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LARP1 (NM_015315) Human Tagged ORF Clone
CAT＃: RC200935
- TrueORF®
LARP1 (Myc-DDK-tagged)-Human La ribonucleoprotein domain family, member 1 (LARP1), transcript variant 1
ORF Plasmid: tGFP
"NM_015315" in other vectors (6)
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CNY 7,096.00
CNY 7,600.00
| Cited in 5 publications. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Lar1; LARP; Lhp1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC200935 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTTTGGAGGGTGCTTTTGTCAAAGAGGCCTCCTTTCCCTCACCCAGAGCTGGATTTCCAAGAGGCTC CCATACCTAGCTGCCCTGGCAGACTCCCAGGGAGGAAAAACAGCGTGGCCTTGGCAGCTGCCCCGAGGAA GGAGCCCACAGGTGACAGGGAGAAGCCATTGCCATTCCCTGTCCTGGCCCCCTTCAGCAACCCTGAACAC TCTGCTCCAGCCAAGGTGGTGAGGGCAGCTGTTCCTAAACAGCGCAAAGGCAGCAAGGTTGGTGACTTTG GAGATGCAATCAATTGGCCCACACCTGGAGAGATAGCCCACAAGAGTGTTCAGCCACAGTCCCACAAGCC TCAGCCTACCCGTAAACTGCCACCCAAGAAGGACATGAAGGAACAGGAGAAAGGAGAAGGGAGTGATAGT AAGGAGAGTCCAAAAACCAAATCAGATGAATCAGGGGAGGAAAAGAATGGAGATGAGGATTGCCAGCGAG GCGGGCAGAAGAAGAAAGGAAACAAACACAAGTGGGTTCCATTACAAATAGACATGAAGCCTGAAGTGCC CAGAGAGAAACTGGCTTCACGCCCCACTCGCCCACCGGAGCCTAGACACATACCTGCCAATCGCGGAGAG ATCAAAGGGTCTGAGTCTGCCACCTACGTGCCCGTGGCCCCCCCCACCCCAGCCTGGCAACCAGAGATCA AACCGGAGCCTGCCTGGCACGACCAGGATGAGACATCGAGTGTGAAGAGTGATGGGGCTGGTGGGGCGCG GGCTTCCTTCCGTGGCCGTGGACGGGGGCGTGGTCGCGGCCGGGGACGCGGCCGGGGTGGCACTCGAACC CATTTTGACTACCAGTTTGGCTACCGAAAGTTTGATGGTGTGGAGGGGCCTCGTACGCCCAAGTACATGA ACAACATCACCTACTACTTTGACAATGTCAGCAGCACCGAGCTTTACAGTGTGGATCAGGAACTGCTCAA AGACTACATCAAGCGCCAGATTGAATACTACTTCAGCGTGGACAATTTAGAGCGAGACTTCTTCCTGCGA AGGAAAATGGATGCTGATGGTTTCCTACCCATCACCCTTATTGCTTCCTTCCACCGAGTGCAGGCCCTTA CCACTGACATTTCACTCATCTTTGCGGCCCTAAAGGACAGCAAGGTGGTGGAGATCGTTGATGAGAAAGT TCGTAGGAGGGAGGAACCAGAAAAGTGGCCTCTTCCCCCAATAGTGGATTATTCACAGACTGATTTCTCC CAGCTTCTCAACTGCCCTGAATTTGTTCCCCGTCAGCACTACCAAAAGGAGACAGAGTCGGCACCTGGCT CTCCTCGTGCAGTCACCCCAGTGCCAACCAAAACAGAGGAGGTCAGCAACCTAAAGACACTACCCAAGGG CCTGTCTGCCAGCCTGCCTGACCTGGATTCTGAGAACTGGATTGAAGTGAAGAAGAGGCCTCGGCCATCC CCAGCACGGCCCAAGAAGTCAGAGGAGTCCAGATTTTCCCACCTGACCTCCCTGCCTCAGCAGCTGCCTT CCCAGCAGCTGATGTCCAAGGATCAGGATGAGCAAGAGGAACTGGATTTTCTGTTTGACGAGGAGATGGA GCAGATGGATGGGCGGAAGAACACCTTCACTGCCTGGTCTGATGAGGAATCTGACTATGAGATTGATGAC AGGGATGTCAACAAGATCCTCATTGTCACCCAGACACCACATTACATGCGCCGGCACCCAGGGGGGGACC GCACAGGCAACCACACCTCGCGTGCCAAGATGAGCGCCGAACTGGCCAAGGTCATTAATGATGGCCTCTT