Pi4kb (NM_001293715) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ESTM41; PI4K-beta; PI4Kbeta; Pik4cb
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231200 representing NM_001293715 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAGACATGGTAGTGGAGCCTGCCACCCTGAAGCCAACTTCTGAGCCTACTCCTAGCCCATCAGGGA ATAATGGGGGCTCCCTACTAAGCGTCATCACGGAGGGGGTCGGGGAACTGTCAGTGATTGACCCTGAGGT GGCCCAGAAGGCCTGCCAGGAGGTACTGGAGAAAGTCAAGCTTTTGCATGGAGGTGTAGCCATCTCTAGC AAAGGCACCCCGCTGGAGTTGGTTAATGGGGATGGTGTGGACAATGAAATCCGTTGCCTAGATGATCCAC CTGCCCAGATCAGGGAGGAAGAAGATGAGATGGGGGCTGGTGTGGCCTCTGGCACAGCCAAAGGAGCAAG ACGACGACGACAGAACAACTCAGCCAAACAGTCTTGGCTCCTGAGGCTGTTTGAGTCAAAACTATTTGAC ATCTCTATGGCTATTTCATACTTGTATAACTCCAAGGAGCCTGGAGTGCAAGCCTACATTGGCAACCGGC TCTTCTACTTTCGCAATGAGGATGTGGACTTCTATTTGCCCCAGTTGCTTAACATGTATATCCACATGGA TGAGGATGTGGGCGATGCCATTAAGCCCTACATAGTCCACCGCTGTCGCCAGAGCATCAACTTTTCCCTC CAGTGTGCCCTGTTGCTTGGGGCCTACTCTTCAGACATGCACATTTCCACTCAGCGACACTCGCGAGGGA CCAAGTTACGGAAGCTAATCCTCTCAGATGAGCTGAAGCCAGCTCACCGAAAGAGGGAGCTGCCGACATT AAGCCCAGCCCCTGACACAGGGCTGTCTCCCTCTAAAAGGACTCACCAGCGTTCTAAGTCAGATGCTACG GCCAGCATAAGTCTCAGCAGCAACCTGAAACGAACAGCCAGCAACCCTAAAGTGGAGAATGAGGATGAGG AGCTCTCCTCCAGCACCGAGAGTATTGATAATTCATTCAGTTCCCCTGTCCGGCTGGCCCCTGAGCGAGA ATTCATCAAGTCTTTGATGGCAATCGGCAAACGGCTGGCCACGCTCCCCACTAAAGAGCAAAAAACACAA AGGCTGATCTCAGAGCTTTCCCTGCTCAACCATAAGCTCCCTGCCCGAGTCTGGCTGCCCACGGCTGGCT TTGACCACCACGTGGTCCGTGTGCCCCACACACAAGCTGTTGTTCTCAATTCCAAGGATAAGGCTCCCTA CCTGATCTACGTGGAAGTTCTCGAATGTGAAAACTTTGACACAACTAGTGTTCCTGCCCGGATTCCTGAA AACCGAATTCGGAGTACACGGTCTGTAGAGAACCTGCCTGAATGTGGTATCACTCATGAGCAGCGAGCTG GCAGCTTCAGCACTGTGCCCAATTATGACAATGATGATGAAGCCTGGTCAGTGGATGATATAGGCGAGCT GCAAGTGGAGCTCCCTGAAGTGCACACCAACAGCTGTGACAACATCTCCCAGTTCTCGGTGGACAGCATC ACCAGCCAGGAGAGCAAGGAGCCTGTGTTCATTGCAGCAGGGGACATCCGACGGCGCCTTTCAGAACAGC TGGCTCATACTCCTACAGCCTTCAAACGAGACCCTGAAGACCCTTCTGCAGTTGCCCTCAAAGAGCCCTG GCAGGAGAAAGTGCGGAGGATCAGAGAAGGTTCCCCGTATGGCCATCTTCCCAATTGGCGACTCCTTTCA GTCATTGTCAAGTGTGGAGATGACCTTCGCCAGGAGCTGCTGGCTTTCCAGGTGTTGAAACAACTGCAGT CCATTTGGGAACAGGAGCGAGTGCCTCTTTGGATCAAGCCATATAAGATTCTTGTGATTTCAGCTGACAG