Sorbs2 (NM_001205219) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	2010203O03Rik; 9430041O17Rik; A530071H08; Argbp2; mKIAA0777; nArgBP2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231691 representing NM_001205219 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATACAGATAGCGGTGGGTGTGCTCGCAAACGTGCCGCCATGTCTGTTACGTTAACATCTGTGAAGA GAGTTCAAAGTTCTCCAAACCTATTGGCTGCAGGGCGTGAGTCTCAGTCTCCAGATTCAGCTTGGAGATC TTACAATGATCGGAATCCAGAGACACTGAACGGAGATGCCACATATTCCTCTCTTGCAGCAAAAGGTTTT AGAAGCGTTCGGCCAAACCTGCAAGACAAAAGATCACCGACCCAGAGCCAGATCACTATCAATGGCAACT CTGGTGGCGCCGTGAGTCCAGTGAGTTACTATCAGAGGCCATTCTCCCCTTCTGCATACTCCCTCCCAGC CTCGCTGAACTCCAGCATTATCATGCAGCACGGCAGGTCTCTTGATTCTGCAGAGACATATTCCCAGCAT GCCCAGTCCCTGGATGGCACCATGGGAAGCTCCATCCCGCTCTATAGATCCTCCGAGGAAGAGAAGAGGG TCACGGTCATCAAAGCCCCGCATTACCCAGGGATCGGCCCTGTGGATGAGTCTGGAATCCCCACAGCCAT TAGGACGACTGTCGACAGGCCAAAGGACTGGTACAAGACAATGTTCAAGCAAATCCACATGGTACACAAG CCGGATGAGGACACAGACATGTATAATACTCCTTACACATACAACGCAGGTCTGTACAACTCGCCCTACA GTGCTCAGTCCCACCCGGCTGCGAAGACTCAGACCTACAGACCTCTTTCGAAAAGCCACTCAGACAATGG CACCGATGCGTTTAAGGAGGTGCCCTCCCCAGTGCCTCCCCCACACGTCCCACCACGACCAAGAGATCAG TCTTCAACACTAAAGCATGACTGGGACCCTCCAGACAGAAAGGTGGACACCAGGAAATTTCGATCGGAGC CAAGGAGTATTTTTGAATATGAGCCTGGGAAGTCGTCCATTCTGCAGCACGAAAGACCCCCTCCTAAAAA GGCTCTGGATTATGTTCAAGATCATTCTTCCGGTGTGTCCAACGAGGTCTCCATCTATCAGTCCTCCATA GACAGAAGCCTGGAAAGACCCAGCAGCTCTGCAAGCATGGCCGGTGACTTTAGGAAACGGAGGAAGAGCG AACCTGCAGTGGGCCCACTCCGGGGCTTGGGGGATCAGAGTTCAAGCAGGACCAGCCCTGGCCGGGCGGA CCTCCCAGGATCAAGTTCCACCTTTACGAAGTCTTTCATTAGCTCTTCTCCTTCCTCTCCCTCGAGAGCA CAAGGTGGGGATGATAGCAAAATGTGTCCGCCCCTTTGCAGTTACTCGGGGCTCAATGGCACCCCCTCTG GTGAGTTAGAGTGCTGCAACGCTTACAGACAACATTTGGACGTCCCCGGGGACTCGCAAAGGGCCATCAC TTTCAAGAATGGCTGGCAAATGGCCCGGCAAAATGCAGAGATCTGGAGTAGCACTGAAGAGACCGTTTCC CCCAAAATCAAATCACGTAGCTGCGATGATCTCCTGAATGACGACTGCGACAGCTTCCCAGACCCTAAAA CCAAGTCAGAGAGTATGGGTTCTCTGTTATGTGAAGAAGACTCCAAAGAGAGCTGCCCCATGACATGGGC GTCCCCCTACATCCAGGAAGTGTGCGGGAACAGCAGATCTAGGCTCAAACACAGGTCAGCCCATAATGCC CCAGGCTTCCTCAAAATGTACAAGAAAATGCATCGCATCAACCGCAAGGACTTGATGAATTCAGAGGTCA