Erbin (NM_001289475) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	1700028E05Rik; Erbb2ip; mKIAA1225
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231794 representing NM_001289475 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTACAAAACGAAGTTTGTTTGTGCGGTTGGTACCATGTCGCTGTCTACGAGGAGAAGAGGAGACAG TCACTACTCTAGATTATTCTCATTGCAGCTTGGAACAAGTTCCCAAAGAGATTTTCACCTTTGAAAAAAC TTTAGAGGAGCTCTATTTAGATGCTAATCAGATTGAAGAGCTTCCAAAGCAACTTTTTAATTGTCAGTCT CTACATAAACTAAGTTTGCCAGACAATGATTTAACAACATTACCAGCCTCCATTGCAAATCTTATTAATC TCAGGGAACTGGATGTCAGCAAAAATGGAATACAAGAATTTCCAGAAAATATTAAAAATTGTAAAGTTTT GACAATTGTGGAGGCTAGTGTGAACCCTATTTCTAAGCTCCCTGATGGGTTTTCTCAGCTGCTAAACCTA ACCCAGTTATATCTGAATGATGCTTTCCTTGAATTCTTACCAGCTAATTTTGGCAGATTAACTAAACTCC AGATTCTAGAACTTAGAGAAAACCAGTTGAAAATGTTGCCTAAAACCATGAATAGACTGACCCAGCTGGA AAGGCTGGATTTGGGAAGTAATGAATTCACAGAAGTGCCTGAAGTACTTGAACAACTAAGTGGATTGAGA GAGTTTTGGATGGATGGCAATAGACTGACTTTTATTCCAGGGTTTATTGGTAGTTTGAGACAGCTCACGT ACTTGGATGTTTCTAAAAATAATATTGAAATGGTTGAAGAAGGAATTTCAACATGTGAAAACCTCCAGGA CTTTCTCTTATCAAGTAATTCCCTTCAGCAGCTGCCGGAAACTATTGGTTCATTGAAAAATGTCACAACT CTAAAAATTGATGAAAACCAATTAATGTATCTACCAGACTCAATAGGAGGGTTAAGGTCAATAGAAGAAC TGGATTGCAGTTTCAATGAAATTGAAGCTTTGCCTTCCTCAATTGGCCAGCTCACAAACATGAGAACATT TGCTGCAGATCACAATTACTTACAACAGCTACCCCCAGAGATTGGAAACTGGAAAAATATAACTGTGCTG TTTCTCCATTGTAATAAACTAGAGACGCTTCCAGAGGAAATGGGTGATATGCAAAAATTAAAAGTCATCA ATTTAAGTGATAATAGGTTAAAGAATTTGCCTTTTAGCTTTACAAAGCTACAACAGTTGACTGCTATGTG GCTGTCAGATAATCAGTCCAAACCTCTGATACCTCTTCAAAAAGAAACAGACACAGAGACTCAGAAAATG GTGCTTACTAATTACATGTTCCCTCAGCAGCCACGGACTGAAGACGTTATGTTCATATCAGATAATGAAA GTTTTAATCCTGCATTGTGGGAAGAACAGAGAAAACAGCGGGCTCAAGTGGCATTTGAATGTGATGAAGA CAAAGATGAAAGAGAGGCACCACCCAGGGAGGGAAACTTAAAGAGATATCCAACACCATACCCAGATGAG CTTAAGAATATGGTCAAGACTGTTCAAACCATTGTACATAGATTAAAAGATGAAGAGACTAATGAAGAGT CTGGGAGAGATTTGAAGCAGCATGAAGATCAGCAAGTTGTAAATAAGGATAAGTGTGTGAAGACTTCAGA AAGTACTACAACAAAAAGCAAACTTGATGAAAGAGAAAAGTATATGAATTCTGTACAGAAAATGAGTGAG CCTGAAGCTGAGACCAATGGTGGAAATTTGCCAGTAACTGCAAGTATGAAGTTGTCTGGAAACCTGAAGC ATATTGTTAACCATGATGATGTTTTTGAGGAATCTGAAGAACTTTCTTCTGATGAAGAAATGAAAATGGC AGAGATGCGACCTCCATTAATTGAATCCTCGATTAATCAGCCCAAAGTTGTAGCACTTAGTAATAATAAA AAGGATGATGCGAAGGATGCAGACTCTTTATCCGATGAAGTCACACACAACAGTAATCAGAATAACAGCA