Zswim8 (NM_001252081) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	2310021P13Rik; 4832404P21Rik; Kiaa0913; mKIAA0913
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231882 representing NM_001252081 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTGATGTTCGCGGAGTGGGAGGACGGCGAGCGCTTCTCGTTTGAGGATTCCGACCGCTTTGAGG AGGATTCACTCTGTTCGTTCATTTCCGAGGCCGAGAGCCTTTGCCAGAACTGGCGAGGATGGCGCAAACA GTCAGCGGGGCCCAATTCCCCCACTGGAGGCGGTGGCGGAGGTGGCAGTGGCGGTACCAGAACGCGAGAT GGATTGGTAATCCCATTGGTGGAGCTGTCAGCAAAGCAGGTGGCATTTCACATCCCATTTGAAGTGGTAG AGAAAGTTTATCCTCCAGTGCCAGAACAACTCCAACTCCGAATTGCTTTTTGGAGCTTCCCTGAGAATGA AGAGGACATTCGTCTGTATTCATGCCTAGCCAATGGCAGTGCGGATGAGTTTCAGCGAGGGGATCAGCTG TTCCGAATGAGGGCTGTGAAAGACCCGCTGCAGATAGGGTTCCATCTGAGCGCCACAGTGGTACCACCGC AAATGGTCCCACCCAAGGGGGCCTACAATGTAGCTGTGATGTTTGACCGCTGCCGGGTCACTTCTTGTAG CTGTACCTGTGGGGCCGGGGCCAAATGGTGCACCCATGTCGTGGCACTCTGCCTCTTCCGCATTCACAAC GCATCTGCAGTCTGCCTGCGGGCTCCAGTCTCAGAGTCCCTGTCTCGGCTACAAAGGGACCAGCTTCAAA AATTTGCTCAGTACCTTATCAGTGAGCTTCCTCAGCAGATTCTCCCCACAGCCCAGCGTCTTCTAGACGA GCTCCTTTCCTCCCAGTCCACAGCCATCAACACAGTGTGTGGGGCTCCGGACCCTACAGCAGGGCCCTCA GCTTCAGACCAGAGCACTTGGTATTTGGATGAGTCAACACTCACTGACAACATAAAGAAGACACTACATA AGTTCTGTGGCCCCTCCCCTGTGGTCTTCAGTGATGTAAACTCTATGTATCTCTCTTCCACGGAACCTCC TGCTGCTGCTGAGTGGGCATGTCTGCTGCGGCCTCTGAGGGGTCGAGAGCCTGAGGGTGTCTGGAACCTG CTTAGCATTGTTCGAGAGATGTTCAAGCGAAGGGACAGCAATGCTGCCCCCTTGCTGGAAATACTCACTG ACCAGTGCCTCACCTATGAACAGATAACAGGCTGGTGGTATAGTGTGCGCACCTCAGCTTCACACAGCAG TGCCAGTGGTCACACAGGCCGTAGCAATGGGCAGTCAGAGGTAGCAGCCCATGCATGTGCAAGTATGTGC GACGAGATGGTTACCCTCTGGAGGCTGGCTGTGCTGGACCCTGCCCTCAGCCCTCAGCGCCGCCGGGAAC TGTGTGCACAGCTGCGTCAGTGGCAACTGAAGGTGATTGAGAATGTCAAGCGGGGCCAGCACAAGAAGAC CCTAGAGAGGCTCTTCCCTGGCTTCCGGCCAGCTGTGGAGGCCTGCTACTTTAACTGGGAAGAGGCTTAT CCTCTTCCTGGTGTTACCTACAGTGGCACTGACCGGAAGCTAGCCCTGTGCTGGGCCCGGGCCCTGCCTG CCAGGCCAGGAGCCTCTAGATCTGGGGGCCTGGAAGAGTCCCGGCCCCGACCTCTTCCTACTGAGCCAGC TGTGAGGCCCAAGGAACCTGGGGCCAAACGCAAAGGATTGGGTGAGGGGATCTCCTCACAGCGGGGCCCC CGCCGCCTCTCTGCCGAAGGAGGAGATAAGGCTCTGCATAAGATGGGTCCAAGTGGGGGCAAAGCCAAGG TACTGGGTGGGACTGGCAGCGGGGGCAAGAGCTCAGCAGGCAGTGGGAGCAAACGGCGGCTAAGCAGTGA AGACAGCTCCCTGGAACCAGACCTGGCAGAGATGAGCCTGGATGACAGCAGCCTGGCCCTGGGTGCAGAG