TGGCATGATTGAACCAGTAGTCAACGCTGTGTCCATCCACCAGGTGAAGAAACAGTCACAGCTCTCCTTG CTCGATTACTTCCTACAGGAACATGGCAGTTATACCACTGAGGCATTCCTCAGTGCCCAGCGCAATTTTG TGCAAAGCTGTGCTGGCTACTGCTTGGTCTGCTACCTATTGCAAGTCAAGGACAGGCACAACGGGAACAT CCTTCTGGACGCAGAAGGTCACATCATCCACATCGACTTTGGCTTCATCCTTTCCAGCTCACCCCGAAAC CTGGGCTTCGAGACATCAGCCTTTAAGCTGACCACAGAATTTGTGGATGTAATGGGTGGCCTGAACGGTG ATATGTTCAACTACTACAAGATGCTCATGCTGCAAGGGCTGATTGCTGCTCGGAAGCACATGGACAAGGT GGTACAGATTGTGGAGATCATGCAGCAAGGTTCTCAGCTTCCTTGCTTCCATGGCTCCAGCACCATTCGC AACCTCAAAGAGAGGTTCCACATGAGCATGACTGAGGAGCAGCTGCAGCTGCTGGTGGAGCAGATGGTGG ACGGCAGCATGAGGTCCATCACCACCAAACTCTACGATGGCTTCCAGTACCTCACCAATGGCATCATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231200 representing NM_001293715 Red=Cloning site Green=Tags(s) MGDMVVEPATLKPTSEPTPSPSGNNGGSLLSVITEGVGELSVIDPEVAQKACQEVLEKVKLLHGGVAISS KGTPLELVNGDGVDNEIRCLDDPPAQIREEEDEMGAGVASGTAKGARRRRQNNSAKQSWLLRLFESKLFD ISMAISYLYNSKEPGVQAYIGNRLFYFRNEDVDFYLPQLLNMYIHMDEDVGDAIKPYIVHRCRQSINFSL QCALLLGAYSSDMHISTQRHSRGTKLRKLILSDELKPAHRKRELPTLSPAPDTGLSPSKRTHQRSKSDAT ASISLSSNLKRTASNPKVENEDEELSSSTESIDNSFSSPVRLAPEREFIKSLMAIGKRLATLPTKEQKTQ RLISELSLLNHKLPARVWLPTAGFDHHVVRVPHTQAVVLNSKDKAPYLIYVEVLECENFDTTSVPARIPE NRIRSTRSVENLPECGITHEQRAGSFSTVPNYDNDDEAWSVDDIGELQVELPEVHTNSCDNISQFSVDSI TSQESKEPVFIAAGDIRRRLSEQLAHTPTAFKRDPEDPSAVALKEPWQEKVRRIREGSPYGHLPNWRLLS VIVKCGDDLRQELLAFQVLKQLQSIWEQERVPLWIKPYKILVISADSGMIEPVVNAVSIHQVKKQSQLSL LDYFLQEHGSYTTEAFLSAQRNFVQSCAGYCLVCYLLQVKDRHNGNILLDAEGHIIHIDFGFILSSSPRN LGFETSAFKLTTEFVDVMGGLNGDMFNYYKMLMLQGLIAARKHMDKVVQIVEIMQQGSQLPCFHGSSTIR NLKERFHMSMTEEQLQLLVEQMVDGSMRSITTKLYDGFQYLTNGIM TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001293715
ORF Size	2448 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001293715.1, NP_001280644.1
RefSeq Size	3831 bp
RefSeq ORF	2451 bp
Locus ID	107650
UniProt ID	Q8BKC8
MW	92 kDa
Gene Summary	Phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate (PIP). May regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation (By similarity). Involved in Golgi-to-plasma membrane trafficking (By similarity).[UniProtKB/Swiss-Prot Function]

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