Camsap1 (NM_001276360) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	9530003A05Rik; C77823; PRO2405
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231846 representing NM_001276360 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGGACGCGGGCGGTCGCTGTGCCGCCGAAGGCTGGAGGAGGATGGAGGCGCCCCCGGAGGGCGCCG ACCTGGTGCCGCTGGACCGCTACGACGCGGCGCGGGCCAAGATCGCCGCCAACCTGCAGTGGATCTGTGC GAAGGCCTATGGCCTAGACAACATCCCTGAGGACCTCCGAGACCCGTTTTACATCGACCAGTATGAGCAG GAGCACATTAAGCCACCCGTTATCAAGCTTCTCCTGTCCAGTGAGCTGTATTGCCGTGTCTGCAGCCTCA TCCTAAAAGGGGACCAGGTGGCTACCTTGCAAGGACACCAGTCTGTCATCCAGGCCCTGTCCCGGAAGGG CATCTATGTGATGGAGAGTGATGATACCCCTGTGACAGATGCTGACCTCAGCCAGGCACCTATTAAGATG AGTGGCCACATGGCGATGGTGGATGCCCTGATGATGGCCTACACTGTGGAGATGATCAGCATTGAGAAGG TGGTGGCCAGTGTCAAGCGCTTTTCTACATTCAGCGCCTCAAAAGAACTTCCTTATGACCTCGAGGATGC CATGGTATTCTGGATCAATAAGGTAAATCTTAAAATGAGAGAGATAACAGAGAAAGAAGTTAAATTAAAA CAGCAGCCACTGGAAAGTCCCGCTCATCAAAAGTCTCCCTCCAAGTGGTATTGGAAGTTAGTACCCGTCC GTTATCGGCGAGAGCACCTCTCTGCTAGGCAGTCACCCTACTTCCCGTTGTTGGAGGACTTGATGAGAGA CGGCAGTGATGGCGCTGCTCTCTTAGCAGTGGTTCACTATTATTGCCCAGAGCAGATGAAACTGGATGAT ATCTGTCTGAAGGAGGTGCCATCAATGGCTGACAGCCTCTATAACATCCGCCTTCTGAGGGAGTTTTCCA ACGAGCATCTGAACAAGTGTTTCTATCTCACGCTGGAGGATATGCTGTATGCCCCCCTAGTGCTGAAGCC GAACGTTATGGTTTTTATCGCTGAGCTCTTCTGGTGGTTTGAGAACGTCAAACCAGATTTTGTTCAGCCC CGAGATATTCAAGAGCTGAAGGATGCTAAAACAGTGTTACAGCAGAAGAGCAGCCGGCCTCCTGTCCCTA TCTCCAATGCAACCAAACGCAGCTTCCTGGGTAGCCCTGCTGCCATGAGCCCCGCTGACCAGCCACCATC CACCCAGCCTCTTGCTGAAGGCAGTCATCGGTACCACCTCCACTCGGAAGAACCTGAGTGTCTTGGGAAA GGAGCTTCCACATTTAGTCCGTCCCATCCTTTGTTGCCACTGAGACAGAAACAGCAAAAAGTGTCACAGA CAGAGGAAATCCCTGATCAGCGGCACAGGTCGAATTCTTTAACTCGGGTTGATGGTCAGCCACGAGGTGC AATAGGAGCATGGCCAGATAAAAAAAACAGGCCTGTGTCACAGCCAACATCATTTGCTCTCCATCATGCT GCAAGTTGTGATGTGGATCCCAGTTCTGGTGACAGTGTCAGCTTAGCTCGCTCTATCAGCAAAGATAGCT TGGCATCTAACATTATCCACCTGACCCCACAGAACCAGCCCCATCCCTCCGCTGGAAAGAGCAATGGGAA AAGCCTCCTGAGCAATGTCAACATTGAGGATGAGGACGAGGAGCTTGTAGCCATCATCAGGACAGATGTG TCTCCTCCTTCCCCACAGATGCCCAGGACCTCACCTCAGGCCCCAGGCCTGGTAGCAAGTATCAGGTCTC