Arhgef10l (NM_001290803) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2810441C07Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229539 representing NM_001290803 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTCCTCGAACCCTCCTCCACAGCCTGCCATAGGAGCTCCCCTGGCTCCCAGCGCTCCAGGCCCCT CCCCCGAGGTGGAGGAGGACTCCGGAGAAGCCTTTGAGTTTGATGACAGTGATGAGGAGGAGGACACCAG CTCTGGCCTGGTTGTCCCTGGCCTCGCCCCTGAGAGGGACACAGAGCCCTCGCTGATCTGCTTTGACACT GTCCCGGGCTCAGACCTGGACCCAGCAGCTGCACCACCCCAGACAGAGGCACCCACTGTGGTCAGCAATG GGGATGCGGTGGGCGCAGCGATCTCTGGGGTCCGGCGCTCCAGCTGGAAGCGGAAGAGCTCACGTCGAAT TGACCGCTTCACTTTCCCTGCCCTGGAGGAAGATGTGATTTATGACGACGTCCCCTGTGAGAGCCCAGAC GCCCATCAGCCCGGGGCCGAGCGGGGCCTCGTTTACGAGGATGTGCATCGTGCAGGAGCACCGCGTGAGA CCGAGGACCTAGGCTGGAGCTCTAGTGAGTTTGAGAGCTACAGCGAGGACTCTGGGGAGGAGACCAAACC GGAGGCCGAGCCCACTAAGCACCGAGGGTCCTTCCAGCCCAAGCTTTCTCCAGACCTGACTAGGCTAAAG GAGAGATACGTCAGGACTAAGAGAGACATCTTGGCTTTGAGAGTTGGGGGTAGAGACATGCAGGAGCTGA AGCTCAAGTGCGATTGTAAGATGACCCAGCTCATGAAGGCCGCCAAGAGCGGGACCAGGGATGGGCTGGA GAAGACCCGGATGGCCGTCATGCGAAAAGTCTCCTTTCTGCATAGGAAAGATGTCCTCGGTGACTCAGAA GAAGAAGATATGGGGCTCCTGGAGGTTGGTGTGACAGACATCAAACCTCCCGCGCCAGAGCTGGGCCCCA TGCCGGATGGCTTGAGTCCTCAGCAGGTGGTCCGGAGACACATCCTAGGCTCCATCGTGCAGAGCGAAGG CAGCTACGTGGAGTCACTGAAGCGGATACTCCAGGATTACCGTAACCCACTAATGGAGATGGAGCCCAAG GCGCTGAGCGCCCGCAAGTGTCAGGTGGTGTTCTTCCGCGTGAAGGAGATTCTACATTGTCACTCCATGT TCCAGATCGCTCTGTCCTCCCGCGTGGCCGAGTGGGACTCCACAGAGAAGATTGGAGACCTCTTTGTGGC TTCATTCTCCAAGTCCATGGTCCTAGATGTATACAGCGACTATGTGAACAACTTCACCAACGCCATGTCC ATCATCAAGAAGGCCTGTCTCACCAAGCCAGCGTTCCTTGAGTTTCTCAAGCGGCGGCAGGTGTGCAGTA CGGACCGAGTCACCCTCTATGGGTTGATGGTGAAACCTGTTCAGAGATTTCCACAGTTCATCCTCCTACT CCAGGACATGCTGAAGAACACTCCCCGGGGCCACCCTGACAGGTTGTCGCTGCAGCTAGCCCTCACGGAG CTGGAGACGCTGGCTGAGAAGCTGAACGAGCAGAAGCGGTTGGCCGACCAGGTGGCTGAGATCCAGCAGC TGACCAAGAGTGTCAGTGACCGCAGCAGCCTCAACAAGCTGTTGACCTCTGGCCAGCGGCAGCTGCTTCT GTGTGAGACACTGACTGAGACGGTGTATGGAGACCGAGGGCAGCTGATCAAGTCCAAGGAGCGCAGGGTC TTCCTGCTCAATGACATGCTGGTCTGCGCCAACATCAACTTCAAGCCCTCCAACCACAGGGGCCAGCTAG AGATCAGCAGCCTGGTACCTCTGGGGCCCAAGTACGTGGTGAAGTGGAACACAGCTCTGCCTCAGGTGCA GGTGGTAGAGGTGGGTCAGGATGGTGGCACCTACGACAAAGACAATCTGCTCATCCAGCATGCTGGAGCC AAGAAGGCCACTGCTGCAGGGCAGGCCCAGAACAAGGTGTACCTGGGCCCCCCACGCCTCTTCCAGGAGC TGCAGGACCTGCAGAAGGACCTGGCCGTGGTGGAGCAGATCACCCTCCTCATCAGCACCTTGCATGGCAC