Zswim8 (NM_001252081) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2310021P13Rik; 4832404P21Rik; Kiaa0913; mKIAA0913
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229671 representing NM_001252081 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTGATGTTCGCGGAGTGGGAGGACGGCGAGCGCTTCTCGTTTGAGGATTCCGACCGCTTTGAGG AGGATTCACTCTGTTCGTTCATTTCCGAGGCCGAGAGCCTTTGCCAGAACTGGCGAGGATGGCGCAAACA GTCAGCGGGGCCCAATTCCCCCACTGGAGGCGGTGGCGGAGGTGGCAGTGGCGGTACCAGAACGCGAGAT GGATTGGTAATCCCATTGGTGGAGCTGTCAGCAAAGCAGGTGGCATTTCACATCCCATTTGAAGTGGTAG AGAAAGTTTATCCTCCAGTGCCAGAACAACTCCAACTCCGAATTGCTTTTTGGAGCTTCCCTGAGAATGA AGAGGACATTCGTCTGTATTCATGCCTAGCCAATGGCAGTGCGGATGAGTTTCAGCGAGGGGATCAGCTG TTCCGAATGAGGGCTGTGAAAGACCCGCTGCAGATAGGGTTCCATCTGAGCGCCACAGTGGTACCACCGC AAATGGTCCCACCCAAGGGGGCCTACAATGTAGCTGTGATGTTTGACCGCTGCCGGGTCACTTCTTGTAG CTGTACCTGTGGGGCCGGGGCCAAATGGTGCACCCATGTCGTGGCACTCTGCCTCTTCCGCATTCACAAC GCATCTGCAGTCTGCCTGCGGGCTCCAGTCTCAGAGTCCCTGTCTCGGCTACAAAGGGACCAGCTTCAAA AATTTGCTCAGTACCTTATCAGTGAGCTTCCTCAGCAGATTCTCCCCACAGCCCAGCGTCTTCTAGACGA GCTCCTTTCCTCCCAGTCCACAGCCATCAACACAGTGTGTGGGGCTCCGGACCCTACAGCAGGGCCCTCA GCTTCAGACCAGAGCACTTGGTATTTGGATGAGTCAACACTCACTGACAACATAAAGAAGACACTACATA AGTTCTGTGGCCCCTCCCCTGTGGTCTTCAGTGATGTAAACTCTATGTATCTCTCTTCCACGGAACCTCC TGCTGCTGCTGAGTGGGCATGTCTGCTGCGGCCTCTGAGGGGTCGAGAGCCTGAGGGTGTCTGGAACCTG CTTAGCATTGTTCGAGAGATGTTCAAGCGAAGGGACAGCAATGCTGCCCCCTTGCTGGAAATACTCACTG ACCAGTGCCTCACCTATGAACAGATAACAGGCTGGTGGTATAGTGTGCGCACCTCAGCTTCACACAGCAG TGCCAGTGGTCACACAGGCCGTAGCAATGGGCAGTCAGAGGTAGCAGCCCATGCATGTGCAAGTATGTGC GACGAGATGGTTACCCTCTGGAGGCTGGCTGTGCTGGACCCTGCCCTCAGCCCTCAGCGCCGCCGGGAAC TGTGTGCACAGCTGCGTCAGTGGCAACTGAAGGTGATTGAGAATGTCAAGCGGGGCCAGCACAAGAAGAC CCTAGAGAGGCTCTTCCCTGGCTTCCGGCCAGCTGTGGAGGCCTGCTACTTTAACTGGGAAGAGGCTTAT CCTCTTCCTGGTGTTACCTACAGTGGCACTGACCGGAAGCTAGCCCTGTGCTGGGCCCGGGCCCTGCCTG CCAGGCCAGGAGCCTCTAGATCTGGGGGCCTGGAAGAGTCCCGGCCCCGACCTCTTCCTACTGAGCCAGC TGTGAGGCCCAAGGAACCTGGGGCCAAACGCAAAGGATTGGGTGAGGGGATCTCCTCACAGCGGGGCCCC CGCCGCCTCTCTGCCGAAGGAGGAGATAAGGCTCTGCATAAGATGGGTCCAAGTGGGGGCAAAGCCAAGG TACTGGGTGGGACTGGCAGCGGGGGCAAGAGCTCAGCAGGCAGTGGGAGCAAACGGCGGCTAAGCAGTGA AGACAGCTCCCTGGAACCAGACCTGGCAGAGATGAGCCTGGATGACAGCAGCCTGGCCCTGGGTGCAGAG GCCAGCACCTTTGGTGGATTCCCTGAGAGCCCTCCACCCTGTCCTTCCTCGGTTGGCTCCAGAGGACCTT CCACCTTCCTTCCTGAGCCCCCAGATACTTATGAGGAAGATGCTGGTGTGTACTTTTCAGAAGGACCTGA