Clcn5 (NM_001243762) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	5430408K11Rik; ClC-5; Clc4-1; Clc5; Clcn4-1; D930009B12Rik; DXImx42e; Sfc13; T25545
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231199 representing NM_001243762 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCATGTGGCAGGGAGCCATGGATAACAGAGGCTTTCATCAGGGGAGTTTTAGTAGCTTCCAGAGCA GCTCTAGTGATGAAGACTTGATGGACATCCCAGGGACTGCTATGGACTTCTCTATGAGAGATGACGTGCC GCCTTTAGATCGAGAAATAGAAGGGAACAAGTCATACAATGGTGGTGGAATAGGCTCTTCAAATAGAGTC ATGGACTTCTTGGAGGAGCCAATCCCTGGTGTAGGGACCTATGATGATTTCAACACAATTGATTGGGTGA GAGAGAAATCTCGAGACCGAGATAGGCACCGAGAGATTACCAATAAAAGCAAAGAGTCAACATGGGCCTT AATTCACAGTGTGAGCGATGCTTTCTCTGGTTGGTTGTTAATGCTCCTTATTGGCCTTTTATCAGGTTCC TTAGCTGGCCTGATAGACATCTCTGCTCACTGGATGACAGACTTAAAAGAAGGGATATGTACAGGGGGGT TCTGGTTTAACCATGAGCACTGTTGCTGGAATTCTGAGCATGTTACCTTTGAACACAGAGACAAATGTCC AGAGTGGAATAGCTGGGCCCAGCTCATCATCAATACAGATCAGGGAGCCTTTGCCTACATAGTCAATTAC TTCATGTACGTCCTCTGGGCTCTTCTGTTTGCTTTCCTTGCTGTATCTCTTGTCAAGGCATTTGCACCTT ATGCCTGTGGTTCTGGAATCCCTGAGATCAAAACCATCCTGAGTGGTTTCATTATTAGAGGCTATCTGGG TAAGTGGACCCTTGTCATCAAAACTATCACTTTGGTGTTGGCGGTGTCATCTGGCTTGAGCCTGGGCAAG GAGGGCCCCCTGGTGCACGTGGCTTGCTGCTGTGGGAACATCTTGTGCCACTGCTTCAACAAATACCGGA AGAATGAAGCCAAGCGGAGAGAGGTCTTATCAGCTGCAGCGGCTGCCGGTGTATCTGTAGCCTTTGGGGC CCCTATTGGTGGAGTATTATTCAGCCTGGAAGAGGTCAGCTACTACTTTCCCCTCAAAACACTATGGCGT TCTTTCTTCGCTGCTCTGGTGGCAGCGTTCACACTCCGCTCCATCAATCCATTCGGAAACAGCCGCTTAG TTCTGTTTTATGTGGAGTTCCACACCCCGTGGCATCTCTTTGAGCTCGTGCCATTCATTGTACTAGGCAT ATTCGGTGGCCTCTGGGGAGCTCTGTTTATCCGCACAAACATTGCCTGGTGTCGGAAGCGTAAAACCACA CAGTTGGGCAAGTATCCTGTCGTCGAGGTACTCATTGTGACGGCCATCACTGCCATCTTGGCTTTCCCCA ATGAATATACCCGGATGAGCACAAGTGAGCTCATTTCTGAGCTGTTCAATGACTGCGGCCTCCTGGACTC CTCCAAGCTCTGTGATTATGAGAACCACTTCAACACAAGCAAAGGCGGTGAGCTGCCTGACAGACCTGCT GGCGTGGGCATCTACAGTGCCATGTGGCAGCTGGCTCTGACACTCATACTGAAAATAGTCATTACTATAT TCACCTTTGGCATGAAGATTCCTTCTGGTCTCTTTATCCCCAGCATGGCTGTCGGTGCTATAGCAGGCCG TCTTTTAGGAGTCGGAATGGAGCAACTGGCTTATTACCACCACGACTGGGGCATCTTCAATAGCTGGTGT AGTCAGGGAGCTGATTGTATCACCCCTGGCCTTTATGCCATGGTTGGGGCTGCTGCCTGCTTAGGTGGGG TGACTCGGATGACTGTTTCTCTTGTTGTCATAATGTTTGAACTGACTGGTGGCCTGGAATATATTGTGCC