CCCAGAGACAGGCAGACACTTTGGAGAGTAAGCCTGACAGTTTTTACTTAGAACCCCTGATGCCAGCAGT ACTCCGGCCAGCCAAAGAGAAGCAGATTACCACTAAGGAAGATGAGCGTGGAGAAGGAAGGCCAAGGACC ATCATGGCCAAGAGGCCTAGTGAGGGCTCCCAGCCTATGGTACGAAAGAAAGTAAGTGGTGGCCATGGCA GTCGTGACCTGAACAGAACTTTCACCCCAATTCCTTGTTCAGAATTTGCTGCGAGCATTGATCTTGCGGA GGTAGGACCACAGTCAGCAGAAGCTACAGGGGAAGGACAGCCTCTGGCCCTGGGTAGATTTGATACATTA CCTCAGGGGCAGGCTGCTGATGGTTTCTTTCTCCATGTAGGCAGGGCTGAGGAAGATGAGGGGAGGTGGT ACGTTGGTTCACAAAGTCCTAGCTCCCACGACTCAGAACCCTGGACCATTTTGAGGCAAGACTCGGACTC TGATGTAGTAGACGTAGAGGATACAGAGCAGGATTTTATTGGTGAGGACCACCCTGTGGTTATCCCCAGA TATGCTGGTGAGGAAGAGTCAGCCAAACTACAGGAGGATATGAAAGTGAAGGAACATGAAGACAAGGATG ATGCCAGCGGCCGCTCAAGCCCGTGTCTGAGCACCACTTCTCAGCTCAGCAGCATGTCCATGGCCAGTGG AAGTGTAAAGATGACCAGCTTTGCTGAAAGGAAGCTCCAGCGGCTCAACAGCTGTGAGACCAAGTCCAGC ACCAGCAGCTCCCAGAAGACCACCCCTGATGCATCTGAAAGCTGCCCAGCCCCTCTGACAACGTGGCGCC AGAAGAGGGAGCAGAGTCCAGGCAGGCACAGCAAGGACCCTGCCAGCCTGCTGGCTTCTGAGCTGGTGCA GCTGCACATGCAGCTGGAGGAAAAGCGCAGAGCTATTGAAGCCCAGAAGAAAAAGATGGAGGCGCTGTCC GCACGGCAGCGTCTGAAGCTGGGCAAGGCAGCCTTTCTGCATGTGGTAAAGAAAGGGAAGGCTGATGGTG CCCCACAGCCACTGAGACCCGAACACTTCACAAAGGAATTCACACAGCACAATGGGGAGGACTTGGATGA TGGCACTTGTAAAACTGAAGGGTTCCTTGTCAAAGAAGAGCAGAGAGATCTCAGTGACGCTCAGGATGTG GCATTTGTCCAGCTACATAAGCCCAGGGACCCAGCTGCTCTGCATGATGGAGAGAAGCACAGAATGATTT CTACGGCCCTCCTCGAGGATAGTGTTGGTGAGGTAGATGTGAACGAGTGTGACCTGTCCATTGAGAAGCT CAATGAGACCATCAGTACATTGCAGCAGGCTATACTGAAGATCTCCCAGCAGCAAGAGCAGCTTCTCATG AAGTCCCCCACAGTGCCAACACCGGGCACTAAAAATAACTGCCAGGACCAAAAAATAAAGGCCCCAGTCC ACTTTGTTGAGCCACTCTCTCCAACTGGCGTGCCTGGCCACCGCAAACCACCACGGCTTGGCCAGGGCCG GAATTCCCGCTCAGGAAGGCCGGCTGAACTGAAGGTTCCAAAAGACAGGCAGCAGGGTTGTTCTCGGAGC AAAACCCCAACACCCAGTGTGGAGACACTCCCACAGTCAAGGTCTTTACCTCCAAGTACCCATCCACGGT CACCCTCCGACCCAGGCGGGGAACTCCCTGAGAAGTGTCTCTTCGACAGTTATAGGCTCCATGATGAGAG TAACCATCGGACATTTGTTCTGTCCTCTTGCAAAGATGCAAACATTGTATCAGAACAGGTGAACTTTAAA GAGGGTCTAGATACAAGTGTGAAAGAGGCAGGGCTGAGCTCCTCTACCATCACAGGCAAAGAACATACTC CAGTGGAAGAGCCGCTGAGGAGCAAGGCCAGCCTCATTGAGGTAGACCTCTCTGACCTGAAGGCCCCTGA