Sec23b (NM_001281816) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230688 representing NM_001281816 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTCTGAGTCTCCTTCCTCCAGATGCCCTGGTTGGTCTCATCACTTTTGGGAGGATGGTGCAGGTTC ATGAGCTAAGCTGTGAAGGAATCTCCAAGAGCTATGTCTTCCGAGGGACCAAGGATTTAACTGCAAAGCA AATACAGGAGATGCTGGGCCTGACCAAGTCAGCCATGCCTGTGCAGCAAGCCAGACCTGCTCAACCTCAG GAGCAGCCTTTTGTTTCCAGCAGATTTCTACAGCCCATTCACAAGATTGACATGAACCTCACAGATCTTC TCGGGGAGCTGCAGAGGGACCCATGGCCAGTAACTCAGGGGAAGAGACCACTGCGGTCCACTGGTGTTGC TTTGTCCATTGCTGTTGGCTTGTTGGAGGGGACTTTCCCAAATACTGGAGCCAGGATCATGCTGTTCACT GGCGGGCCCCCGACCCAAGGACCTGGCATGGTGGTTGGAGATGAGTTGAAGACTCCTATTCGTTCCTGGC ACGACATTGAGAAAGATAACGCACGATTCATGAAGAAGGCAACCAAGCATTATGAGATGCTTGCTAATCG AACTGCTACAAATGGCCACTGCATTGATATTTATGCTTGTGCCCTTGACCAAACTGGACTTCTGGAGATG AAGTGCTGTCCAAATCTTACAGGAGGCCACATGGTGATGGGAGATTCTTTCAACACTTCTCTTTTCAAGC AGACATTCCAAAGAATTTTTAGTAAAGACTTCAACGGAGATTTCAGAATGGCGTTTGGTGCTACGTTGGA TGTAAAGACCTCTCGGGAACTGAAGATTGCAGGAGCCATTGGTCCATGCGTGTCTCTGAATGTGAAAGGG CCATGCGTGTCAGAAAATGAGCTTGGTGTTGGTGGCACAAGTCAGTGGAAGATCTGTGGCCTGGATCCCT CATCTACTCTTGGCATCTACTTTGAAGTTGTCAATCAGCACAATGCTCCAGTTCCCCAGGGAGGCAGAGG TGCCATCCAGTTTGTCACACAGTATCAGCATTCCAGCACTCAGAAGCGCATCCGAGTGACCACCATTGCC CGCAATTGGGCAGATGCACAGAGTCAGCTGAGGCACATAGAAGCAGCATTTGACCAGGAGGCCGCAGCAG TCCTGATGGCACGGCTTGGAGTGTTCCGCGCAGAGTCAGAGGAGGGGCCTGACGTGCTTCGGTGGCTGGA TCGGCAACTCATCAGACTGTGTCAGAAGTTTGGGCAGTATAACAAAGAAGACCCTACGTCTTTCAGATTG TCAGACTCCTTTTCTCTGTATCCTCAGTTCATGTTCCATCTGAGAAGATCTCCATTTCTTCAAGTATTTA ACAACAGTCCTGATGAGTCCTCCTATTATAGACACCATTTTGCCCGGCAGGATCTGACTCAGTCCCTCAT TATGATCCAGCCCATTCTCTATTCTTACTCCTTCCACGGACCACCAGAGCCTGTACTCCTGGACAGCAGT AGCATTCTAGCTGACAGAATTCTGCTGATGGATACTTTCTTCCAAATTGTCATTTACCTTGGTGAGACCA TAGCCCAGTGGCGGAAGGCTGGATACCAGGACATGCCCGAGTATGAAAACTTCAAGCACCTTCTTCAGGC GCCCCTGGATGATGCCCAAGAAATTCTGCAAGCACGCTTCCCAATGCCACGTTATATCAACACAGAGCAT GGCGGCAGTCAGGCTCGGTTCCTGTTGTCTAAAGTTAACCCATCTCAGACACACAATAACCTGTATGCCT GGGGGCAGGAAACTGGAGCGCCCATCCTGACAGACGATGTCAGCCTACAGGTGTTCATGGATCACCTGAA GAAGCTGGCTGTGTCTAGTGCCTCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230688 representing NM_001281816 Red=Cloning site Green=Tags(s) MSLSLLPPDALVGLITFGRMVQVHELSCEGISKSYVFRGTKDLTAKQIQEMLGLTKSAMPVQQARPAQPQ EQPFVSSRFLQPIHKIDMNLTDLLGELQRDPWPVTQGKRPLRSTGVALSIAVGLLEGTFPNTGARIMLFT GGPPTQGPGMVVGDELKTPIRSWHDIEKDNARFMKKATKHYEMLANRTATNGHCIDIYACALDQTGLLEM KCCPNLTGGHMVMGDSFNTSLFKQTFQRIFSKDFNGDFRMAFGATLDVKTSRELKIAGAIGPCVSLNVKG PCVSENELGVGGTSQWKICGLDPSSTLGIYFEVVNQHNAPVPQGGRGAIQFVTQYQHSSTQKRIRVTTIA RNWADAQSQLRHIEAAFDQEAAAVLMARLGVFRAESEEGPDVLRWLDRQLIRLCQKFGQYNKEDPTSFRL SDSFSLYPQFMFHLRRSPFLQVFNNSPDESSYYRHHFARQDLTQSLIMIQPILYSYSFHGPPEPVLLDSS SILADRILLMDTFFQIVIYLGETIAQWRKAGYQDMPEYENFKHLLQAPLDDAQEILQARFPMPRYINTEH GGSQARFLLSKVNPSQTHNNLYAWGQETGAPILTDDVSLQVFMDHLKKLAVSSAS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001281816
ORF Size	1845 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001281816.1, NP_001268745.1
RefSeq Size	2421 bp
RefSeq ORF	1848 bp
Locus ID	27054
MW	69.3 kDa
Gene Summary	Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules for their transport to the Golgi complex.[UniProtKB/Swiss-Prot Function]

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