Clcn5 (NM_001243762) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	5430408K11Rik; ClC-5; Clc4-1; Clc5; Clcn4-1; D930009B12Rik; DXImx42e; Sfc13; T25545
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228988 representing NM_001243762 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCATGTGGCAGGGAGCCATGGATAACAGAGGCTTTCATCAGGGGAGTTTTAGTAGCTTCCAGAGCA GCTCTAGTGATGAAGACTTGATGGACATCCCAGGGACTGCTATGGACTTCTCTATGAGAGATGACGTGCC GCCTTTAGATCGAGAAATAGAAGGGAACAAGTCATACAATGGTGGTGGAATAGGCTCTTCAAATAGAGTC ATGGACTTCTTGGAGGAGCCAATCCCTGGTGTAGGGACCTATGATGATTTCAACACAATTGATTGGGTGA GAGAGAAATCTCGAGACCGAGATAGGCACCGAGAGATTACCAATAAAAGCAAAGAGTCAACATGGGCCTT AATTCACAGTGTGAGCGATGCTTTCTCTGGTTGGTTGTTAATGCTCCTTATTGGCCTTTTATCAGGTTCC TTAGCTGGCCTGATAGACATCTCTGCTCACTGGATGACAGACTTAAAAGAAGGGATATGTACAGGGGGGT TCTGGTTTAACCATGAGCACTGTTGCTGGAATTCTGAGCATGTTACCTTTGAACACAGAGACAAATGTCC AGAGTGGAATAGCTGGGCCCAGCTCATCATCAATACAGATCAGGGAGCCTTTGCCTACATAGTCAATTAC TTCATGTACGTCCTCTGGGCTCTTCTGTTTGCTTTCCTTGCTGTATCTCTTGTCAAGGCATTTGCACCTT ATGCCTGTGGTTCTGGAATCCCTGAGATCAAAACCATCCTGAGTGGTTTCATTATTAGAGGCTATCTGGG TAAGTGGACCCTTGTCATCAAAACTATCACTTTGGTGTTGGCGGTGTCATCTGGCTTGAGCCTGGGCAAG GAGGGCCCCCTGGTGCACGTGGCTTGCTGCTGTGGGAACATCTTGTGCCACTGCTTCAACAAATACCGGA AGAATGAAGCCAAGCGGAGAGAGGTCTTATCAGCTGCAGCGGCTGCCGGTGTATCTGTAGCCTTTGGGGC CCCTATTGGTGGAGTATTATTCAGCCTGGAAGAGGTCAGCTACTACTTTCCCCTCAAAACACTATGGCGT TCTTTCTTCGCTGCTCTGGTGGCAGCGTTCACACTCCGCTCCATCAATCCATTCGGAAACAGCCGCTTAG TTCTGTTTTATGTGGAGTTCCACACCCCGTGGCATCTCTTTGAGCTCGTGCCATTCATTGTACTAGGCAT ATTCGGTGGCCTCTGGGGAGCTCTGTTTATCCGCACAAACATTGCCTGGTGTCGGAAGCGTAAAACCACA CAGTTGGGCAAGTATCCTGTCGTCGAGGTACTCATTGTGACGGCCATCACTGCCATCTTGGCTTTCCCCA ATGAATATACCCGGATGAGCACAAGTGAGCTCATTTCTGAGCTGTTCAATGACTGCGGCCTCCTGGACTC CTCCAAGCTCTGTGATTATGAGAACCACTTCAACACAAGCAAAGGCGGTGAGCTGCCTGACAGACCTGCT GGCGTGGGCATCTACAGTGCCATGTGGCAGCTGGCTCTGACACTCATACTGAAAATAGTCATTACTATAT TCACCTTTGGCATGAAGATTCCTTCTGGTCTCTTTATCCCCAGCATGGCTGTCGGTGCTATAGCAGGCCG TCTTTTAGGAGTCGGAATGGAGCAACTGGCTTATTACCACCACGACTGGGGCATCTTCAATAGCTGGTGT AGTCAGGGAGCTGATTGTATCACCCCTGGCCTTTATGCCATGGTTGGGGCTGCTGCCTGCTTAGGTGGGG TGACTCGGATGACTGTTTCTCTTGTTGTCATAATGTTTGAACTGACTGGTGGCCTGGAATATATTGTGCC TCTGATGGCTGCAGCTATGACAAGCAAGTGGGTGGCCGATGCTCTTGGGCGAGAGGGCATTTATGATGCC CACATCCGTCTCAATGGATACCCCTTTCTTGAAGCCAAAGAAGAATTTGCTCATAAGACTCTGGCAATGG ATGTGATGAAGCCCCGGAGAAATGACCCCTTGCTGACTGTCCTTACTCAGGACAGTATGACTGTGGAGGA TGTCGAGACCATAATCAGCGAAACCACGTACAGTGGCTTTCCGGTGGTAGTGTCCCGAGAGTCGCAAAGA CTTGTAGGCTTTGTTCTTCGAAGAGATCTCATTATTTCAATTGAAAATGCTCGGAAGAAACAGGACGGGG TTGTGAGCACTTCCATCATTTATTTCACGGAGCATTCGCCTCCAATGCCTCCATACACCCCACCTACCCT GAAGCTTCGGAACATCCTGGACCTCAGCCCCTTCACTGTGACAGACCTTACGCCAATGGAGATCGTGGTG GACATCTTCCGCAAGCTGGGCCTGCGCCAGTGCCTGGTTACACACAACGGGCGCTTACTTGGAATCATCA CCAAAAAGGATGTGTTAAAGCATATAGCACAGATGGCAAACCAAGACCCTGATTCCATTCTCTTCAACTA G ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001243762
Insert Size	2451 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001243762.1, NP_001230691.1
RefSeq Size	4170 bp
RefSeq ORF	2451 bp
Locus ID	12728
UniProt ID	Q9WVD4
Gene Summary	Proton-coupled chloride transporter. Functions as antiport system and exchanges chloride ions against protons. Important for normal acidification of the endosome lumen. May play an important role in renal tubular function (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) includes two alternate exons in the 5' UTR and 5' CDS, compared to variant 1. It encodes isoform 2, which is longer and has a distinct N-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. The exon combination is inferred based on available transcript data and further supported by similar exon-intron structure of the related human gene, GeneID 1184.

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