Tlr7 (NM_001290755) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231523 representing NM_001290755 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGTTTTCGATGTGGACACGGAAGAGACAAATTTTGATCTTTTTAAATATGCTCTTAGTTTCTAGAG TCTTTGGGTTTCGATGGTTTCCTAAAACTCTACCTTGTGAAGTTAAAGTAAATATCCCAGAGGCCCATGT GATCGTGGACTGCACAGACAAGCATTTGACAGAAATCCCTGAGGGCATTCCCACTAACACCACCAATCTT ACCCTTACCATCAACCACATACCAAGCATCTCTCCAGATTCCTTCCGTAGGCTGAACCATCTGGAAGAAA TCGATTTAAGATGCAATTGTGTACCTGTTCTACTGGGGTCCAAAGCCAATGTGTGTACCAAGAGGCTGCA GATTAGACCTGGAAGCTTTAGTGGACTCTCTGACTTAAAAGCCCTTTACCTGGATGGAAACCAACTTCTG GAGATACCACAGGATCTGCCATCCAGCTTACATCTTCTGAGCCTTGAGGCTAACAACATCTTCTCCATCA CGAAGGAGAATCTAACAGAACTGGTCAACATTGAAACACTCTACCTGGGTCAAAACTGTTATTATCGAAA TCCTTGCAATGTTTCCTATTCTATTGAAAAAGATGCTTTCCTAGTTATGAGAAATTTGAAGGTTCTCTCA CTAAAAGATAACAATGTCACAGCTGTCCCCACCACTTTGCCACCTAATTTACTAGAGCTCTATCTTTATA ACAATATCATTAAGAAAATCCAAGAAAATGATTTTAATAACCTCAATGAGTTGCAAGTTCTTGACCTAAG TGGAAATTGCCCTCGATGTTATAATGTCCCATATCCGTGTACACCGTGTGAAAATAATTCCCCCTTACAG ATCCATGACAATGCTTTCAATTCATTGACAGAATTAAAAGTTTTACGTTTACACAGTAATTCTCTTCAGC ATGTGCCCCCAACATGGTTTAAAAACATGAGAAACCTCCAGGAACTAGACCTCTCCCAAAACTACTTGGC CAGAGAAATTGAGGAGGCCAAATTTTTGCATTTTCTTCCCAACCTTGTTGAGTTGGATTTTTCTTTCAAT TATGAGCTGCAGGTCTACCATGCATCTATAACTTTACCACATTCACTCTCTTCATTGGAAAACTTGAAAA TTCTGCGTGTCAAGGGGTATGTCTTTAAAGAGCTGAAAAACTCCAGTCTTTCTGTATTGCACAAGCTTCC CAGGCTGGAAGTTCTTGACCTTGGCACTAACTTCATAAAAATTGCTGACCTCAACATATTCAAACATTTT GAAAACCTCAAACTCATAGACCTTTCAGTGAATAAGATATCTCCTTCAGAAGAGTCAAGAGAAGTTGGCT TTTGTCCTAATGCTCAAACTTCTGTAGACCGTCATGGGCCCCAGGTCCTTGAGGCCTTACACTATTTCCG ATACGATGAATATGCACGGAGCTGCAGGTTCAAAAACAAAGAGCCACCTTCTTTCTTGCCTTTGAATGCA GACTGCCACATATATGGGCAGACCTTAGACTTAAGTAGAAATAACATATTTTTTATTAAACCTTCTGATT TTCAGCATCTTTCATTCCTCAAATGCCTCAACTTATCAGGAAACACCATTGGCCAAACTCTTAATGGCAG TGAACTCTGGCCGTTGAGAGAGTTGCGGTACTTAGACTTCTCCAACAACCGGCTTGATTTACTCTACTCA ACAGCCTTTGAAGAGCTCCAGAGTCTTGAAGTTCTGGATCTAAGTAGTAACAGCCACTATTTTCAAGCAG AAGGAATTACTCACATGCTAAACTTTACCAAGAAATTACGGCTTCTGGACAAACTCATGATGAATGATAA TGACATCTCTACTTCGGCCAGCAGGACCATGGAAAGTGACTCTCTTCGAATTCTGGAGTTCAGAGGCAAC CATTTAGATGTTCTATGGAGAGCCGGTGATAACAGATACTTGGACTTCTTCAAGAATTTGTTCAATTTAG AGGTATTAGATATCTCCAGAAATTCCCTGAATTCCTTGCCTCCTGAGGTTTTTGAGGGTATGCCGCCAAA TCTAAAGAATCTCTCCTTGGCCAAAAATGGGCTCAAATCTTTCTTTTGGGACAGACTCCAGTTACTGAAG CATTTGGAAATTTTGGACCTCAGCCATAACCAGCTGACAAAAGTACCTGAGAGATTGGCCAACTGTTCCA AAAGTCTCACAACACTGATTCTTAAGCATAATCAAATCAGGCAATTGACAAAATATTTTCTAGAAGATGC TTTGCAATTGCGCTATCTAGACATCAGTTCAAATAAAATCCAGGTCATTCAGAAGACTAGCTTCCCAGAA