Rps6ka1 (NM_001285505) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Mapkapk-1a; p90-Rsk1; p90rsk; p90Rsk1; p90S6K; Rsk; Rsk-1; Rsk1; S6K-alpha-1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230989 representing NM_001285505 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGCTCGCCCAGCTCAAGGAGCCCTGGCCGCTCATGGAGCTGGTGCCGCTGGACCCGGAGAATGGCC AGACTTCAGGGGAAGAAGCTGGACTTCAGCCATCCAAGGATGAGGCCATCCTCAAGGAGATCTCCATCAC ACACCACGTCAAGGCTGGCTCTGAGAAGGCCGATCCATCCCAGTTTGAGCTCCTCAAGGTTCTGGGCCAA GGATCCTTTGGCAAAGTCTTCCTGGTACGCAAGGTCACCCGGCCTGACAGTGGGCACTTGTATGCCATGA AAGTATTAAAGAAGGCCACGCTGAAAGTGCGTGACCGTGTTCGGACCAAGATGGAGAGAGACATCCTCGC TGACGTGAACCACCCGTTCGTGGTGAAGCTACATTATGCCTTCCAGACCGAGGGCAAGCTCTATCTTATT CTGGACTTCCTGCGTGGTGGAGACCTGTTCACACGGCTCTCAAAGGAGGTCATGTTTACAGAGGAGGATG TGAAGTTTTACCTGGCTGAGCTGGCACTGGGCCTGGACCACCTGCACAGCTTGGGCATTATTTACAGAGA CCTCAAGCCTGAGAATATCCTTTTGGATGAGGAGGGCCACATCAAACTCACTGACTTTGGCCTGAGCAAG GAGGCCATAGACCATGAGAAGAAGGCTTACTCCTTCTGCGGGACAGTGGAGTACATGGCGCCCGAGGTTG TCAACCGCCAGGGTCACACCCACAGTGCAGACTGGTGGTCCTATGGGGTGCTGATGGGGAAGGACCGGAA GGAGACCATGACCTTGATTTTGAAGGCGAAGCTAGGCATGCCCCAGTTTCTGAGCACGGAAGCCCAGAGC CTCCTGCGGGCCCTGTTCAAGAGGAATCCTGCCAATCGGCTTGGCTCGGGCCCTGATGGGGCAGAGGAAA TTAAGAGACATATCTTCTACTCCACCATTGACTGGAATAAGCTCTACCGGCGTGAGATCAAGCCCCCTTT CAAGCCGGCTGTGGCCCAGCCCGACGACACCTTCTACTTTGATACCGAGTTCACGTCACGCACACCCAGG GATTCGCCAGGCATCCCTCCCAGTGCTGGTGCCCATCAGCTGTTCCGTGGCTTCAGCTTCGTGGCCACTG GTCTGATGGAGGACGACGGCAAGCCTCGGACCACTCAGGCCCCCCTACACTCGGTGGTACAGCAACTCCA CGGGAAGAACTTGGTTTTCAGTGACGGCTACGTAGTAAAGGAGACGATCGGCGTGGGCTCCTACTCTGTG TGTAAGCGTTGTGTCCACAAGGCCACCAACATGGAGTATGCTGTCAAGGTCATCGACAAGAGCAAAAGAG ATCCCTCAGAAGAGATTGAGATTCTTCTGCGGTATGGCCAGCACCCCAACATCATCACCCTGAAAGATGT GTACGATGATGGTAAGCACGTGTACCTGGTGACAGAGCTGATGAGGGGCGGCGAGCTGCTGGATAAGATC CTACGGCAGAAATTCTTCTCAGAGCGGGAGGCCAGCTTCGTCCTGCACACGATCAGCAAGACTGTGGAAT ACTTGCACTCTCAAGGGGTTGTCCACAGAGATCTCAAACCCAGTAACATCCTCTATGTGGATGAGTCTGG GAACCCCGAGTGCCTGCGCATCTGCGACTTTGGCTTTGCCAAGCAGCTACGGGCCGAGAACGGACTCCTC ATGACACCTTGCTACACAGCCAACTTTGTGGCACCTGAGGTGCTGAAGCGCCAGGGCTACGATGAAGGCT GTGACATATGGAGCCTGGGCATTCTGCTGTACACGATGCTGGCAGGATACACTCCATTTGCCAATGGGCC CAGTGACACCCCAGAGGAGATCCTCACCCGGATCGGCAGCGGGAAGTTCACCCTCAGTGGGGGAAACTGG AACACGGTTTCAGAGACAGCCAAGGACTTGGTATCTAAGATGCTGCATGTGGACCCCCACCAGCGCCTCA CAGCCAAGCAGGTCCTGCAGCACCCATGGATCACCCAGAAAGACAAGCTTCCCCAGAGCCAGTTGTCCCA CCAAGACCTGCAGCTCGTGAAGGGAGCCATGGCGGCTACATACTCTGCTCTCAATAGCTCCAAACCCACC CCTCAGCTCAAGCCAATTGAGTCGTCTATCCTGGCCCAGCGGCGGGTGAGGAAGCTGCCATCCACCACCC TG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230989 representing NM_001285505 Red=Cloning site Green=Tags(s) MPLAQLKEPWPLMELVPLDPENGQTSGEEAGLQPSKDEAILKEISITHHVKAGSEKADPSQFELLKVLGQ GSFGKVFLVRKVTRPDSGHLYAMKVLKKATLKVRDRVRTKMERDILADVNHPFVVKLHYAFQTEGKLYLI LDFLRGGDLFTRLSKEVMFTEEDVKFYLAELALGLDHLHSLGIIYRDLKPENILLDEEGHIKLTDFGLSK