Eif4g3 (NM_001256198) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	1500002J22Rik; 4930523M17Rik; eIF4GII; G1-419-52; repro8
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231809 representing NM_001256198 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACTCACAACCTCAGGCCCGGTCTCCGGGAGGATTCAGACCCATCCAGTTTTTCCAGAGGCCTCAAA TCCAGCCTCCCCGAGCTGCCATCCCAAACAGCAGTCCTTCCATCCGTCCCGGTGTGCAGACCCCCACCGC CGTGTATCAGGCCAACCAGCACATCATGATGGTTAACCATCTGCCCATGCCGTACCCCGTGACCCAGGGG CATCAGTACTGCATACCACAGTACCGCCACAGCGGCCCTCCGTACGTGGGGCCCCCACAGCAGTATCCAG TTCAGCCGCCTGGGCCGGGGCCTTTTTATCCTGGACCAGGACCTGGGGACTTCGCTAATGCTTATGGAAC GCCTTTTTACCCAAGTCAGCCAGTGTATCAGTCAGCACCTATCATAGTGCCTACGCAGCAGCAGCCCCCT CCAGCCAAGAGAGAGAAAAAAACTATAAGAATTCGAGATCCAAATCAGGGAGGTAAAGACATAACAGAGG AGATTATGTCTGGAGGTGGCAGCAGAAATCCCACTCCACCTATAGGAAGACCCGCATCCACTCCCACTCC TCCTCAGCAGCTGCCCAGCCAGGTCCCTGAGCACAGCCCTGTGGTATATGGGACTGTGGAGAGCGCTCAT CTTGCTGCCAGCACCCCTGTCACTGCAGCTAGCGACCAGAAGCAAGAAGAAAAGCCAAAACCAGATCCAG TGTTCCAGTCTCCTTCGACGGTCCTTAGGCTCGTACTCAGTGGGGAGAAGAAAGAGCAAGCAGGCCAGAT GCCTGAGACTGCTGCAGGAGAGCCCACCCCAGAGCCTCCTCGGACTTCATCACCCACCAGCCTTCCTCCA CTTGCCCGGAGTTCACTTCCTTCCCCCATGTCCGCTGCCCTTAGCAGCCAGCCACTGTTCACTGCTGAGG ACAAGTGTGAACTTCCTTCCTCCAAAGAAGAAGATGCACCTCCTGTCCCCAGCCCCACGTCTTGCACAGC AGCCTCAGGCCCTTCACTAACAGATAACAGTGATATATGCAAGAAACCCTGTAGCGTAGCGCCTCATGAT AGTCAGCTAATTTCTAGTACTATCCTAATTAATGAAATGAATGGAGTTGGAGAAAAATTATCAGCCAAGG AGAACACTGTGGGCATGCTGAGACAGGAAGTGCTGCCATTGACTCTCGAGTTAGAGATTCTGGAACATCC ACAGGAAGAATTGAAAGTGGAATGTACGCCGACTCCCATTGCCCCGTCCATGCTGCCTGCCTTCTCCCCA GCTCCTCCGACTCCCCCCACTTCTCCTCCTTGTCCTCCGGTTGTCCTCTCTGCCGCCATTGCTCGTTCTC CCGCTGTTGCCACTGAGGTCCAGAGAGTCGCCGACGAGGGAGAAAGCCTGAGAACTTGCCTTAGCAAGGA TGCAAAGGAGATGCAGGACAAAGCAGAGTCAGAATCAGATGGGCAAGCAGAAGAGACTGCGGATCCCCAG AGCCTACATTCAGGAAGGAGCCCCGCCCCAGTGCAAACAGCTACCACTGCGCCAAAGTCCTGGAAGAAAA CAAAAGAGCAGACCCGAACCCCAGATGAAGTGTTAGAAGCAGAGGCAGAGCCTAAAGCCGAAGAAGAGCT TGCAGTTGACAGTGTCCTTGAGCCTGAGCAAGAGAAGATGAGCCAAGGGTTTCCGTCTGAGAGAGACCCC TCTGCCCTGAAGAGAGGGAAAGCTGAGGAAGGAAACGGAGAAGAGGCTGAGCCTGTGCGAAATGGCGCTG AGAGTGCTTCTGAGGGGGAAGGGGGAGATGGCAACTCAGGCTCTGCTGACAGCTCTGCTGATGGGCTCAC CTTCCCGTTTAAAGCAGAGTCCTGGAAGCCTGCAGATACAGAAGGCAAGAAGCAGTATGACCGGGAGTTC CTACTGGACATCCAGTTTATGCCCGCCTGTATCCAAAAACCAGAAGGCCTGCCTCCCATCAGCGACGTGG