Hdac5 (NM_001284248) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AI426555; Hdac4; mHDA1; mKIAA0600
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231598 representing NM_001284248 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCTGGTGCCCAAGGCACAGGGGCTTGTGGAGATGCTGCAGACCATCTATGAGACCGAGTCCTGTT TCTCAGCAGATGGCATGTCAGGCCGGGAACCATCCTTGGAAATCCTGCCACGGACTCCTCTGCACAGCAT CCCTGTGGCAGTGGAGGTGAAGCCGGTGCTGCCAGGAGCCATGCCCAGCTCCATGGGGGGTGGAGGTGGA GGTAGCCCCAGCCCCGTGGAGCTTCGGGGGGCTCTGGCGGGCCCCATGGACCCTGCGCTACGGGAGCAGC AACTGCAGCAGGAGCTCCTGGTCCTCAAGCAGCAGCAGCAGCTCCAGAAGCAGCTCCTGTTCGCCGAGTT CCAGAAGCAGCACGACCACTTGACGCGGCAGCACGAGGTCCAGCTGCAGAAGCACCTCAAGCAGCAGCAG GAGATGCTGGCGGCTAAGAGGCAGCAGGAGCTGGAGCAGCAGCGGCAGCGGGAGCAGCAGCGGCAGGAGG AGCTGGAGAAACAGCGGCTGGAGCAGCAGCTGCTCATCCTGCGCAACAAGGAGAAGAGCAAAGAGAGTGC CATCGCCAGCACCGAGGTAAAGCTGAGGCTCCAGGAATTCCTGTTGTCCAAGTCAAAGGAGCCCACGCCA GGCGGCCTCAACCATTCCCTCCCACAGCACCCCAAATGCTGGGGAGCCCACCACGCTTCTTTGGACCAGA GTTCCCCTCCCCAGAGCGGCCCTCCTGGGACGCCTCCCTCCTACAAATTGCCTTTGCTTGGGCCCTATGA CAGCCGTGATGACTTTCCCCTCCGTAAAACGGCCTCGGAACCCAACTTAAAAGTACGTTCGAGGCTAAAA CAGAAGGTAGCCGAGAGGAGAAGCAGTCCCCTCCTGCGTCGAAAGGATGGCACTGTTATTAGTACTTTTA AGAAGAGAGCAGTTGAGATCACCGGCACGGGGCCTGGGGTGTCGTCCGTGTGTAACAGTGCGCCCGGCTC TGGCCCCAGCTCTCCCAACAGTTCCCACAGCACCATCGCTGAGAACGGCTTTACTGGCTCAGTCCCCAAC ATCCCCACTGAGATGATCCCCCAGCACCGGGCCCTCCCTCTGGACAGTTCCCCAAACCAGTTCAGCCTCT ATACGTCTCCTTCTCTGCCCAACATCTCCCTAGGGCTGCAGGCCACTGTCACTGTCACCAACTCGCACCT CACCGCCTCCCCGAAGCTGTCAACACAGCAGGAGGCTGAGAGGCAGGCCCTTCAGTCCCTGCGGCAGGGC GGCACACTGACCGGCAAGTTCATGAGCACATCCTCCATCCCTGGCTGCCTGTTGGGAGTGGCACTGGAGG GTGACACAAGCCCCCACGGGCACGCTTCCCTGCTGCAGCACGTTTTGCTCCTGGAGCAGGCCCGGCAACA GAGCACGCTCATAGCAGTGCCGCTCCATGGGCAGTCCCCACTGGTGACGGGTGAACGTGTGGCCACCAGC ATGAGGACGGTGGGTAAGCTCCCGAGGCACCGACCTCTGAGCCGCACTCAGTCCTCCCCGCTGCCGCAGA GTCCCCAGGCCCTGCAGCAGCTGGTCATGCAGCAGCAGCACCAGCAGTTCCTGGAGAAGCAGAAGCAGCA GCAGATGCAGCTGGGCAAGATCCTTACCAAAACTGGGGAGCTGTCAAGGCAGCCCACCACTCACCCGGAG GAGACAGAAGAGGAGCTGACGGAGCAGCAGGAGGCCTTGCTGGGAGAGGGGGCCCTGACCATTCCCCGGG AAGGCTCTACAGAAAGTGAGAGCACCCAGGAAGACCTAGAAGAGGAGGAGGAGGAGGAGGAGGAGGAAGA GGAGGACTGCATTCAGGTCAAGGATGAGGATGGCGAGAGTGGTCCTGATGAAGGCCCTGACTTAGAAGAG TCCAGTGCTGGTTACAAAAAGTTGTTCGCAGATGCCCAGCAGTTACAGCCCCTCCAGGTGTACCAGGCAC CCCTCAGCCTGGCCACTGTGCCTCATCAGGCCCTGGGCCGCACCCAGTCCTCACCTGCTGCTCCTGGGAG CATGAAGAGCCCCACAGACCAACCCACTGTGGTGAAGCACCTCTTCACCACAGGTGTGGTCTATGACACG TTCATGCTGAAGCACCAGTGTATGTGCGGAAACACACACGTGCACCCAGAGCACGCCGGCCGCATCCAGA GCATCTGGTCCCGGCTGCAGGAAACTGGTCTGCTCGGCAAGTGTGAGCGGATCCGGGGTCGTAAAGCCAC ACTGGATGAAATCCAGACCGTGCACTCTGAGTACCACACCCTGCTCTATGGGACCAGCCCCCTTAACCGG