CTACTATGAGCAGGACCTGTGGGCTGAAAAGTTTGAACCTGAGTATTCCCAGATCAAGCAAGAAGTCGAG AACTTCAAAAAGGTCAATATGATCAGCCGGGAGCAGTTTGACACACTGACCCCTGAGCCCCCTGTGGATC CCAACCAGGAAGTTCCTCCTGGGCCACCTCGGTTCCAGCAAGTTCCTACGGATGCCCTGGCCAACAAGTT GTTTGGTGCTCCTGAGCCCTCCACCATCGCCCGCTCTCTACCAACCACTGTCCCAGAGTCACCAAACTAC CGCAACACCAGGACCCCTCGCACTCCCCGGACACCACAGCTCAAAGACTCAAGCCAGACATCACGGTTTT ACCCAGTGGTGAAAGAAGGACGGACACTGGATGCCAAGATGCCTCGAAAAAGAAAGACAAGACACAGTTC AAACCCACCCTTGGAGAGCCATGTGGGCTGGGTGATGGATTCCCGTGAGCACAGGCCCCGTACTGCTTCC ATCAGCTCCAGCCCCTCAGAAGGGACGCCTACAGTTGGCAGCTATGGCTGTACCCCTCAGTCATTGCCCA AGTTCCAGCATCCTTCCCATGAACTGCTCAAGGAAAATGGCTTCACACAACACGTCTACCATAAGTATCG TAGGCGCTGCCTTAATGAGCGGAAACGCTTGGGCATTGGCCAGTCTCAGGAGATGAACACACTCTTCCGC TTCTGGTCCTTCTTCCTCCGAGATCACTTCAACAAAAAGATGTATGAGGAGTTCAAGCAGCTGGCTCTGG AGGACGCCAAAGAAGGCTACAGATATGGTTTGGAGTGCCTTTTTCGATACTACAGTTATGGCCTGGAAAA GAAGTTCCGGCTGGACATATTCAAGGATTTTCAGGAGGAAACGGTGAAGGACTATGAAGCTGGCCAACTG TATGGGCTGGAGAAGTTCTGGGCCTTCTTGAAATATTCCAAAGCCAAAAATTTGGACATTGACCCCAAAC TGCAAGAATACCTCGGCAAATTCCGACGTCTTGAAGACTTCCGAGTAGATCCCCCCATGGGTGAGGAGGG CAACCACAAGCGACACTCAGTGGTAGCAGGAGGTGGCGGCGGTGAGGGCAGGAAGCGGTGCCCCTCCCAG TCTTCCAGCAGGCCTGCTGCCATGATCAGCCAACCCCCTACACCACCCACCGGCCAGCCTGTCCGGGAAG ATGCCAAATGGACAAGCCAGCACTCGAACACACAGACTTTGGGAAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200935 protein sequence
Red=Cloning site Green=Tags(s) MLWRVLLSKRPPFPHPELDFQEAPIPSCPGRLPGRKNSVALAAAPRKEPTGDREKPLPFPVLAPFSNPEH SAPAKVVRAAVPKQRKGSKVGDFGDAINWPTPGEIAHKSVQPQSHKPQPTRKLPPKKDMKEQEKGEGSDS KESPKTKSDESGEEKNGDEDCQRGGQKKKGNKHKWVPLQIDMKPEVPREKLASRPTRPPEPRHIPANRGE IKGSESATYVPVAPPTPAWQPEIKPEPAWHDQDETSSVKSDGAGGARASFRGRGRGRGRGRGRGRGGTRT HFDYQFGYRKFDGVEGPRTPKYMNNITYYFDNVSSTELYSVDQELLKDYIKRQIEYYFSVDNLERDFFLR RKMDADGFLPITLIASFHRVQALTTDISLIFAALKDSKVVEIVDEKVRRREEPEKWPLPPIVDYSQTDFS QLLNCPEFVPRQHYQKETESAPGSPRAVTPVPTKTEEVSNLKTLPKGLSASLPDLDSENWIEVKKRPRPS PARPKKSEESRFSHLTSLPQQLPSQQLMSKDQDEQEELDFLFDEEMEQMDGRKNTFTAWSDEESDYEIDD RDVNKILIVTQTPHYMRRHPGGDRTGNHTSRAKMSAELAKVINDGLFYYEQDLWAEKFEPEYSQIKQEVE NFKKVNMISREQFDTLTPEPPVDPNQEVPPGPPRFQQVPTDALANKLFGAPEPSTIARSLPTTVPESPNY RNTRTPRTPRTPQLKDSSQTSRFYPVVKEGRTLDAKMPRKRKTRHSSNPPLESHVGWVMDSREHRPRTAS ISSSPSEGTPTVGSYGCTPQSLPKFQHPSHELLKENGFTQHVYHKYRRRCLNERKRLGIGQSQEMNTLFR FWSFFLRDHFNKKMYEEFKQLALEDAKEGYRYGLECLFRYYSYGLEKKFRLDIFKDFQEETVKDYEAGQL YGLEKFWAFLKYSKAKNLDIDPKLQEYLGKFRRLEDFRVDPPMGEEGNHKRHSVVAGGGGGEGRKRCPSQ SSSRPAAMISQPPTPPTGQPVREDAKWTSQHSNTQTLGK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_015315 |