TCCTTGACAAGATCAACCAGCCCAGATTGTCAATGCGGACCCTGGATCCTCGCATTTTGCCTCGAGGACC TGACTTCACCCCAGCCTTTGCAGACTTTCCAAGGCAAACGCCTGGTGGAAGAGGTGTGCCTCTGTTGAAT GTTGGACCTCGGAGGTCTCAACCTGGCCAGAGAAGGGAGCCCAGAAAGATAATCACTGTCTCCGTAAAAG AAGATGTGCACTTGAGAAAGGCGGAGAATGCCTGGAAGCCGAGCCAGAAACGGGACAGCCACGCCGATGA CCCCGAAAGCATTAAAACTCAGGAACTTTTCAGAAAAGTTCGAAGTATCTTGAATAAACTGACACCACAG ATGTTCAACCAGCTGATGAAGCAAGTGTCGGCTCTTACGGTGGACACGGAGGAGCGGCTAAAAGGGGTCA TCGACCTTGTCTTTGAGAAGGCTATCGATGAGCCCAGCTTCTCTGTGGCGTACGCAAACATGTGTCGGTG CCTAGTAACGCTGAAGGTGCCCATGGCAGACAAGCCTGGCAACACAGTGAATTTCCGGAAGCTGCTGCTT AACCGCTGCCAGAAGGAGTTCGAGAAAGACAAAGCAGATGATGACGTCTTTGAGAAGAAGCAGAAAGAAC TGGAAGCTGCCAGCGCTCCAGAGGAGAGGACAAGGCTTCACGACGAACTGGAAGAAGCCAAGGACAAAGC CCGGCGGAGATCCATCGGCAACATCAAGTTCATTGGAGAACTCTTTAAACTGAAAATGCTGACGGAGGCC ATCATGCATGACTGTGTGGTGAAGCTCCTGAAGAACCACGATGAAGAGTCCCTGGAGTGCCTGTGTCGCC TGCTCACCACCATCGGCAAAGACCTGGACTTTGAGAAAGCAAAGCCACGCATGGACCAGTACTTTAACCA GATGGAGAAAATCGTCAAAGAAAGAAAAACTTCCTCCAGAATTCGGTTCATGCTTCAGGATGTAATCGAC CTCAGACTGTGTAATTGGGTGTCCCGAAGAGCAGATCAGGGGCCAAAAACTATCGAGCAGATTCACAAAG AGGCCAAAATAGAAGAACAAGAGGAGCAGAGGAAAGTCCAGCAGCTGATGACCAAGGAGAAGAGGAGACC AGGCGTCCAAAGAGTGGATGAAGGTGGATGGAATACTGTACAAGGGGCCAAGAACAGTCGTGTACTGGAC CCCTCCAAATTTTTGAAAATTACTAAACCCACCATTGATGAGAAGATTCAGCTGGTTCCTAAGGCACAGC TGGGCAGCTGGGGCAAAGGCAGCAGCGGGGGAGCAAAGGCAAGTGAGTCCGATGCCTTACGGTCAAGTGC TTCCAGTTTAAATAGATTCTCTCCTCTGCAACCTCCAGCACCTTCAGGGTCCCCGTCGGCCACACCCTTA GAGTTTGATTCCCGAAGGGCGTTAACCAGTCGTGGGAGCATGGGCAGGGAGAAGAGCGACAAGCCAATTC CAGCTGGAACAGCCCGTCCCAACACGTTCCTGAGGGGCAGCAGCAAAGACCTGCTGGACAACCAGTCCCA GGAAGAGCAGCGCAGAGAGATGTTGGAGACCGTGAAACAGCTGACAGGAGGCCTGGATGCTGAGCGGGCC AGCACTGAGGCCGACCGAAGCAAAACGAGGGAGCTAGCAAAATCGGAAATGTGTGCAGTGCCAGCCCCTG ACAAGCCTGCACTGTCAGAAGAGGAAGTGGAGAGGAAGTCCAAGTCTATCATTGACGAATTCTTACACAT CAATGACTTTAAGGAGGCCACGCAGTGCATAGAGGAGCTGAGCGCCCAGGGCCCACTGCATGTGTTCGTG AAGGTGGGTGTGGAGTTCACCCTGGAACGGAGCCAGATCACCAGGGACCACATGGGCCACTTACTGTATC AGCTGGTGCAGTCAGAAAAACTCAGCAAGCAGGACTTTTTCAAAGGTTTTTCTGAAACCTTGGAGTTGGC AGATGACATGGCCATTGATATTCCCCATATTTGGTTGTACCTGGCTGAACTGGTCACCCCCATGTTAAAA GGAGGGGGGATTTCCATGAGAGAACTTATTGTGGAATTCAGCAAGCCATTACTTCCTGTTGGCAGAGCTG GGGTCCTGCTTTCTGAAATCCTACACCTTCTATGCAGACAAATGAGCCATAAGAAAGTAGGCGCCCTGTG GAGGGAGGCTGACCTCAGCTGGAAGGACTTTTTACCGGAAGGGGAAGATGTCCATCATTTTCTCTTGGAG