GCCTCCCACAGCCTCTGCAGACCACCCTGGTCTGCTGCCTGGGGAGGTCTGTACCCGAGATGACCTCCCT TCCACAGACGACAGTGGCAGTGGGCTACACAAAACCAAAGAAGCAGCTCCTGCAGTTGGAGAGGAGGATG ACGACTACCAAGCATATTACCTGAATGCCCAGGATGGGGCTGGAGGCGAGGAGGAGAAGGCGGAGGGCGG GACTGGGGAGGAGCATGACCTGTTTGCTGGTTTGAAGCCACTGGAACAGGAGAGCCGAATGGAGGTGTTG TTTGCCTGCGCTGAGGCCCTGCATGCCCATGGCTACAGCAATGAGGCCTCCCGCCTCACTGTGGAGCTTG CCCAGGACCTGCTAGCCAACCCACCTGACCTCAAGGGCAAGAAAAACAAGGTGTCCACAAGCCGTCAGAC CTGGGTGGCTACCAACACTCTGACCAAAGCAGCCTTCCTGTTGACAGTGCTTAGTGAGCGCCCTGAGCAC CACAGCCTGGCCTTCCGAGTTGGCATGTTTGCGCTGGAGCTACAGCGGCCTCCTGCTTCAACCAAGGCCT TGGAGGTAAAATTGGCATATCAGGAATCTGAGGTAGCTGCTCTGCTCAAGAAGATCCCTCGGGGCCCCAG TGAAATGAGTACCATTCGATGCCGGGCAGAAGAACTTCGGGAGGGTACACTGTGTGACTATCGGCCTGTC TTGCCCCTCATGTTGGCCAGTTTCATCTTTGATGTTCTCTGTGCTCCAGTGGTTTCTCTCACGGGTTCCC GGCCCCCAAGTCGAAACTGGACTAACGAGATGCCTGGAGATGAGGAGCTAGGATTTGAAGCAGCAGTTGC TGCCTTGGGCATGAAGACAACAGTGAGTGAGGCAGAACATCCCCTCCTATGTGAAGGCACACGTCGGGAG AAGGGTGACCTGGCCTTAGCACTCATGATCACCTACAAGGATGACCAGGCCAAGCTCAAGAAGATCCTAG ACAAACTCTTGGACCGAGAAAGCCAGACACATAAGCCACAGACCCTGAGTTCTTTCTACTCATCCAGCCG GCCGGCCACAGCCAACCAGAGATCTCCTTCAAAGCACGGGGCCCCATCTGCTCCCGGGGCCCTGCAGCCA CTGACTTCAAGCTCTGCAGGGCCTGCTCAGCCAGGGAATGTGGCAGGGGCTGGGCCAGGTCCCACTGAGG GCTTCACAGAGAAGAATGTACCCGAAAGTTCCCCACATTCCCCCTGTGAAGGTCTCCCACCTGAGGCAGC TTTGACTCCACGGCCGGAGGGGAAGGTTCCTAGCCGCCTAGCACTTGGCAGTCGTGGAGGTTATAATGGT CGAGGTTGGGGCTCTCCAGGGCGGCCCAAAAAGAAACACACAGGCATGGCCAGCATTGACAGCAGTGCCC CTGAAACCACATCGGACAGCTCTCCTACCTTAAGCCGACGGCCACTTCGAGGGGGCTGGGCCCCTACTTC TTGGGGTCGAGGACAAGACAGTGACAGCATTAGCAGCTCTTCCTCAGACTCTCTGGGCTCTTCATCCTCC AGTGGAAGCCGCCGGGCTAGTGCCAGTGGAGGGGCCCGGGCAAAGACAGTTGACGTTGGCAGGTGTTACA AAGGCCGCCGCCCTGAGAGTCATGCCCCCCACGTACCCAATCAGCCGTCAGAGGCAGCTGCACACTTCTA CTTCGAATTGGCGAAGACAGTTCTGATCAAGGCAGGGGGCAACAGCAGCACCTCCATTTTCACACATCCA TCTTCCTCAGGAGGCCACCAGGGTCCTCACCGCAACCTGCACCTTTGCGCCTTTGAGATTGGGCTTTATG CCCTAGGCCTGCATAACTTTGTTTCTCCTAACTGGCTCTCTCGTACCTATTCTTCCCATGTATCCTGGAT TACAGGGCAGGCCATGGAGATTGGCAGTGCAGCCTTGACTATACTGGTAGAATGCTGGGATGGGCACCTG ACACCCCCTGAGGTTGCATCGCTGGCCGACAGAGCGTCACGGGCACGAGACTCCAACATGGTGAGGGCAG CGGCAGAGCTGGCTCTAAGCTGCCTGCCTCATGCCCACGCACTGAACCCCAATGAGATTCAGCGGGCCTT GGTGCAGTGCAAGGAACAGGATAACCTGATGTTGGAGAAGGCCTGCATGGCCGTGGAAGAGGCAGCCAAG GGTGGGGGCGTATACCCTGAAGTGCTGTTTGAGGTTGCTCATCAGTGGTTCTGGCTTTATGAGGAGACAG CAGGCGGCTCGTCCACAGCTCGTGAAGGGGCTACAAGCTGTAGTGGCAGTGGGATGAGGGCCGCTGGGGA