Eps8 (NM_001271587) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AW261790
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231250 representing NM_001271587 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCTCTCAGTCTTGCCTTGACAGTTGCTTGGACGAGTTTTACCTCAGCAGTGCAGGAAGAGGCGTGA GAGCCGGCATGAATGGTCATATGTCTAACCGCTCCAGTGGGTATGGAGTCTACCCTTCTCAACTGAATGG TTACGGATCTTCACCACCCTATTCCCAGATGGACAGAGAACACAGCTCAAGAACAAGTGCAAAGGCCCTT TATGAACAAAGGAAGAACTATGCCCGAGACAGTGTCAGCAGTGTGTCGGACGTGTCCCAGTACCGCGTGG AACACTTGACCACCTTCGTGCTGGATCGGAAAGATGCAATGATCACTGTCGAGGACGGAATAAGAAAGCT GAAGTTGCTGGATGCCAAGGGCAAAGTGTGGACTCAAGATATGATTCTCCAAGTGGATGACCGAGCTGTG AGCCTGATTGACTTAGAGTCAAAGAATGAATTGGAGAATTTTCCTCTAAACACAATCTCGCATTGTCAAG CAGTGGTACATGCATGCAGCTATGACTCCATTCTCGCCTTGGTATGCAAAGAGCCAACGCAGAGCAAGCC AGACCTTCACCTTTTCCAGTGTGATGAGGTTAAGGCAAACCTAATTAGTGAAGATATCGAAAGTGCAATC AGTGACAGTAAAGGTGGGAAACAGAAGAGGCGGCCGGAGGCCCTGAGGATGATTGCCAAAGCAGATCCTG GCATCCCTCCTCCTCCCAGAGCTCCTGCCCCTGTGCCACCAGGGACTGTCACACAGGTGGACGTTAGGAG TCGCGTAGCAGCCTGGTCTGCCTGGGCAGCTGACCAGGGCGACTTCGAGAAGCCCCGGCAGTACCACGAG CAAGAAGAGACGCCCGAGATGATGGCAGCCCGGATCGACAGGGATGTGCAAATCTTAAACCATATTTTGG ATGACATTGAATTTTTTATCACCAAACTCCAAAAAGCCGCCGAAGCGTTTTCTGAGCTTTCTAAAAGGAA GAAAAGTAAGAAAAGTAAAAGGAAAGGACCTGGAGAGGGCGTTTTAACACTGAGGGCAAAACCGCCACCT CCTGATGAATTTGTTGACTGTTTCCAGAAGTTTAAACATGGATTCAACCTTCTGGCCAAGTTGAAGTCCC ATATCCAGAACCCGAGTGCTTCAGATCTGGTTCATTTTTTGTTTACTCCACTAAATATGGTGGTCCAGGC AACAGGTGGCCCCGAACTGGCCAGTTCGGTACTCAGCCCACTGTTGACAAAAGACACAGTTGATTTCTTA AACTACACAGCCACTGCGGAGGAACGGAAGCTGTGGATGTCACTGGGAGATAGTTGGGTGAAAGTGAGAG CAGAGTGGCCGAAAGAACAGTTCATCCCACCTTACGTCCCGAGGTTCCGCAACGGCTGGGAGCCCCCGAT GCTGAACTTCATGGGCGCGCCCACAGAGCAAGACATGTATCAACTGGCCGAGTCCGTGGCCAACGCAGAA CACCAGCGCAAACAGGACAGCAAGAGGCTGTCCACAGAGCATTCCAATGTGTCCGACTATCCTCCAGCCG ACGGATATGCGTACAGTAGCAGCATGTACCACAGAGGACCACATGCAGACCACGGGGAGGCTGCCATGCC TTTCAAGTCAACTCCTAATCACCAAGTAGATAGGAATTATGACGCAGTCAAAACACAACCCAAGAAATAC GCCAAATCCAAGTACGACTTTGTGGCGAGGAACAGCAGCGAGCTCTCGGTTATGAAAGATGATGTCTTAG AGATACTCGACGATCGAAGGCAGTGGTGGAAAGTCCGGAATGCCAGTGGAGACTCTGGGTTTGTGCCAAA TAACATTCTGGATATCATGAGAACTCCGGAATCTGGAGTGGGGCGCGCTGACCCCCCATACACACATACC ATACAGAAACAAAGGACGGAATACGGCCTGAGATCAGCTGACACTCCTTCTGCCCCATCACCCCCTCCAA CGCCAGCACCCGTTCCGGTCCCCCTTCCACCTTCTGTACCAGCACCCGTTTCTGTGCCCAAGGTCCCAGC CAATGTCACCCGCCAGAACAGCAGCTCCAGTGACAGTGGGGGCAGCATTGTGCGGGACAGCCAGAGATAC AAACAACTCCCAGTGGACCGAAGGAAGTCCCAGATGGAAGAGGTTCAGGATGAGCTCTTCCAGAGGCTGA CCATCGGGCGCAGTGCTGCACAGAGGAAGTTCCACGTGCCACGGCAGAACGTTCCAGTGATCAATATCAC TTATGACTCCTCACCGGAAGAAGTAAAGACTTGGCTGCAGTCAAAGGGATTCAACCCCGTGACTGTCAAT AGCCTCGGGGTGTTGAACGGAGCACAACTCTTTTCTCTCAACAAAGACGAACTGAGGTCTGTCTGCCCGG AAGGTGCCAGAGTCTTTAACCAAATCACTGTTCAGAAAGCTGCTTTGGAGGACAGTAATGGAAGCTCCGA GTTACAAGAGATCATGCGGAGACGGCAGGAGAAGATCAGCGCCGCTGCGAGCGACTCGGGAGTGGAGTCT TTCGATGAAGGGAGCAGCCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231250 representing NM_001271587 Red=Cloning site