Myo18a (NM_001291215) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	MAJN; MyoPDZ; MysPDZ; SP-R210
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229657 representing NM_001291215 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCTGGACCCCGAGGCCGCCTCTCCTGCCTACAGCCAGGCCAAGACGGAAGAGCAGATTGCCGCTG AAGAGGCCTGGTATGAGACGGAGAAGGTGTGGCTGGTCCATAGAGATGGATTCTCCTTAGCCAGCCAGCT CAAGTCTGAGGAGCTCAGCCTACCCGAGGGGAAGGCGCGTGTGAAGCTGGACCATGACGGAGCCATCCTG GATGTGGATGAAGATGATATAGAGAAGGCTAATGCTCCTTCATGTGACCGTCTAGAAGATCTGGCCTCAC TGGTATACCTCAACGAGTCTAGCGTCCTGCATACACTGCGCCAGCGCTATGGGGCTAGCCTGCTACACAC CTATGCTGGCCCCAGCCTGCTTGTCCTTAGCACCCGAGGGGCTCCTGCTGTGTATTCAGAGAAGGTGATG CACATGTTCAAGGGGTGTCGGCGGGAGGACATGGCACCCCACATCTACGCTGTAGCCCAGACTGCATATA GGGCGATGTTGATGAGTCGTCAGGACCAGTCCATTGTCCTTCTGGGTAGTAGTGGCAGTGGCAAGACTAC CAGCTTTCAGCATCTGGTGCAGTATCTGGCTACCATCGCGGGTACCAGTGGGACCAAGGTGTTCTCAGTG GAGAAGTGGCAGGCTCTGTCCACCCTCCTGGAAGCCTTTGGGAACAGCCCCACCATCATGAATGGCAGTG CCACCCGCTTCTCCCAGATCCTCTCCCTGGACTTTGACCAAGCTGGCCAGGTGGCTTCGGCCTCCATCCA GACAATGCTCCTGGAGAAGCTGCGTGTGGCCCGGCGCCCAGCCAGTGAGGCTACTTTCAATGTCTTCTAC TACTTGCTGGCCTGTGGGGATGCCACCCTCAGGACAGAGCTCCACCTGAACCACTTGGCAGAGAACAATG TGTTTGGAATTGTGCCACTGTCCAAGCCTGAGGAGAAGCAGAAGGCTGCACAGCAGTTCAGTAAGCTCCA GGCTGCCATGAAGGTGCTGGCCATCTCCCCCGAGGAACAGAAGACCTGCTGGCTCATCCTGGCTTCCATC TACCACCTGGGGGCTGCTGGAGCTACCAAAGAGCCCCTTGAGGAGCAAGACGCTGCTGAAGCTGGACGAA AGCAGTTTGCCCGCCACGAATGGGCCCAGAAGGCAGCATACCTGCTGGGCTGCAGCTTGGAAGAGCTGTC CTCGGCTATCTTCAAACACCAGCTAAAAGGAGGCACCCTGCAACGGTCCACCTCCTTCCGTCAGGGCCCT GAGGAGAGCGGCCTGGGAGAGGGCACCAAACTGAGTGCTCTGGAGTGCTTAGAGGGTATGGCGTCTGGCC TCTACAGTGAGCTCTTTACTCTCCTCATCTCCCTGGTAAACAGGGCTCTCAAGTCCAGCCAGCACTCACT CTGTTCCATGATGATCGTGGACACACCAGGCTTCCAGAACCCTGAATGGGGCGGCTCGGCCCGTGGAGCC TCCTTCGAGGAGCTGTGCCACAACTATGCCCAGGACAGGCTGCAGAGGCTGTTCCACGAGCGCACCTTCC TCCAGGAGTTGGAGAGATACAAGGAGGATAACATAGAACTGGCGTTTGATGACTTGGAGCCAGTTGCAGA TGACTCCGTGGCTGCTGTGGACCAGGCTTCCCACCTGGTTCGCTCACTGGCCCATGCCGATGAGGCAAGA GGGCTACTGTGGCTCCTGGAAGAGGAGGCACTGGTGCCTGGTGCGACAGAGGATGCCCTCCTTGATCGTC TTTTCTCCTATTATGGCCCCCAGGAGGGTGACAAAAAAGGCCAGAGTCCTCTTCTGCGAAGTAGCAAACC TCGGCACTTTCTCCTGGGCCACAGCCACGGTACCAACTGGGTGGAGTACAACGTGGCTGGCTGGCTGAAT TACACCAAGCAGAACCCAGCCACCCAGAATGCACCCCGGCTTCTGCAGGACTCCCAGAAAAAGATCATCA GTAACCTGTTTCTGGGCCGGGCGGGCAGTGCCACGGTGCTCTCAGGCTCGATTGCAGGCCTGGAAGGTGG CTCCCAGCTGGCCTTGCGCCGGGCAACCAGCATGAGGAAAACCTTCACCACCGGCATGGCCGCTGTCAAG