Arhgef10l (NM_001290803) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	2810441C07Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231750 representing NM_001290803 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTCCTCGAACCCTCCTCCACAGCCTGCCATAGGAGCTCCCCTGGCTCCCAGCGCTCCAGGCCCCT CCCCCGAGGTGGAGGAGGACTCCGGAGAAGCCTTTGAGTTTGATGACAGTGATGAGGAGGAGGACACCAG CTCTGGCCTGGTTGTCCCTGGCCTCGCCCCTGAGAGGGACACAGAGCCCTCGCTGATCTGCTTTGACACT GTCCCGGGCTCAGACCTGGACCCAGCAGCTGCACCACCCCAGACAGAGGCACCCACTGTGGTCAGCAATG GGGATGCGGTGGGCGCAGCGATCTCTGGGGTCCGGCGCTCCAGCTGGAAGCGGAAGAGCTCACGTCGAAT TGACCGCTTCACTTTCCCTGCCCTGGAGGAAGATGTGATTTATGACGACGTCCCCTGTGAGAGCCCAGAC GCCCATCAGCCCGGGGCCGAGCGGGGCCTCGTTTACGAGGATGTGCATCGTGCAGGAGCACCGCGTGAGA CCGAGGACCTAGGCTGGAGCTCTAGTGAGTTTGAGAGCTACAGCGAGGACTCTGGGGAGGAGACCAAACC GGAGGCCGAGCCCACTAAGCACCGAGGGTCCTTCCAGCCCAAGCTTTCTCCAGACCTGACTAGGCTAAAG GAGAGATACGTCAGGACTAAGAGAGACATCTTGGCTTTGAGAGTTGGGGGTAGAGACATGCAGGAGCTGA AGCTCAAGTGCGATTGTAAGATGACCCAGCTCATGAAGGCCGCCAAGAGCGGGACCAGGGATGGGCTGGA GAAGACCCGGATGGCCGTCATGCGAAAAGTCTCCTTTCTGCATAGGAAAGATGTCCTCGGTGACTCAGAA GAAGAAGATATGGGGCTCCTGGAGGTTGGTGTGACAGACATCAAACCTCCCGCGCCAGAGCTGGGCCCCA TGCCGGATGGCTTGAGTCCTCAGCAGGTGGTCCGGAGACACATCCTAGGCTCCATCGTGCAGAGCGAAGG CAGCTACGTGGAGTCACTGAAGCGGATACTCCAGGATTACCGTAACCCACTAATGGAGATGGAGCCCAAG GCGCTGAGCGCCCGCAAGTGTCAGGTGGTGTTCTTCCGCGTGAAGGAGATTCTACATTGTCACTCCATGT TCCAGATCGCTCTGTCCTCCCGCGTGGCCGAGTGGGACTCCACAGAGAAGATTGGAGACCTCTTTGTGGC TTCATTCTCCAAGTCCATGGTCCTAGATGTATACAGCGACTATGTGAACAACTTCACCAACGCCATGTCC ATCATCAAGAAGGCCTGTCTCACCAAGCCAGCGTTCCTTGAGTTTCTCAAGCGGCGGCAGGTGTGCAGTA CGGACCGAGTCACCCTCTATGGGTTGATGGTGAAACCTGTTCAGAGATTTCCACAGTTCATCCTCCTACT CCAGGACATGCTGAAGAACACTCCCCGGGGCCACCCTGACAGGTTGTCGCTGCAGCTAGCCCTCACGGAG CTGGAGACGCTGGCTGAGAAGCTGAACGAGCAGAAGCGGTTGGCCGACCAGGTGGCTGAGATCCAGCAGC TGACCAAGAGTGTCAGTGACCGCAGCAGCCTCAACAAGCTGTTGACCTCTGGCCAGCGGCAGCTGCTTCT GTGTGAGACACTGACTGAGACGGTGTATGGAGACCGAGGGCAGCTGATCAAGTCCAAGGAGCGCAGGGTC TTCCTGCTCAATGACATGCTGGTCTGCGCCAACATCAACTTCAAGCCCTCCAACCACAGGGGCCAGCTAG