Lmtk3 (NM_001290990) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	aatyk3; BC059845
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231797 representing NM_001290990 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTGCACCCGGCGCACTCATCCTCCTGGCGGCCGTCTCCGCCTCCGGCTGCCTGGCGTCCCCAGCGC ACCCGGATGGATTCGCTCTGAGCCGGGCCCCTTTGGCTCCGCCCTACGCTGTGGTCCTCATTTCCTGTTC GGGTCTGCTGGCCTTCATCTTTCTCCTCCTTACCTGTCTGTGTTGCAAACGGGGTGATGTTCGTTTCAAG GAGTTTGAGAACCCGGAAGGGGAGGACTGCTCTGGGGAGTACACCCCCCCTGCGGAGGAGACCTCCTCCT CACAGTCGCTGCCTGATGTCTATATTCTGCCGCTGGCAGAGGTCTCACTGCCAATGCCTGCCCCGCAGCC TCCACACTCAGACATCAGCACCCCCCTGGGCCTGAGCCGCCAGCACCTCAGCTACCTGCAGGAGATTGGC AGCGGCTGGTTTGGGAAGGTGATCCTCGGGGAGGTTTTCTCAGACTACTCGCCAGCCCAGGTGGTGGTGA AGGAACTCCGGGCTAGTGCCGGGCCCCTGGAACAGCGCAAGTTCATCTCCGAGGCTCAGCCCTACAGGAG CCTGCAGCATCCCAACGTCCTCCAGTGCCTGGGCGTCTGTGTGGAGACCTTGCCCTTCCTGTTGATCATG GAGTTCTGCCAGCTGGGGGACCTGAAGCGATACCTTCGGGCCCAGCGGCCACCTGAAGGCATGTCCCCTG AACTTCCCCCTCGAGACCTTCGGACACTGCAGAGGATGGGTCTAGAGATTGCCCGAGGACTGGCACACCT GCACTCTCACAACTATGTGCACAGCGATCTGGCGCTGCGCAACTGCCTGCTAACTTCAGACCTGACTGTG CGTATTGGAGACTATGGGCTGGCGCACAGCAACTACAAGGAAGACTACTACCTGACACCCGAGCGCCTAT GGGTGCCGCTGCGCTGGGCAGCGCCCGAGCTGCTGGGCGAGCTGCACGGCAGCTTCGTGCTGGTAGATCA AAGCCGGGAGAGTAATGTCTGGTCCCTGGGGGTGACACTCTGGGAGCTATTCGAGTTCGGGGCACAGCCC TATCGTCACCTATCGGATGAGGAGGTCCTGGCCTTTGTTGTCCGCCAGCAGCACGTGAAGCTGGCCCGGC CCAGGCTCAAGCTGCCCTACGCTGACTATTGGTATGACATTTTGCAGTCTTGCTGGCGGCCGCCAGCCCA GCGCCCCTCAGCTTCTGATCTCCAGCTGCAGCTCACTTACCTGCTGTCTGAGCGGCCCCCCAGGCCCCCT CCTCCACCACCCCCTCCCCGAGATGGGCCCTTCCCCTGGCCCTGGCCCCCCTCGCATAGTGCGCCGCGCC CAGGGACCCTGTCCTCCCAGTTCCCCCTTCTGGATGGCTTCCCCGGGGCTGACCCTGATGATGTACTCAC AGTCACCGAGAGCAGCCGTGGCCTCAACCTTGAGTGCCTGTGGGAGAAGGCCCGCCGTGGGGCAGGCCGG GGTGGGGGTGCACCTCCCTGGCAACCCGCTTCTGCGCCTCCTGCGCCCCATACCAATCCATCCAATCCCT TCTATGAGGCGCTGTCCACCCCTAGCGTGCTTCCGGTCATCAGCGCACGCAGTCCCTCGGTGAGCAGCGA GTACTATATCCGCCTGGAGGAGCACGGTTCTCCACCAGAGCCCCTCTTCCCCAACGACTGGGACCCGCTG GACCCAGGAGTACCCGGTCCCCAGGCCCCCCAGACTCCCTCCGAGGTCCCTCAGCTGGTGTCCGAGACCT GGGCTTCCCCCCTCTTCCCCGCGCCCCGACCCTTCCCGGCCCAGTCCTCGGGATCAGGTGGTTTCCTGCT GAGCGGCTGGGACCCCGAGGGCCGGGGCGCAGGAGAGACCCTGGCAGGAGATCCTGCCGAGGTGCTTGGG GAACAGGGTACCGCACCCTGGGCCGAAGAGGAGGAGGAAGAGAGCTCCCCAGGCGAGGACAGCAGCAGCC TTGGAGGGGGACCCAGCCGCAGGGGACCCCTCCCCTGTCCCTTGTGCAGCCGCGAGGGTCCCTGCTCCTG