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KEL (NM_000420) Human Tagged ORF Clone
CAT＃: RC201127
KEL (Myc-DDK-tagged)-Human Kell blood group, metallo-endopeptidase (KEL)
ORF Plasmid: tGFP
"NM_000420" in other vectors (6)
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CNY 5,360.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CD238; ECE3; Kell |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC201127 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGGTGGGGACCAAAGTGAGGAAGAGCCGAGGGAACGCAGCCAGGCAGGTGGAATGGGAACTCTCT GGAGCCAAGAGAGCACTCCAGAAGAGAGGCTGCCCGTGGAAGGGAGCAGGCCATGGGCAGTGGCCAGGCG GGTGCTGACAGCTATCCTGATTTTGGGCCTGCTCCTTTGTTTTTCTGTGCTTTTGTTCTACAACTTCCAG AACTGTGGCCCTCGCCCCTGTGAGACATCTGTGTGTTTGGATCTCCGGGATCATTACCTGGCCTCTGGGA ACACAAGTGTGGCCCCCTGCACCGACTTCTTCAGCTTTGCCTGTGGAAGGGCCAAAGAGACCAATAATTC TTTTCAGGAGCTTGCCACAAAGAACAAAAACCGACTTCGGAGAATACTGGAGGTCCAGAATTCCTGGCAC CCAGGCTCTGGGGAGGAGAAAGCCTTCCAGTTCTACAACTCCTGCATGGATACACTTGCCATTGAAGCTG CAGGGACTGGTCCCCTCAGACAAGTTATTGAGGAGCTTGGAGGCTGGCGCATCTCTGGTAAATGGACTTC CTTAAACTTTAACCGAACGCTGAGACTTCTGATGAGTCAGTATGGCCATTTCCCTTTCTTCAGAGCCTAC CTAGGACCTCATCCTGCCTCTCCACACACACCAGTCATCCAGATAGACCAGCCAGAGTTTGATGTTCCCC TCAAGCAAGATCAAGAACAGAAGATCTATGCCCAGATCTTTCGGGAATACCTGACTTACCTGAATCAGCT GGGAACCTTGCTGGGAGGAGACCCAAGCAAGGTGCAAGAACACTCTTCCTTGTCAATCTCCATCACTTCA CGGCTGTTCCAGTTTCTGAGGCCCCTGGAGCAGCGGCGGGCACAGGGCAAGCTCTTCCAGATGGTCACTA TCGACCAGCTCAAGGAAATGGCCCCCGCCATCGACTGGTTGTCCTGCTTGCAAGCGACATTCACACCGAT GTCCCTGAGCCCTTCTCAGTCCCTCGTGGTCCATGACGTGGAATATTTGAAAAACATGTCACAACTGGTG GAGGAGATGCTGCTAAAGCAGAGGGACTTTCTGCAGAGCCACATGATCTTAGGGCTGGTGGTGACCCTTT CTCCAGCCCTGGACAGTCAATTCCAGGAGGCACGCAGAAAGCTCAGCCAGAAACTGCGGGAACTGACAGA GCAACCACCCATGCCTGCCCGCCCACGATGGATGAAGTGCGTGGAGGAGACAGGCACGTTCTTCGAGCCC ACGCTGGCGGCTTTGTTTGTTCGTGAGGCCTTTGGCCCGAGCACCCGAAGTGCTGCCATGAAATTATTCA CTGCGATCCGGGATGCCCTCATCACTCGCCTCAGAAACCTTCCCTGGATGAATGAGGAGACCCAGAACAT GGCCCAGGACAAGGTTGCTCAACTGCAGGTGGAGATGGGGGCTTCAGAATGGGCCCTGAAGCCAGAGCTG GCCCGACAAGAATACAACGATATACAGCTTGGATCGAGCTTCCTGCAGTCTGTCCTGAGCTGTGTCCGGT CCCTCCGAGCTAGAATTGTCCAGAGCTTCTTGCAGCCTCACCCCCAACACAGGTGGAAGGTGTCCCCTTG GGACGTCAATGCTTACTATTCGGTATCTGACCATGTGGTAGTCTTTCCAGCTGGACTCCTCCAACCCCCA TTCTTCCACCCTGGCTATCCCAGAGCCGTGAACTTTGGCGCTGCTGGCAGCATCATGGCCCACGAGCTGT TGCACATCTTCTACCAGCTCTTACTGCCTGGGGGCTGCCTCGCCTGTGACAACCATGCCCTCCAGGAAGC TCACCTGTGCCTGAAGCGCCATTATGCTGCCTTTCCATTACCTAGCAGAACCTCCTTCAATGACTCCCTC ACATTCTTAGAGAATGCTGCAGACGTTGGGGGGCTAGCCATCGCGCTGCAGGCATACAGCAAGAGGCTGT TACGGCACCATGGGGAGACTGTCCTGCCCAGCCTGGACCTCAGCCCCCAGCAGATCTTCTTTCGAAGCTA TGCCCAGGTGATGTGTAGGAAGCCCAGCCCCCAGGACTCTCACGACACTCACAGCCCTCCACACCTCCGA GTCCACGGGCCCCTCAGCAGCACCCCAGCCTTTGCCAGGTATTTCCGCTGTGCACGTGGTGCTCTCTTGA ACCCCTCCAGCCGCTGCCAGCTCTGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201127 protein sequence