ACTGTTCTTCTCCGTCCCGAATGTCTGATTCAGTGTCTCTTAATACTGACAGCAGTCAAGACACGTCACT CTGTTCTCCCGTGAAACAGACTCCCGTGGATAGTAACTCCAAAGTCAGACAAGAAGATGAAAATTTTAAC AGCCTTTTACAAAATGGAGTTAATTTAAACAATTCACCAGAGGAAAAATTCAAAATTAATGATAAAAAGG ATTTTAAGTTACCTGAGTATGATTTGAATATTGAAGAACAGTTAGTTCTGATTGAAAAAGACATTGATTC AAAAGCCACATCTGATGACTCTCGCCAGTTAGACCATATCAACATGAATATTAATAAACTTGTGACCAAT AATATTTTTCAACCAGAAGTAATGGAAAGATCAAAGATGCAGGATATAGTACTTGGAACAGGCTTTTTAA GTATTCATCCTAAAAATGAGGCTGAGCACATAGAAAATGGGGCTAAGTTTCCGAATTTGGAGTCCATAAA TAAGGTAAATGGTCTTTGTGAGGACACTGCACCGTCTCCTGGTAGGGTTGAACCACAGAAGGCCAGTTCT TCTGCTGACGTGGGCATTTCTAAAAGCACGGAAGATCTATCTCCTCAGAGAAGTGGTCCAACTGGAGCTG TTGTGAAATCTCATAGTATAACTAACATGGAGACTGGAGGTTTAAAAATCTATGACATTCTTGGTGATGA TGGCCCTCAGCCGCCAAGTGCAGCAGTTAAAATCGCATCTGCTGTGGATGGGAAGAACATAGTCAGAAGC AAGTCTGCCACACTGCTGTATGATCAGCCCCTGCAGGTGTTCACTGCTGCCTCCTCATCTTCTGAGTTAC TGTCAGGTACAAAGGCAGTTTTCAAGTTTGATTCAAATCACAACCCTGAAGAGCCAGATATAATAAGAGC TGCCACAGTATCTGGCCCACAGTCTACACCTCACCTGTATGGTCCCCCACAATATAATGTCCAGTACAGT GGCAGTGCTACAGTGAAAGACACCTTGTGGCACCCTAAACAGAATCCACAAATAGATCCTGTCAGTTTTC CTCCTCAGCGCCTTCCAAGATCTGAGAGTGCAGAAAATCACAGTTATGCAAAGCATTCTGCCAACATGAA TTTCTCTAACCATAACAATGTTAGAGCCAATACTGGATACCACTTACAACAGAGACTTGCCCCAGCCAGG CATGGAGAAATGTGGGCTATCTCCCCGAATGACCGACTAGTGCCAGCAGTCACTCGGACTACTATTCAGA GACAGAGTAGCGTGTCTTCAACGGCTTCTGTAAATCTCGGTGACCCCACAAGGCGTACTGAAGGAGATTA CTTATCGTACAGAGAGTTACATTCAATGGGAAGAACTCCAGTCATGTCAGGATCACAGAGACCTCTTTCT GCACGAGCGTACAGCATCGATGGCCCAAATACATCCAGGCCTCAGAGTGCCCGTCCCTCTATTAATGAAA TACCAGAGAGAACTATGTCAGTTAGTGATTTCAATTACTCACGGACTAGTCCTTCAAAAAGACCAAATAC AAGGGTCGGGTCTGAACATTCTCTGTTAGATCCTCCAGGAAAAAGCAAGGTTCCTCATGACTGGCGGGAA CAAGTACTACGACACATTGAGGCCAAAAAGTTAGAAAAGAGTATGCTGTCAAGGTCCTTTAATTCCAATC TTACTGCTGTAAGCAGTTCTCACTATGGCAGCTCTAGGGATCTGCATGGCAGCCAGGGCAGCCTTGCCCT GAGTGTTGCAGACGGAAGAGGTTCTGGTGGGCACATTTTTCGAATGCCTTTGAGTAATGGACAAATGGGC CAGCCTCTCAGGCCTCAGGCACATTATAGTCAAACCCATCACCCCCCTCAGGCATCTGTGGCAAGGCATC CCTCTAGAGAACAACTAATTGATTATTTGATGCTGAAAGTGGCCCACCAGCCTCCATATACACATCCCCA TTGTTCTCCTAGACAAGGCCATGAACTGGCAAAGCAAGAGATTCGAGTAAGAGTAGAAAAGGATCCAGAA CTTGGATTTAGCATATCAGGAGGTGTCGGCGGCAGAGGAAACCCTTTCAGACCTGATGATGATGGTATAT TTGTAACAAGGGTACAACCTGAAGGACCAGCATCAAAATTACTACAGCCAGGAGATAAAATTATTCAGGC TAATGGCTACAGTTTTATCAACATTGAACATGGGCAAGCGGTGTCCTTGTTAAAAACTTTCCATAATGCA