TTTGCTCGGTGAAATCCAGGATCCTGCAGTACGAGAAGGAGCAACAGCACAGGGGGCTGCTGCACGGATG GAGCCAGTCTTCCACCGAGGAGGTGCCCAGGGACGTGGTGCCCACTCGCATTTCGGAATTTGAAAAGCTG ATTCAGAAGTCGAAGTCTATGCCCAATCTAGGAGATGAAATGTTATCTCCTATAACCCTAGAACCCCCAC AAAATGGTTTGTGTCCCAAGAGGCGATTTTCTATCGAGTCTCTGCTGGAGGAAGAAACTCAGGTTCGTCA CCCTTCTCAGGGTCAGCGAAGCTGCAAGTCTAACACCCTGGTGCCCATCCACATCGAGGTCACCAGCGAC GAGCAACCTAGAACACACATGGAGTTTTCCGACAGTGACCAAGATGGGGTTGTGTCCGACCACAGCGACT ACGTTCACGTCGAAGGGTCGTCCTTTTGTAGTGAAAGTGACTTTGACCACTTTTCATTCACATCCTCTGA AAGCTTCTACGGATCGAGCCATCACCACCACCATCACCATCACCATCACCGACACCTCATCAGCTCCTGC AAAGGCCGATGCCCGGCTTCTTACACTCGATTTACCACAATGCTAAAACATGAAAGAGCTAAGCATGAAA ACATGGACCGACCCAGAAGGCAAGAAATGGATCCTGGCCTGTCTAAGCTTGCATTTCTAGTCAGCCCTGT GCCTTTCCGAAGGAAAAAAATCCTGACTCCCCAAAAACAGACTGAAAAGGCAAAATGCAAAGCCTCAGTA GTTGAAGCTCTGGACTCTGCCCTGAAAGACATTTGCGACCAAATAAAAGCCGAAAAGCGGAGGGGAAGCT TGCCAGACAACAGCATCCTGCACAGGCTTATCAGTGAACTGCTGCCACAGATTCCTGAAAGGAATTCATC TCTTCATGCTCTCAAAAGGAGCCCCATGCACCAGCCTTTCCACCCACTGCCTCCAGATGGTGCTAGTCAT TGTCCCCTGTACCAAAATGATTGTGGGAGAATGCCTCACAGTGCCTCTTTCCCTGACGTGGACACAACCA GCAACTACCACGCACAGGACTATGGGAGTGCACTGAGCCTCCAAGATCACGAGTCCCCTAGAAGTTACTC ATCTACTCTGACTGACTTGGGCAGAAGTGCATCACGGGAACGAAGGGGAACTCCAGAAAAAGAGAAATTG CCTGCAAAAGCTGTCTATGATTTCAAAGCTCAGACATCTAAGGAGCTGTCATTTAAGAAAGGAGACACTG TCTACATCCTCAGGAAAATTGACCAAAACTGGTATGAGGGGGAACACCACGGCAGAGTGGGCATTTTCCC AATCTCATACGTAGAGAAACTAACACCCCCGGAAAAAGCGCAGCCCGCAAGACCACCACCCCCAGTCCAG CCCGGAGAGATTGGAGAAGCCATAGCCAAGTACAACTTCAATGCAGACACAAATGTGGAACTCTCCCTGC GAAAGGGCGACAGGATTATTCTTCTTAAAAGAGTTGACCAAAACTGGTACGAAGGTAAAATCCCAGGAAC CAACAGACAAGGCATCTTCCCCGTGTCCTATGTCGAGGTTGTCAAGAGGAACGCAAAAGGTGCTGAGGAC TACCCAGACCCTCCTCTGCCCCACAGCTACTCCAGTGACAGAATTTACACCCTAAGCTCCAATAAGCCAC AGCGTCCTGGGTTCTCTCATGAAAACATTCAAGGTGGGGGAGAACCGTTTCAGGCTCTGTATAACTATAC TCCTAGGAACGAAGATGAGCTGGAACTCAGAGAAAGTGATGTCGTTGATGTCATGGAAAAGTGTGACGAC GGATGGTTTGTGGGAACTTCAAGAAGAACCAAATTCTTTGGTACTTTTCCCGGAAACTATGTCAAAAGGC TG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231691 representing NM_001205219 