| ORF Size | 3057 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_015315.2 |
| RefSeq Size | 6607 bp |
| RefSeq ORF | 3060 bp |
| Locus ID | 23367 |
| UniProt ID | Q6PKG0 |
| MW | 116.5 kDa |
| Gene Summary | RNA-binding protein that regulates the translation of specific target mRNA species downstream of the mTORC1 complex, in function of growth signals and nutrient availability (PubMed:20430826, PubMed:23711370, PubMed:24532714, PubMed:25940091, PubMed:28650797, PubMed:28673543, PubMed:29244122). Interacts on the one hand with the 3' poly-A tails that are present in all mRNA molecules, and on the other hand with the 7-methylguanosine cap structure of mRNAs containing a 5' terminal oligopyrimidine (5'TOP) motif, which is present in mRNAs encoding ribosomal proteins and several components of the translation machinery (PubMed:23711370, PubMed:25940091, PubMed:28650797, PubMed:29244122, PubMed:26206669, PubMed:28379136). The interaction with the 5' end of mRNAs containing a 5'TOP motif leads to translational repression by preventing the binding of EIF4G1 (PubMed:25940091, PubMed:28650797, PubMed:29244122, PubMed:28379136). When mTORC1 is activated, LARP1 is phosphorylated and dissociates from the 5' untranslated region (UTR) of mRNA (PubMed:25940091, PubMed:28650797). Does not prevent binding of EIF4G1 to mRNAs that lack a 5'TOP motif (PubMed:28379136). Interacts with the free 40S ribosome subunit and with ribosomes, both monosomes and polysomes (PubMed:20430826, PubMed:24532714, PubMed:25940091, PubMed:28673543). Under normal nutrient availability, interacts primarily with the 3' untranslated region (UTR) of mRNAs encoding ribosomal proteins and increases protein synthesis (PubMed:23711370, PubMed:28650797). Associates with actively translating ribosomes and stimulates translation of mRNAs containing a 5'TOP motif, thereby regulating protein synthesis, and as a consequence, cell growth and proliferation (PubMed:20430826, PubMed:24532714). Stabilizes mRNAs species with a 5'TOP motif, which is required to prevent apoptosis (PubMed:20430826, PubMed:23711370, PubMed:25940091, PubMed:28673543).[UniProtKB/Swiss-Prot Function] |
Citations (5)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
An unusual mode of baseline translation adjusts cellular protein synthesis capacity to metabolic needs.