TGAGGATGGAGAGGTGGTTGGCCATGAGAGCTCGGTGGAGCTTGGTGGAGATAGTGACCAGAAGCCTGGG GTTGGTTTCTTCTTCAAGGATGAACAGAAAGCAGAAGATGAGCTTGCTAAGAAGCGGGCAGCCTTCCTTT TGAAACAGCAGCGCAAGGCTGAAGAGGCTCGTGCGCGCAAGCAGCAGCTGGAGGCAGAAGTGGAGCTCAA GCGAGATGAAGCCCGGCGTAAGGCTGAAGAGGATCGACTACGGAAGGAGGAGGAGAAGGCAAGGCGAGAG CTCATTAAACAGGAATACTTACGGAGGAAGCAGCAGCAGGCCTTGGAGGAGCAAGGACTTGGCAAACCTA AATCAAAGCCTAAAAAGCCTCGGCCAAAGTCAGTTCACCGGGAAGAGTCTTACAGCGACTCTGGCACCAA GTGCTCTTCTACCCATAACTTGAGCCAAACTCACTCTGGCTCCAGCCTATCCTTGGCATCTGCAGCAACA ACAGAACCTGAGAGTGTTTATTCGGGGGGCACACCTTCTCACCGAGTTGAATCACTGGAAGCTTTACCTA TCCTGAGCCGCAACCCCAGTCGGAGCACAGACCGAGACTGGGAGACTGCATCAGCAGCTTCCTCTTTGGC CTCTGTGGCTGAGTACACAGGTCCTAAACTCTTTAAGGAGCCCAGTAGCAAATCAAACAAACCAATTATT CACAATGCCATCTCCCACTGCTGTCTGGCTGGAAAAGTGAATGAGCCTCACAAGAATTCAATATTGGAGG AGCTGGAGAAGTGTGATGCCAACCACTATATTATTCTGTTCCGAGATGCGGGCTGCCAGTTCAGGGCACT TTACTGCTACCAACCCGACACTGAAGAAATCTACAAACTCACTGGCACGGGGCCAAAAAGCATCACCAAG AAGATGATTGACAAACTATATAAATACAGCTCAGACCGAAAGCAGTTTAACCTGATCCCAGCCAAGACCA TGTCAGTCAGCGTGGATGCACTCACGATCCATAACCACTTGTGGCAGCCCAAGCGGCCCACGGTGCCAAA GAAGACCCAGACTCGTAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231846 representing NM_001276360 Red=Cloning site Green=Tags(s) MVDAGGRCAAEGWRRMEAPPEGADLVPLDRYDAARAKIAANLQWICAKAYGLDNIPEDLRDPFYIDQYEQ EHIKPPVIKLLLSSELYCRVCSLILKGDQVATLQGHQSVIQALSRKGIYVMESDDTPVTDADLSQAPIKM SGHMAMVDALMMAYTVEMISIEKVVASVKRFSTFSASKELPYDLEDAMVFWINKVNLKMREITEKEVKLK QQPLESPAHQKSPSKWYWKLVPVRYRREHLSARQSPYFPLLEDLMRDGSDGAALLAVVHYYCPEQMKLDD ICLKEVPSMADSLYNIRLLREFSNEHLNKCFYLTLEDMLYAPLVLKPNVMVFIAELFWWFENVKPDFVQP RDIQELKDAKTVLQQKSSRPPVPISNATKRSFLGSPAAMSPADQPPSTQPLAEGSHRYHLHSEEPECLGK GASTFSPSHPLLPLRQKQQKVSQTEEIPDQRHRSNSLTRVDGQPRGAIGAWPDKKNRPVSQPTSFALHHA ASCDVDPSSGDSVSLARSISKDSLASNIIHLTPQNQPHPSAGKSNGKSLLSNVNIEDEDEELVAIIRTDV SPPSPQMPRTSPQAPGLVASIRSPQRQADTLESKPDSFYLEPLMPAVLRPAKEKQITTKEDERGEGRPRT IMAKRPSEGSQPMVRKKVSGGHGSRDLNRTFTPIPCSEFAASIDLAEVGPQSAEATGEGQPLALGRFDTL PQGQAADGFFLHVGRAEEDEGRWYVGSQSPSSHDSEPWTILRQDSDSDVVDVEDTEQDFIGEDHPVVIPR