Myo18a (NM_001291215) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	MAJN; MyoPDZ; MysPDZ; SP-R210
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231868 representing NM_001291215 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCTGGACCCCGAGGCCGCCTCTCCTGCCTACAGCCAGGCCAAGACGGAAGAGCAGATTGCCGCTG AAGAGGCCTGGTATGAGACGGAGAAGGTGTGGCTGGTCCATAGAGATGGATTCTCCTTAGCCAGCCAGCT CAAGTCTGAGGAGCTCAGCCTACCCGAGGGGAAGGCGCGTGTGAAGCTGGACCATGACGGAGCCATCCTG GATGTGGATGAAGATGATATAGAGAAGGCTAATGCTCCTTCATGTGACCGTCTAGAAGATCTGGCCTCAC TGGTATACCTCAACGAGTCTAGCGTCCTGCATACACTGCGCCAGCGCTATGGGGCTAGCCTGCTACACAC CTATGCTGGCCCCAGCCTGCTTGTCCTTAGCACCCGAGGGGCTCCTGCTGTGTATTCAGAGAAGGTGATG CACATGTTCAAGGGGTGTCGGCGGGAGGACATGGCACCCCACATCTACGCTGTAGCCCAGACTGCATATA GGGCGATGTTGATGAGTCGTCAGGACCAGTCCATTGTCCTTCTGGGTAGTAGTGGCAGTGGCAAGACTAC CAGCTTTCAGCATCTGGTGCAGTATCTGGCTACCATCGCGGGTACCAGTGGGACCAAGGTGTTCTCAGTG GAGAAGTGGCAGGCTCTGTCCACCCTCCTGGAAGCCTTTGGGAACAGCCCCACCATCATGAATGGCAGTG CCACCCGCTTCTCCCAGATCCTCTCCCTGGACTTTGACCAAGCTGGCCAGGTGGCTTCGGCCTCCATCCA GACAATGCTCCTGGAGAAGCTGCGTGTGGCCCGGCGCCCAGCCAGTGAGGCTACTTTCAATGTCTTCTAC TACTTGCTGGCCTGTGGGGATGCCACCCTCAGGACAGAGCTCCACCTGAACCACTTGGCAGAGAACAATG TGTTTGGAATTGTGCCACTGTCCAAGCCTGAGGAGAAGCAGAAGGCTGCACAGCAGTTCAGTAAGCTCCA GGCTGCCATGAAGGTGCTGGCCATCTCCCCCGAGGAACAGAAGACCTGCTGGCTCATCCTGGCTTCCATC TACCACCTGGGGGCTGCTGGAGCTACCAAAGAGCCCCTTGAGGAGCAAGACGCTGCTGAAGCTGGACGAA AGCAGTTTGCCCGCCACGAATGGGCCCAGAAGGCAGCATACCTGCTGGGCTGCAGCTTGGAAGAGCTGTC CTCGGCTATCTTCAAACACCAGCTAAAAGGAGGCACCCTGCAACGGTCCACCTCCTTCCGTCAGGGCCCT GAGGAGAGCGGCCTGGGAGAGGGCACCAAACTGAGTGCTCTGGAGTGCTTAGAGGGTATGGCGTCTGGCC TCTACAGTGAGCTCTTTACTCTCCTCATCTCCCTGGTAAACAGGGCTCTCAAGTCCAGCCAGCACTCACT CTGTTCCATGATGATCGTGGACACACCAGGCTTCCAGAACCCTGAATGGGGCGGCTCGGCCCGTGGAGCC TCCTTCGAGGAGCTGTGCCACAACTATGCCCAGGACAGGCTGCAGAGGCTGTTCCACGAGCGCACCTTCC TCCAGGAGTTGGAGAGATACAAGGAGGATAACATAGAACTGGCGTTTGATGACTTGGAGCCAGTTGCAGA TGACTCCGTGGCTGCTGTGGACCAGGCTTCCCACCTGGTTCGCTCACTGGCCCATGCCGATGAGGCAAGA GGGCTACTGTGGCTCCTGGAAGAGGAGGCACTGGTGCCTGGTGCGACAGAGGATGCCCTCCTTGATCGTC TTTTCTCCTATTATGGCCCCCAGGAGGGTGACAAAAAAGGCCAGAGTCCTCTTCTGCGAAGTAGCAAACC TCGGCACTTTCTCCTGGGCCACAGCCACGGTACCAACTGGGTGGAGTACAACGTGGCTGGCTGGCTGAAT