,null,
Cell reports
,PubMed ID 36223745
[LARP1]
|
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mTORC1 promotes TOP mRNA translation through site-specific phosphorylation of LARP1
,Jia, JJ;Lahr, RM;Solgaard, MT;Moraes, BJ;Pointet, R;Yang, AD;Celucci, G;Graber, TE;Hoang, HD;Niklaus, MR;Pena, IA;Hollensen, AK;Smith, EM;Chaker-Margot, M;Anton, L;Dajadian, C;Livingstone, M;Hearnden, J;Wang, XD;Yu, Y;Maier, T;Damgaard, CK;Berman, AJ;Alain, T;Fonseca, BD;,
Nucleic acids research
,PubMed ID 33398329
[LARP1]
|
|
The SARS-CoV-2 RNA-protein interactome in infected human cells
,Schmidt, N;Lareau, CA;Keshishian, H;Ganskih, S;Schneider, C;Hennig, T;Melanson, R;Werner, S;Wei, Y;Zimmer, M;Ade, J;Kirschner, L;Zielinski, S;Dölken, L;Lander, ES;Caliskan, N;Fischer, U;Vogel, J;Carr, SA;Bodem, J;Munschauer, M;,
Nature microbiology
,PubMed ID 33349665
[LARP1]
|
|
LARP1 is a major phosphorylation substrate of mTORC1
,Fonseca, B;Jia, J;Hollensen, A;Pointet, R;Hoang, H;Niklaus, M;Pena, I;Lahr, R;Smith, E;Hearnden, J;Wang, X;Yang, A;Celucci, G;Graber, T;Dajadian, C;Yu, Y;Damgaard, C;Berman, A;Alain, T;,
bioRxiv
[LARP1]
|
|
La-related protein 1 (LARP1) represses terminal oligopyrimidine (TOP) mRNA translation downstream of mTOR complex 1 (mTORC1)
,Fonseca, BD;Zakaria, C;Jia, JJ;Graber, TE;Svitkin, Y;Tahmasebi, S;Healy, D;Hoang, HD;Jensen, JM;Diao, IT;Lussier, A;Dajadian, C;Padmanabhan, N;Wang, W;Matta-Camacho, E;Hearnden, J;Smith, EM;Tsukumo, Y;Yanagiya, A;Morita, M;Petroulakis, E;Gonzalez, JL;Hern,
J. Biol. Chem.
,PubMed ID 25940091
[LARP1]
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Documents
| Product Manuals |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200935L1 | Lenti ORF clone of Human La ribonucleoprotein domain family, member 1 (LARP1), transcript variant 1, Myc-DDK-tagged |
CNY 9,496.00 |
|
| RC200935L2 | Lenti ORF clone of Human La ribonucleoprotein domain family, member 1 (LARP1), transcript variant 1, mGFP tagged |
CNY 10,640.00 |
|
| RC200935L3 | Lenti ORF clone of Human La ribonucleoprotein domain family, member 1 (LARP1), transcript variant 1, Myc-DDK-tagged |
CNY 9,496.00 |
|
| RC200935L4 | Lenti ORF clone of Human La ribonucleoprotein domain family, member 1 (LARP1), transcript variant 1, mGFP tagged |
CNY 9,496.00 |
|
| RG200935 | LARP1 (tGFP-tagged) - Human La ribonucleoprotein domain family, member 1 (LARP1), transcript variant 1 |
CNY 8,696.00 |
|
| SC321557 | LARP1 (untagged)-Human La ribonucleoprotein domain family, member 1 (LARP1), transcript variant 1 |
CNY 7,104.00 |