Red=Cloning site Green=Tags(s) MEGGDQSEEEPRERSQAGGMGTLWSQESTPEERLPVEGSRPWAVARRVLTAILILGLLLCFSVLLFYNFQ NCGPRPCETSVCLDLRDHYLASGNTSVAPCTDFFSFACGRAKETNNSFQELATKNKNRLRRILEVQNSWH PGSGEEKAFQFYNSCMDTLAIEAAGTGPLRQVIEELGGWRISGKWTSLNFNRTLRLLMSQYGHFPFFRAY LGPHPASPHTPVIQIDQPEFDVPLKQDQEQKIYAQIFREYLTYLNQLGTLLGGDPSKVQEHSSLSISITS RLFQFLRPLEQRRAQGKLFQMVTIDQLKEMAPAIDWLSCLQATFTPMSLSPSQSLVVHDVEYLKNMSQLV EEMLLKQRDFLQSHMILGLVVTLSPALDSQFQEARRKLSQKLRELTEQPPMPARPRWMKCVEETGTFFEP TLAALFVREAFGPSTRSAAMKLFTAIRDALITRLRNLPWMNEETQNMAQDKVAQLQVEMGASEWALKPEL ARQEYNDIQLGSSFLQSVLSCVRSLRARIVQSFLQPHPQHRWKVSPWDVNAYYSVSDHVVVFPAGLLQPP FFHPGYPRAVNFGAAGSIMAHELLHIFYQLLLPGGCLACDNHALQEAHLCLKRHYAAFPLPSRTSFNDSL TFLENAADVGGLAIALQAYSKRLLRHHGETVLPSLDLSPQQIFFRSYAQVMCRKPSPQDSHDTHSPPHLR VHGPLSSTPAFARYFRCARGALLNPSSRCQLW TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000420 |
| ORF Size | 2196 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000420.3 |
| RefSeq Size | 2562 bp |
| RefSeq ORF | 2199 bp |
| Locus ID | 3792 |
| UniProt ID | P23276 |
| Domains | Peptidase_M13 |
| Protein Families | Druggable Genome, Protease, Transmembrane |
| MW | 82.8 kDa |
| Gene Summary | This gene encodes a type II transmembrane glycoprotein that is the highly polymorphic Kell blood group antigen. The Kell glycoprotein links via a single disulfide bond to the XK membrane protein that carries the Kx antigen. The encoded protein contains sequence and structural similarity to members of the neprilysin (M13) family of zinc endopeptidases. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201127L1 | Lenti ORF clone of Human Kell blood group, metallo-endopeptidase (KEL), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201127L2 | Lenti ORF clone of Human Kell blood group, metallo-endopeptidase (KEL), mGFP tagged |
CNY 5,890.00 |
|
| RC201127L3 | Lenti ORF clone of Human Kell blood group, metallo-endopeptidase (KEL), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201127L4 | Lenti ORF clone of Human Kell blood group, metallo-endopeptidase (KEL), mGFP tagged |
CNY 5,890.00 |
|
| RG201127 | KEL (tGFP-tagged) - Human Kell blood group, metallo-endopeptidase (KEL) |
CNY 4,370.00 |
|
| SC119878 | KEL (untagged)-Human Kell blood group, metallo-endopeptidase (KEL) |
CNY 5,368.00 |