TCTGCCATTGGAGCGGGGGGACGCTGTGGCCGGCTGGGGGGACCACCCTGCCCTTGGTTGTCCCCACCCC CCAGAGGACGACTCTTCCTTGCGTGCAGAGCGGGGCTCCTTGGCCGACCTGCCTCTGGTCCCCCCTACCT CAGCCCCTCTCGAGTTTCTGGACCCCCTTATGGGGGCCGCAGCGCCCCAGTACCCCGGGCGGGGGCCACC TCCCGCTCCCCCCCCTCCACCGCCCCCTCCCCGGGCCTCCGCGGAACCGGCCGCGTCCCCCGACCCCCCG TCGGCCCTGGCCAGTCCAGGCTCCGGCCTATCGTCTCCGGGCCCCAAGCCGGGGGACAGCGGCTACGAGA CCGAGACCCCTTTTTCCCCCGAGGGAGCCTTCCCAGGTGGGGGGGCGGCCGAGGAGGAAGGGGTCCCTCG GCCACGGGCTCCCCCCGAGCCACCCGACCCAGGAGCGCCCCGGCCACCCCCAGACCCGGGTCCCCTCCCA CTCCCAGGGTCCCAGGAGAAGCCAACCTTTGTAGTTCAGGTGAGCACCGAGCAGCTGCTGATGTCCCTAC GGGAGGATGTGACAAAGAACCTCCTAGGGGACAAGGGGTCGACACCCGGGGAGACAGGACCCAGGAAGGC GGGGAGAAGCCCCGCGAACAGAGAGAAGGGCCCAGGCCCGAACAGGGACCTGACATCCCTGGTCAGCAGG AAGAAAGTCCCCAGCAGGAGCCTTCCAGTGAACGGGGTGACAGTGTTGGAGAACGGGAAGCCAGGAGTCC CGGACATGAAGGAGAAGGTGGCGGAGAATGGCCTGGAATCTCCGGAGAAAGAAGAGAGAGCCCTGGTGAA TGGGGAGCCGATGTCCCCAGAGGCCGGGGAGAAGGTGCTGGCGAATGGGGTTCTGATGTCCCCAAAGAGC GAGGAGAAGGTGGCAGAGAATGGGGTCCTGAGGCTGCCCAGGAACACGGAGAGGCCACCAGAGATTGGAC CTCGGAGAGTCCCAGGGCCCTGGGAGAAGACGCCCGAGACTGGGGGTCTAGCTCCCGAGACCCTGCTGGA TCGAGCCCCTGCGCCCTGCGAGGCAGCCTTGCCCCAGAACGGCTTGGAGATGGCCCCTGGCCAGCTTGGC CCAGCCCCCAAGAGCGGGAACCCAGACCCCGGGACCGAGTGGAGAGTCCACGAGAGTGGGGGGGCACCGA GAGCCCCCGGGGCTGGGAAGCTGGACCTCGGGAGTGGGGGCCGAGCCCTGGGGGGCGTGGGGACGGCCCC CGCCGGCGGCCCCGCAAGCGCCGTGGACGCAAAGGCCGGATGGGTAGACAACTCGAGACCACTGCCACCT CCGCCACAGCCACTGGGGGCCCAACAGAGGAGGCCGGAGCCAGTGCCCCTGAAAGCCAGGCCGGAGGTGG CCCAAGAGGAAGAGCCAGGGGTCCCAGACAACAGGCTCGGCGGAGACATGGCCCCCAGCGTAGACGAGGA CCCCCTCAAGCCGGAGAGGAAGGGCCCCGAGATGCCACGCTTGTTCCTGGACTTGGGACCACCTCAGGGG AACAGCGAGCAGATCAAAGCCAAACTCTCCCGGCTCTCGCTGGCGCTCCCACCGCTCACGCTCACGCCGT TCCCGGGCCCGGGCCCGCGGCGGCCACCTTGGGAGGGCGCGGACGCAGGGGCAGCTGGCGGGGAGGCCGG CGGGGCGGGGGCGCCGGGGCCAGCGGAGGAGGACGGGGAGGACGAGGACGAGGACGAGGAGGACGAGGAG GCAGCGGGCTCTCGGGATCCCGGGAGGACGCGGGAAGCCCCAGTGCCCGTCGTGGTGAGCAGCGCCGACG GGGACACGGTCCGCCCGCTGCGGGGGCTGCTCAAGTCTCCACGCGCGGCCGACGAGCCAGAGGACAGCGA GCTGGAGAGGAAGCGCAAGATGGTCTCCTTCCACGGGGACGTGACCGTCTACCTCTTCGACCAGGAGACT CCAACCAACGAGTTGAGCGTCCAGGGCACCCCCGAGGGGGACACGGAGCCATCAACTCCCCCAGCGCCCC CGACGCCTCCCCACCCCACCACCCCAGGAGATGGGTTTCCCAACAGCGACAGCGGCTTTGGCGGCAGTTT CGAGTGGGCGGAGGATTTCCCCCTCCTCCCCCCGCCAGGCCCCCCCCTGTGCTTCTCCCGCTTCTCCGTC TCACCTGCACTGGAGACCCCGGGGCCTCCCGCCCGGGCTCCCGACGCCCGGCCCGCAGGCCCCGTGGAGA AC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231797 