EAIDHEKKAYSFCGTVEYMAPEVVNRQGHTHSADWWSYGVLMGKDRKETMTLILKAKLGMPQFLSTEAQS LLRALFKRNPANRLGSGPDGAEEIKRHIFYSTIDWNKLYRREIKPPFKPAVAQPDDTFYFDTEFTSRTPR DSPGIPPSAGAHQLFRGFSFVATGLMEDDGKPRTTQAPLHSVVQQLHGKNLVFSDGYVVKETIGVGSYSV CKRCVHKATNMEYAVKVIDKSKRDPSEEIEILLRYGQHPNIITLKDVYDDGKHVYLVTELMRGGELLDKI LRQKFFSEREASFVLHTISKTVEYLHSQGVVHRDLKPSNILYVDESGNPECLRICDFGFAKQLRAENGLL MTPCYTANFVAPEVLKRQGYDEGCDIWSLGILLYTMLAGYTPFANGPSDTPEEILTRIGSGKFTLSGGNW NTVSETAKDLVSKMLHVDPHQRLTAKQVLQHPWITQKDKLPQSQLSHQDLQLVKGAMAATYSALNSSKPT PQLKPIESSILAQRRVRKLPSTTL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001285505
ORF Size	2172 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001285505.1, NP_001272434.1
RefSeq Size	3118 bp
RefSeq ORF	2175 bp
Locus ID	20111
UniProt ID	P18653
MW	82 kDa
Gene Summary	Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77, regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1. In fibroblast, is required for EGF-stimulated phosphorylation of CREB1, which results in the subsequent transcriptional activation of several immediate-early genes. In response to mitogenic stimulation (EGF and PMA), phosphorylates and activates NR4A1/NUR77 and ETV1/ER81 transcription factors and the cofactor CREBBP. Upon insulin-derived signal, acts indirectly on the transcription regulation of several genes by phosphorylating GSK3B at 'Ser-9' and inhibiting its activity. Phosphorylates RPS6 in response to serum or EGF via an mTOR-independent mechanism and promotes translation initiation by facilitating assembly of the pre-initiation complex. In response to insulin, phosphorylates EIF4B, enhancing EIF4B affinity for the EIF3 complex and stimulating cap-dependent translation. Is involved in the mTOR nutrient-sensing pathway by directly phosphorylating TSC2 at 'Ser-1798', which potently inhibits TSC2 ability to suppress mTOR signaling, and mediates phosphorylation of RPTOR, which regulates mTORC1 activity and may promote rapamycin-sensitive signaling independently of the PI3K/AKT pathway. Mediates cell survival by phosphorylating the pro-apoptotic proteins BAD and DAPK1 and suppressing their pro-apoptotic function. Promotes the survival of hepatic stellate cells by phosphorylating CEBPB in response to the hepatotoxin carbon tetrachloride (CCl4). Mediates induction of hepatocyte prolifration by TGFA through phosphorylation of CEBPB (PubMed:10635333). Is involved in cell cycle regulation by phosphorylating the CDK inhibitor CDKN1B, which promotes CDKN1B association with 14-3-3 proteins and prevents its translocation to the nucleus and inhibition of G1 progression (By similarity). Phosphorylates EPHA2 at 'Ser-897', the RPS6KA-EPHA2 signaling pathway controls cell migration (By similarity).[UniProtKB/Swiss-Prot Function]

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