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AKAP8L (NM_014371) Human Tagged Lenti ORF Clone
CAT＃: RC200233L3
- LentiORF®
Lenti ORF clone of Human A kinase (PRKA) anchor protein 8-like (AKAP8L), Myc-DDK-tagged
"NM_014371" in other vectors (4)
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CNY 5,890.00
Product images
CNY 600.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HA95; HAP95; NAKAP; NAKAP95 |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data |
The ORF insert of this clone is exactly the same as(RC200233).
|
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NM_014371 |
| ORF Size | 1938 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_014371.2 |
| RefSeq Size | 2231 bp |
| RefSeq ORF | 1941 bp |
| Locus ID | 26993 |
| UniProt ID | Q9ULX6 |
| Domains | AKAP95 |
| Protein Families | Druggable Genome |
| MW | 71.6 kDa |
| Gene Summary | Could play a role in constitutive transport element (CTE)-mediated gene expression by association with DHX9. Increases CTE-dependent nuclear unspliced mRNA export (PubMed:10748171, PubMed:11402034). Proposed to target PRKACA to the nucleus but does not seem to be implicated in the binding of regulatory subunit II of PKA (PubMed:10761695, PubMed:11884601). May be involved in nuclear envelope breakdown and chromatin condensation. May be involved in anchoring nuclear membranes to chromatin in interphase and in releasing membranes from chromating at mitosis (PubMed:11034899). May regulate the initiation phase of DNA replication when associated with TMPO isoform Beta (PubMed:12538639). Required for cell cycle G2/M transition and histone deacetylation during mitosis. In mitotic cells recruits HDAC3 to the vicinity of chromatin leading to deacetylation and subsequent phosphorylation at 'Ser-10' of histone H3; in this function seems to act redundantly with AKAP8 (PubMed:16980585). May be involved in regulation of pre-mRNA splicing (PubMed:17594903).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200233 | AKAP8L (Myc-DDK-tagged)-Human A kinase (PRKA) anchor protein 8-like (AKAP8L) |
CNY 4,808.00 |
|
| RC200233L4 | Lenti ORF clone of Human A kinase (PRKA) anchor protein 8-like (AKAP8L), mGFP tagged |
CNY 5,890.00 |
|
| RG200233 | AKAP8L (tGFP-tagged) - Human A kinase (PRKA) anchor protein 8-like (AKAP8L) |
CNY 6,408.00 |
|
| SC321388 | AKAP8L (untagged)-Human A kinase (PRKA) anchor protein 8-like (AKAP8L) |
CNY 4,816.00 |