representing NM_001290990 Red=Cloning site Green=Tags(s) MPAPGALILLAAVSASGCLASPAHPDGFALSRAPLAPPYAVVLISCSGLLAFIFLLLTCLCCKRGDVRFK EFENPEGEDCSGEYTPPAEETSSSQSLPDVYILPLAEVSLPMPAPQPPHSDISTPLGLSRQHLSYLQEIG SGWFGKVILGEVFSDYSPAQVVVKELRASAGPLEQRKFISEAQPYRSLQHPNVLQCLGVCVETLPFLLIM EFCQLGDLKRYLRAQRPPEGMSPELPPRDLRTLQRMGLEIARGLAHLHSHNYVHSDLALRNCLLTSDLTV RIGDYGLAHSNYKEDYYLTPERLWVPLRWAAPELLGELHGSFVLVDQSRESNVWSLGVTLWELFEFGAQP YRHLSDEEVLAFVVRQQHVKLARPRLKLPYADYWYDILQSCWRPPAQRPSASDLQLQLTYLLSERPPRPP PPPPPPRDGPFPWPWPPSHSAPRPGTLSSQFPLLDGFPGADPDDVLTVTESSRGLNLECLWEKARRGAGR GGGAPPWQPASAPPAPHTNPSNPFYEALSTPSVLPVISARSPSVSSEYYIRLEEHGSPPEPLFPNDWDPL DPGVPGPQAPQTPSEVPQLVSETWASPLFPAPRPFPAQSSGSGGFLLSGWDPEGRGAGETLAGDPAEVLG EQGTAPWAEEEEEESSPGEDSSSLGGGPSRRGPLPCPLCSREGPCSCLPLERGDAVAGWGDHPALGCPHP PEDDSSLRAERGSLADLPLVPPTSAPLEFLDPLMGAAAPQYPGRGPPPAPPPPPPPPRASAEPAASPDPP SALASPGSGLSSPGPKPGDSGYETETPFSPEGAFPGGGAAEEEGVPRPRAPPEPPDPGAPRPPPDPGPLP LPGSQEKPTFVVQVSTEQLLMSLREDVTKNLLGDKGSTPGETGPRKAGRSPANREKGPGPNRDLTSLVSR KKVPSRSLPVNGVTVLENGKPGVPDMKEKVAENGLESPEKEERALVNGEPMSPEAGEKVLANGVLMSPKS EEKVAENGVLRLPRNTERPPEIGPRRVPGPWEKTPETGGLAPETLLDRAPAPCEAALPQNGLEMAPGQLG PAPKSGNPDPGTEWRVHESGGAPRAPGAGKLDLGSGGRALGGVGTAPAGGPASAVDAKAGWVDNSRPLPP PPQPLGAQQRRPEPVPLKARPEVAQEEEPGVPDNRLGGDMAPSVDEDPLKPERKGPEMPRLFLDLGPPQG NSEQIKAKLSRLSLALPPLTLTPFPGPGPRRPPWEGADAGAAGGEAGGAGAPGPAEEDGEDEDEDEEDEE AAGSRDPGRTREAPVPVVVSSADGDTVRPLRGLLKSPRAADEPEDSELERKRKMVSFHGDVTVYLFDQET PTNELSVQGTPEGDTEPSTPPAPPTPPHPTTPGDGFPNSDSGFGGSFEWAEDFPLLPPPGPPLCFSRFSV SPALETPGPPARAPDARPAGPVEN TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001290990
ORF Size	4272 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001290990.2
RefSeq Size	5022 bp
RefSeq ORF	4275 bp
Locus ID	381983
UniProt ID	Q5XJV6
MW	151.3 kDa
Gene Summary	Protein kinase which phosphorylates ESR1 (in vitro) and protects it against proteasomal degradation. May also regulate ESR1 levels indirectly via a PKC-AKT-FOXO3 pathway where it decreases the activity of PKC and the phosphorylation of AKT, thereby increasing binding of transcriptional activator FOXO3 to the ESR1 promoter and increasing ESR1 transcription (By similarity). Involved in endocytic trafficking of N-methyl-D-aspartate receptors (NMDAR) in neurons (PubMed:24760852).[UniProtKB/Swiss-Prot Function]

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