Ano5 (NM_001271879) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	9330162L24; Gdd1; Tmem16e
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231263 representing NM_001271879 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGGAGCAGGAAGGCTTAACAGCCAAAGAAATAGACTATGCTTTCCAACAGAATGAGAACCTGGGCT CCAAAGAGACCAGCTTTCTCATCCCTGAAGACTTACAGTCACCGTTCCAGAGAAGTGAGCACAGCAAAGA CTCGGTCTTCTTCAGAGATGGAATCCGGCAGATCGACTTTGTCCTGTCCTACGTGGAGGATTTGAAGAAA GATGGAGAGCTGAAGGCAGAAAGAAGACGAGAGTTTGAACAAAATCTCAGAAAAACAGGTCTTGACTTGG AAACAGAAGACAAATTGAACTCTGAAGATGGGAAAACCTATTTTGTGAAGATTCACGCCCCATGGGAAGT CCTGGTCACTTACGCTGAAGTGCTGGGAATCAAGATGCCTATTAAGTTGAGTGACATCCCACGGCCAAAG TACCCACCCCTGTCTTACATGCTTGGGGCGGTGAAGCTTCCATCCTCTGTGAAGTATCCTACTCCTGAGT ATTTCACTGCCCAGTTCAGCAGACACCGTCAAGAACTCTTCCTCATTGAAGATGAGGCCACTTTCTTTCC ATCCTCAACAAGGAACAGAATTGTTTACTATATTCTGTCACGATGCCCTTTTGGAGTGGAAGAGGGGAAG AAAAAGATTGGGATCGAGAGACTGCTGAACTCGAACACTTATTTATCTGCCTACCCGCTCCATGATGGCC AGTATTGGAAACCATCAAAAACTACTCGCCCCAATGAAAGGTACAACCTTTGCAAGAACTGGGCTCGATT TTCCTACTTTTACAAGGAGCAACCTTTTCATTTGATTCGGAATTATTTTGGAGAAAAAATCGGTATCTAT TTTGTATTTCTCGGGTATTACACAGAAATGCTGTTGTTTGCGGCTCTGGTCGGCTTAGCCTGCTTCATCT ATGGCTTGTTATCAATGGAAAATAACCGAACTAGCACTGAAATCTGTGACCCTGACATCGGAGGTCAGAT GATCATGTGCCCACTTTGCGATGAAGTGTGTGATTACTGGAGACTGAACACCACGTGTTTGCATTCAAAG TTCTCCCATCTGTTTGATAACGAGTCGACTGTGTTCTTTGCGCTCTTCATGGGGATCTGGGTCACATTGT TTCTGGAGTTTTGGAAGCAGCGACAAGCCAGGCTTGAGTACGAATGGGATCTGGTGGACTTTGAAGAAGA ACAGCAGCAGCTCCAGCTTCGGCCTGAATTTGAAGCTATGTGTAAACACAAGAAGATGAATCCTGTGACA AAGGAAATGGAGCCACACATGCCTCTATGCCATCGCATCCCATGGTACTTTGTGTCAGGGACCACAGTGA CCTTTGGGATGGCTCTCCTCCTCAGCAGCATGGTGTCCATCCTCATATACCGCCTGTCAGTCTTTGCCAC TTTTGCCAGCTTCATGGAAAGCGAAGCCACCCTGCAGAGCGTGAAGAGCTTCTTTACTCCACAGCTGGCT ACGGCTCTCTCTGGATCATGCCTGAACTGCATTGTCATTTTGATACTGAATTTTTTTTATGAGAAGATAT CTGCCTGGATTACGAAAATGGGGAAGTTCGTGGGATATCCTGGGAGTTACACATATATGTTCAACATATG GAGAAGCGAAGAGTGTGGTCCTGCAGGCTGTCTGATAGAGCTGACCACCCAGCTAACCATCATTATGATT GGAAAACAGATTTTTGGAAACATCCATGAAGCCTTTCAACCCTTGATTTTTAATTGGTGGAGACGCCGAA GAGCCCGAACCCACTCTGAGAAACTGTACAGTCGTTGGGAGCAAGATCATGACCTTCAGGTTTATGGACA TCGTGGGCTGTTTTATGAGTACTTGGAAACAGTTATCCAGTTTGGGTTTGCCACGCTGTTTGTGGCCTCT TTTCCTCTGGCACCCCTGTTTGCTCTCATGAACAACATCATGGGAATCCGAGTGGATGCCTGGAAGCTGA CAACTCAGTACAGGAGACCTGTCGCAGCGAAAGCCCACTCCATAGGTGTCTGGCAAGACATTCTTTTTGG AATGGCCATCGTTTCTGTTGCAACTAATGCCTTCATTGTCTCATTTACATCTGACATCATCCCCCGGTTA