Eif4g3 (NM_001256198) Mouse Untagged Clone

CAT#: MC229598

Eif4g3 (untagged) - Mouse eukaryotic translation initiation factor 4 gamma, 3 (Eif4g3), transcript variant 3



  "NM_001256198" in other vectors (1)

CNY 14,060.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 1500002J22Rik; 4930523M17Rik; eIF4GII; G1-419-52; repro8
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC229598 representing NM_001256198
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACTCACAACCTCAGGCCCGGTCTCCGGGAGGATTCAGACCCATCCAGTTTTTCCAGAGGCCTCAAA
TCCAGCCTCCCCGAGCTGCCATCCCAAACAGCAGTCCTTCCATCCGTCCCGGTGTGCAGACCCCCACCGC
CGTGTATCAGGCCAACCAGCACATCATGATGGTTAACCATCTGCCCATGCCGTACCCCGTGACCCAGGGG
CATCAGTACTGCATACCACAGTACCGCCACAGCGGCCCTCCGTACGTGGGGCCCCCACAGCAGTATCCAG
TTCAGCCGCCTGGGCCGGGGCCTTTTTATCCTGGACCAGGACCTGGGGACTTCGCTAATGCTTATGGAAC
GCCTTTTTACCCAAGTCAGCCAGTGTATCAGTCAGCACCTATCATAGTGCCTACGCAGCAGCAGCCCCCT
CCAGCCAAGAGAGAGAAAAAAACTATAAGAATTCGAGATCCAAATCAGGGAGGTAAAGACATAACAGAGG
AGATTATGTCTGGAGGTGGCAGCAGAAATCCCACTCCACCTATAGGAAGACCCGCATCCACTCCCACTCC
TCCTCAGCAGCTGCCCAGCCAGGTCCCTGAGCACAGCCCTGTGGTATATGGGACTGTGGAGAGCGCTCAT
CTTGCTGCCAGCACCCCTGTCACTGCAGCTAGCGACCAGAAGCAAGAAGAAAAGCCAAAACCAGATCCAG
TGTTCCAGTCTCCTTCGACGGTCCTTAGGCTCGTACTCAGTGGGGAGAAGAAAGAGCAAGCAGGCCAGAT
GCCTGAGACTGCTGCAGGAGAGCCCACCCCAGAGCCTCCTCGGACTTCATCACCCACCAGCCTTCCTCCA
CTTGCCCGGAGTTCACTTCCTTCCCCCATGTCCGCTGCCCTTAGCAGCCAGCCACTGTTCACTGCTGAGG
ACAAGTGTGAACTTCCTTCCTCCAAAGAAGAAGATGCACCTCCTGTCCCCAGCCCCACGTCTTGCACAGC
AGCCTCAGGCCCTTCACTAACAGATAACAGTGATATATGCAAGAAACCCTGTAGCGTAGCGCCTCATGAT
AGTCAGCTAATTTCTAGTACTATCCTAATTAATGAAATGAATGGAGTTGGAGAAAAATTATCAGCCAAGG
AGAACACTGTGGGCATGCTGAGACAGGAAGTGCTGCCATTGACTCTCGAGTTAGAGATTCTGGAACATCC
ACAGGAAGAATTGAAAGTGGAATGTACGCCGACTCCCATTGCCCCGTCCATGCTGCCTGCCTTCTCCCCA
GCTCCTCCGACTCCCCCCACTTCTCCTCCTTGTCCTCCGGTTGTCCTCTCTGCCGCCATTGCTCGTTCTC
CCGCTGTTGCCACTGAGGTCCAGAGAGTCGCCGACGAGGGAGAAAGCCTGAGAACTTGCCTTAGCAAGGA
TGCAAAGGAGATGCAGGACAAAGCAGAGTCAGAATCAGATGGGCAAGCAGAAGAGACTGCGGATCCCCAG
AGCCTACATTCAGGAAGGAGCCCCGCCCCAGTGCAAACAGCTACCACTGCGCCAAAGTCCTGGAAGAAAA
CAAAAGAGCAGACCCGAACCCCAGATGAAGTGTTAGAAGCAGAGGCAGAGCCTAAAGCCGAAGAAGAGCT
TGCAGTTGACAGTGTCCTTGAGCCTGAGCAAGAGAAGATGAGCCAAGGGTTTCCGTCTGAGAGAGACCCC
TCTGCCCTGAAGAGAGGGAAAGCTGAGGAAGGAAACGGAGAAGAGGCTGAGCCTGTGCGAAATGGCGCTG
AGAGTGCTTCTGAGGGGGAAGGGGGAGATGGCAACTCAGGCTCTGCTGACAGCTCTGCTGATGGGCTCAC
CTTCCCGTTTAAAGCAGAGTCCTGGAAGCCTGCAGATACAGAAGGCAAGAAGCAGTATGACCGGGAGTTC
CTACTGGACATCCAGTTTATGCCCGCCTGTATCCAAAAACCAGAAGGCCTGCCTCCCATCAGCGACGTGG
TCCTTGACAAGATCAACCAGCCCAGATTGTCAATGCGGACCCTGGATCCTCGCATTTTGCCTCGAGGACC
TGACTTCACCCCAGCCTTTGCAGACTTTCCAAGGCAAACGCCTGGTGGAAGAGGTGTGCCTCTGTTGAAT
GTTGGACCTCGGAGGTCTCAACCTGGCCAGAGAAGGGAGCCCAGAAAGATAATCACTGTCTCCGTAAAAG
AAGATGTGCACTTGAGAAAGGCGGAGAATGCCTGGAAGCCGAGCCAGAAACGGGACAGCCACGCCGATGA
CCCCGAAAGCATTAAAACTCAGGAACTTTTCAGAAAAGTTCGAAGTATCTTGAATAAACTGACACCACAG
ATGTTCAACCAGCTGATGAAGCAAGTGTCGGCTCTTACGGTGGACACGGAGGAGCGGCTAAAAGGGGTCA