TCTGATGGCTGCAGCTATGACAAGCAAGTGGGTGGCCGATGCTCTTGGGCGAGAGGGCATTTATGATGCC CACATCCGTCTCAATGGATACCCCTTTCTTGAAGCCAAAGAAGAATTTGCTCATAAGACTCTGGCAATGG ATGTGATGAAGCCCCGGAGAAATGACCCCTTGCTGACTGTCCTTACTCAGGACAGTATGACTGTGGAGGA TGTCGAGACCATAATCAGCGAAACCACGTACAGTGGCTTTCCGGTGGTAGTGTCCCGAGAGTCGCAAAGA CTTGTAGGCTTTGTTCTTCGAAGAGATCTCATTATTTCAATTGAAAATGCTCGGAAGAAACAGGACGGGG TTGTGAGCACTTCCATCATTTATTTCACGGAGCATTCGCCTCCAATGCCTCCATACACCCCACCTACCCT GAAGCTTCGGAACATCCTGGACCTCAGCCCCTTCACTGTGACAGACCTTACGCCAATGGAGATCGTGGTG GACATCTTCCGCAAGCTGGGCCTGCGCCAGTGCCTGGTTACACACAACGGGCGCTTACTTGGAATCATCA CCAAAAAGGATGTGTTAAAGCATATAGCACAGATGGCAAACCAAGACCCTGATTCCATTCTCTTCAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231199 representing NM_001243762 Red=Cloning site Green=Tags(s) MAMWQGAMDNRGFHQGSFSSFQSSSSDEDLMDIPGTAMDFSMRDDVPPLDREIEGNKSYNGGGIGSSNRV MDFLEEPIPGVGTYDDFNTIDWVREKSRDRDRHREITNKSKESTWALIHSVSDAFSGWLLMLLIGLLSGS LAGLIDISAHWMTDLKEGICTGGFWFNHEHCCWNSEHVTFEHRDKCPEWNSWAQLIINTDQGAFAYIVNY FMYVLWALLFAFLAVSLVKAFAPYACGSGIPEIKTILSGFIIRGYLGKWTLVIKTITLVLAVSSGLSLGK EGPLVHVACCCGNILCHCFNKYRKNEAKRREVLSAAAAAGVSVAFGAPIGGVLFSLEEVSYYFPLKTLWR SFFAALVAAFTLRSINPFGNSRLVLFYVEFHTPWHLFELVPFIVLGIFGGLWGALFIRTNIAWCRKRKTT QLGKYPVVEVLIVTAITAILAFPNEYTRMSTSELISELFNDCGLLDSSKLCDYENHFNTSKGGELPDRPA GVGIYSAMWQLALTLILKIVITIFTFGMKIPSGLFIPSMAVGAIAGRLLGVGMEQLAYYHHDWGIFNSWC SQGADCITPGLYAMVGAAACLGGVTRMTVSLVVIMFELTGGLEYIVPLMAAAMTSKWVADALGREGIYDA HIRLNGYPFLEAKEEFAHKTLAMDVMKPRRNDPLLTVLTQDSMTVEDVETIISETTYSGFPVVVSRESQR LVGFVLRRDLIISIENARKKQDGVVSTSIIYFTEHSPPMPPYTPPTLKLRNILDLSPFTVTDLTPMEIVV DIFRKLGLRQCLVTHNGRLLGIITKKDVLKHIAQMANQDPDSILFN TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001243762
ORF Size	2448 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001243762.1, NP_001230691.1
RefSeq Size	4170 bp
RefSeq ORF	2451 bp
Locus ID	12728
UniProt ID	Q9WVD4
MW	91.2 kDa
Gene Summary	Proton-coupled chloride transporter. Functions as antiport system and exchanges chloride ions against protons. Important for normal acidification of the endosome lumen. May play an important role in renal tubular function (By similarity).[UniProtKB/Swiss-Prot Function]

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