Eif4g3 (NM_001256198) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1500002J22Rik; 4930523M17Rik; eIF4GII; G1-419-52; repro8
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229598 representing NM_001256198 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACTCACAACCTCAGGCCCGGTCTCCGGGAGGATTCAGACCCATCCAGTTTTTCCAGAGGCCTCAAA TCCAGCCTCCCCGAGCTGCCATCCCAAACAGCAGTCCTTCCATCCGTCCCGGTGTGCAGACCCCCACCGC CGTGTATCAGGCCAACCAGCACATCATGATGGTTAACCATCTGCCCATGCCGTACCCCGTGACCCAGGGG CATCAGTACTGCATACCACAGTACCGCCACAGCGGCCCTCCGTACGTGGGGCCCCCACAGCAGTATCCAG TTCAGCCGCCTGGGCCGGGGCCTTTTTATCCTGGACCAGGACCTGGGGACTTCGCTAATGCTTATGGAAC GCCTTTTTACCCAAGTCAGCCAGTGTATCAGTCAGCACCTATCATAGTGCCTACGCAGCAGCAGCCCCCT CCAGCCAAGAGAGAGAAAAAAACTATAAGAATTCGAGATCCAAATCAGGGAGGTAAAGACATAACAGAGG AGATTATGTCTGGAGGTGGCAGCAGAAATCCCACTCCACCTATAGGAAGACCCGCATCCACTCCCACTCC TCCTCAGCAGCTGCCCAGCCAGGTCCCTGAGCACAGCCCTGTGGTATATGGGACTGTGGAGAGCGCTCAT CTTGCTGCCAGCACCCCTGTCACTGCAGCTAGCGACCAGAAGCAAGAAGAAAAGCCAAAACCAGATCCAG TGTTCCAGTCTCCTTCGACGGTCCTTAGGCTCGTACTCAGTGGGGAGAAGAAAGAGCAAGCAGGCCAGAT GCCTGAGACTGCTGCAGGAGAGCCCACCCCAGAGCCTCCTCGGACTTCATCACCCACCAGCCTTCCTCCA CTTGCCCGGAGTTCACTTCCTTCCCCCATGTCCGCTGCCCTTAGCAGCCAGCCACTGTTCACTGCTGAGG ACAAGTGTGAACTTCCTTCCTCCAAAGAAGAAGATGCACCTCCTGTCCCCAGCCCCACGTCTTGCACAGC AGCCTCAGGCCCTTCACTAACAGATAACAGTGATATATGCAAGAAACCCTGTAGCGTAGCGCCTCATGAT AGTCAGCTAATTTCTAGTACTATCCTAATTAATGAAATGAATGGAGTTGGAGAAAAATTATCAGCCAAGG AGAACACTGTGGGCATGCTGAGACAGGAAGTGCTGCCATTGACTCTCGAGTTAGAGATTCTGGAACATCC ACAGGAAGAATTGAAAGTGGAATGTACGCCGACTCCCATTGCCCCGTCCATGCTGCCTGCCTTCTCCCCA GCTCCTCCGACTCCCCCCACTTCTCCTCCTTGTCCTCCGGTTGTCCTCTCTGCCGCCATTGCTCGTTCTC CCGCTGTTGCCACTGAGGTCCAGAGAGTCGCCGACGAGGGAGAAAGCCTGAGAACTTGCCTTAGCAAGGA TGCAAAGGAGATGCAGGACAAAGCAGAGTCAGAATCAGATGGGCAAGCAGAAGAGACTGCGGATCCCCAG AGCCTACATTCAGGAAGGAGCCCCGCCCCAGTGCAAACAGCTACCACTGCGCCAAAGTCCTGGAAGAAAA CAAAAGAGCAGACCCGAACCCCAGATGAAGTGTTAGAAGCAGAGGCAGAGCCTAAAGCCGAAGAAGAGCT TGCAGTTGACAGTGTCCTTGAGCCTGAGCAAGAGAAGATGAGCCAAGGGTTTCCGTCTGAGAGAGACCCC TCTGCCCTGAAGAGAGGGAAAGCTGAGGAAGGAAACGGAGAAGAGGCTGAGCCTGTGCGAAATGGCGCTG AGAGTGCTTCTGAGGGGGAAGGGGGAGATGGCAACTCAGGCTCTGCTGACAGCTCTGCTGATGGGCTCAC CTTCCCGTTTAAAGCAGAGTCCTGGAAGCCTGCAGATACAGAAGGCAAGAAGCAGTATGACCGGGAGTTC CTACTGGACATCCAGTTTATGCCCGCCTGTATCCAAAAACCAGAAGGCCTGCCTCCCATCAGCGACGTGG TCCTTGACAAGATCAACCAGCCCAGATTGTCAATGCGGACCCTGGATCCTCGCATTTTGCCTCGAGGACC TGACTTCACCCCAGCCTTTGCAGACTTTCCAAGGCAAACGCCTGGTGGAAGAGGTGTGCCTCTGTTGAAT GTTGGACCTCGGAGGTCTCAACCTGGCCAGAGAAGGGAGCCCAGAAAGATAATCACTGTCTCCGTAAAAG AAGATGTGCACTTGAGAAAGGCGGAGAATGCCTGGAAGCCGAGCCAGAAACGGGACAGCCACGCCGATGA CCCCGAAAGCATTAAAACTCAGGAACTTTTCAGAAAAGTTCGAAGTATCTTGAATAAACTGACACCACAG ATGTTCAACCAGCTGATGAAGCAAGTGTCGGCTCTTACGGTGGACACGGAGGAGCGGCTAAAAGGGGTCA TCGACCTTGTCTTTGAGAAGGCTATCGATGAGCCCAGCTTCTCTGTGGCGTACGCAAACATGTGTCGGTG