Agbl1 (NM_001199224) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Ccp4; D430020F16; EG244071; Nna1-l1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231599 representing NM_001199224 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGAACAAGAAGGCAGTGGCCTGCAGATGCTGCTGCACACGCTCCAGAACTCCTCTGATAAAGCAT CCACCCTGTCCATCCTCCAAGTCCTCGGCGATCTGCTTTCTGTTGGCACAGACCGGAGGATTTATTATAT GATCAGCAAAGGTGGCAGCGAGGCCCTTCTGCAGACTCTGGTGGACACAGCAAGGTCATCTTCTCCTGAC TGGGACATCCTCCTGCCTCTCTTCAGGCTGCTGGCTAAAGTTGGCCTAAGAGATAAGAAGTTTGGACAGA AGGCACTAGAATTAGAAGCGCTTGATGTGACCTTGATTTTGGCCAGGAAAAACCTGTCTCACAGCCAGAA CCTCCTGCACTGTCTCTGGGTATTGCGTGTGTTTGCCTCCAGTGTAACCACGGGAGCCATGCTGGGAATT AATGGAGCGATGGAACTGCTCTTCAAGGTCCTTTCTCCTTACACCCGAAAGCACACCCGGACAATCAGGG CAGCCACTGAAGTTTTGGCAGCATTGCTGAAATCCAAGTCTAACTGCCGCAGGGCAGTGAATAGAGGCTA TGTCAACAGCTTGCTCAGGCTGCATCAGGACTGGCACAGCCGCGATGTGACCAATACCTATGTGACGATC CGCCATGGGCTTCTGCTCTGCCTCAGGCACATTGTCGCCCTCCGCTCTGGCAGGGAGGCCTTCTTGGCAG CCCAAGGCATGGAGACTCTCTTCAGCATTGCACAGACCTGCCTGGAGAACAAGAACATGGAACTTGTGAT CTCTGCTGTGATCCAGATCCTCAGACAGTGTTACCCTGCGAGTCGGCTTCCCCTGGTCACAGCCAGCAGT GCCTATACCTTCCCAGCCCCTGGGAGCACCAGCTCAGAGCTCCCACTCAATCTGACTGAAGAAGATTTTG ACGATGATGGTGATGAAGAGATGGACAAAGACTCAGATGTGGAGGCTGTGAAAGAAGATGATGACTTGGA AACTGATCTGAGCAAACTTAGTTCTAAGCCTGGTCTTGACCTCCCTGAGGAGGAACTTGCACAGTATGAT GCAATGTGTCCTGAGCTGTCCTGTAGCTTTGAGGAACTGGAACCCAAATGTGGAGATGATTTGAACAACA AAGACACTCTGCATGCCAATCACCACCACATTCCAAGTGTTGCTTCTCTCAGGCAACACTGTTTTAACCG GGAGCACAGCTCCTGGAGACAAGAAAGAGAAGACACAGTCCACTCGTCCATTCTGCACATGGTGAAGACA GGGAAGTCCGGTGTACCGTCATCCTCAAAACAAAGATCTGCAACAAATGTGAATCAAAGCCTACAACAAA ATGGTTTGGAGATCGATTCTTCTGGACATGATACCTCTGACATTCAGGCTCCCTTAGAACAGGCTGCTTG GGACATGGAAGCAATTTCTTGTCCAAGGATAACTGCTTCATTTCCCAATTCTACCAAGCCAGAAGAAAGC ATCGGAGCAGCAGAGAAACTTCTGCACACACATGCCAAGCACATACCTTTCCACGATCCTCATCTTTATA TAGCCAATGCTATGAGAACAAGATCTGCTGTGGGATTCAAGACAATGGCATTTCCTGATCTCTGGGGCCA CTGTCCACCTCCCGCTGCTCAACCCATGCTGGATCGCAAACTGGGAGTACAGAGGATCAAGATACTTGAG GATATCCGGAGGCTCCTCCATCCAAGTGATGTTATAAACAAAGTTGTCTTCAGTTTAGACGAGCCCAGGC CTTTGCAAGGCAGCATTTCCAATTGCTTAATGTTCCACTCCAAGTTTGAATCAGGAAATCTCCGTAAAGC CATCCAAGTGCGTGAGTTTGAGTATGACTTGTTGGTCAATGCCGACGTGAATAGCTCCCAGCACCAGCAG TGGTTCTACTTCAAGGTGAGCGGCATGAGGGCCGCTGTCCCTTATCACTTCAACATCATCAATTGTGAGA AACCCAACAGCCAGTTCAACTATGGGATGCAGCCAACCCTGTATTCTGTGAAAGAGGCTCTTCTTGGTCG ACCTGCCTGGATACGGACAGGCTCTGACATTTGTTACTACAAAAATCACTACCGTCAGAATGCAGCTACC ATGGATGGAGCATTGGGGAAGCGCTACTACACCCTTACCTTTGCTGTTACCTTCCCTCATAATGAGGATG CCTGCTATCTGGCCTATCACTATCCCTACACATATTCCACCCTCATGACTCATCTTGAAATTCTGGAAAG AAGCATCGACCACAGGGAAATCTACTTCCGACATGATGTTCTCTGCCAAACGTTGGGAGGGAATCCATGT CCGCTGGTGACAATCACTGCCTTTCCTGAATCTAACAGCACCGAGCATCTGGAGCAGTTCCGCTGTCGTC CATATCAGGTGATCACAGCCCGAGTTCACCCAGGAGAGAGCAATGCCAGCTGGGTGATGAAGGGAACCTT GGAGTTCCTAGTCAGCAGTGACCCCGTGGCAAAGCTCCTGAGGGAAAACTTCGTGTTCAAGATCATCCCT ATGCTCAACCCAGATGGTGTCATCAATGGCAATCACAGATGCTCACTGAGAGGCGAGGATCTCAACAGAC