Green=Tags(s) MLSQSCLDSCLDEFYLSSAGRGVRAGMNGHMSNRSSGYGVYPSQLNGYGSSPPYSQMDREHSSRTSAKAL YEQRKNYARDSVSSVSDVSQYRVEHLTTFVLDRKDAMITVEDGIRKLKLLDAKGKVWTQDMILQVDDRAV SLIDLESKNELENFPLNTISHCQAVVHACSYDSILALVCKEPTQSKPDLHLFQCDEVKANLISEDIESAI SDSKGGKQKRRPEALRMIAKADPGIPPPPRAPAPVPPGTVTQVDVRSRVAAWSAWAADQGDFEKPRQYHE QEETPEMMAARIDRDVQILNHILDDIEFFITKLQKAAEAFSELSKRKKSKKSKRKGPGEGVLTLRAKPPP PDEFVDCFQKFKHGFNLLAKLKSHIQNPSASDLVHFLFTPLNMVVQATGGPELASSVLSPLLTKDTVDFL NYTATAEERKLWMSLGDSWVKVRAEWPKEQFIPPYVPRFRNGWEPPMLNFMGAPTEQDMYQLAESVANAE HQRKQDSKRLSTEHSNVSDYPPADGYAYSSSMYHRGPHADHGEAAMPFKSTPNHQVDRNYDAVKTQPKKY AKSKYDFVARNSSELSVMKDDVLEILDDRRQWWKVRNASGDSGFVPNNILDIMRTPESGVGRADPPYTHT IQKQRTEYGLRSADTPSAPSPPPTPAPVPVPLPPSVPAPVSVPKVPANVTRQNSSSSDSGGSIVRDSQRY KQLPVDRRKSQMEEVQDELFQRLTIGRSAAQRKFHVPRQNVPVINITYDSSPEEVKTWLQSKGFNPVTVN SLGVLNGAQLFSLNKDELRSVCPEGARVFNQITVQKAALEDSNGSSELQEIMRRRQEKISAAASDSGVES FDEGSSH TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001271587
ORF Size	2541 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271587.1, NP_001258516.1
RefSeq Size	4561 bp
RefSeq ORF	2544 bp
Locus ID	13860
UniProt ID	Q08509
MW	94.9 kDa
Gene Summary	Signaling adapter that controls various cellular protrusions by regulating actin cytoskeleton dynamics and architecture. Depending on its association with other signal transducers, can regulate different processes. Together with SOS1 and ABI1, forms a trimeric complex that participates in transduction of signals from Ras to Rac by activating the Rac-specific guanine nucleotide exchange factor (GEF) activity. Acts as a direct regulator of actin dynamics by binding actin filaments and has both barbed-end actin filament capping and actin bundling activities depending on the context. Displays barbed-end actin capping activity when associated with ABI1, thereby regulating actin-based motility process: capping activity is auto-inhibited and inhibition is relieved upon ABI1 interaction. Also shows actin bundling activity when associated with BAIAP2, enhancing BAIAP2-dependent membrane extensions and promoting filopodial protrusions. Involved in the regulation of processes such as axonal filopodia growth, stereocilia length, dendritic cell migration and cancer cell migration and invasion. Acts as a regulator of axonal filopodia formation in neurons: in the absence of neurotrophic factors, negatively regulates axonal filopodia formation via actin-capping activity. In contrast, it is phosphorylated in the presence of BDNF leading to inhibition of its actin-capping activity and stimulation of filopodia formation. Component of a complex with WHRN and MYO15A that localizes at stereocilia tips and is required for elongation of the stereocilia actin core. Indirectly involved in cell cycle progression; its degradation following ubiquitination being required during G2 phase to promote cell shape changes.[UniProtKB/Swiss-Prot Function]

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