TACACCAAGCAGAACCCAGCCACCCAGAATGCACCCCGGCTTCTGCAGGACTCCCAGAAAAAGATCATCA GTAACCTGTTTCTGGGCCGGGCGGGCAGTGCCACGGTGCTCTCAGGCTCGATTGCAGGCCTGGAAGGTGG CTCCCAGCTGGCCTTGCGCCGGGCAACCAGCATGAGGAAAACCTTCACCACCGGCATGGCCGCTGTCAAG AAGAAGTCGCTGTGCATACAGATTAAGCTGCAAGTAGATGCCCTCATTGATACCATCAAGAGGTCCAAGA TGCATTTCGTGCACTGCTTCCTTCCTGTGGCCGAGGGCTGGCCTGGGGAGCCCCGGTCTGCCTCTTCCCG CAGAGTCAGCAGCAGCAGTGAGCTGGACCTGCCCCCGGGAGACCCCTGTGAGGCTGGGCTGCTACAACTG GATGTGTCCCTGCTCCGAGCCCAGCTCCGGGGATCCCGCCTGCTTGATGCGATGCGCATGTACCGCCAAG GTTACCCAGACCACATGGTGTTTTCTGAGTTCCGCCGGCGCTTTGATGTCCTGGCTCCACACCTGACCAA GAAACATGGTCGTAACTACATCGTGGTGGATGAGAAGCGGGCTGTGGAAGAGCTGCTGGAGTCCCTGGAC CTGGAAAAGAGCAGCTGCTGTCTGGGCCTAAGCCGGGTGTTCTTCCGGGCAGGCACGTTGGCACGGTTGG AAGAACAACGAGACGAGCAAACCAGCAGGCACCTGACCCTGTTCCAGGCAGCCTGCCGGGGCTACCTTGC CCGCCAGCACTTCAAGAAGAGAAAGATCCAGGACCTGGCCATTCGCTGTGTGCAGAAGAACATCAAGAAG AACAAGGGGGTGAAGGACTGGCCCTGGTGGAAGCTCTTCACCACCGTGCGGCCCCTCATCCAAGTTCAGC TGTCAGAGGAGCAGATCCGAAACAAAGACGAGGAGATCCAGCAGCTGCGCAGCAAGCTTGAGAAGGTGGA GAAGGAGAGGAACGAGCTCCGACTCAGCAGTGACCGGCTAGAGACCCGGATCTCAGAACTGACATCGGAG CTGACTGATGAACGCAACACGGGAGAGTCCGCCTCCCAGCTGCTGGACGCGGAGACAGCCGAGAGGCTCC GGACCGAGAAGGAGATGAAAGAGCTACAGACCCAGTATGACGCACTGAAGAAGCAGATGGAGGTGATGGA GATGGAAGTGATGGAAGCCCGGCTCATTCGGGCAGCCGAGATCAACGGGGAGGTGGATGATGATGATGCA GGGGGAGAGTGGAGGCTGAAGTACGAGCGAGCTGTTCGGGAGGTGGACTTCACCAAGAAGCGGCTCCAGC AGGAGCTGGAAGACAAGATGGAGGTGGAACAGCAGAGCAGGAGGCAGCTGGAGAGGCGGCTTGGGGACCT GCAAGCAGATAGTGATGAGAGTCAACGGGCACTGCAGCAGCTCAAGAAGAAGTGCCAAAGGCTCACAGCT GAGCTGCAGGACACCAAGCTGCACCTGGAGGGCCAGCAGGTCCGAAACCACGAGCTGGAGAAGAAGCAGA GGAGGTTCGACAGTGAGCTTTCCCAGGCCCACGAGGAGACGCAGCGGGAGAAGCTGCAGAGGGAGAAACT CCAGCGGGAGAAGGACATGCTCCTGGCTGAGGCTTTCAGCTTGAAGCAGCAAATGGAGGAAAAAGACTTG GACATTGCGGGGTTTACCCAGAAGGTGGTTTCCTTGGAGGCTGAGCTTCAGGACATTTCTTCTCAAGAGT CTAAGGATGAGGCTTCTCTGGCCAAGGTCAAGAAGCAGCTCCGGGACTTGGAGGCCAAGGTCAAGGACCA GGAAGAGGAGCTGGATGAGCAGGCTGGGAGTATACAGATGCTGGAGCAGGCCAAGCTGCGTCTGGAGATG GAGATGGAGAGAATGAGACAGACCCATTCCAAGGAGATGGAGAGTCGGGATGAGGAGGTGGAAGAGGCCC GGCAGTCATGTCAGAAGAAGTTAAAACAGATGGAAGTGCAGCTCGAGGAGGAGTATGAAGACAAGCAGAA GGCGCTGCGGGAGAAACGGGAGCTGGAGAGCAAGCTCTCCACGCTCAGTGACCAGGTGAACCAGCGGGAC TTTGAATCAGAGAAGCGGCTACGGAAAGACCTGAAGCGCACCAAAGCGCTGCTGGCAGATGCCCAGATCA