AGATCAGCAGCCTGGTACCTCTGGGGCCCAAGTACGTGGTGAAGTGGAACACAGCTCTGCCTCAGGTGCA GGTGGTAGAGGTGGGTCAGGATGGTGGCACCTACGACAAAGACAATCTGCTCATCCAGCATGCTGGAGCC AAGAAGGCCACTGCTGCAGGGCAGGCCCAGAACAAGGTGTACCTGGGCCCCCCACGCCTCTTCCAGGAGC TGCAGGACCTGCAGAAGGACCTGGCCGTGGTGGAGCAGATCACCCTCCTCATCAGCACCTTGCATGGCAC TTACCAGAATCTGAACATGACTGTGGCCCAAGACTGGTGCCTGGCGCTGCAGAGGCTGATGCGGGTGAAG GAGGAGGAGATTCACTCGGCCAACAAGTGCCGCCTGAGGCTGCTGCTCCCCGGGAAGCCAGACAAGTCTG GCCGGCCCATCAGTTTCATGGTGGTCTTCATCACCCCGAACCCCCTGAGCAAGATTTCCTGGGTCAACAG ATTACACTTGGCAAAGATTGGACTAAGGGAGGAGAACCAGCCAGGATGGCTGTGTCCCGATGAGGACAAG AAAAGCAAAGCCCCGTTCTGGTGCCCCATCCTGGCCTGCTGCGTCCCCGCTTTCTCCTCCCGGACCCTCA GTCTGCAGCTTGGGGGCCTGGTCCACAGTCCTGTTAACTCTCCCCTGCTGGGCTTCTCGGCGGTCAGCAC TTCCCTTCCACAGGGCTACCTTTGGGTTGGCGGTGGTCAGGAGGGTGCTGGTGGCCAGGTGGAGATCTTC TCCCTGAACCGGCCTTCACCGCGCACGGTCAAGTCCTTCCCAGTGGCGGCCCCTGTGCTCTGCATAGAGT ATATTCCAGATCCGGAGGAGGAGGCTGAGGGTGCAGAGGAGAGCCGGGCAGCCACCGATCCCTCGGTCAC AGTGCATCCCACTGTCTGCCTTGGACTGCAGGATGGCAGCATCCTACTGTATGGCAGTGTGGACACGGGT ACCCAGTGCCTGGCCACCTGCAAGAGCCCAGGCCCGCAGCCTGTGCTCTGCCTGCGTCACAGCCCTTTCT ACCTGCTTGCCGGCCTCCAGGACGGGACCCTCGCTGCCTATCCTCGGACCAGTGGTGACATTCCCTGGGA CCTGGAGAGCCCTCCCATGTGCATAACTGTGGGCCCAGGGCCGATTCGAACACTGCTGAGCCTGGAAGAT GCTGCATGGGCCAGCTGCGGGCCGAGGGTCACTGTACTAGATGCCGCCACTTTGCAAACTCAGCAAAGCT TCGAGGCGCACCAGGATGAGGCGGTGAGTGTCACTCACATGGTGAAAGCCGGCAGCGGTGTCTGGATGGC CTTCTCCTCTGGCTCTTCCATCCGCCTCTTTCACACGGAGACTCTGGAGCACCTTCAGGAGATCAACATC GCCACCAGGACCACCTTCCTCCTGCCAGGCCAAAAGCACCTATGTGTCACCAGCCTCCTCATCTGCCAGG GTCTGCTCTGGGTAGGCACTGACCAGGGTGTCATTGTCCTGTTGCCTGTACCCCGACTGGAGGGCATCCC CAAGATCACAGGGAAAGGCATGGTGTCTCTCAATGGTCACTGTGGACCTGTGGCCTTCCTGGCTGTGGCC ATGAGCATCCTGGCCCCTGACATCCTGCGGAGTGACCAGGAAGAGGCCGAGGGACCGCAGGCCGAGGAGG ACAAGCCAGACGGGCAGGCTCATGAAACCGTGCCTGGGCCTGACAGCCACACAGCTCGGGAGCTGACCCG CAAGAAAGGCATCCTGCTCCAATACCGCCTACGCTCCACGGCCCACCTCCCCGGGCCCCTGTTGTCTGTG CGGGAGCCAGCACCTGCCGATGGCTCGGCTCTGGAACACAGTGAGGAGGACGGGTCCATCTACGAGATGG CCGATGACCCTGACGTCTGGGTCCGGAGCCGGCCCTGTGCCCGTGATGCCCACCGCAAGGAGATCTGCTC