CCTAGTAACGCTGAAGGTGCCCATGGCAGACAAGCCTGGCAACACAGTGAATTTCCGGAAGCTGCTGCTT AACCGCTGCCAGAAGGAGTTCGAGAAAGACAAAGCAGATGATGACGTCTTTGAGAAGAAGCAGAAAGAAC TGGAAGCTGCCAGCGCTCCAGAGGAGAGGACAAGGCTTCACGACGAACTGGAAGAAGCCAAGGACAAAGC CCGGCGGAGATCCATCGGCAACATCAAGTTCATTGGAGAACTCTTTAAACTGAAAATGCTGACGGAGGCC ATCATGCATGACTGTGTGGTGAAGCTCCTGAAGAACCACGATGAAGAGTCCCTGGAGTGCCTGTGTCGCC TGCTCACCACCATCGGCAAAGACCTGGACTTTGAGAAAGCAAAGCCACGCATGGACCAGTACTTTAACCA GATGGAGAAAATCGTCAAAGAAAGAAAAACTTCCTCCAGAATTCGGTTCATGCTTCAGGATGTAATCGAC CTCAGACTGTGTAATTGGGTGTCCCGAAGAGCAGATCAGGGGCCAAAAACTATCGAGCAGATTCACAAAG AGGCCAAAATAGAAGAACAAGAGGAGCAGAGGAAAGTCCAGCAGCTGATGACCAAGGAGAAGAGGAGACC AGGCGTCCAAAGAGTGGATGAAGGTGGATGGAATACTGTACAAGGGGCCAAGAACAGTCGTGTACTGGAC CCCTCCAAATTTTTGAAAATTACTAAACCCACCATTGATGAGAAGATTCAGCTGGTTCCTAAGGCACAGC TGGGCAGCTGGGGCAAAGGCAGCAGCGGGGGAGCAAAGGCAAGTGAGTCCGATGCCTTACGGTCAAGTGC TTCCAGTTTAAATAGATTCTCTCCTCTGCAACCTCCAGCACCTTCAGGGTCCCCGTCGGCCACACCCTTA GAGTTTGATTCCCGAAGGGCGTTAACCAGTCGTGGGAGCATGGGCAGGGAGAAGAGCGACAAGCCAATTC CAGCTGGAACAGCCCGTCCCAACACGTTCCTGAGGGGCAGCAGCAAAGACCTGCTGGACAACCAGTCCCA GGAAGAGCAGCGCAGAGAGATGTTGGAGACCGTGAAACAGCTGACAGGAGGCCTGGATGCTGAGCGGGCC AGCACTGAGGCCGACCGAAGCAAAACGAGGGAGCTAGCAAAATCGGAAATGTGTGCAGTGCCAGCCCCTG ACAAGCCTGCACTGTCAGAAGAGGAAGTGGAGAGGAAGTCCAAGTCTATCATTGACGAATTCTTACACAT CAATGACTTTAAGGAGGCCACGCAGTGCATAGAGGAGCTGAGCGCCCAGGGCCCACTGCATGTGTTCGTG AAGGTGGGTGTGGAGTTCACCCTGGAACGGAGCCAGATCACCAGGGACCACATGGGCCACTTACTGTATC AGCTGGTGCAGTCAGAAAAACTCAGCAAGCAGGACTTTTTCAAAGGTTTTTCTGAAACCTTGGAGTTGGC AGATGACATGGCCATTGATATTCCCCATATTTGGTTGTACCTGGCTGAACTGGTCACCCCCATGTTAAAA GGAGGGGGGATTTCCATGAGAGAACTTATTGTGGAATTCAGCAAGCCATTACTTCCTGTTGGCAGAGCTG GGGTCCTGCTTTCTGAAATCCTACACCTTCTATGCAGACAAATGAGCCATAAGAAAGTAGGCGCCCTGTG GAGGGAGGCTGACCTCAGCTGGAAGGACTTTTTACCGGAAGGGGAAGATGTCCATCATTTTCTCTTGGAG CAGAAGTTGGACTTCACGGAATCCGAAGGGCCCTGCTCCTCTGAGGCACTGTCAAAGAAGGAGCTGTCTG CCGAGGAGCTGTCTCAGCGCCTGGAAAAGCTCATCATGGAGGAGAAAGCGGATGACGAGCGGATCTTTGA CTGGGTGGAGACTTGCTCCGGATGTTTTTTGATTGCCTGTATGACGAGGAGGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001256198
Insert Size	4395 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001256198.1, NP_001243127.1
RefSeq Size	6069 bp
RefSeq ORF	4395 bp
Locus ID	230861
Gene Summary	Probable component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome. Thought to be a functional homolog of EIF4G1 (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) contains an alternate exon in the 5' UTR and lacks an alternate, in-frame, exon in the 3' coding region which causes a shift in the reading frame, compared to variant 1. The resulting protein (isoform 3) has a shorter and distinct C-terminus when it is compared to isoform 1.

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