Pigq (NM_001291025) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Gpi1; Gpi1h; Gpi1p; Gpih
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230765 representing NM_001291025 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGCTCAAGGTCTTCTTCCCCACGTGCTGTGCCTCGGCAGACAGTGGCCTGTTAGTGGGACGATGGG TTCCAGGACAGAGCAGTGCTGTGATCCTGGCCGTGGTGCACTTTCCCTTCATCCCCATCCAAGTCAAGGA GCTCCTGGCCCAGGTGCAGAAGGCCAGTCAAGTACCAGTGGCTGTGCTAGGGACCTGGTGCCACCGTCAG CAGGAACCGCAGGAGAGCCTGGGGAATTTCCTGGAGGGCCTGGGCACCATCTTCTCCCATGACCCCTGGT TGCAGCTCTGCCGGGAGAGGGGTACCAGACTCTGGAGCTGCAAGGCCACTTACCCGCAGATGTCTAACCC TCTGGACATGCACCCCGAAGAGCAGGTCATGCTCATCTTCTATGATCAGCGCAAGCTGCTGCTCTCCTGG TTGCATCCGCCTCCAGTCCTGCCTGCCTGCCAGATGGGAGACACCACAGCCAGCACCGGAGGCCTAGCTG ACATCTTTGACACAGTGGCACGCAGCGAGGTGCTCTTCCGAAATGACCAATTTGATGAGAGACCCGTACG CCTGAGCCACTGGCAATCAGAGGGAGTGGAGGCCAGCATACTTGTGGAGTTGGCAAAGCGGGCCTCTGGG CCTGTCTGCCTGCTGCTGGCTTCTCTGTTGTCCCTGATCTCAGCAGCTAGTGCTTGCCGGCTATGGAAGC TGTGGCCACTTTCCTTCATCAGAAGCAAGCTCTCCACTTGTGAGCAGCTCCATCACCGGCTGAAGCACCT CTCCTTCATCTTCAGCACGGAGAAAGCCCAGAATCCCATGCAGCTGATGAGGAAGGCCAACATGCTGGTT TCTGTGCTACTGGATGTGGCCCTTGGCTTGCTGCTGCTCTCTTGGCTCCACAGCAATAACCGAATTGGAC AGCTGGCCAATGCCCTGGTCCCTGTGGCTGATAGTCAAAGCAGTGTCTCCGTGGAAGTCTTGTGGGCTCT CTTCTGCTCCGTCAGTGCGGATGCCTGCTGGGTCTGTTTGTTGCCAGGCCAGGAATCTGGGGCAGCAGTG GGCTCCTCTCAGCTTTGTGAGATCTGTGGAGGTCATCCCAGTAGGAAAGAGGAGCTGGAGCTGGGTGAAC GGCGCGTGGCTGAGGAGCTCCAGCATCTGCTGCAGTGGCTGATGGGTGCTCCTGCTGGGCTCAAGATGAA TCGGGCACTGGATCAGGTGCTAGGCCGCTTTTTCCTGTACCACATCCATCTGTGGATAAGCTATATCCAC CTTATGTCCCCCTTTATTGAGCATATCCTGTGGCATGTGGGACTCTCAGCCTGCCTTGGACTGACTGTTG CTCTGTCCATCTTTTCGGATATCATCGCCCTTCTCACCTTCCACATCTATTGCTTCTATGTCTACGGTGC CAGGCTGTACTGCCTGAAGATCTATGGCCTTTCCTCTCTCTGGCGTCTGTTCCGGGGGAAGAAGTGGAAT GTTCTGCGCCAGCGAGTGGATTCTTGTTCCTATGACCTTGACCAGCTCTTCATTGGGACCTTGCTCTTCA CCATCCTGGTCTTCTTGCTACCCACCACCGCTCTGTACTACCTGGTATTCACCCTGCTCCGGCTCCTGGT GATCACTGTGCAAGGCTTAATCCATCTACTTGTAGACCTCATCAATTCCCTGCCACTATACTCCCTTGGC CTTCGACTCTGCCGACCCTACAGGCTGGCAGCTGGTGTGAAGTTCAGAGTTCTGGAAAAGGAGGCAGGCA GACCCCTCCGCCTCCTGATGCAGATAAACCCCCTGTCCTATAACCATGTGATGCACATCTACCGTCTGCC TCGCTGTGGCTGCCATCCCAAGCACTCCTGGGGTACCCTGTGCCGAAAGCTGGTCTTCGGAGAGCTCATC TACCCCTGGAGGCAGAGAGAGGACAAACAGGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230765 representing NM_001291025 Red=Cloning site Green=Tags(s) MVLKVFFPTCCASADSGLLVGRWVPGQSSAVILAVVHFPFIPIQVKELLAQVQKASQVPVAVLGTWCHRQ QEPQESLGNFLEGLGTIFSHDPWLQLCRERGTRLWSCKATYPQMSNPLDMHPEEQVMLIFYDQRKLLLSW LHPPPVLPACQMGDTTASTGGLADIFDTVARSEVLFRNDQFDERPVRLSHWQSEGVEASILVELAKRASG PVCLLLASLLSLISAASACRLWKLWPLSFIRSKLSTCEQLHHRLKHLSFIFSTEKAQNPMQLMRKANMLV SVLLDVALGLLLLSWLHSNNRIGQLANALVPVADSQSSVSVEVLWALFCSVSADACWVCLLPGQESGAAV GSSQLCEICGGHPSRKEELELGERRVAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIH LMSPFIEHILWHVGLSACLGLTVALSIFSDIIALLTFHIYCFYVYGARLYCLKIYGLSSLWRLFRGKKWN VLRQRVDSCSYDLDQLFIGTLLFTILVFLLPTTALYYLVFTLLRLLVITVQGLIHLLVDLINSLPLYSLG LRLCRPYRLAAGVKFRVLEKEAGRPLRLLMQINPLSYNHVMHIYRLPRCGCHPKHSWGTLCRKLVFGELI YPWRQREDKQD TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001291025
ORF Size	1923 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001291025.1, NP_001277954.1
RefSeq Size	3295 bp
RefSeq ORF	1926 bp
Locus ID	14755
MW	73 kDa
Gene Summary	Part of the complex catalyzing the transfer of N-acetylglucosamine from UDP-N-acetylglucosamine to phosphatidylinositol, the first step of GPI biosynthesis.[UniProtKB/Swiss-Prot Function]

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