Map7 (NM_001198635) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	E-MAP-115; MAP-7; mshi; mste; Mtap7; R75000; ste
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231030 representing NM_001198635 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGCAGGGAGCTGGCGGCGACGGCCACAGGGGCGGCGACGGCGCTACGCACAGCGACCCAGCGT CTGATGGCTATAAAGTACAAGAGAAGAGAACTGCCCCCAGCCGCCCCACTTCTACAGTTTCAGGACAAAC TAGCAACCACTCAGGAAACAAACCAGATCCCCCACCTGTGCTACGGGTTGATGACCGGCAGCGGCTAGCC AGAGAACGCCGAGAGGAACGAGAAAAACAGCTAGCTGCACGAGAAACCGTCTGGCTAGAGAGAGAAGAAC GAGCCCGGCAGCACTATGAGAGACATCTGGAGGCGAGGAAGAAGAAGCTGGAGGACCAACGGCTGAAGGA GGAGCGGAGGAGGGCTGCGGTGGAGGAGAAGCGGAGGCAGAGGCTGGAGGAGGACAAGGAGCGCCATGAA GCTGTTGTCCGACGGACGATGGAAAGGAGCCAGAAGCCAAGGCAGAAGTCTAACCGCTGGTCCTGGGGAA GCCCTCTCCATGGGAGCTCGAGCATCCACAGTGGAGATCCAGACAGGCGCTCAGTTTCCACCATGAATCT TTCGAAACATGTTGATCCTGTCATTAGCAAGCGGCTCTCCTCCTCGTCTGCAACTTTGCTAAACTCTCCA GATAGAGCTCGCCGCCTGCAGCTCAGCCCCTGGGAGAGCAGCGTTGTTAGCAGACTTCTGACGCCCACAC ATTCGTTCCTGGCCAGAAGCAAAAGCACGGCCGCCTTGTCTGGAGACACAGTTATCCCCATTTGTCCTCG TTCAGCATCTTGCAGCCCCATCATCATGCCCTTCAAAGCTGCACACTCTAGAAACCCAGTGGACCGACCA AAACTCTTTGTAACACCGCCTGAGGGCTCTGCACGAAGGAGGACCATTCATGGACTAGCGAGCCATAAAA GAGAGCGAGAAAGAGAACACGTTCCCTTCCACGTTTCCCCGGGCGCCCGCAGGACTCTGTCTCCATCTAA TCTCAAAGCGAGGTCACCGGCTCCAGCCCGCCTTTGGCTCCCATCCAAGTCCATGCCTCATCTGCCTGGT ACTCCCCGGCCTGCATCCTCCTTGCCTCCCGGCTCAGTCAGAGCTGCTTCCGCTCAGGCCCCCTCCTCCT CTCCTGGCAACATCCGGCCTGTCAAGAGAGAAGTGAAAGTGGAGCCTGAGAAGAAAGACCCTTTACCCGC AGTAAAGAGCAGGGTGCCATTAGTGAAGGTAGAGGAGGTCACAGTCGAAGAGGGGACACCCGTGAAGCCA CCTGAGCCTGCTGCTCCAGCCTCGGCCCCCATTGCAACCCCAGCCCCTGCTCCAGCTACGGACCCGGCCC CAGTCCCTGCACCATCATCCACTGTGACTGTCGGTGTAGTTCCTAAGACTTCTGCAGGCACCACCGACCC AGAGGAGGCTACGAGGTTGCTGGCTGAGAAGAGGCGTCTAGCCAGAGAGCAGAGGGAGAAGGAGGAACGG GAGAGGAAGGAAAAGGAAGAGCTGGAGAGACAAAAGATAGAGGAATTGGCCCGTAGGGTGGCTGAAGAGC GAAGTCGCAGGGAAGAAGAAGCCCGCAGGCTGGAAGAGGAGCAGGCTCGAGAGAAAGAGGAGCTGGCGCT GCGTCTGGCTGAGGAGGAGCGGGAGCGGTGGGAGAGGGAGGAGGTGGAGCGCGTGCAGAAGCAGAAAGAA GAAGAAGCCCGAGCCCGGGAGGAGGCAGAGAGGGCTCGGCAGGAACGAGAGAAGCATTTCCAGAAAGAAG AGCAGGAACGGCTGGAGAGGAAGAAGCGACTTGAAGAGATTATGAGAAGAACCAGGAGGACAGAGACCGC TGATAAGAAAACCACTGAGCAAAGAAATGGTGACATAGCCAAAGGAGTTCTCACTGGAGAGCCAGAAGTA CCTGCACTGCCGTGTATGGCCTCTTCAGGAAACGGAGAGTCTGCAGAGAGCCCACATGGAGTCGCTTTAC AGCAATCAGAAGTGACCACAGAGAGTTCTCCAGATTTGGAAAAACAGCCAAATGAAAACGGAATGTCCAT ACAAAATGAAAATTTTGAAGAAGTTATAAACTTACCTGTTGGATCAAAAGCGTCCAGATTAGATGTCACC AATGAGAACCCAGAAATTCCTTTGAAACCAATTTTGGCCTTTAATGATGAAGGGACACTTGGGCCCCTAC CTCAGGTGGATGGTGTGCAGACACAACAGACCGCAGAAGTTATA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231030 representing NM_001198635 Red=Cloning site Green=Tags(s) MAEQGAGGDGHRGGDGATHSDPASDGYKVQEKRTAPSRPTSTVSGQTSNHSGNKPDPPPVLRVDDRQRLA RERREEREKQLAARETVWLEREERARQHYERHLEARKKKLEDQRLKEERRRAAVEEKRRQRLEEDKERHE AVVRRTMERSQKPRQKSNRWSWGSPLHGSSSIHSGDPDRRSVSTMNLSKHVDPVISKRLSSSSATLLNSP DRARRLQLSPWESSVVSRLLTPTHSFLARSKSTAALSGDTVIPICPRSASCSPIIMPFKAAHSRNPVDRP KLFVTPPEGSARRRTIHGLASHKREREREHVPFHVSPGARRTLSPSNLKARSPAPARLWLPSKSMPHLPG TPRPASSLPPGSVRAASAQAPSSSPGNIRPVKREVKVEPEKKDPLPAVKSRVPLVKVEEVTVEEGTPVKP PEPAAPASAPIATPAPAPATDPAPVPAPSSTVTVGVVPKTSAGTTDPEEATRLLAEKRRLAREQREKEER ERKEKEELERQKIEELARRVAEERSRREEEARRLEEEQAREKEELALRLAEEERERWEREEVERVQKQKE EEARAREEAERARQEREKHFQKEEQERLERKKRLEEIMRRTRRTETADKKTTEQRNGDIAKGVLTGEPEV PALPCMASSGNGESAESPHGVALQQSEVTTESSPDLEKQPNENGMSIQNENFEEVINLPVGSKASRLDVT NENPEIPLKPILAFNDEGTLGPLPQVDGVQTQQTAEVI TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001198635
ORF Size	2214 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001198635.1, NP_001185564.1
RefSeq Size	3951 bp
RefSeq ORF	2217 bp
Locus ID	17761
UniProt ID	O88735
MW	82.8 kDa
Gene Summary	Microtubule-stabilizing protein that may play an important role during reorganization of microtubules during polarization and differentiation of epithelial cells. Associates with microtubules in a dynamic manner. May play a role in the formation of intercellular contacts. Colocalization with TRPV4 results in the redistribution of TRPV4 toward the membrane and may link cytoskeletal microfilaments.[UniProtKB/Swiss-Prot Function]

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