GGCTGGGCGGGGACTCCCTGAGGGTAGGGGTGCCCCAGGGACTGAACCTGTTACAGTGGCTGCGGCAGCA GTGACAGCAGCAGCCACAGTGGTTCCGGTCATCTCAGTGGGGTCCAGTTTATATCCAGGTCCAGGACTGG GGCATGGCCACTCCCCTGGCCTGCACCCCTACACTGCTCTCCAGCCCCACTTGCCCTGCAGCCCTCAGTA CCTCACCCACCCAGCTCACCCTGCCCACCCTATGCCTCATATGCCCCGGCCTGCCGTCTTCCCTGTGCCC AGCTCTGCATACCCACAGGGTGTGCATCCTGCATTCCTGGGGGCGCAATACCCTTACTCAGTGACTCCTC CCTCGCTTGCTGCCACAGCTGTATCTTTCCCTGTCCCTTCCATGGCTCCCATCACAGTCCATCCTTACCA CACAGAACCAGGGCTCCCACTGCCCACCAGTGTGGCCTTGAGCAGTGTCCATCCAGCATCTACGTTTCCA GCCATCCAGGGTGCCTCACTGCCTGCTCTGACCACACAGCCCAGCCCTCTGGTAAGCGGGGGTTTTCCAC CACCCGAAGAGGAGACACACAGTCAACCGGTCAATCCACATAGCCTGCACCATCTGCATGCTGCTTACCG TGTTGGGATGCTGGCACTGGAGATGCTAGGTCGCCGGGCACACAACGATCACCCCAACAACTTTTCCCGC TCCCCCCCTTACACTGATGATGTCAAATGGTTGCTGGGGCTGGCAGCAAAGCTGGGAGTGAACTACGTGC ACCAGTTCTGTGTGGGGGCAGCCAAGGGGGTGCTGAGCCCGTTTGTGCTGCAGGAGATCGTCATGGAGAC GCTGCAGCGGCTGAACCCCATTCATGCCCACAACCACCTTCGAGCCCCGGCCTTCCACCAACTGGTGCAG CGCTGTCAGCAGGCATACATGCAGTACATCCATCACCGCTTAATTCACCTGACTCCTGCCGACTACGACG ACTTTGTGAATGCAATCCGCAGCGCTCGCAGCGCCTTCTGCCTGACACCCATGGGCATGATGCAGTTCAA CGACATCCTACAGAACCTCAAACGCAGCAAACAGACCAAGGAGTTGTGGCAGCGGGTCTCTCTGGAGATA ACCACCTTCTCCCCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001252081
Insert Size	5478 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001252081.1, NP_001239010.1
RefSeq Size	6073 bp
RefSeq ORF	5478 bp
Locus ID	268721
UniProt ID	Q3UHH1
Gene Summary	Substrate recognition component of a SCF-like E3 ubiquitin-protein ligase complex that promotes target-directed microRNA degradation (TDMD), a process that mediates degradation of microRNAs (miRNAs) (PubMed:33184237). The SCF-like E3 ubiquitin-protein ligase complex acts by catalyzing ubiquitination and subsequent degradation of AGO proteins (AGO1, AGO2, AGO3 and/or AGO4), thereby exposing miRNAs for degradation (By similarity). Specifically recognizes and binds AGO proteins when they are engaged with a TDMD target (By similarity). May also acts as a regulator of axon guidance: specifically recognizes misfolded ROBO3 and promotes its ubiquitination and subsequent degradation (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region, compared to variant 1, resulting in an isoform (2) that is shorter than isoform 1.

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