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Flotillin 1 (FLOT1) (NM_005803) Human Tagged ORF Clone
CAT＃: RC200231
FLOT1 (Myc-DDK-tagged)-Human flotillin 1 (FLOT1)
ORF Plasmid: tGFP
"NM_005803" in other vectors (6)
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CNY 3,656.00
CNY 5,320.00
| Cited in 2 publications. |
Product images
CNY 300.00
热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC200231 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTTTCACTTGTGGCCCAAATGAGGCCATGGTGGTCTCCGGGTTCTGCCGAAGCCCCCCAGTCATGG TGGCTGGAGGGCGTGTCTTTGTCCTGCCCTGCATCCAACAGATCCAGAGGATCTCTCTCAACACACTGAC CCTCAATGTCAAGAGTGAAAAGGTTTACACTCGCCATGGGGTCCCCATCTCAGTCACTGGCATTGCCCAG GTAAAAATCCAGGGGCAGAACAAGGAGATGTTGGCGGCCGCCTGTCAGATGTTCCTGGGGAAGACGGAGG CTGAGATTGCCCACATTGCCCTGGAGACGTTAGAGGGCCACCAGAGGGCCATCATGGCCCACATGACTGT GGAGGAGATCTATAAGGACAGGCAGAAATTCTCAGAACAGGTTTTCAAAGTGGCCTCCTCAGACCTGGTC AACATGGGCATCAGTGTGGTTAGCTACACTCTGAAGGACATTCACGATGACCAGGACTATTTGCACTCTT TGGGGAAGGCTCGAACAGCTCAAGTCCAAAAAGATGCACGGATTGGAGAAGCAGAGGCCAAGAGAGATGC TGGGATCCGGGAAGCTAAAGCCAAGCAGGAAAAGGTGTCTGCTCAGTACCTGAGTGAGATCGAGATGGCC AAGGCACAGAGAGATTACGAACTGAAGAAGGCCGCCTATGACATCGAGGTCAACACCCGCCGAGCACAGG CTGACCTGGCCTATCAGCTTCAGGTGGCCAAGACTAAGCAGCAGATTGAGGAGCAGCGGGTGCAGGTGCA GGTGGTGGAGCGGGCCCAGCAGGTGGCAGTGCAGGAGCAGGAGATCGCCCGGCGGGAGAAGGAGCTGGAG GCCCGGGTGCGGAAGCCAGCGGAAGCGGAGCGCTACAAGCTGGAGCGCCTAGCCGAGGCAGAGAAGTCCC AACTAATTATGCAGGCGGAGGCAGAAGCCGCGTCTGTGCGGATGCGTGGGGAAGCTGAGGCCTTTGCCAT AGGGGCCCGAGCCCGAGCCGAGGCTGAGCAGATGGCCAAGAAGGCAGAAGCCTTCCAGCTGTACCAAGAG GCTGCTCAGCTGGACATGCTGCTAGAGAAGCTGCCCCAGGTGGCAGAGGAGATCAGTGGTCCCTTGACTT CAGCCAATAAGATCACACTGGTGTCCAGCGGCAGTGGGACCATGGGGGCAGCCAAAGTGACTGGGGAAGT ACTGGACATTCTAACTCGCCTGCCAGAGAGTGTGGAAAGACTCACAGGCGTGAGCATCTCCCAGGTGAAT CACAAGCCTTTGAGAACAGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200231 protein sequence
Red=Cloning site Green=Tags(s) MFFTCGPNEAMVVSGFCRSPPVMVAGGRVFVLPCIQQIQRISLNTLTLNVKSEKVYTRHGVPISVTGIAQ VKIQGQNKEMLAAACQMFLGKTEAEIAHIALETLEGHQRAIMAHMTVEEIYKDRQKFSEQVFKVASSDLV NMGISVVSYTLKDIHDDQDYLHSLGKARTAQVQKDARIGEAEAKRDAGIREAKAKQEKVSAQYLSEIEMA KAQRDYELKKAAYDIEVNTRRAQADLAYQLQVAKTKQQIEEQRVQVQVVERAQQVAVQEQEIARREKELE ARVRKPAEAERYKLERLAEAEKSQLIMQAEAEAASVRMRGEAEAFAIGARARAEAEQMAKKAEAFQLYQE AAQLDMLLEKLPQVAEEISGPLTSANKITLVSSGSGTMGAAKVTGEVLDILTRLPESVERLTGVSISQVN HKPLRTA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005803 |
| ORF Size | 1281 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005803.4 |
| RefSeq Size | 1839 bp |
| RefSeq ORF | 1284 bp |
| Locus ID | 10211 |
| UniProt ID | O75955 |
| Domains | Band_7, Flotillin |
| Protein Pathways | Insulin signaling pathway |
| MW | 47.4 kDa |
| Gene Summary | This gene encodes an protein that localizes to the caveolae, which are small domains on the inner cell membranes. This protein plays a role in vesicle trafficking and cell morphology. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Flotillin-2 regulates epidermal growth factor receptor activation, degradation by Cbl-mediated ubiquitination, and cancer growth.
,null,
The Journal of biological chemistry
,PubMed ID 36470425
[Flotillin 1]
|
|
MAL2 mediates the formation of stable HER2 signaling complexes within lipid raft-rich membrane protrusions in breast cancer cells
,null,
Cell reports
,PubMed ID 34965434
[Flotillin 1]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200231L1 | Lenti ORF clone of Human flotillin 1 (FLOT1), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC200231L2 | Lenti ORF clone of Human flotillin 1 (FLOT1), mGFP tagged |
CNY 5,890.00 |
|
| RC200231L3 | Lenti ORF clone of Human flotillin 1 (FLOT1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC200231L4 | Lenti ORF clone of Human flotillin 1 (FLOT1), mGFP tagged |
CNY 6,056.00 |
|
| RG200231 | FLOT1 (tGFP-tagged) - Human flotillin 1 (FLOT1) |
CNY 5,256.00 |
|
| SC319463 | FLOT1 (untagged)-Human flotillin 1 (FLOT1) |
CNY 3,656.00 |
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