AGTGGCTCTCTCCCCAAGCTCATCTCCAACCAACCATCTACCATGCTAAAGGTCTCCTCCATTACCTAAG CAGTACTGGCCGGGGACCTGTGGTCTTCTGTGATTTCCATGGTCACTCCCAAAAGAAGAATGTATTCCTT TATGGTTGCAGCATGAAAGAAACTTTGTGGCAAGCAGGCTGTACTGTGGGTGAATCTGCCCTCTTGGAGG ATGTCAGCTACAGGACACTTCCAAAAATCCTGGACAAGCTAGCACCAGCATTCACCATGAACAGCTGCAG CTTTCTGGTTGAAAAATCACGAGCTTCCACTGCCCGAGTGGTGGTGTGGAGAGAGATGGGGGTTTCCAGA AGCTATACCATGGAGAGCAGCTACTGTGGCTGCAACCAGGGCCCCTATCAGGGTCTACAGTTTGGTACTG GAGAACTAGAAGAAATGGGAGCCATGTACTGCTTGGGACTGCTCATCTTGGAACTCAAATCTGTAAACTG TAGCCATAAGCTCTTGGCTCGAGCTTCATCTCTGCTGAACGCGGATGTTCTGGAACACTACCTCCAGCGG TGCAGTAGCAGCAGCAGCAACAGCAGCAACAGAACCTCTGAGGTAGATGATGAGCCATATTGCATGGAGG AGATTGACTACAGTGCTGACAGCAGCTCAGACGCAGAACAGAACTTCACTGAACTGGACCGGCAGATCCA GGAGTGTGCCTTGAACAAGGATGAAGAAGAAGAAGAAAAAGAGGAAGGAACAGGATGGAGAAGAAGATCT GTCACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231599 representing NM_001199224 Red=Cloning site Green=Tags(s) MAEQEGSGLQMLLHTLQNSSDKASTLSILQVLGDLLSVGTDRRIYYMISKGGSEALLQTLVDTARSSSPD WDILLPLFRLLAKVGLRDKKFGQKALELEALDVTLILARKNLSHSQNLLHCLWVLRVFASSVTTGAMLGI NGAMELLFKVLSPYTRKHTRTIRAATEVLAALLKSKSNCRRAVNRGYVNSLLRLHQDWHSRDVTNTYVTI RHGLLLCLRHIVALRSGREAFLAAQGMETLFSIAQTCLENKNMELVISAVIQILRQCYPASRLPLVTASS AYTFPAPGSTSSELPLNLTEEDFDDDGDEEMDKDSDVEAVKEDDDLETDLSKLSSKPGLDLPEEELAQYD AMCPELSCSFEELEPKCGDDLNNKDTLHANHHHIPSVASLRQHCFNREHSSWRQEREDTVHSSILHMVKT GKSGVPSSSKQRSATNVNQSLQQNGLEIDSSGHDTSDIQAPLEQAAWDMEAISCPRITASFPNSTKPEES IGAAEKLLHTHAKHIPFHDPHLYIANAMRTRSAVGFKTMAFPDLWGHCPPPAAQPMLDRKLGVQRIKILE DIRRLLHPSDVINKVVFSLDEPRPLQGSISNCLMFHSKFESGNLRKAIQVREFEYDLLVNADVNSSQHQQ WFYFKVSGMRAAVPYHFNIINCEKPNSQFNYGMQPTLYSVKEALLGRPAWIRTGSDICYYKNHYRQNAAT MDGALGKRYYTLTFAVTFPHNEDACYLAYHYPYTYSTLMTHLEILERSIDHREIYFRHDVLCQTLGGNPC PLVTITAFPESNSTEHLEQFRCRPYQVITARVHPGESNASWVMKGTLEFLVSSDPVAKLLRENFVFKIIP MLNPDGVINGNHRCSLRGEDLNRQWLSPQAHLQPTIYHAKGLLHYLSSTGRGPVVFCDFHGHSQKKNVFL YGCSMKETLWQAGCTVGESALLEDVSYRTLPKILDKLAPAFTMNSCSFLVEKSRASTARVVVWREMGVSR SYTMESSYCGCNQGPYQGLQFGTGELEEMGAMYCLGLLILELKSVNCSHKLLARASSLLNADVLEHYLQR CSSSSSNSSNRTSEVDDEPYCMEEIDYSADSSSDAEQNFTELDRQIQECALNKDEEEEEKEEGTGWRRRS VT TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001199224
ORF Size	3366 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001199224.1, NP_001186153.1
RefSeq Size	3369 bp
RefSeq ORF	3369 bp
Locus ID	244071
UniProt ID	Q09M05
MW	126.6 kDa
Gene Summary	Metallocarboxypeptidase that mediates deglutamylation of target proteins. Catalyzes the deglutamylation of polyglutamate side chains generated by post-translational polyglutamylation in proteins such as tubulins. Also removes gene-encoded polyglutamates from the carboxy-terminus of target proteins such as MYLK. Acts as a long-chain deglutamylase and specifically shortens long polyglutamate chains, while it is not able to remove the branching point glutamate, a process catalyzed by AGBL5/CCP5.[UniProtKB/Swiss-Prot Function]

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