Pear1 (NM_001032413) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	3110045G13Rik; Jedi-1; Megf12
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231505 representing NM_001032413 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCACTTTGTCCCCTCCTCCTCCTGGCCCTAGGCCTGCGTCTGACTGGGACACTCAACTCCAATGATC CCAATGTCTGTACCTTTTGGGAAAGCTTCACCACGACCACTAAGGAGTCCCACCTGCGCCCCTTCAGCCT GCTCCCAGCTGAGTCCTGCCACAGGCCCTGGGAGGACCCCCACACCTGTGCCCAGCCTACGGTTGTCTAC CGGACTGTGTACCGTCAGGTGGTGAAGATGGACTCCCGCCCACGCCTGCAGTGCTGTAGGGGTTACTACG AGAGCAGAGGGGCCTGTGTCCCACTCTGTGCCCAGGAGTGTGTCCATGGTCGCTGTGTGGCTCCGAATCA GTGCCAGTGTGCACCAGGCTGGCGGGGTGGCGACTGCTCCAGCGAGTGTGCCCCGGGAATGTGGGGACCA CAGTGTGACAAGTTCTGCCACTGTGGCAACAACAGTTCCTGTGATCCCAAGAGTGGGACGTGCTTTTGCC CCTCTGGCCTGCAGCCCCCCAACTGCCTTCAGCCCTGCCCTGCCGGCCACTATGGTCCTGCCTGCCAGTT TGATTGCCAGTGCTATGGGGCATCCTGTGACCCCCAGGATGGAGCCTGTTTCTGCCCTCCAGGGAGAGCA GGACCCAGCTGTAATGTGCCCTGTTCACAGGGCACTGATGGCTTCTTCTGCCCCAGAACCTATCCTTGCC AAAATGGAGGTGTTCCTCAGGGCTCCCAAGGCTCCTGCAGCTGCCCACCGGGCTGGATGGGTGTCATTTG TTCCCTGCCATGCCCAGAGGGTTTCCATGGACCCAACTGTACTCAGGAATGTCGCTGCCACAACGGTGGC CTCTGTGACAGGTTTACTGGGCAGTGCCACTGTGCTCCTGGCTATATCGGGGATCGGTGCCAAGAAGAGT GCCCCGTGGGCCGCTTCGGTCAAGACTGTGCTGAGACCTGTGACTGTGCTCCTGGCGCCCGTTGCTTTCC TGCTAATGGCGCGTGTCTGTGTGAACATGGCTTCACAGGCGACCGCTGCACTGAGCGCCTCTGTCCGGAT GGCCGCTATGGTCTGAGCTGCCAGGAGCCCTGCACCTGCGACCCAGAACACAGTCTCAGCTGCCACCCGA TGCACGGCGAGTGCTCCTGCCAGCCAGGTTGGGCGGGCCTCCACTGCAACGAGAGCTGCCCTCAGGACAC GCATGGCCCGGGCTGCCAGGAGCACTGCCTCTGTCTGCACGGAGGGCTCTGCCTTGCCGACAGCGGCCTC TGCCGGTGCGCGCCGGGATACACGGGACCTCACTGCGCTAACCTATGTCCACCGGACACTTACGGGATCA ACTGTTCCTCCCGCTGCTCCTGTGAAAATGCCATTGCCTGCTCTCCCATCGACGGCACGTGCATCTGCAA GGAAGGTTGGCAGCGTGGTAACTGCTCTGTTCCCTGTCCCCTTGGCACCTGGGGCTTCAATTGCAATGCC AGTTGCCAGTGTGCCCACGACGGAGTCTGCAGCCCCCAAACTGGAGCCTGTACTTGCACCCCTGGGTGGC ATGGTGCTCACTGCCAGCTTCCCTGCCCGAAGGGACAGTTTGGTGAAGGCTGTGCCAGTGTCTGTGACTG TGACCACTCTGATGGCTGTGACCCTGTTCATGGACAGTGCCGATGTCAGGCTGGTTGGATGGGCACACGC TGCCACCTGCCTTGCCCGGAGGGCTTTTGGGGAGCCAACTGCAGTAACACCTGTACCTGCAAGAATGGTG GTACCTGTGTGTCTGAGAATGGCAACTGCGTGTGCGCACCAGGGTTCCGAGGCCCCTCCTGCCAGAGGCC CTGCCCGCCTGGTCGCTATGGCAAACGCTGTGTGCAATGCAAGTGTAACAACAACCATTCTTCCTGCCAC CCATCGGACGGGACCTGCTCCTGCCTGGCGGGCTGGACAGGCCCTGACTGCTCCGAGGCATGTCCCCCAG GCCACTGGGGACTCAAATGCTCCCAACTCTGCCAGTGTCATCATGGTGGGACCTGCCACCCCCAGGATGG GAGCTGTATCTGCACGCCAGGCTGGACTGGACCCAACTGCTTGGAAGGCTGCCCACCAAGAATGTTTGGT GTCAACTGCTCCCAGCTATGTCAGTGTGATCTCGGAGAGATGTGCCACCCAGAGACTGGGGCTTGTGTCT GTCCCCCAGGACACAGTGGTGCAGACTGCAAAATGGGAAGCCAGGAGTCCTTCACCATAATGCCCACCTC