GTTTACTTCTATGCCTACTCAACGAATTCCACAGAGCCCTTGAGTGGATACGTCAATAACAGCCTGTCAG TGTTCCTGATAGCTGACTTTCCCAACCACACAGTGCCCATGGAGAAAAAAGACTTCGTCACCTGCAGGTA CAGAGATTATAGGTACCCTCCTGATCATGAGGATAAGTATAGCCATAACATGCAGTTCTGGCATGTTCTT GCTGCCAAGATGACCTTCATCATAGTGATGGAACACGTCGTGTTTCTGTTTAAATTTTTATTAGCCTGGC TGATACCAGATGTTCCTAAAGATGTTGTGGAGAAAATCAAACGAGAAAAGTTAATGACTATCAAAATTAT TCATGACTTTGAGCTCAACAAGTTGAAAGAAAACTTGGATGTTGAATATGGTAACATCATGAAGAATGTT TTAGTAGATGAAGACAATTCCCTGAAGGCTAAAACTACAGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231263 representing NM_001271879 Red=Cloning site Green=Tags(s) MVEQEGLTAKEIDYAFQQNENLGSKETSFLIPEDLQSPFQRSEHSKDSVFFRDGIRQIDFVLSYVEDLKK DGELKAERRREFEQNLRKTGLDLETEDKLNSEDGKTYFVKIHAPWEVLVTYAEVLGIKMPIKLSDIPRPK YPPLSYMLGAVKLPSSVKYPTPEYFTAQFSRHRQELFLIEDEATFFPSSTRNRIVYYILSRCPFGVEEGK KKIGIERLLNSNTYLSAYPLHDGQYWKPSKTTRPNERYNLCKNWARFSYFYKEQPFHLIRNYFGEKIGIY FVFLGYYTEMLLFAALVGLACFIYGLLSMENNRTSTEICDPDIGGQMIMCPLCDEVCDYWRLNTTCLHSK FSHLFDNESTVFFALFMGIWVTLFLEFWKQRQARLEYEWDLVDFEEEQQQLQLRPEFEAMCKHKKMNPVT KEMEPHMPLCHRIPWYFVSGTTVTFGMALLLSSMVSILIYRLSVFATFASFMESEATLQSVKSFFTPQLA TALSGSCLNCIVILILNFFYEKISAWITKMGKFVGYPGSYTYMFNIWRSEECGPAGCLIELTTQLTIIMI GKQIFGNIHEAFQPLIFNWWRRRRARTHSEKLYSRWEQDHDLQVYGHRGLFYEYLETVIQFGFATLFVAS FPLAPLFALMNNIMGIRVDAWKLTTQYRRPVAAKAHSIGVWQDILFGMAIVSVATNAFIVSFTSDIIPRL VYFYAYSTNSTEPLSGYVNNSLSVFLIADFPNHTVPMEKKDFVTCRYRDYRYPPDHEDKYSHNMQFWHVL AAKMTFIIVMEHVVFLFKFLLAWLIPDVPKDVVEKIKREKLMTIKIIHDFELNKLKENLDVEYGNIMKNV LVDEDNSLKAKTTV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001271879
ORF Size	2562 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271879.1, NP_001258808.1
RefSeq Size	7633 bp
RefSeq ORF	2565 bp
Locus ID	233246
UniProt ID	Q75UR0
MW	100.4 kDa
Gene Summary	This gene encodes a member of the anoctamin family, which in mammals is comprised of 10 members. Anoctamin proteins are proposed to have eight transmembrane domains with both termini facing the cytoplasm and a C-terminal domain of unknown function. While some members have been characterized as calcium-activated chloride channels, this protein is reported to have little anion conductance activity. Elevated levels of this protein were found in dystrophic mice. In humans, mutations of this gene are associated with with musculoskeletal disorders such as myopathies, muscular dystrophy and gnathodiaphyseal dysplasia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2012]

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