TCGACCTTGTCTTTGAGAAGGCTATCGATGAGCCCAGCTTCTCTGTGGCGTACGCAAACATGTGTCGGTG
CCTAGTAACGCTGAAGGTGCCCATGGCAGACAAGCCTGGCAACACAGTGAATTTCCGGAAGCTGCTGCTT
AACCGCTGCCAGAAGGAGTTCGAGAAAGACAAAGCAGATGATGACGTCTTTGAGAAGAAGCAGAAAGAAC
TGGAAGCTGCCAGCGCTCCAGAGGAGAGGACAAGGCTTCACGACGAACTGGAAGAAGCCAAGGACAAAGC
CCGGCGGAGATCCATCGGCAACATCAAGTTCATTGGAGAACTCTTTAAACTGAAAATGCTGACGGAGGCC
ATCATGCATGACTGTGTGGTGAAGCTCCTGAAGAACCACGATGAAGAGTCCCTGGAGTGCCTGTGTCGCC
TGCTCACCACCATCGGCAAAGACCTGGACTTTGAGAAAGCAAAGCCACGCATGGACCAGTACTTTAACCA
GATGGAGAAAATCGTCAAAGAAAGAAAAACTTCCTCCAGAATTCGGTTCATGCTTCAGGATGTAATCGAC
CTCAGACTGTGTAATTGGGTGTCCCGAAGAGCAGATCAGGGGCCAAAAACTATCGAGCAGATTCACAAAG
AGGCCAAAATAGAAGAACAAGAGGAGCAGAGGAAAGTCCAGCAGCTGATGACCAAGGAGAAGAGGAGACC
AGGCGTCCAAAGAGTGGATGAAGGTGGATGGAATACTGTACAAGGGGCCAAGAACAGTCGTGTACTGGAC
CCCTCCAAATTTTTGAAAATTACTAAACCCACCATTGATGAGAAGATTCAGCTGGTTCCTAAGGCACAGC
TGGGCAGCTGGGGCAAAGGCAGCAGCGGGGGAGCAAAGGCAAGTGAGTCCGATGCCTTACGGTCAAGTGC
TTCCAGTTTAAATAGATTCTCTCCTCTGCAACCTCCAGCACCTTCAGGGTCCCCGTCGGCCACACCCTTA
GAGTTTGATTCCCGAAGGGCGTTAACCAGTCGTGGGAGCATGGGCAGGGAGAAGAGCGACAAGCCAATTC
CAGCTGGAACAGCCCGTCCCAACACGTTCCTGAGGGGCAGCAGCAAAGACCTGCTGGACAACCAGTCCCA
GGAAGAGCAGCGCAGAGAGATGTTGGAGACCGTGAAACAGCTGACAGGAGGCCTGGATGCTGAGCGGGCC
AGCACTGAGGCCGACCGAAGCAAAACGAGGGAGCTAGCAAAATCGGAAATGTGTGCAGTGCCAGCCCCTG
ACAAGCCTGCACTGTCAGAAGAGGAAGTGGAGAGGAAGTCCAAGTCTATCATTGACGAATTCTTACACAT
CAATGACTTTAAGGAGGCCACGCAGTGCATAGAGGAGCTGAGCGCCCAGGGCCCACTGCATGTGTTCGTG
AAGGTGGGTGTGGAGTTCACCCTGGAACGGAGCCAGATCACCAGGGACCACATGGGCCACTTACTGTATC
AGCTGGTGCAGTCAGAAAAACTCAGCAAGCAGGACTTTTTCAAAGGTTTTTCTGAAACCTTGGAGTTGGC
AGATGACATGGCCATTGATATTCCCCATATTTGGTTGTACCTGGCTGAACTGGTCACCCCCATGTTAAAA
GGAGGGGGGATTTCCATGAGAGAACTTATTGTGGAATTCAGCAAGCCATTACTTCCTGTTGGCAGAGCTG
GGGTCCTGCTTTCTGAAATCCTACACCTTCTATGCAGACAAATGAGCCATAAGAAAGTAGGCGCCCTGTG
GAGGGAGGCTGACCTCAGCTGGAAGGACTTTTTACCGGAAGGGGAAGATGTCCATCATTTTCTCTTGGAG
CAGAAGTTGGACTTCACGGAATCCGAAGGGCCCTGCTCCTCTGAGGCACTGTCAAAGAAGGAGCTGTCTG
CCGAGGAGCTGTCTCAGCGCCTGGAAAAGCTCATCATGGAGGAGAAAGCGGATGACGAGCGGATCTTTGA
CTGGGTGGAGACTTGCTCCGGATGTTTTTTGATTGCCTGTATGACGAGGAGGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001256198
Insert Size 4395 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001256198.1, NP_001243127.1
RefSeq Size 6069 bp
RefSeq ORF 4395 bp
Locus ID 230861
Gene Summary Probable component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome. Thought to be a functional homolog of EIF4G1 (By similarity).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (3) contains an alternate exon in the 5' UTR and lacks an alternate, in-frame, exon in the 3' coding region which causes a shift in the reading frame, compared to variant 1. The resulting protein (isoform 3) has a shorter and distinct C-terminus when it is compared to isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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