TCCCGTGACCCATAACTCACTGGGTGCAGTGATTGGCATTGCAGTACTGGGAACCCTCGTGGTGGCCCTG ATAGCACTGTTCATTGGCTACCGCCAGTGGCAAAAGGGCAAGGAACATGAGCACTTGGCAGTGGCTTACA GCACTGGGCGGCTGGATGGCTCTGATTACGTCATGCCAGATGTCTCTCCGAGCTATAGTCACTACTACTC CAACCCCAGCTACCACACACTGTCTCAGTGTTCTCCTAACCCCCCACCCCCTAACAAGGTCCCAGGCAGT CAGCTCTTTGTCAGCTCCCAGGCCCCTGAGCGGCCAAGCAGAGCCCACGGGCGTGAGAACCATGTCACAC TGCCCGCTGACTGGAAGCACCGCCGGGAGCCCCATGAAAGAGGCGCCAGCCACCTGGACCGAAGCTATAG CTGTAGCTATAGCCACAGGAATGGCCCGGGACCATTCTGTCATAAAGGTCCCATCTCTGAAGAGGGACTA GGGGCAAGCGTTATGTCCCTGAGCAGTGAGAACCCCTATGCTACCATCCGAGACCTGCCCAGCCTGCCTG GGGAACCCCGAGAAAGCGGCTATGTGGAGATGAAAGGACCTCCATCAGTGTCCCCTCCCAGGCAGTCTCT TCATCTCCGGGACAGGCAGCAGCGGCAACTGCAGCCACAGAGGGACAGTGGCACCTATGAGCAGCCCAGC CCCTTGAGCCATAATGAAGAGTCTTTGGGCTCCACGCCCCCGCTTCCTCCAGGCCTGCCTCCTGGTCACT ACGACTCCCCCAAGAACAGCCATATCCCTGGACACTATGACTTGCCTCCAGTACGGCATCCTCCATCCCC TCCATCCCGGCGCCAGGACCGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231505 representing NM_001032413 Red=Cloning site Green=Tags(s) MPLCPLLLLALGLRLTGTLNSNDPNVCTFWESFTTTTKESHLRPFSLLPAESCHRPWEDPHTCAQPTVVY RTVYRQVVKMDSRPRLQCCRGYYESRGACVPLCAQECVHGRCVAPNQCQCAPGWRGGDCSSECAPGMWGP QCDKFCHCGNNSSCDPKSGTCFCPSGLQPPNCLQPCPAGHYGPACQFDCQCYGASCDPQDGACFCPPGRA GPSCNVPCSQGTDGFFCPRTYPCQNGGVPQGSQGSCSCPPGWMGVICSLPCPEGFHGPNCTQECRCHNGG LCDRFTGQCHCAPGYIGDRCQEECPVGRFGQDCAETCDCAPGARCFPANGACLCEHGFTGDRCTERLCPD GRYGLSCQEPCTCDPEHSLSCHPMHGECSCQPGWAGLHCNESCPQDTHGPGCQEHCLCLHGGLCLADSGL CRCAPGYTGPHCANLCPPDTYGINCSSRCSCENAIACSPIDGTCICKEGWQRGNCSVPCPLGTWGFNCNA SCQCAHDGVCSPQTGACTCTPGWHGAHCQLPCPKGQFGEGCASVCDCDHSDGCDPVHGQCRCQAGWMGTR CHLPCPEGFWGANCSNTCTCKNGGTCVSENGNCVCAPGFRGPSCQRPCPPGRYGKRCVQCKCNNNHSSCH PSDGTCSCLAGWTGPDCSEACPPGHWGLKCSQLCQCHHGGTCHPQDGSCICTPGWTGPNCLEGCPPRMFG VNCSQLCQCDLGEMCHPETGACVCPPGHSGADCKMGSQESFTIMPTSPVTHNSLGAVIGIAVLGTLVVAL IALFIGYRQWQKGKEHEHLAVAYSTGRLDGSDYVMPDVSPSYSHYYSNPSYHTLSQCSPNPPPPNKVPGS QLFVSSQAPERPSRAHGRENHVTLPADWKHRREPHERGASHLDRSYSCSYSHRNGPGPFCHKGPISEEGL GASVMSLSSENPYATIRDLPSLPGEPRESGYVEMKGPPSVSPPRQSLHLRDRQQRQLQPQRDSGTYEQPS PLSHNEESLGSTPPLPPGLPPGHYDSPKNSHIPGHYDLPPVRHPPSPPSRRQDR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001032413
ORF Size	3102 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001032413.1, NP_001027585.1
RefSeq Size	4367 bp
RefSeq ORF	3105 bp
Locus ID	73182
UniProt ID	Q8VIK5
MW	110.6 kDa
Gene Summary	When overexpressed, reduces the number of both early and late non-adherent myeloid progenitor cells.[UniProtKB/Swiss-Prot Function]

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