AATGTCCTCAACAATCTGGAGATGTTGGTTTTACATCACAATCGCTTTCTTTGCAACTGTGATGCTGTGT GGTTTGTCTGGTGGGTTAACCATACAGATGTTACTATTCCATACCTGGCCACTGATGTGACTTGTGTAGG TCCAGGAGCACACAAAGGTCAAAGTGTCATATCCCTTGATCTGTATACGTGTGAGTTAGATCTCACAAAC CTGATTCTGTTCTCAGTTTCCATATCATCAGTCCTCTTTCTTATGGTAGTTATGACAACAAGTCACCTCT TTTTCTGGGATATGTGGTACATTTATTATTTTTGGAAAGCAAAGATAAAGGGGTATCAGCATCTGCAATC CATGGAGTCTTGTTATGATGCTTTTATTGTGTATGACACTAAAAACTCAGCTGTGACAGAATGGGTTTTG CAGGAGCTGGTGGCAAAATTGGAAGATCCAAGAGAAAAACACTTCAATTTGTGTCTAGAAGAAAGAGACT GGCTACCAGGACAGCCAGTTCTAGAAAACCTTTCCCAGAGCATACAGCTCAGCAAAAAGACAGTGTTTGT GATGACACAGAAATATGCTAAGACTGAGAGTTTTAAGATGGCATTTTATTTGTCTCATCAGAGGCTCCTG GATGAAAAAGTGGATGTGATTATCTTGATATTCTTGGAAAAGCCTCTTCAGAAGTCTAAGTTTCTTCAGC TCAGGAAGAGACTCTGCAGGAGCTCTGTCCTTGAGTGGCCTGCAAATCCACAGGCTCACCCATACTTCTG GCAGTGCCTGAAAAATGCCCTGACCACAGACAATCATGTGGCTTATAGTCAAATGTTCAAGGAAACAGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231523 representing NM_001290755 Red=Cloning site Green=Tags(s) MVFSMWTRKRQILIFLNMLLVSRVFGFRWFPKTLPCEVKVNIPEAHVIVDCTDKHLTEIPEGIPTNTTNL TLTINHIPSISPDSFRRLNHLEEIDLRCNCVPVLLGSKANVCTKRLQIRPGSFSGLSDLKALYLDGNQLL EIPQDLPSSLHLLSLEANNIFSITKENLTELVNIETLYLGQNCYYRNPCNVSYSIEKDAFLVMRNLKVLS LKDNNVTAVPTTLPPNLLELYLYNNIIKKIQENDFNNLNELQVLDLSGNCPRCYNVPYPCTPCENNSPLQ IHDNAFNSLTELKVLRLHSNSLQHVPPTWFKNMRNLQELDLSQNYLAREIEEAKFLHFLPNLVELDFSFN YELQVYHASITLPHSLSSLENLKILRVKGYVFKELKNSSLSVLHKLPRLEVLDLGTNFIKIADLNIFKHF ENLKLIDLSVNKISPSEESREVGFCPNAQTSVDRHGPQVLEALHYFRYDEYARSCRFKNKEPPSFLPLNA DCHIYGQTLDLSRNNIFFIKPSDFQHLSFLKCLNLSGNTIGQTLNGSELWPLRELRYLDFSNNRLDLLYS TAFEELQSLEVLDLSSNSHYFQAEGITHMLNFTKKLRLLDKLMMNDNDISTSASRTMESDSLRILEFRGN HLDVLWRAGDNRYLDFFKNLFNLEVLDISRNSLNSLPPEVFEGMPPNLKNLSLAKNGLKSFFWDRLQLLK HLEILDLSHNQLTKVPERLANCSKSLTTLILKHNQIRQLTKYFLEDALQLRYLDISSNKIQVIQKTSFPE NVLNNLEMLVLHHNRFLCNCDAVWFVWWVNHTDVTIPYLATDVTCVGPGAHKGQSVISLDLYTCELDLTN LILFSVSISSVLFLMVVMTTSHLFFWDMWYIYYFWKAKIKGYQHLQSMESCYDAFIVYDTKNSAVTEWVL QELVAKLEDPREKHFNLCLEERDWLPGQPVLENLSQSIQLSKKTVFVMTQKYAKTESFKMAFYLSHQRLL DEKVDVIILIFLEKPLQKSKFLQLRKRLCRSSVLEWPANPQAHPYFWQCLKNALTTDNHVAYSQMFKETV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001290755
ORF Size	3150 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001290755.1, NP_001277684.1
RefSeq Size	3943 bp
RefSeq ORF	3153 bp
Locus ID	170743
UniProt ID	P58681
MW	121.8 kDa
Gene Summary	Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR7 is a nucleotide-sensing TLR which is activated by single-stranded RNA. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.[UniProtKB/Swiss-Prot Function]

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