AAGAAGTCGCTGTGCATACAGATTAAGCTGCAAGTAGATGCCCTCATTGATACCATCAAGAGGTCCAAGA TGCATTTCGTGCACTGCTTCCTTCCTGTGGCCGAGGGCTGGCCTGGGGAGCCCCGGTCTGCCTCTTCCCG CAGAGTCAGCAGCAGCAGTGAGCTGGACCTGCCCCCGGGAGACCCCTGTGAGGCTGGGCTGCTACAACTG GATGTGTCCCTGCTCCGAGCCCAGCTCCGGGGATCCCGCCTGCTTGATGCGATGCGCATGTACCGCCAAG GTTACCCAGACCACATGGTGTTTTCTGAGTTCCGCCGGCGCTTTGATGTCCTGGCTCCACACCTGACCAA GAAACATGGTCGTAACTACATCGTGGTGGATGAGAAGCGGGCTGTGGAAGAGCTGCTGGAGTCCCTGGAC CTGGAAAAGAGCAGCTGCTGTCTGGGCCTAAGCCGGGTGTTCTTCCGGGCAGGCACGTTGGCACGGTTGG AAGAACAACGAGACGAGCAAACCAGCAGGCACCTGACCCTGTTCCAGGCAGCCTGCCGGGGCTACCTTGC CCGCCAGCACTTCAAGAAGAGAAAGATCCAGGACCTGGCCATTCGCTGTGTGCAGAAGAACATCAAGAAG AACAAGGGGGTGAAGGACTGGCCCTGGTGGAAGCTCTTCACCACCGTGCGGCCCCTCATCCAAGTTCAGC TGTCAGAGGAGCAGATCCGAAACAAAGACGAGGAGATCCAGCAGCTGCGCAGCAAGCTTGAGAAGGTGGA GAAGGAGAGGAACGAGCTCCGACTCAGCAGTGACCGGCTAGAGACCCGGATCTCAGAACTGACATCGGAG CTGACTGATGAACGCAACACGGGAGAGTCCGCCTCCCAGCTGCTGGACGCGGAGACAGCCGAGAGGCTCC GGACCGAGAAGGAGATGAAAGAGCTACAGACCCAGTATGACGCACTGAAGAAGCAGATGGAGGTGATGGA GATGGAAGTGATGGAAGCCCGGCTCATTCGGGCAGCCGAGATCAACGGGGAGGTGGATGATGATGATGCA GGGGGAGAGTGGAGGCTGAAGTACGAGCGAGCTGTTCGGGAGGTGGACTTCACCAAGAAGCGGCTCCAGC AGGAGCTGGAAGACAAGATGGAGGTGGAACAGCAGAGCAGGAGGCAGCTGGAGAGGCGGCTTGGGGACCT GCAAGCAGATAGTGATGAGAGTCAACGGGCACTGCAGCAGCTCAAGAAGAAGTGCCAAAGGCTCACAGCT GAGCTGCAGGACACCAAGCTGCACCTGGAGGGCCAGCAGGTCCGAAACCACGAGCTGGAGAAGAAGCAGA GGAGGTTCGACAGTGAGCTTTCCCAGGCCCACGAGGAGACGCAGCGGGAGAAGCTGCAGAGGGAGAAACT CCAGCGGGAGAAGGACATGCTCCTGGCTGAGGCTTTCAGCTTGAAGCAGCAAATGGAGGAAAAAGACTTG GACATTGCGGGGTTTACCCAGAAGGTGGTTTCCTTGGAGGCTGAGCTTCAGGACATTTCTTCTCAAGAGT CTAAGGATGAGGCTTCTCTGGCCAAGGTCAAGAAGCAGCTCCGGGACTTGGAGGCCAAGGTCAAGGACCA GGAAGAGGAGCTGGATGAGCAGGCTGGGAGTATACAGATGCTGGAGCAGGCCAAGCTGCGTCTGGAGATG GAGATGGAGAGAATGAGACAGACCCATTCCAAGGAGATGGAGAGTCGGGATGAGGAGGTGGAAGAGGCCC GGCAGTCATGTCAGAAGAAGTTAAAACAGATGGAAGTGCAGCTCGAGGAGGAGTATGAAGACAAGCAGAA GGCGCTGCGGGAGAAACGGGAGCTGGAGAGCAAGCTCTCCACGCTCAGTGACCAGGTGAACCAGCGGGAC TTTGAATCAGAGAAGCGGCTACGGAAAGACCTGAAGCGCACCAAAGCGCTGCTGGCAGATGCCCAGATCA TGCTGGACCACTTAAAGAACAATGCCCCGAGCAAGAGGGAGATTGCCCAGCTGAAGAACCAGCTGGAAGA GTCGGAATTCACCTGTGCAGCAGCTGTAAAGGCACGGAAAGCGATGGAGGTGGAGATGGAAGACCTGCAC CTGCAGATTGATGACATCGCCAAAGCCAAGACAGCGCTGGAGGAGCAACTGAGTCGACTTCAGCGTGAGA AAAACGAGATTCAAAACCGGCTGGAAGAGGATCAGGAGGACATGAATGAGCTGATGAAGAAGCACAAGGC AGCTGTGGCCCAGGCCTCCCGGGACATGGCACAGATGAACGATCTCCAGGCTCAGATTGAAGAGTCCAAC