GCCAGCACCTTTGGTGGATTCCCTGAGAGCCCTCCACCCTGTCCTTCCTCGGTTGGCTCCAGAGGACCTT CCACCTTCCTTCCTGAGCCCCCAGATACTTATGAGGAAGATGCTGGTGTGTACTTTTCAGAAGGACCTGA GCCTCCCACAGCCTCTGCAGACCACCCTGGTCTGCTGCCTGGGGAGGTCTGTACCCGAGATGACCTCCCT TCCACAGACGACAGTGGCAGTGGGCTACACAAAACCAAAGAAGCAGCTCCTGCAGTTGGAGAGGAGGATG ACGACTACCAAGCATATTACCTGAATGCCCAGGATGGGGCTGGAGGCGAGGAGGAGAAGGCGGAGGGCGG GACTGGGGAGGAGCATGACCTGTTTGCTGGTTTGAAGCCACTGGAACAGGAGAGCCGAATGGAGGTGTTG TTTGCCTGCGCTGAGGCCCTGCATGCCCATGGCTACAGCAATGAGGCCTCCCGCCTCACTGTGGAGCTTG CCCAGGACCTGCTAGCCAACCCACCTGACCTCAAGGGCAAGAAAAACAAGGTGTCCACAAGCCGTCAGAC CTGGGTGGCTACCAACACTCTGACCAAAGCAGCCTTCCTGTTGACAGTGCTTAGTGAGCGCCCTGAGCAC CACAGCCTGGCCTTCCGAGTTGGCATGTTTGCGCTGGAGCTACAGCGGCCTCCTGCTTCAACCAAGGCCT TGGAGGTAAAATTGGCATATCAGGAATCTGAGGTAGCTGCTCTGCTCAAGAAGATCCCTCGGGGCCCCAG TGAAATGAGTACCATTCGATGCCGGGCAGAAGAACTTCGGGAGGGTACACTGTGTGACTATCGGCCTGTC TTGCCCCTCATGTTGGCCAGTTTCATCTTTGATGTTCTCTGTGCTCCAGTGGTTTCTCTCACGGGTTCCC GGCCCCCAAGTCGAAACTGGACTAACGAGATGCCTGGAGATGAGGAGCTAGGATTTGAAGCAGCAGTTGC TGCCTTGGGCATGAAGACAACAGTGAGTGAGGCAGAACATCCCCTCCTATGTGAAGGCACACGTCGGGAG AAGGGTGACCTGGCCTTAGCACTCATGATCACCTACAAGGATGACCAGGCCAAGCTCAAGAAGATCCTAG ACAAACTCTTGGACCGAGAAAGCCAGACACATAAGCCACAGACCCTGAGTTCTTTCTACTCATCCAGCCG GCCGGCCACAGCCAACCAGAGATCTCCTTCAAAGCACGGGGCCCCATCTGCTCCCGGGGCCCTGCAGCCA CTGACTTCAAGCTCTGCAGGGCCTGCTCAGCCAGGGAATGTGGCAGGGGCTGGGCCAGGTCCCACTGAGG GCTTCACAGAGAAGAATGTACCCGAAAGTTCCCCACATTCCCCCTGTGAAGGTCTCCCACCTGAGGCAGC TTTGACTCCACGGCCGGAGGGGAAGGTTCCTAGCCGCCTAGCACTTGGCAGTCGTGGAGGTTATAATGGT CGAGGTTGGGGCTCTCCAGGGCGGCCCAAAAAGAAACACACAGGCATGGCCAGCATTGACAGCAGTGCCC CTGAAACCACATCGGACAGCTCTCCTACCTTAAGCCGACGGCCACTTCGAGGGGGCTGGGCCCCTACTTC TTGGGGTCGAGGACAAGACAGTGACAGCATTAGCAGCTCTTCCTCAGACTCTCTGGGCTCTTCATCCTCC AGTGGAAGCCGCCGGGCTAGTGCCAGTGGAGGGGCCCGGGCAAAGACAGTTGACGTTGGCAGGTGTTACA AAGGCCGCCGCCCTGAGAGTCATGCCCCCCACGTACCCAATCAGCCGTCAGAGGCAGCTGCACACTTCTA CTTCGAATTGGCGAAGACAGTTCTGATCAAGGCAGGGGGCAACAGCAGCACCTCCATTTTCACACATCCA TCTTCCTCAGGAGGCCACCAGGGTCCTCACCGCAACCTGCACCTTTGCGCCTTTGAGATTGGGCTTTATG CCCTAGGCCTGCATAACTTTGTTTCTCCTAACTGGCTCTCTCGTACCTATTCTTCCCATGTATCCTGGAT TACAGGGCAGGCCATGGAGATTGGCAGTGCAGCCTTGACTATACTGGTAGAATGCTGGGATGGGCACCTG ACACCCCCTGAGGTTGCATCGCTGGCCGACAGAGCGTCACGGGCACGAGACTCCAACATGGTGAGGGCAG