TGTGGCTATCATCTCTGGTGGACAAGGCTACCGCCATTTCGGCGGTGCTCCAGGTGGCCTGAGTGGGCGG GCGGCCCCGTGCAGCGAGACAGACAGCACACTTCTCATCTGGCAGGTGCCCTTGGCTCTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231750 representing NM_001290803 Red=Cloning site Green=Tags(s) MASSNPPPQPAIGAPLAPSAPGPSPEVEEDSGEAFEFDDSDEEEDTSSGLVVPGLAPERDTEPSLICFDT VPGSDLDPAAAPPQTEAPTVVSNGDAVGAAISGVRRSSWKRKSSRRIDRFTFPALEEDVIYDDVPCESPD AHQPGAERGLVYEDVHRAGAPRETEDLGWSSSEFESYSEDSGEETKPEAEPTKHRGSFQPKLSPDLTRLK ERYVRTKRDILALRVGGRDMQELKLKCDCKMTQLMKAAKSGTRDGLEKTRMAVMRKVSFLHRKDVLGDSE EEDMGLLEVGVTDIKPPAPELGPMPDGLSPQQVVRRHILGSIVQSEGSYVESLKRILQDYRNPLMEMEPK ALSARKCQVVFFRVKEILHCHSMFQIALSSRVAEWDSTEKIGDLFVASFSKSMVLDVYSDYVNNFTNAMS IIKKACLTKPAFLEFLKRRQVCSTDRVTLYGLMVKPVQRFPQFILLLQDMLKNTPRGHPDRLSLQLALTE LETLAEKLNEQKRLADQVAEIQQLTKSVSDRSSLNKLLTSGQRQLLLCETLTETVYGDRGQLIKSKERRV FLLNDMLVCANINFKPSNHRGQLEISSLVPLGPKYVVKWNTALPQVQVVEVGQDGGTYDKDNLLIQHAGA KKATAAGQAQNKVYLGPPRLFQELQDLQKDLAVVEQITLLISTLHGTYQNLNMTVAQDWCLALQRLMRVK EEEIHSANKCRLRLLLPGKPDKSGRPISFMVVFITPNPLSKISWVNRLHLAKIGLREENQPGWLCPDEDK KSKAPFWCPILACCVPAFSSRTLSLQLGGLVHSPVNSPLLGFSAVSTSLPQGYLWVGGGQEGAGGQVEIF SLNRPSPRTVKSFPVAAPVLCIEYIPDPEEEAEGAEESRAATDPSVTVHPTVCLGLQDGSILLYGSVDTG TQCLATCKSPGPQPVLCLRHSPFYLLAGLQDGTLAAYPRTSGDIPWDLESPPMCITVGPGPIRTLLSLED AAWASCGPRVTVLDAATLQTQQSFEAHQDEAVSVTHMVKAGSGVWMAFSSGSSIRLFHTETLEHLQEINI ATRTTFLLPGQKHLCVTSLLICQGLLWVGTDQGVIVLLPVPRLEGIPKITGKGMVSLNGHCGPVAFLAVA MSILAPDILRSDQEEAEGPQAEEDKPDGQAHETVPGPDSHTARELTRKKGILLQYRLRSTAHLPGPLLSV REPAPADGSALEHSEEDGSIYEMADDPDVWVRSRPCARDAHRKEICSVAIISGGQGYRHFGGAPGGLSGR AAPCSETDSTLLIWQVPLAL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001290803
ORF Size	3840 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001290803.1, NP_001277732.1
RefSeq Size	4549 bp
RefSeq ORF	3843 bp
Locus ID	72754
UniProt ID	A2AWP8
MW	140.4 kDa
Gene Summary	Acts as guanine nucleotide exchange factor (GEF) for RHOA, RHOB and RHOC.[UniProtKB/Swiss-Prot Function]

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