GTAGATCTCATCATTGTACGAGAAGTTTCTTCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231794 representing NM_001289475 Red=Cloning site Green=Tags(s) MTTKRSLFVRLVPCRCLRGEEETVTTLDYSHCSLEQVPKEIFTFEKTLEELYLDANQIEELPKQLFNCQS LHKLSLPDNDLTTLPASIANLINLRELDVSKNGIQEFPENIKNCKVLTIVEASVNPISKLPDGFSQLLNL TQLYLNDAFLEFLPANFGRLTKLQILELRENQLKMLPKTMNRLTQLERLDLGSNEFTEVPEVLEQLSGLR EFWMDGNRLTFIPGFIGSLRQLTYLDVSKNNIEMVEEGISTCENLQDFLLSSNSLQQLPETIGSLKNVTT LKIDENQLMYLPDSIGGLRSIEELDCSFNEIEALPSSIGQLTNMRTFAADHNYLQQLPPEIGNWKNITVL FLHCNKLETLPEEMGDMQKLKVINLSDNRLKNLPFSFTKLQQLTAMWLSDNQSKPLIPLQKETDTETQKM VLTNYMFPQQPRTEDVMFISDNESFNPALWEEQRKQRAQVAFECDEDKDEREAPPREGNLKRYPTPYPDE LKNMVKTVQTIVHRLKDEETNEESGRDLKQHEDQQVVNKDKCVKTSESTTTKSKLDEREKYMNSVQKMSE PEAETNGGNLPVTASMKLSGNLKHIVNHDDVFEESEELSSDEEMKMAEMRPPLIESSINQPKVVALSNNK KDDAKDADSLSDEVTHNSNQNNSNCSSPSRMSDSVSLNTDSSQDTSLCSPVKQTPVDSNSKVRQEDENFN SLLQNGVNLNNSPEEKFKINDKKDFKLPEYDLNIEEQLVLIEKDIDSKATSDDSRQLDHINMNINKLVTN NIFQPEVMERSKMQDIVLGTGFLSIHPKNEAEHIENGAKFPNLESINKVNGLCEDTAPSPGRVEPQKASS SADVGISKSTEDLSPQRSGPTGAVVKSHSITNMETGGLKIYDILGDDGPQPPSAAVKIASAVDGKNIVRS KSATLLYDQPLQVFTAASSSSELLSGTKAVFKFDSNHNPEEPDIIRAATVSGPQSTPHLYGPPQYNVQYS GSATVKDTLWHPKQNPQIDPVSFPPQRLPRSESAENHSYAKHSANMNFSNHNNVRANTGYHLQQRLAPAR HGEMWAISPNDRLVPAVTRTTIQRQSSVSSTASVNLGDPTRRTEGDYLSYRELHSMGRTPVMSGSQRPLS ARAYSIDGPNTSRPQSARPSINEIPERTMSVSDFNYSRTSPSKRPNTRVGSEHSLLDPPGKSKVPHDWRE QVLRHIEAKKLEKSMLSRSFNSNLTAVSSSHYGSSRDLHGSQGSLALSVADGRGSGGHIFRMPLSNGQMG QPLRPQAHYSQTHHPPQASVARHPSREQLIDYLMLKVAHQPPYTHPHCSPRQGHELAKQEIRVRVEKDPE LGFSISGGVGGRGNPFRPDDDGIFVTRVQPEGPASKLLQPGDKIIQANGYSFINIEHGQAVSLLKTFHNA VDLIIVREVSS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001289475
ORF Size	4233 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001289475.1, NP_001276404.1
RefSeq Size	6557 bp
RefSeq ORF	4236 bp
Locus ID	59079
UniProt ID	Q80TH2
MW	158.3 kDa
Gene Summary	Acts as an adapter for the receptor ERBB2, in epithelia. By binding the unphosphorylated ERBB2 'Tyr-1248' receptor, it may contribute to stabilize this unphosphorylated state (By similarity). Inhibits NOD2-dependent NF-kappa-B signaling and proinflammatory cytokine secretion (PubMed:16203728).[UniProtKB/Swiss-Prot Function]

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