Agbl1 (NM_001199224) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Ccp4; D430020F16; EG244071; Nna1-l1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229388 representing NM_001199224 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGAACAAGAAGGCAGTGGCCTGCAGATGCTGCTGCACACGCTCCAGAACTCCTCTGATAAAGCAT CCACCCTGTCCATCCTCCAAGTCCTCGGCGATCTGCTTTCTGTTGGCACAGACCGGAGGATTTATTATAT GATCAGCAAAGGTGGCAGCGAGGCCCTTCTGCAGACTCTGGTGGACACAGCAAGGTCATCTTCTCCTGAC TGGGACATCCTCCTGCCTCTCTTCAGGCTGCTGGCTAAAGTTGGCCTAAGAGATAAGAAGTTTGGACAGA AGGCACTAGAATTAGAAGCGCTTGATGTGACCTTGATTTTGGCCAGGAAAAACCTGTCTCACAGCCAGAA CCTCCTGCACTGTCTCTGGGTATTGCGTGTGTTTGCCTCCAGTGTAACCACGGGAGCCATGCTGGGAATT AATGGAGCGATGGAACTGCTCTTCAAGGTCCTTTCTCCTTACACCCGAAAGCACACCCGGACAATCAGGG CAGCCACTGAAGTTTTGGCAGCATTGCTGAAATCCAAGTCTAACTGCCGCAGGGCAGTGAATAGAGGCTA TGTCAACAGCTTGCTCAGGCTGCATCAGGACTGGCACAGCCGCGATGTGACCAATACCTATGTGACGATC CGCCATGGGCTTCTGCTCTGCCTCAGGCACATTGTCGCCCTCCGCTCTGGCAGGGAGGCCTTCTTGGCAG CCCAAGGCATGGAGACTCTCTTCAGCATTGCACAGACCTGCCTGGAGAACAAGAACATGGAACTTGTGAT CTCTGCTGTGATCCAGATCCTCAGACAGTGTTACCCTGCGAGTCGGCTTCCCCTGGTCACAGCCAGCAGT GCCTATACCTTCCCAGCCCCTGGGAGCACCAGCTCAGAGCTCCCACTCAATCTGACTGAAGAAGATTTTG ACGATGATGGTGATGAAGAGATGGACAAAGACTCAGATGTGGAGGCTGTGAAAGAAGATGATGACTTGGA AACTGATCTGAGCAAACTTAGTTCTAAGCCTGGTCTTGACCTCCCTGAGGAGGAACTTGCACAGTATGAT GCAATGTGTCCTGAGCTGTCCTGTAGCTTTGAGGAACTGGAACCCAAATGTGGAGATGATTTGAACAACA AAGACACTCTGCATGCCAATCACCACCACATTCCAAGTGTTGCTTCTCTCAGGCAACACTGTTTTAACCG GGAGCACAGCTCCTGGAGACAAGAAAGAGAAGACACAGTCCACTCGTCCATTCTGCACATGGTGAAGACA GGGAAGTCCGGTGTACCGTCATCCTCAAAACAAAGATCTGCAACAAATGTGAATCAAAGCCTACAACAAA ATGGTTTGGAGATCGATTCTTCTGGACATGATACCTCTGACATTCAGGCTCCCTTAGAACAGGCTGCTTG GGACATGGAAGCAATTTCTTGTCCAAGGATAACTGCTTCATTTCCCAATTCTACCAAGCCAGAAGAAAGC ATCGGAGCAGCAGAGAAACTTCTGCACACACATGCCAAGCACATACCTTTCCACGATCCTCATCTTTATA TAGCCAATGCTATGAGAACAAGATCTGCTGTGGGATTCAAGACAATGGCATTTCCTGATCTCTGGGGCCA CTGTCCACCTCCCGCTGCTCAACCCATGCTGGATCGCAAACTGGGAGTACAGAGGATCAAGATACTTGAG GATATCCGGAGGCTCCTCCATCCAAGTGATGTTATAAACAAAGTTGTCTTCAGTTTAGACGAGCCCAGGC CTTTGCAAGGCAGCATTTCCAATTGCTTAATGTTCCACTCCAAGTTTGAATCAGGAAATCTCCGTAAAGC CATCCAAGTGCGTGAGTTTGAGTATGACTTGTTGGTCAATGCCGACGTGAATAGCTCCCAGCACCAGCAG TGGTTCTACTTCAAGGTGAGCGGCATGAGGGCCGCTGTCCCTTATCACTTCAACATCATCAATTGTGAGA