CGGCAGAGCTGGCTCTAAGCTGCCTGCCTCATGCCCACGCACTGAACCCCAATGAGATTCAGCGGGCCTT GGTGCAGTGCAAGGAACAGGATAACCTGATGTTGGAGAAGGCCTGCATGGCCGTGGAAGAGGCAGCCAAG GGTGGGGGCGTATACCCTGAAGTGCTGTTTGAGGTTGCTCATCAGTGGTTCTGGCTTTATGAGGAGACAG CAGGCGGCTCGTCCACAGCTCGTGAAGGGGCTACAAGCTGTAGTGGCAGTGGGATGAGGGCCGCTGGGGA GGCTGGGCGGGGACTCCCTGAGGGTAGGGGTGCCCCAGGGACTGAACCTGTTACAGTGGCTGCGGCAGCA GTGACAGCAGCAGCCACAGTGGTTCCGGTCATCTCAGTGGGGTCCAGTTTATATCCAGGTCCAGGACTGG GGCATGGCCACTCCCCTGGCCTGCACCCCTACACTGCTCTCCAGCCCCACTTGCCCTGCAGCCCTCAGTA CCTCACCCACCCAGCTCACCCTGCCCACCCTATGCCTCATATGCCCCGGCCTGCCGTCTTCCCTGTGCCC AGCTCTGCATACCCACAGGGTGTGCATCCTGCATTCCTGGGGGCGCAATACCCTTACTCAGTGACTCCTC CCTCGCTTGCTGCCACAGCTGTATCTTTCCCTGTCCCTTCCATGGCTCCCATCACAGTCCATCCTTACCA CACAGAACCAGGGCTCCCACTGCCCACCAGTGTGGCCTTGAGCAGTGTCCATCCAGCATCTACGTTTCCA GCCATCCAGGGTGCCTCACTGCCTGCTCTGACCACACAGCCCAGCCCTCTGGTAAGCGGGGGTTTTCCAC CACCCGAAGAGGAGACACACAGTCAACCGGTCAATCCACATAGCCTGCACCATCTGCATGCTGCTTACCG TGTTGGGATGCTGGCACTGGAGATGCTAGGTCGCCGGGCACACAACGATCACCCCAACAACTTTTCCCGC TCCCCCCCTTACACTGATGATGTCAAATGGTTGCTGGGGCTGGCAGCAAAGCTGGGAGTGAACTACGTGC ACCAGTTCTGTGTGGGGGCAGCCAAGGGGGTGCTGAGCCCGTTTGTGCTGCAGGAGATCGTCATGGAGAC GCTGCAGCGGCTGAACCCCATTCATGCCCACAACCACCTTCGAGCCCCGGCCTTCCACCAACTGGTGCAG CGCTGTCAGCAGGCATACATGCAGTACATCCATCACCGCTTAATTCACCTGACTCCTGCCGACTACGACG ACTTTGTGAATGCAATCCGCAGCGCTCGCAGCGCCTTCTGCCTGACACCCATGGGCATGATGCAGTTCAA CGACATCCTACAGAACCTCAAACGCAGCAAACAGACCAAGGAGTTGTGGCAGCGGGTCTCTCTGGAGATA ACCACCTTCTCCCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231882 representing NM_001252081 Red=Cloning site Green=Tags(s) MELMFAEWEDGERFSFEDSDRFEEDSLCSFISEAESLCQNWRGWRKQSAGPNSPTGGGGGGGSGGTRTRD GLVIPLVELSAKQVAFHIPFEVVEKVYPPVPEQLQLRIAFWSFPENEEDIRLYSCLANGSADEFQRGDQL FRMRAVKDPLQIGFHLSATVVPPQMVPPKGAYNVAVMFDRCRVTSCSCTCGAGAKWCTHVVALCLFRIHN ASAVCLRAPVSESLSRLQRDQLQKFAQYLISELPQQILPTAQRLLDELLSSQSTAINTVCGAPDPTAGPS ASDQSTWYLDESTLTDNIKKTLHKFCGPSPVVFSDVNSMYLSSTEPPAAAEWACLLRPLRGREPEGVWNL LSIVREMFKRRDSNAAPLLEILTDQCLTYEQITGWWYSVRTSASHSSASGHTGRSNGQSEVAAHACASMC DEMVTLWRLAVLDPALSPQRRRELCAQLRQWQLKVIENVKRGQHKKTLERLFPGFRPAVEACYFNWEEAY PLPGVTYSGTDRKLALCWARALPARPGASRSGGLEESRPRPLPTEPAVRPKEPGAKRKGLGEGISSQRGP RRLSAEGGDKALHKMGPSGGKAKVLGGTGSGGKSSAGSGSKRRLSSEDSSLEPDLAEMSLDDSSLALGAE ASTFGGFPESPPPCPSSVGSRGPSTFLPEPPDTYEEDAGVYFSEGPEPPTASADHPGLLPGEVCTRDDLP