TTACCAGAATCTGAACATGACTGTGGCCCAAGACTGGTGCCTGGCGCTGCAGAGGCTGATGCGGGTGAAG GAGGAGGAGATTCACTCGGCCAACAAGTGCCGCCTGAGGCTGCTGCTCCCCGGGAAGCCAGACAAGTCTG GCCGGCCCATCAGTTTCATGGTGGTCTTCATCACCCCGAACCCCCTGAGCAAGATTTCCTGGGTCAACAG ATTACACTTGGCAAAGATTGGACTAAGGGAGGAGAACCAGCCAGGATGGCTGTGTCCCGATGAGGACAAG AAAAGCAAAGCCCCGTTCTGGTGCCCCATCCTGGCCTGCTGCGTCCCCGCTTTCTCCTCCCGGACCCTCA GTCTGCAGCTTGGGGGCCTGGTCCACAGTCCTGTTAACTCTCCCCTGCTGGGCTTCTCGGCGGTCAGCAC TTCCCTTCCACAGGGCTACCTTTGGGTTGGCGGTGGTCAGGAGGGTGCTGGTGGCCAGGTGGAGATCTTC TCCCTGAACCGGCCTTCACCGCGCACGGTCAAGTCCTTCCCAGTGGCGGCCCCTGTGCTCTGCATAGAGT ATATTCCAGATCCGGAGGAGGAGGCTGAGGGTGCAGAGGAGAGCCGGGCAGCCACCGATCCCTCGGTCAC AGTGCATCCCACTGTCTGCCTTGGACTGCAGGATGGCAGCATCCTACTGTATGGCAGTGTGGACACGGGT ACCCAGTGCCTGGCCACCTGCAAGAGCCCAGGCCCGCAGCCTGTGCTCTGCCTGCGTCACAGCCCTTTCT ACCTGCTTGCCGGCCTCCAGGACGGGACCCTCGCTGCCTATCCTCGGACCAGTGGTGACATTCCCTGGGA CCTGGAGAGCCCTCCCATGTGCATAACTGTGGGCCCAGGGCCGATTCGAACACTGCTGAGCCTGGAAGAT GCTGCATGGGCCAGCTGCGGGCCGAGGGTCACTGTACTAGATGCCGCCACTTTGCAAACTCAGCAAAGCT TCGAGGCGCACCAGGATGAGGCGGTGAGTGTCACTCACATGGTGAAAGCCGGCAGCGGTGTCTGGATGGC CTTCTCCTCTGGCTCTTCCATCCGCCTCTTTCACACGGAGACTCTGGAGCACCTTCAGGAGATCAACATC GCCACCAGGACCACCTTCCTCCTGCCAGGCCAAAAGCACCTATGTGTCACCAGCCTCCTCATCTGCCAGG GTCTGCTCTGGGTAGGCACTGACCAGGGTGTCATTGTCCTGTTGCCTGTACCCCGACTGGAGGGCATCCC CAAGATCACAGGGAAAGGCATGGTGTCTCTCAATGGTCACTGTGGACCTGTGGCCTTCCTGGCTGTGGCC ATGAGCATCCTGGCCCCTGACATCCTGCGGAGTGACCAGGAAGAGGCCGAGGGACCGCAGGCCGAGGAGG ACAAGCCAGACGGGCAGGCTCATGAAACCGTGCCTGGGCCTGACAGCCACACAGCTCGGGAGCTGACCCG CAAGAAAGGCATCCTGCTCCAATACCGCCTACGCTCCACGGCCCACCTCCCCGGGCCCCTGTTGTCTGTG CGGGAGCCAGCACCTGCCGATGGCTCGGCTCTGGAACACAGTGAGGAGGACGGGTCCATCTACGAGATGG CCGATGACCCTGACGTCTGGGTCCGGAGCCGGCCCTGTGCCCGTGATGCCCACCGCAAGGAGATCTGCTC TGTGGCTATCATCTCTGGTGGACAAGGCTACCGCCATTTCGGCGGTGCTCCAGGTGGCCTGAGTGGGCGG GCGGCCCCGTGCAGCGAGACAGACAGCACACTTCTCATCTGGCAGGTGCCCTTGGCTCTATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001290803
Insert Size	3843 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001290803.1, NP_001277732.1
RefSeq Size	4549 bp
RefSeq ORF	3843 bp
Locus ID	72754
UniProt ID	A2AWP8
Gene Summary	Acts as guanine nucleotide exchange factor (GEF) for RHOA, RHOB and RHOC.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) represents the longest transcript and encodes the longest isoform (d).

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