TGCTGGACCACTTAAAGAACAATGCCCCGAGCAAGAGGGAGATTGCCCAGCTGAAGAACCAGCTGGAAGA GTCGGAATTCACCTGTGCAGCAGCTGTAAAGGCACGGAAAGCGATGGAGGTGGAGATGGAAGACCTGCAC CTGCAGATTGATGACATCGCCAAAGCCAAGACAGCGCTGGAGGAGCAACTGAGTCGACTTCAGCGTGAGA AAAACGAGATTCAAAACCGGCTGGAAGAGGATCAGGAGGACATGAATGAGCTGATGAAGAAGCACAAGGC AGCTGTGGCCCAGGCCTCCCGGGACATGGCACAGATGAACGATCTCCAGGCTCAGATTGAAGAGTCCAAC AAGGAGAAGCAAGAGCTACAGGAGAAGCTACAAGCTCTGCAGAGCCAGGTGGAGTTCCTGGAGCAGTCCA TGGTGGACAAGTCCCTCGTCAGCAGGCAGGAAGCGAAGATCAGGGAGCTGGAGACACGCCTGGAGTTCGA AAAGACCCAAGTGAAGCGCCTGGAGAACCTGGCCAGTCGGCTCAAAGAAACCATGGAGAAGCTGACTGAG GAACGGGACCAGCGCGCCGCAGCTGAGAACCGTGAGAAGGAGCAGAACAAGAGGCTCCAGCGACAGCTTC GTGACACCAAGGAGGAGATGAGCGAGCTTGCCAGGAAGGAAGCCGAGGCTAGCCGCAAGAAGCATGAACT GGAGATGGACCTGGAGAGCCTGGAAGCTGCTAACCAAAGCCTGCAAGCCGACCTAAAGCTGGCGTTCAAA CGCATTGGGGACTTGCAAGCTGCCATTGAGGATGAGATGGAAAGTGACGAGAACGAGGACCTCATCAACA GTGAGGGGGACTCAGATGTGGACTCAGAGCTGGAGGACCGGGTCGACGGGGTCAAGTCCTGGTTGTCAAA GAACAAGGGACCTTCTAAGGCACCTTCTGACGATGGCAGCTTGAAGAGTTCCAGCCCAACCAGCCACTGG AAGCCACTCGCCCCTGACCCATCGGATGATGAGCATGATCCTGTGGACAGCATCTCCAGACCCCGGTTCT CCCACAGCTATCTGAGTGACAGCGACACAGAGGCCAAGCTGACAGAGACCAGTGCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231868 representing NM_001291215 Red=Cloning site Green=Tags(s) MLLDPEAASPAYSQAKTEEQIAAEEAWYETEKVWLVHRDGFSLASQLKSEELSLPEGKARVKLDHDGAIL DVDEDDIEKANAPSCDRLEDLASLVYLNESSVLHTLRQRYGASLLHTYAGPSLLVLSTRGAPAVYSEKVM HMFKGCRREDMAPHIYAVAQTAYRAMLMSRQDQSIVLLGSSGSGKTTSFQHLVQYLATIAGTSGTKVFSV EKWQALSTLLEAFGNSPTIMNGSATRFSQILSLDFDQAGQVASASIQTMLLEKLRVARRPASEATFNVFY YLLACGDATLRTELHLNHLAENNVFGIVPLSKPEEKQKAAQQFSKLQAAMKVLAISPEEQKTCWLILASI YHLGAAGATKEPLEEQDAAEAGRKQFARHEWAQKAAYLLGCSLEELSSAIFKHQLKGGTLQRSTSFRQGP EESGLGEGTKLSALECLEGMASGLYSELFTLLISLVNRALKSSQHSLCSMMIVDTPGFQNPEWGGSARGA SFEELCHNYAQDRLQRLFHERTFLQELERYKEDNIELAFDDLEPVADDSVAAVDQASHLVRSLAHADEAR GLLWLLEEEALVPGATEDALLDRLFSYYGPQEGDKKGQSPLLRSSKPRHFLLGHSHGTNWVEYNVAGWLN YTKQNPATQNAPRLLQDSQKKIISNLFLGRAGSATVLSGSIAGLEGGSQLALRRATSMRKTFTTGMAAVK KKSLCIQIKLQVDALIDTIKRSKMHFVHCFLPVAEGWPGEPRSASSRRVSSSSELDLPPGDPCEAGLLQL DVSLLRAQLRGSRLLDAMRMYRQGYPDHMVFSEFRRRFDVLAPHLTKKHGRNYIVVDEKRAVEELLESLD LEKSSCCLGLSRVFFRAGTLARLEEQRDEQTSRHLTLFQAACRGYLARQHFKKRKIQDLAIRCVQKNIKK