Sox6 (NM_001277328) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AI987981; SOX-LZ
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231142 representing NM_001277328 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTTCCAAGCAAGCCACCTCTCCATTTGCCTGTACAGCGGATGGAGAGGAAGCAATGACCCAGGACT TGACCTCAAGGGAAAAGGAAGAGGGCAGTGATCAACACCCGGCCTCCCATCTGCCTCTGCACCCCATAAT GCACAACAAACCTCACTCTGAGGAGCTGCCTACACTTGTCAGTACCATTCAACAAGATGCTGACTGGGAC AGCGTTCTGTCATCTCAGCAAAGAATGGAGTCAGAGAATAATAAGTTATGTTCCCTATATTCCTTCCGAA ATACGTCTACCTCACCACATAAGCCTGACGAAGGGAGTCGGGAGCGTGAAATAATGAACAGTGTTACTTT TGGAACCCCGGAACGCCGCAAAGGGAGCCTTGCCGATGTGGTGGATACGCTGAAGCAGAAGAAGCTGGAG GAGATGACTCGGACAGAACAAGAGGATTCCTCCTGCATGGAAAAACTACTTTCAAAAGATTGGAAGGAAA AAATGGAAAGACTAAATACGAGTGAACTTCTTGGAGAAATCAAAGGTACACCTGAGAGCCTTGCAGAGAA AGAACGGCAACTCTCCACCATGATTACCCAGCTGATCAGCTTGCGGGAGCAGCTCCTGGCAGCCCATGAT GAACAGAAAAAGCTGGCAGCATCGCAGATCGAGAAACAGCGGCAGCAAATGGACCTTGCTCGCCAACAGC AAGAACAGATCGCAAGACAACAGCAGCAACTTCTACAGCAGCAGCACAAGATTAATCTCCTGCAGCAACA GATCCAGGTTCAGGGGCACATGCCTCCGCTCATGATCCCAATTTTTCCACATGACCAGCGGACCTTGGCA GCAGCTGCTGCTGCCCAACAGGGATTCCTCTTCCCCCCTGGAATAACATACAAGCCAGGTGATAACTACC CCGTACAGTTCATTCCGTCAACAATGGCAGCTGCTGCTGCTTCTGGACTCAGCCCTTTACAGCTTCAGCA GCTCTATGCCGCTCAGCTGGCCAGCATGCAGGTGTCACCTGGAGCAAAGATGCCATCAACTCCACAGCCA CCAAACTCGGCAGGGGCAGTCTCACCTACTGGGATAAAAAATGAAAAGAGAGGGACCAGCCCTGTAACTC AAGTTAAGGATGAAACAACAGCCCAGCCGCTGAATCTCTCATCCCGGCCTAAGACAGCAGAGCCTGTGAA GTCCCCAACATCTCCCACCCAGAACCTCTTCCCAGCCAGCAAAACCAGCCCTGTCAACCTGCCAAACAAA AGCAGCATCCCCAGCCCCATTGGAGGAAGCCTGGGGAGAGGATCCTCTCTAGATATACTATCCAGTCTCA ACTCTCCTGCCCTGTTTGGGGACCAGGACACAGTGATGAAAGCCATTCAGGAGGCTCGGAAGATGCGAGA ACAGATCCAGCGGGAGCAACAACAGCAGCCACACGGAGTTGATGGGAAACTGTCCTCCATGAACAACATG GGGCTGAGCAACTGCAGGACTGAGAAGGAAAGAACACGCTTTGAGAACCTGGGTCCCCAATTAACAGGAA AGTCAAGTGAAGATGGAAAACTGGGCCCGGGTGTCATCGATCTCACTCGGCCAGAGGACGCAGAAGGAAG CAAAGCAATGAATGGCTCTGCAGCTAAACTACAGCAGTATTATTGTTGGCCAACAGGAGGCGCCACTGTG GCTGAAGCCCGTGTCTACAGGGATGCCCGCGGCCGTGCCAGCAGCGAACCCCACATCAAGCGACCAATGA ATGCGTTCATGGTTTGGGCAAAGGACGAAAGGAGGAAAATTCTTCAGGCCTTCCCTGACATGCATAACTC CAACATTAGCAAAATCTTAGGATCTCGCTGGAAATCCATGTCCAACCAGGAGAAGCAACCTTACTATGAA GAACAGGCCCGGCTAAGCAAAATCCACCTAGAGAAGTACCCAAACTACAAGTACAAACCCCGGCCGAAGC GCACATGCATCGTGGATGGCAAGAAGCTCCGGATTGGGGAGTACAAGCAACTGATGCGCTCGCGAAGGCA GGAGATGCGACAGTTCTTCACTGTGGGGCAACAGCCTCAGATGCCCATCACCACAGGAACAGGTGTTGTG TATCCTGGTGCTATTACTATGGCAACGACCACACCATCACCTCAGATGACATCTGACTGCTCTAGCACCT CTGCCAGCCCCGAGCCCAGCCTCCCTGTCATCCAGAGCACGTATGGTATGAAGATGGACGGCGCAAGCCT GGCTGGGAACGACATGATTAATGGAGAGGATGAAATGGAAGCCTATGACGACTACGAAGATGATCCCAAA TCAGACTATAGCAGCGAGAATGAGGCCCCAGAGCCTGTCAGTGCCAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231142 representing NM_001277328 Red=Cloning site Green=Tags(s) MSSKQATSPFACTADGEEAMTQDLTSREKEEGSDQHPASHLPLHPIMHNKPHSEELPTLVSTIQQDADWD SVLSSQQRMESENNKLCSLYSFRNTSTSPHKPDEGSREREIMNSVTFGTPERRKGSLADVVDTLKQKKLE EMTRTEQEDSSCMEKLLSKDWKEKMERLNTSELLGEIKGTPESLAEKERQLSTMITQLISLREQLLAAHD EQKKLAASQIEKQRQQMDLARQQQEQIARQQQQLLQQQHKINLLQQQIQVQGHMPPLMIPIFPHDQRTLA AAAAAQQGFLFPPGITYKPGDNYPVQFIPSTMAAAAASGLSPLQLQQLYAAQLASMQVSPGAKMPSTPQP PNSAGAVSPTGIKNEKRGTSPVTQVKDETTAQPLNLSSRPKTAEPVKSPTSPTQNLFPASKTSPVNLPNK SSIPSPIGGSLGRGSSLDILSSLNSPALFGDQDTVMKAIQEARKMREQIQREQQQQPHGVDGKLSSMNNM GLSNCRTEKERTRFENLGPQLTGKSSEDGKLGPGVIDLTRPEDAEGSKAMNGSAAKLQQYYCWPTGGATV AEARVYRDARGRASSEPHIKRPMNAFMVWAKDERRKILQAFPDMHNSNISKILGSRWKSMSNQEKQPYYE EQARLSKIHLEKYPNYKYKPRPKRTCIVDGKKLRIGEYKQLMRSRRQEMRQFFTVGQQPQMPITTGTGVV YPGAITMATTTPSPQMTSDCSSTSASPEPSLPVIQSTYGMKMDGASLAGNDMINGEDEMEAYDDYEDDPK SDYSSENEAPEPVSAN TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001277328
ORF Size	2358 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001277328.1, NP_001264257.1
RefSeq Size	8427 bp
RefSeq ORF	2361 bp
Locus ID	20679
UniProt ID	P40645
MW	87.6 kDa
Gene Summary	This gene encodes a member of a family of transcriptional regulators containing high mobility group (HMG) DNA-binding domains. Function of the encoded protein is important for proper cardiac and skeletal development. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013]

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