGCAGCACCATGAGCTCTGGTGGTGGCAACACCAACAAC AGCAACAGCAAGAAGAAGAGCCCAGCTAGCACCTTCGCCCTCTCCAGCCAGGTACCTGATGTGATGGTGG TGGGGGAGCCCACCCTGATGGGCGGGGAGTTCGGGGACGAGGACGAGAGGCTCATCACCCGGCTGGAGAA CACCCAGTTTGACGCAGCCAACGGCATTGACGACGAGGACAGCTTTAACAACTCCCCTGCACTGGGCGCC AACAGCCCCTGGAACAGCAAGCCTCCGTCCAGCCAAGAAAGCAAATCGGAGAACCCCACGTCACAGGCCT CCCAGTAA >OriGene 5' read for NM_003893 unedited
ATGTCANCATTTGTATACGACNACTATAGGCGGCACGCGAATTCGCACCAGCGGCGGCGG CTCATGCCCGGCCTGTCCCCGCGAGGGGCGGGCACCGTGACTCGGCTGGGCCCCCAGAGG CGGGCGATGTGAAGATGTCAGTGGGCTGTGCCTGTCCTGGTTGTTCCTCAAAGTCATTCA AGCTGTACTCGCCGAAGGAGCCCCCGAACGGCAACGCCTTTCCCCCCTTCCATCCCGGCA CCATGCTGGATAGGGATGTGGGCCCAACTCCCATGTATCCGCCTACATACCTGGAGCCAG GGATTGGGAGGCACACACCATATGGCAACCAAACTGACTACAGAATATTTGAGCTTAACA AACGGCTTCAGAACTGGACAGAGGAGTGTGACAATCTCTGGTGGGATGCATTCACGACTG AGTTCTTTGAGGATGATGCCATGTTGACCATCACTTTCTGCCTGGAGGATGGACCAAAGA GATATACCATTGGCCGGACCCTGATCCCACGCTACTTCCGCAGCATCTTTGAGGGGGGTG CTACGGAGCTGTACTATGTTCTTAAGCACCCCAAGGAGGCATTCCACAGCAACTTTGTGT CCCTCGACTGTGACCAGGGCAGCATGGTGACCCATCATGGCAAGCCCATGTTCACCCATG TGTGTGTGGAGGGCCGGTTGTACCTGGAGTTCATGTTTGACGACATGATGCGGATAAAGA CGTGGCACTTCAGCATCCGGCAGCACCGATAGCTCATCCCCCGCATCATCCTTGCCATGC ATGCNCAAGACCCCCAGATGNTGGNATCAGCTCTCCAAAACATCACTCGGGTGTGGGCTG TCCAATTCCACTCTCAACTACCTNCGACTCTGTGTGATACTCGAGCCCATGCATGATCTC ATGTCACGCCACATGATCTACAGNCTCATNCCNCGCGACTGGCTCT >OriGene 3' read for NM_003893 unedited
GAAAATCTAGNNACCGCGGCCGCAATCTAGNGATCGATTTTTTTTTTTTTTTTTTAACTT ACATTTTTAATAATATTATTTTTAAAAAAACTTGGAGCTGGGATAAGTGGCAGCAGGGAG GAGGGGCCCAGAGCTTTACCCCTCTATTACCAGCTGCCTAGGGGAAGGAGCATTCAACGA AGCCCCGTAACTTTAAGTCCCTAAGGGCTGTGGGTATAGACAACCCCAGGCTTGAAAGGG GTAAAGTCAGGGGGATGGGAAACCCACAATCTGGGGTGAAGATGGAGGCAAATGCCCTGG GGGGTGGTCAGGACATGTCTCAGAGGCCCAGGTTCCAAGTAGGCATCCACATGAGTACCC CCTCCCCCTAAAAGGCTCTGTAGAGGCCAGGCCCAGCCCAGGGCCACTGGGGGGGCAAAT CTTGGCACCTGCCCCCAGAGAGTCCAGTTCCTCCCTGAAGCGGGTGGATGGAGGCTGCCC ATTTTAGATGCTCAGTCTCTTCATTCTGTCTTCTGCTCCCTGGGGCTGTGAGGGGTGGGG CAGGTAGGCAGAGCACTGGGTGGCCACAGCAGGGCCTTTTACTGGGAGGCCTGTGACGTG GGGTTCTCCGATTTGCTTTCTTGGCTGGACGGAGGCTTGCTGTTCCAGGGGCTGTTGGCG CCCAGTGCAGGGGAGTTGTTAAAGCTGTCCTCGTCGTCAATGCCGTTGGCTGCGTCAAAC TGGGTGTTCTCCAGCCGGGTGATAAGCCTCTCGTCCTCGTCCCCGAATCCCCGCCCATCA GGGTGGGCTCCACCCCACCCTCACATCAAGTACCTGGCTGGAAAGGCCAAAGTGCTACCT GGGCCCTTCTTCTTGCTGTTGCTGGTGTGGGGGTGGCCACCACACNAAGCTAAGGTGCTG GCCCCCTGGCATCT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_003893 |
| Insert Size | 1900 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003893.3, NP_003884.1 |
| RefSeq Size | 2292 bp |
| RefSeq ORF | 1128 bp |
| Locus ID | 8861 |
| UniProt ID | Q86U70 |
| Domains | LIM_bind |
| Protein Families | Transcription Factors |
| Gene Summary | Binds to the LIM domain of a wide variety of LIM domain-containing transcription factors. May regulate the transcriptional activity of LIM-containing proteins by determining specific partner interactions. Plays a role in the development of interneurons and motor neurons in cooperation with LHX3 and ISL1. Acts synergistically with LHX1/LIM1 in axis formation and activation of gene expression. Acts with LMO2 in the regulation of red blood cell development, maintaining erythroid precursors in an immature state (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) has a different 5'-terminal exon and 5' UTR, compared to variant 1. This variant encodes a protein (isoform 3) that lacks 36 aa at the N-terminus, compared to isoform 1. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Regulation of Estrogen-Dependent Transcription by the LIM Cofactors CLIM and RLIM in Breast Cancer
,Steven A. Johnsen, Cenap Güngör, Tanja Prenzel, Sabine Riethdorf, Lutz Riethdorf, Naoko Taniguchi-Ishigaki, Thomas Rau, Baris Tursun, J. David Furlow, Guido Sauter, Martin Scheffner, Klaus Pantel, Frank Gannon, and Ingolf Bach,
Cancer Res., Jan 2009; 69: 128 - 136.
[LDB1]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201182 | LDB1 (Myc-DDK-tagged)-Human LIM domain binding 1 (LDB1), transcript variant 3 |
CNY 3,656.00 |
|
| RC201182L3 | Lenti ORF clone of Human LIM domain binding 1 (LDB1), transcript variant 3, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201182L4 | Lenti ORF clone of Human LIM domain binding 1 (LDB1), transcript variant 3, mGFP tagged |
CNY 5,890.00 |
|
| RG201182 | LDB1 (tGFP-tagged) - Human LIM domain binding 1 (LDB1), transcript variant 3 |
CNY 5,256.00 |