NKGVKDWPWWKLFTTVRPLIQVQLSEEQIRNKDEEIQQLRSKLEKVEKERNELRLSSDRLETRISELTSE LTDERNTGESASQLLDAETAERLRTEKEMKELQTQYDALKKQMEVMEMEVMEARLIRAAEINGEVDDDDA GGEWRLKYERAVREVDFTKKRLQQELEDKMEVEQQSRRQLERRLGDLQADSDESQRALQQLKKKCQRLTA ELQDTKLHLEGQQVRNHELEKKQRRFDSELSQAHEETQREKLQREKLQREKDMLLAEAFSLKQQMEEKDL DIAGFTQKVVSLEAELQDISSQESKDEASLAKVKKQLRDLEAKVKDQEEELDEQAGSIQMLEQAKLRLEM EMERMRQTHSKEMESRDEEVEEARQSCQKKLKQMEVQLEEEYEDKQKALREKRELESKLSTLSDQVNQRD FESEKRLRKDLKRTKALLADAQIMLDHLKNNAPSKREIAQLKNQLEESEFTCAAAVKARKAMEVEMEDLH LQIDDIAKAKTALEEQLSRLQREKNEIQNRLEEDQEDMNELMKKHKAAVAQASRDMAQMNDLQAQIEESN KEKQELQEKLQALQSQVEFLEQSMVDKSLVSRQEAKIRELETRLEFEKTQVKRLENLASRLKETMEKLTE ERDQRAAAENREKEQNKRLQRQLRDTKEEMSELARKEAEASRKKHELEMDLESLEAANQSLQADLKLAFK RIGDLQAAIEDEMESDENEDLINSEGDSDVDSELEDRVDGVKSWLSKNKGPSKAPSDDGSLKSSSPTSHW KPLAPDPSDDEHDPVDSISRPRFSHSYLSDSDTEAKLTETSA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001291215
ORF Size	5166 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001291215.1, NP_001278144.1
RefSeq Size	6417 bp
RefSeq ORF	5169 bp
Locus ID	360013
UniProt ID	Q9JMH9
MW	196.4 kDa
Gene Summary	May link Golgi membranes to the cytoskeleton and participate in the tensile force required for vesicle budding from the Golgi. Thereby, may play a role in Golgi membrane trafficking and could indirectly give its flattened shape to the Golgi apparatus (PubMed:19837035). Alternatively, in concert with LURAP1 and CDC42BPA/CDC42BPB, has been involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration (By similarity). May be involved in the maintenance of the stromal cell architectures required for cell to cell contact (PubMed:10733906). Regulates trafficking, expression, and activation of innate immune receptors on macrophages. Plays a role to suppress inflammatory responsiveness of macrophages via a mechanism that modulates CD14 trafficking (PubMed:25965346). Acts as a receptor of surfactant-associated protein A (SFTPA1/SP-A) and plays an important role in internalization and clearance of SFTPA1-opsonized S.aureus by alveolar macrophages (PubMed:21123169). Strongly enhances natural killer cell cytotoxicity (By similarity).[UniProtKB/Swiss-Prot Function]

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