AAGGAGAAGCAAGAGCTACAGGAGAAGCTACAAGCTCTGCAGAGCCAGGTGGAGTTCCTGGAGCAGTCCA TGGTGGACAAGTCCCTCGTCAGCAGGCAGGAAGCGAAGATCAGGGAGCTGGAGACACGCCTGGAGTTCGA AAAGACCCAAGTGAAGCGCCTGGAGAACCTGGCCAGTCGGCTCAAAGAAACCATGGAGAAGCTGACTGAG GAACGGGACCAGCGCGCCGCAGCTGAGAACCGTGAGAAGGAGCAGAACAAGAGGCTCCAGCGACAGCTTC GTGACACCAAGGAGGAGATGAGCGAGCTTGCCAGGAAGGAAGCCGAGGCTAGCCGCAAGAAGCATGAACT GGAGATGGACCTGGAGAGCCTGGAAGCTGCTAACCAAAGCCTGCAAGCCGACCTAAAGCTGGCGTTCAAA CGCATTGGGGACTTGCAAGCTGCCATTGAGGATGAGATGGAAAGTGACGAGAACGAGGACCTCATCAACA GTGAGGGGGACTCAGATGTGGACTCAGAGCTGGAGGACCGGGTCGACGGGGTCAAGTCCTGGTTGTCAAA GAACAAGGGACCTTCTAAGGCACCTTCTGACGATGGCAGCTTGAAGAGTTCCAGCCCAACCAGCCACTGG AAGCCACTCGCCCCTGACCCATCGGATGATGAGCATGATCCTGTGGACAGCATCTCCAGACCCCGGTTCT CCCACAGCTATCTGAGTGACAGCGACACAGAGGCCAAGCTGACAGAGACCAGTGCATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001291215
Insert Size	5169 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001291215.1, NP_001278144.1
RefSeq Size	6417 bp
RefSeq ORF	5169 bp
Locus ID	360013
UniProt ID	Q9JMH9
Gene Summary	May link Golgi membranes to the cytoskeleton and participate in the tensile force required for vesicle budding from the Golgi. Thereby, may play a role in Golgi membrane trafficking and could indirectly give its flattened shape to the Golgi apparatus (PubMed:19837035). Alternatively, in concert with LURAP1 and CDC42BPA/CDC42BPB, has been involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration (By similarity). May be involved in the maintenance of the stromal cell architectures required for cell to cell contact (PubMed:10733906). Regulates trafficking, expression, and activation of innate immune receptors on macrophages. Plays a role to suppress inflammatory responsiveness of macrophages via a mechanism that modulates CD14 trafficking (PubMed:25965346). Acts as a receptor of surfactant-associated protein A (SFTPA1/SP-A) and plays an important role in internalization and clearance of SFTPA1-opsonized S.aureus by alveolar macrophages (PubMed:21123169). Strongly enhances natural killer cell cytotoxicity (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (5) contains an alternate 5'-terminal exon and has multiple coding region differences, compared to variant 1. The encoded isoform (5) is shorter and has a distinct N-terminus, compared to isoform 1.

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