STDDSGSGLHKTKEAAPAVGEEDDDYQAYYLNAQDGAGGEEEKAEGGTGEEHDLFAGLKPLEQESRMEVL FACAEALHAHGYSNEASRLTVELAQDLLANPPDLKGKKNKVSTSRQTWVATNTLTKAAFLLTVLSERPEH HSLAFRVGMFALELQRPPASTKALEVKLAYQESEVAALLKKIPRGPSEMSTIRCRAEELREGTLCDYRPV LPLMLASFIFDVLCAPVVSLTGSRPPSRNWTNEMPGDEELGFEAAVAALGMKTTVSEAEHPLLCEGTRRE KGDLALALMITYKDDQAKLKKILDKLLDRESQTHKPQTLSSFYSSSRPATANQRSPSKHGAPSAPGALQP LTSSSAGPAQPGNVAGAGPGPTEGFTEKNVPESSPHSPCEGLPPEAALTPRPEGKVPSRLALGSRGGYNG RGWGSPGRPKKKHTGMASIDSSAPETTSDSSPTLSRRPLRGGWAPTSWGRGQDSDSISSSSSDSLGSSSS SGSRRASASGGARAKTVDVGRCYKGRRPESHAPHVPNQPSEAAAHFYFELAKTVLIKAGGNSSTSIFTHP SSSGGHQGPHRNLHLCAFEIGLYALGLHNFVSPNWLSRTYSSHVSWITGQAMEIGSAALTILVECWDGHL TPPEVASLADRASRARDSNMVRAAAELALSCLPHAHALNPNEIQRALVQCKEQDNLMLEKACMAVEEAAK GGGVYPEVLFEVAHQWFWLYEETAGGSSTAREGATSCSGSGMRAAGEAGRGLPEGRGAPGTEPVTVAAAA VTAAATVVPVISVGSSLYPGPGLGHGHSPGLHPYTALQPHLPCSPQYLTHPAHPAHPMPHMPRPAVFPVP SSAYPQGVHPAFLGAQYPYSVTPPSLAATAVSFPVPSMAPITVHPYHTEPGLPLPTSVALSSVHPASTFP AIQGASLPALTTQPSPLVSGGFPPPEEETHSQPVNPHSLHHLHAAYRVGMLALEMLGRRAHNDHPNNFSR SPPYTDDVKWLLGLAAKLGVNYVHQFCVGAAKGVLSPFVLQEIVMETLQRLNPIHAHNHLRAPAFHQLVQ RCQQAYMQYIHHRLIHLTPADYDDFVNAIRSARSAFCLTPMGMMQFNDILQNLKRSKQTKELWQRVSLEI TTFSP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001252081
ORF Size	5475 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001252081.1, NP_001239010.1
RefSeq Size	6073 bp
RefSeq ORF	5478 bp
Locus ID	268721
UniProt ID	Q3UHH1
MW	196.8 kDa
Gene Summary	Substrate recognition component of a SCF-like E3 ubiquitin-protein ligase complex that promotes target-directed microRNA degradation (TDMD), a process that mediates degradation of microRNAs (miRNAs) (PubMed:33184237). The SCF-like E3 ubiquitin-protein ligase complex acts by catalyzing ubiquitination and subsequent degradation of AGO proteins (AGO1, AGO2, AGO3 and/or AGO4), thereby exposing miRNAs for degradation (By similarity). Specifically recognizes and binds AGO proteins when they are engaged with a TDMD target (By similarity). May also acts as a regulator of axon guidance: specifically recognizes misfolded ROBO3 and promotes its ubiquitination and subsequent degradation (By similarity).[UniProtKB/Swiss-Prot Function]

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