YAGEEESAKLQEDMKVKEHEDKDDASGRSSPCLSTTSQLSSMSMASGSVKMTSFAERKLQRLNSCETKSS TSSSQKTTPDASESCPAPLTTWRQKREQSPGRHSKDPASLLASELVQLHMQLEEKRRAIEAQKKKMEALS ARQRLKLGKAAFLHVVKKGKADGAPQPLRPEHFTKEFTQHNGEDLDDGTCKTEGFLVKEEQRDLSDAQDV AFVQLHKPRDPAALHDGEKHRMISTALLEDSVGEVDVNECDLSIEKLNETISTLQQAILKISQQQEQLLM KSPTVPTPGTKNNCQDQKIKAPVHFVEPLSPTGVPGHRKPPRLGQGRNSRSGRPAELKVPKDRQQGCSRS KTPTPSVETLPQSRSLPPSTHPRSPSDPGGELPEKCLFDSYRLHDESNHRTFVLSSCKDANIVSEQVNFK EGLDTSVKEAGLSSSTITGKEHTPVEEPLRSKASLIEVDLSDLKAPDEDGEVVGHESSVELGGDSDQKPG VGFFFKDEQKAEDELAKKRAAFLLKQQRKAEEARARKQQLEAEVELKRDEARRKAEEDRLRKEEEKARRE LIKQEYLRRKQQQALEEQGLGKPKSKPKKPRPKSVHREESYSDSGTKCSSTHNLSQTHSGSSLSLASAAT TEPESVYSGGTPSHRVESLEALPILSRNPSRSTDRDWETASAASSLASVAEYTGPKLFKEPSSKSNKPII HNAISHCCLAGKVNEPHKNSILEELEKCDANHYIILFRDAGCQFRALYCYQPDTEEIYKLTGTGPKSITK KMIDKLYKYSSDRKQFNLIPAKTMSVSVDALTIHNHLWQPKRPTVPKKTQTRK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001276360
ORF Size	4779 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001276360.1, NP_001263289.1
RefSeq Size	8029 bp
RefSeq ORF	4782 bp
Locus ID	227634
UniProt ID	A2AHC3
MW	177.8 kDa
Gene Summary	Key microtubule-organizing protein that specifically binds the minus-end of non-centrosomal microtubules and regulates their dynamics and organization. Specifically recognizes growing microtubule minus-ends and stabilizes microtubules. Acts on free microtubule minus-ends that are not capped by microtubule-nucleating proteins or other factors and protects microtubule minus-ends from depolymerization. In contrast to CAMSAP2 and CAMSAP3, tracks along the growing tips of minus-end microtubules without significantly affecting the polymerization rate: binds at the very tip of the microtubules minus-end and acts as a minus-end tracking protein (-TIP) that dissociates from microtubules after allowing tubulin incorporation. Through interaction with spectrin may regulate neurite outgrowth.[UniProtKB/Swiss-Prot Function]

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