CAGAAGCTGGACAGCAAGAAGCTGCTTGGCCCCATCAGCCAGAAGATGTACGCCATGCTGCCCTGTGGGG GCATTGGGGTGGACAGTGACACGGTGTGGAATGAGATGCACTCCTCAAGTGCCGTGCGAATGGCAGTGGG CTGCCTGGTGGAGCTGGCCTTCAAGGTGGCTGCAGGAGAGCTCAAGAATGGATTTGCTATCATCCGGCCC CCAGGACACCATGCTGAGGAGTCCACAGCCATGGGATTCTGCTTCTTCAACTCCGTAGCCATCACAGCTA AACTCCTGCAGCAGAAGCTGAGCGTGGGCAAGGTCCTCATCGTGGACTGGGATATTCACCATGGCAACGG CACCCAGCAAGCATTCTACAACGATCCCTCTGTGCTCTACATCTCCCTGCATCGCTACGACAACGGGAAC TTCTTTCCAGGCTCTGGGGCTCCTGAAGAGGTTGGTGGAGGGCCAGGTGTGGGGTACAACGTAAATGTGG CGTGGACAGGAGGTGTGGATCCCCCCATTGGAGATGTGGAATACCTGACAGCCTTCAGGACAGTGGTGAT GCCCATTGCCCAGGAGTTCTCACCTGACGTCGTCCTAGTCTCCGCTGGGTTTGATGCTGTTGAAGGACAT CTGTCTCCACTGGGTGGCTATTCTGTCACCGCCAGATGTTTTGGCCACTTGACCAGGCAGCTCATGACAC TGGCTGGGGGCCGGGTGGTGCTGGCCCTGGAGGGAGGCCATGACTTGACCGCCATCTGTGATGCCTCTGA GGCCTGTGTCTCGGCTCTGCTCAGCGTGGAGCTGCAGCCCTTGGATGAAGCAGTCTTGCAGCAAAAGCCC AGCGTCAATGCGGTTGCCACACTAGAGAAAGTCATCGAGATCCAGAGCAAACACTGGAGCTGTGTACAGA GGTTTGCCGCTGGTCTGGGCTGCTCGCTGCGGGAGGCTCAGACAGGTGAGAAAGAGGAGGCCGAGACTGT GAGCGCCATGGCCCTGCTTTCCGTGGGGGCTGAGCAGGCCCAGGCTGTTGCCACTCAAGAGCACAGCCCC AGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231598 representing NM_001284248 Red=Cloning site Green=Tags(s) MLLVPKAQGLVEMLQTIYETESCFSADGMSGREPSLEILPRTPLHSIPVAVEVKPVLPGAMPSSMGGGGG GSPSPVELRGALAGPMDPALREQQLQQELLVLKQQQQLQKQLLFAEFQKQHDHLTRQHEVQLQKHLKQQQ EMLAAKRQQELEQQRQREQQRQEELEKQRLEQQLLILRNKEKSKESAIASTEVKLRLQEFLLSKSKEPTP GGLNHSLPQHPKCWGAHHASLDQSSPPQSGPPGTPPSYKLPLLGPYDSRDDFPLRKTASEPNLKVRSRLK QKVAERRSSPLLRRKDGTVISTFKKRAVEITGTGPGVSSVCNSAPGSGPSSPNSSHSTIAENGFTGSVPN IPTEMIPQHRALPLDSSPNQFSLYTSPSLPNISLGLQATVTVTNSHLTASPKLSTQQEAERQALQSLRQG GTLTGKFMSTSSIPGCLLGVALEGDTSPHGHASLLQHVLLLEQARQQSTLIAVPLHGQSPLVTGERVATS MRTVGKLPRHRPLSRTQSSPLPQSPQALQQLVMQQQHQQFLEKQKQQQMQLGKILTKTGELSRQPTTHPE ETEEELTEQQEALLGEGALTIPREGSTESESTQEDLEEEEEEEEEEEEDCIQVKDEDGESGPDEGPDLEE SSAGYKKLFADAQQLQPLQVYQAPLSLATVPHQALGRTQSSPAAPGSMKSPTDQPTVVKHLFTTGVVYDT FMLKHQCMCGNTHVHPEHAGRIQSIWSRLQETGLLGKCERIRGRKATLDEIQTVHSEYHTLLYGTSPLNR QKLDSKKLLGPISQKMYAMLPCGGIGVDSDTVWNEMHSSSAVRMAVGCLVELAFKVAAGELKNGFAIIRP PGHHAEESTAMGFCFFNSVAITAKLLQQKLSVGKVLIVDWDIHHGNGTQQAFYNDPSVLYISLHRYDNGN FFPGSGAPEEVGGGPGVGYNVNVAWTGGVDPPIGDVEYLTAFRTVVMPIAQEFSPDVVLVSAGFDAVEGH LSPLGGYSVTARCFGHLTRQLMTLAGGRVVLALEGGHDLTAICDASEACVSALLSVELQPLDEAVLQQKP SVNAVATLEKVIEIQSKHWSCVQRFAAGLGCSLREAQTGEKEEAETVSAMALLSVGAEQAQAVATQEHSP R TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001284248
ORF Size	3363 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001284248.1, NP_001271177.1
RefSeq Size	4332 bp
RefSeq ORF	3366 bp
Locus ID	15184
MW	122.4 kDa

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