CAGAAGTTGGACTTCACGGAATCCGAAGGGCCCTGCTCCTCTGAGGCACTGTCAAAGAAGGAGCTGTCTG CCGAGGAGCTGTCTCAGCGCCTGGAAAAGCTCATCATGGAGGAGAAAGCGGATGACGAGCGGATCTTTGA CTGGGTGGAGACTTGCTCCGGATGTTTTTTGATTGCCTGTATGACGAGGAGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231809 representing NM_001256198 Red=Cloning site Green=Tags(s) MNSQPQARSPGGFRPIQFFQRPQIQPPRAAIPNSSPSIRPGVQTPTAVYQANQHIMMVNHLPMPYPVTQG HQYCIPQYRHSGPPYVGPPQQYPVQPPGPGPFYPGPGPGDFANAYGTPFYPSQPVYQSAPIIVPTQQQPP PAKREKKTIRIRDPNQGGKDITEEIMSGGGSRNPTPPIGRPASTPTPPQQLPSQVPEHSPVVYGTVESAH LAASTPVTAASDQKQEEKPKPDPVFQSPSTVLRLVLSGEKKEQAGQMPETAAGEPTPEPPRTSSPTSLPP LARSSLPSPMSAALSSQPLFTAEDKCELPSSKEEDAPPVPSPTSCTAASGPSLTDNSDICKKPCSVAPHD SQLISSTILINEMNGVGEKLSAKENTVGMLRQEVLPLTLELEILEHPQEELKVECTPTPIAPSMLPAFSP APPTPPTSPPCPPVVLSAAIARSPAVATEVQRVADEGESLRTCLSKDAKEMQDKAESESDGQAEETADPQ SLHSGRSPAPVQTATTAPKSWKKTKEQTRTPDEVLEAEAEPKAEEELAVDSVLEPEQEKMSQGFPSERDP SALKRGKAEEGNGEEAEPVRNGAESASEGEGGDGNSGSADSSADGLTFPFKAESWKPADTEGKKQYDREF LLDIQFMPACIQKPEGLPPISDVVLDKINQPRLSMRTLDPRILPRGPDFTPAFADFPRQTPGGRGVPLLN VGPRRSQPGQRREPRKIITVSVKEDVHLRKAENAWKPSQKRDSHADDPESIKTQELFRKVRSILNKLTPQ MFNQLMKQVSALTVDTEERLKGVIDLVFEKAIDEPSFSVAYANMCRCLVTLKVPMADKPGNTVNFRKLLL NRCQKEFEKDKADDDVFEKKQKELEAASAPEERTRLHDELEEAKDKARRRSIGNIKFIGELFKLKMLTEA IMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIVKERKTSSRIRFMLQDVID LRLCNWVSRRADQGPKTIEQIHKEAKIEEQEEQRKVQQLMTKEKRRPGVQRVDEGGWNTVQGAKNSRVLD PSKFLKITKPTIDEKIQLVPKAQLGSWGKGSSGGAKASESDALRSSASSLNRFSPLQPPAPSGSPSATPL EFDSRRALTSRGSMGREKSDKPIPAGTARPNTFLRGSSKDLLDNQSQEEQRREMLETVKQLTGGLDAERA STEADRSKTRELAKSEMCAVPAPDKPALSEEEVERKSKSIIDEFLHINDFKEATQCIEELSAQGPLHVFV KVGVEFTLERSQITRDHMGHLLYQLVQSEKLSKQDFFKGFSETLELADDMAIDIPHIWLYLAELVTPMLK GGGISMRELIVEFSKPLLPVGRAGVLLSEILHLLCRQMSHKKVGALWREADLSWKDFLPEGEDVHHFLLE QKLDFTESEGPCSSEALSKKELSAEELSQRLEKLIMEEKADDERIFDWVETCSGCFLIACMTRR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001256198
ORF Size	4392 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001256198.1, NP_001243127.1
RefSeq Size	6069 bp
RefSeq ORF	4395 bp
Locus ID	230861
MW	162.3 kDa
Gene Summary	Probable component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome. Thought to be a functional homolog of EIF4G1 (By similarity).[UniProtKB/Swiss-Prot Function]

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