Red=Cloning site Green=Tags(s) MNTDSGGCARKRAAMSVTLTSVKRVQSSPNLLAAGRESQSPDSAWRSYNDRNPETLNGDATYSSLAAKGF RSVRPNLQDKRSPTQSQITINGNSGGAVSPVSYYQRPFSPSAYSLPASLNSSIIMQHGRSLDSAETYSQH AQSLDGTMGSSIPLYRSSEEEKRVTVIKAPHYPGIGPVDESGIPTAIRTTVDRPKDWYKTMFKQIHMVHK PDEDTDMYNTPYTYNAGLYNSPYSAQSHPAAKTQTYRPLSKSHSDNGTDAFKEVPSPVPPPHVPPRPRDQ SSTLKHDWDPPDRKVDTRKFRSEPRSIFEYEPGKSSILQHERPPPKKALDYVQDHSSGVSNEVSIYQSSI DRSLERPSSSASMAGDFRKRRKSEPAVGPLRGLGDQSSSRTSPGRADLPGSSSTFTKSFISSSPSSPSRA QGGDDSKMCPPLCSYSGLNGTPSGELECCNAYRQHLDVPGDSQRAITFKNGWQMARQNAEIWSSTEETVS PKIKSRSCDDLLNDDCDSFPDPKTKSESMGSLLCEEDSKESCPMTWASPYIQEVCGNSRSRLKHRSAHNA PGFLKMYKKMHRINRKDLMNSEVICSVKSRILQYEKEQQHRGLLHGWSQSSTEEVPRDVVPTRISEFEKL IQKSKSMPNLGDEMLSPITLEPPQNGLCPKRRFSIESLLEEETQVRHPSQGQRSCKSNTLVPIHIEVTSD EQPRTHMEFSDSDQDGVVSDHSDYVHVEGSSFCSESDFDHFSFTSSESFYGSSHHHHHHHHHHRHLISSC KGRCPASYTRFTTMLKHERAKHENMDRPRRQEMDPGLSKLAFLVSPVPFRRKKILTPQKQTEKAKCKASV VEALDSALKDICDQIKAEKRRGSLPDNSILHRLISELLPQIPERNSSLHALKRSPMHQPFHPLPPDGASH CPLYQNDCGRMPHSASFPDVDTTSNYHAQDYGSALSLQDHESPRSYSSTLTDLGRSASRERRGTPEKEKL PAKAVYDFKAQTSKELSFKKGDTVYILRKIDQNWYEGEHHGRVGIFPISYVEKLTPPEKAQPARPPPPVQ PGEIGEAIAKYNFNADTNVELSLRKGDRIILLKRVDQNWYEGKIPGTNRQGIFPVSYVEVVKRNAKGAED YPDPPLPHSYSSDRIYTLSSNKPQRPGFSHENIQGGGEPFQALYNYTPRNEDELELRESDVVDVMEKCDD GWFVGTSRRTKFFGTFPGNYVKRL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001205219
ORF Size	3642 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001205219.1, NP_001192148.1
RefSeq Size	6094 bp
RefSeq ORF	3645 bp
Locus ID	234214
MW	136.6 kDa
Gene Summary	Adapter protein that plays a role in the assembling of signaling complexes, being a link between ABL kinases and actin cytoskeleton. Can form complex with ABL1 and CBL, thus promoting ubiquitination and degradation of ABL1 (By similarity). May play a role in the regulation of pancreatic cell adhesion, possibly by acting on WASF1 phosphorylation, enhancing phosphorylation by ABL1, as well as dephosphorylation by PTPN12 (By similarity).[UniProtKB/Swiss-Prot Function]

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