AACCCAACAGCCAGTTCAACTATGGGATGCAGCCAACCCTGTATTCTGTGAAAGAGGCTCTTCTTGGTCG ACCTGCCTGGATACGGACAGGCTCTGACATTTGTTACTACAAAAATCACTACCGTCAGAATGCAGCTACC ATGGATGGAGCATTGGGGAAGCGCTACTACACCCTTACCTTTGCTGTTACCTTCCCTCATAATGAGGATG CCTGCTATCTGGCCTATCACTATCCCTACACATATTCCACCCTCATGACTCATCTTGAAATTCTGGAAAG AAGCATCGACCACAGGGAAATCTACTTCCGACATGATGTTCTCTGCCAAACGTTGGGAGGGAATCCATGT CCGCTGGTGACAATCACTGCCTTTCCTGAATCTAACAGCACCGAGCATCTGGAGCAGTTCCGCTGTCGTC CATATCAGGTGATCACAGCCCGAGTTCACCCAGGAGAGAGCAATGCCAGCTGGGTGATGAAGGGAACCTT GGAGTTCCTAGTCAGCAGTGACCCCGTGGCAAAGCTCCTGAGGGAAAACTTCGTGTTCAAGATCATCCCT ATGCTCAACCCAGATGGTGTCATCAATGGCAATCACAGATGCTCACTGAGAGGCGAGGATCTCAACAGAC AGTGGCTCTCTCCCCAAGCTCATCTCCAACCAACCATCTACCATGCTAAAGGTCTCCTCCATTACCTAAG CAGTACTGGCCGGGGACCTGTGGTCTTCTGTGATTTCCATGGTCACTCCCAAAAGAAGAATGTATTCCTT TATGGTTGCAGCATGAAAGAAACTTTGTGGCAAGCAGGCTGTACTGTGGGTGAATCTGCCCTCTTGGAGG ATGTCAGCTACAGGACACTTCCAAAAATCCTGGACAAGCTAGCACCAGCATTCACCATGAACAGCTGCAG CTTTCTGGTTGAAAAATCACGAGCTTCCACTGCCCGAGTGGTGGTGTGGAGAGAGATGGGGGTTTCCAGA AGCTATACCATGGAGAGCAGCTACTGTGGCTGCAACCAGGGCCCCTATCAGGGTCTACAGTTTGGTACTG GAGAACTAGAAGAAATGGGAGCCATGTACTGCTTGGGACTGCTCATCTTGGAACTCAAATCTGTAAACTG TAGCCATAAGCTCTTGGCTCGAGCTTCATCTCTGCTGAACGCGGATGTTCTGGAACACTACCTCCAGCGG TGCAGTAGCAGCAGCAGCAACAGCAGCAACAGAACCTCTGAGGTAGATGATGAGCCATATTGCATGGAGG AGATTGACTACAGTGCTGACAGCAGCTCAGACGCAGAACAGAACTTCACTGAACTGGACCGGCAGATCCA GGAGTGTGCCTTGAACAAGGATGAAGAAGAAGAAGAAAAAGAGGAAGGAACAGGATGGAGAAGAAGATCT GTCACCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001199224
Insert Size	3369 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001199224.1, NP_001186153.1
RefSeq Size	3369 bp
RefSeq ORF	3369 bp
Locus ID	244071
UniProt ID	Q09M05
Gene Summary	Metallocarboxypeptidase that mediates deglutamylation of target proteins. Catalyzes the deglutamylation of polyglutamate side chains generated by post-translational polyglutamylation in proteins such as tubulins. Also removes gene-encoded polyglutamates from the carboxy-terminus of target proteins such as MYLK. Acts as a long-chain deglutamylase and specifically shortens long polyglutamate chains, while it is not able to remove the branching point glutamate, a process catalyzed by AGBL5/CCP5.[UniProtKB/Swiss-Prot Function]

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